Bortezomib could down-regulate the expression of RANKL, inhibit cell proliferation and induce cell apoptosis in the human myeloma cell line RPMI 8226 by activating casepase-3

2017 ◽  
Vol 20 (2) ◽  
pp. 217-224 ◽  
Author(s):  
Li Lin ◽  
Dong Chen ◽  
Zhen-Fei Xiang ◽  
Ren-Zhi Pei ◽  
Pi-Sheng Zhang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Cell Apoptosis ◽  
Myeloma Cell ◽  
Myeloma Cell Line ◽  
Human Myeloma Cell Line ◽  
Induce Cell Apoptosis ◽  
Rpmi 8226 ◽  
Inhibit Cell Proliferation ◽  
Human Myeloma Cell

Wogonin induces apoptosis in RPMI 8226, a human myeloma cell line, by downregulating phospho-Akt and overexpressing Bax

Life Sciences ◽  
2013 ◽  
Vol 92 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Meng Zhang ◽  
Li-Ping Liu ◽  
Yuling Chen ◽  
Xiao-ying Tian ◽  
Jian Qin ◽  
...  
Keyword(s):  
Cell Line ◽  
Myeloma Cell ◽  
Myeloma Cell Line ◽  
Human Myeloma Cell Line ◽  
Rpmi 8226 ◽  
Human Myeloma Cell

scholarly journals Evidence for the Involvement of Both Retinoic Acid Receptor- and Retinoic X Receptor-Dependent Signaling Pathways in the Induction of Tissue Transglutaminase and Apoptosis in the Human Myeloma Cell Line RPMI 8226

Blood ◽  
1998 ◽  
Vol 91 (7) ◽  
pp. 2423-2432 ◽  
Author(s):  
Bertrand Joseph ◽  
Olga Lefebvre ◽  
Claude Méreau-Richard ◽  
Pierre-Marie Danzé ◽  
Marie-Thérèse Belin-Plancot ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Retinoic Acid ◽  
Cell Line ◽  
Retinoic Acid Receptor ◽  
Tissue Transglutaminase ◽  
Myeloma Cell ◽  
Myeloma Cell Line ◽  
Human Myeloma Cell Line ◽  
Rpmi 8226 ◽  
Human Myeloma Cell

In this study, we show that both all-trans-retinoic acid (atRA) and 9-cis-retinoic acid (9-cis-RA) are potent inducers of tissue transglutaminase (TGase II), an enzyme involved in apoptosis, at the level of both enzyme activity and mRNA in the human myeloma cell line RPMI 8226. RPMI 8226 cells were shown to express mRNAs for all the retinoid receptors subtypes, ie, RARα, RARβ, RARγ, RXRα, RXRβ, and RXRγ. To identify which of these receptors are involved in regulating TGase II expression, several receptor-selective synthetic retinoids were used. Neither CD367, a very potent retinoid that selectively binds and activates receptors of the RAR family, nor CD2425, an RXR-selective agonist, induced TGase II when used alone. However, combination of CD367 and CD2425 resulted in nearly full induction of the enzyme. Moreover, when used in combination with atRA, CD367 partially inhibited the atRA-dependent induction of TGase II, whereas CD2425 enhanced it. The effects of Am 580, CD417, and CD437, three synthetic retinoids selective for the RARs subtypes RARα, RARβ, and RARγ, respectively, were also investigated. None of these compounds was able to induce TGase II when used alone; however, the combination of each of them with CD2425 resulted in strong induction of the enzyme activity, reaching 30% to 50% of the values obtained in the presence of retinoic acid and suggesting functional redundancy between the RAR subtypes. Finally, treatment with atRA or the combination of CD367 and CD2425, but not with CD367 or CD2425 alone, was also shown to trigger apoptosis in RPMI 8226 cells, with prominent accumulation of TGase II immunoreactivity in apoptotic cells. Taken together these data suggest that the induction of TGase II expression and apoptosis in the RPMI 8226 myeloma cell line required ligand-dependent activation of both the RAR and RXR receptors.

scholarly journals Evidence for the Involvement of Both Retinoic Acid Receptor- and Retinoic X Receptor-Dependent Signaling Pathways in the Induction of Tissue Transglutaminase and Apoptosis in the Human Myeloma Cell Line RPMI 8226

Blood ◽  
1998 ◽  
Vol 91 (7) ◽  
pp. 2423-2432 ◽  
Author(s):  
Bertrand Joseph ◽  
Olga Lefebvre ◽  
Claude Méreau-Richard ◽  
Pierre-Marie Danzé ◽  
Marie-Thérèse Belin-Plancot ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Retinoic Acid ◽  
Cell Line ◽  
Retinoic Acid Receptor ◽  
Tissue Transglutaminase ◽  
Myeloma Cell ◽  
Myeloma Cell Line ◽  
Human Myeloma Cell Line ◽  
Rpmi 8226 ◽  
Human Myeloma Cell

AbstractIn this study, we show that both all-trans-retinoic acid (atRA) and 9-cis-retinoic acid (9-cis-RA) are potent inducers of tissue transglutaminase (TGase II), an enzyme involved in apoptosis, at the level of both enzyme activity and mRNA in the human myeloma cell line RPMI 8226. RPMI 8226 cells were shown to express mRNAs for all the retinoid receptors subtypes, ie, RARα, RARβ, RARγ, RXRα, RXRβ, and RXRγ. To identify which of these receptors are involved in regulating TGase II expression, several receptor-selective synthetic retinoids were used. Neither CD367, a very potent retinoid that selectively binds and activates receptors of the RAR family, nor CD2425, an RXR-selective agonist, induced TGase II when used alone. However, combination of CD367 and CD2425 resulted in nearly full induction of the enzyme. Moreover, when used in combination with atRA, CD367 partially inhibited the atRA-dependent induction of TGase II, whereas CD2425 enhanced it. The effects of Am 580, CD417, and CD437, three synthetic retinoids selective for the RARs subtypes RARα, RARβ, and RARγ, respectively, were also investigated. None of these compounds was able to induce TGase II when used alone; however, the combination of each of them with CD2425 resulted in strong induction of the enzyme activity, reaching 30% to 50% of the values obtained in the presence of retinoic acid and suggesting functional redundancy between the RAR subtypes. Finally, treatment with atRA or the combination of CD367 and CD2425, but not with CD367 or CD2425 alone, was also shown to trigger apoptosis in RPMI 8226 cells, with prominent accumulation of TGase II immunoreactivity in apoptotic cells. Taken together these data suggest that the induction of TGase II expression and apoptosis in the RPMI 8226 myeloma cell line required ligand-dependent activation of both the RAR and RXR receptors.

scholarly journals Activation of murine 'T' lymphomas in the presence of a human myeloma cell line, RPMI-8226, in vivo

1988 ◽  
Vol 57 (3) ◽  
pp. 290-292 ◽  
Author(s):  
BC Millar ◽  
JL Millar ◽  
A Jones ◽  
SW Feary ◽  
D Robertson ◽  
...  
Keyword(s):  
Cell Line ◽  
Myeloma Cell ◽  
Myeloma Cell Line ◽  
Human Myeloma Cell Line ◽  
T Lymphomas ◽  
Rpmi 8226 ◽  
Human Myeloma Cell

scholarly journals Biological evaluation of both enantiomers of fluoro-thalidomide using human myeloma cell line H929 and others

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0182152 ◽  
Author(s):  
Etsuko Tokunaga ◽  
Hidehiko Akiyama ◽  
Vadim A. Soloshonok ◽  
Yuki Inoue ◽  
Hideaki Hara ◽  
...  
Keyword(s):  
Cell Line ◽  
Myeloma Cell ◽  
Biological Evaluation ◽  
Myeloma Cell Line ◽  
Human Myeloma Cell Line ◽  
Human Myeloma Cell

Human myeloma cell line carrying a Philadelphia chromosome

Science ◽  
1982 ◽  
Vol 216 (4549) ◽  
pp. 997-999 ◽  
Author(s):  
A Karpas ◽  
P Fischer ◽  
D Swirsky
Keyword(s):  
Cell Line ◽  
Philadelphia Chromosome ◽  
Myeloma Cell ◽  
Myeloma Cell Line ◽  
Human Myeloma Cell Line ◽  
Human Myeloma Cell

Abstract 400: Establishment and characterization of a novel IL6-dependent HLA-A2+ human myeloma cell line LNT1

2010 ◽  
Author(s):  
Sun-Young Kong ◽  
Weihua Song ◽  
Xian-Feng Li ◽  
Sabikun Nahar ◽  
Paola Dal Cin ◽  
...  
Keyword(s):  
Cell Line ◽  
Myeloma Cell ◽  
Myeloma Cell Line ◽  
Human Myeloma Cell Line ◽  
Human Myeloma Cell

scholarly journals Aminoacyl Transfer RNA Profiles in Human Myeloma Cells

Blood ◽  
1971 ◽  
Vol 38 (2) ◽  
pp. 246-252 ◽  
Author(s):  
SEITOKU FUJIOKA ◽  
ROBERT C. GALLO
Keyword(s):  
Cell Line ◽  
Peripheral Blood ◽  
Myeloma Cell ◽  
Bence Jones Protein ◽  
Myeloma Cell Line ◽  
Reverse Phase ◽  
Normal Peripheral Blood ◽  
Human Myeloma Cell Line ◽  
Aminoacyl Transfer ◽  
Rpmi 8226

Abstract Comparative studies of aspartyl-, leucyl-, seryl- and tyrosyl-tRNA by a reverse phase column co-chromatography have been performed between a human normal lymphoblast cell line (RPMI 1788) and a human myeloma cell line (RPMI 8226). The cell lines were derived from normal peripheral blood and peripheral blood of a patient with multiple myeloma and produce IgM and lambda-type light chain (Bence Jones protein), respectively. Similar chromatographic patterns were obtained for leucyl- and seryl-tRNA. Minor differences in aspartyl-tRNA and larger differences in tyrosyl-tRNA were observed.

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