Serum AGR2 as an early diagnostic and postoperative prognostic biomarker of human lung adenocarcinoma

2012 ◽  
Vol 10 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Kyukwang Chung ◽  
Noritoshi Nishiyama ◽  
Shotaro Yamano ◽  
Hiroaki Komatsu ◽  
Shoji Hanada ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Human Lung ◽  
Prognostic Biomarker ◽  
Human Lung Adenocarcinoma ◽  
Early Diagnostic

scholarly journals SLC17A9 Is a Novel Prognostic Biomarker Correlated With Immune Infiltrates in Human Lung Adenocarcinoma and Lung Squamous Cell Carcinoma

2021 ◽  
Author(s):  
Yan Gao ◽  
Yi-Jia Chen ◽  
Fuyan Li ◽  
Ruimin Wu ◽  
Daobing Zeng ◽  
...  
Keyword(s):  
Overall Survival ◽  
Squamous Cell Carcinoma ◽  
Lung Adenocarcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Human Lung ◽  
Prognostic Biomarker ◽  
Lung Squamous Cell Carcinoma ◽  
Gene Set Enrichment Analysis ◽  
Human Lung Adenocarcinoma

Abstract Background Overexpression of vesicular nucleotide transporter (SLC17A9) has been found in different types of cancers. Nonetheless, little is known about its influence on lung cancers including human lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Methods Integrative analysis of SLC17A9 and other solute carrier family 17 genes (SLC17A1-8) was performed in patients with LUAD and LUSC based on The Cancer Genome Atlas database. Real-time PCR, western blots, MTS assay, EdU assay, ATP production assays and cell cycle analysis were applied to determine the effect and mechanism of SLC17A9 knockdown in LUAD cells. Results Compared with normal tissue, two SLC17 genes (SLC17A5 and SLC17A9) exhibited a distinctly different expression pattern in LUAD and LUSC. The expression of SLC17A3/7/8/9 expression was significantly correlated with worse overall survival (p < 0.05) in LUAD. Conversely, SLC17A1/2/4/6/9 expression was correlated with poorer OS (p < 0.05) in LUSC. ROC analysis suggested that the area under the curve of most SLC17 family genes was higher than 0.5. Meanwhile, multiple types of genetic alterations in SLC17 family genes were observed in tumor samples. Gene set enrichment analysis GSEA and protein-protein interaction results revealed that oncogenic signaling pathways and biological regulation, metabolic process, hallmark of myc targets, DNA repair, coagulation and complement were linked to SLC17A9 upregulation. Moreover, SLC17A9 knockdown significantly inhibited cell proliferation and ATP levels by affecting Myc, MFN2, STAT3, Cytochrome C and P2X1 expression in A549 cells. Specifically, SLC17A9 expression correlated negatively with overall survival and positively with most LUSC immune cells. SLC17A9 expression has correlations with infiltrating levels of B cells, CD4 + T cells, M1 macrophages, natural killer cells, Th1, Th2, Tfh, Th17 and Treg cells, as well as PD-1, CTLA4, and LAG3 of T cell exhaustion in LUAD. Conclusions Together, SLC17A9 could potentially serve as a prognostic biomarker correlated with immune infiltrates in LUAD and LUSC.

scholarly journals Identification of integrin β1 as a prognostic biomarker for human lung adenocarcinoma using 2D-LC-MS/MS combined with iTRAQ technology

2013 ◽  
Vol 30 (1) ◽  
pp. 341-349 ◽  
Author(s):  
PENG-FEI ZHANG ◽  
GU-QING ZENG ◽  
LUN-ZHAO YI ◽  
JIAN-PING LIU ◽  
XUN-XUN WAN ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Human Lung ◽  
Prognostic Biomarker ◽  
Integrin Β1 ◽  
Human Lung Adenocarcinoma

FOX-A1 Contributes to Acquisition of Chemoresistance in Human Lung Adenocarcinoma via Transactivation of SOX5

2019 ◽  
Author(s):  
Dongqin Chen ◽  
Rui Wang ◽  
Chen Yu ◽  
Fei Cao ◽  
Xuefeng Zhang ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Human Lung ◽  
Human Lung Adenocarcinoma

scholarly journals Antimigratory Effects of the Methanol Extract fromMomordica charantiaon Human Lung Adenocarcinoma CL1 Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Hsue-Yin Hsu ◽  
Jung-Hsuan Lin ◽  
Chia-Jung Li ◽  
Shih-Fang Tsang ◽  
Chun-Hao Tsai ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Human Lung ◽  
Human Cancer ◽  
Momordica Charantia ◽  
Invasive Ability ◽  
Human Cancer Cells ◽  
Human Lung Adenocarcinoma ◽  
Significant Difference

Momordica charantiahas been found to exhibit anticancer activity, in addition to its well-known therapeutic functions. We have demonstrated that the leaf extract ofMomordica charantia(MCME) induces apoptosis in several human cancer cells through caspase- and mitochondria-dependent pathways. In this study, a different susceptibility to MCME was found in human lung adenocarcinoma CL1 cells with different metastatic ability, leading to the significant difference of cell viability and invasiveness between MCME-treated CL1-0 and CL1-5 cells. MCME was found to upregulate the expression of Wnt-2 and affect the migratory and invasive ability of CL1 cells through suppressed MMP-2 and MMP-9 enzymatic activities. We proposed that MCME mediates inhibition against migration of CL1 cells by reducing the expression and activation of Src and FAK to decrease the expression of downstream Akt,β-catenin, and MMPs.

Sign in / Sign up

Export Citation Format

Share Document