In vitro evaluation of collagen immobilization on polytetrafluoroethylene through NH3 plasma treatment to enhance endothelial cell adhesion and growth

2017 ◽  
Vol 28 (5) ◽  
pp. 489-501 ◽  
Author(s):  
Mahboobeh Mahmoodi ◽  
Mohammad Zamanifard ◽  
Mina Safarzadeh ◽  
Shahin Bonakdar
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Plasma Treatment ◽  
In Vitro Evaluation ◽  
Collagen Immobilization ◽  
Endothelial Cell Adhesion

Enhanced Vascular Endothelial Cell Function on Nanostructured Titanium Surface Features: The Role of Nano to Submicron Roughness

2008 ◽  
Vol 1136 ◽  
Author(s):  
Jing Lu ◽  
Dongwoo Khang ◽  
Thomas J. Webster
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Cell Function ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Endothelial Cell Function ◽  
Titanium Surfaces ◽  
Endothelial Cell Adhesion

ABSTRACTTo study the contribution of different surface feature properties in improving vascular endothelial cell adhesion, rationally designed nano/sub-micron patterns with various dimensions were created on titanium surfaces in this study. In vitro results indicated that endothelial cell adhesion was improved when the titanium pattern dimensions decreased into the nano-scale. Specifically, endothelial cells preferred to adhere on sub-micron and nano rough titanium substrates compared to flat titanium. Moreover, titanium with nano and sub-micron roughness and with the same chemistry as compared to flat titanium, had significantly greater surface energy. Thus, the present study indicated the strong potential of surface nanotopography and nano/sub-micron roughness for improving current vascular stent design.

The anti-ischemic drugs defibrotide and oligotide analogously inhibit leukocyte-endothelial cell adhesion in vitro

1996 ◽  
pp. 420-424
Author(s):  
F. Pellegatta ◽  
E. Ferrero ◽  
A. Marni ◽  
S. Chierchia ◽  
D. Forti ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Endothelial Cell Adhesion

Human Intervertebral Disc Aggrecan Inhibits Endothelial Cell Adhesion and Cell Migration In Vitro

Spine ◽  
2005 ◽  
Vol 30 (10) ◽  
pp. 1139-1147 ◽  
Author(s):  
William E. B. Johnson ◽  
Bruce Caterson ◽  
Stephen M. Eisenstein ◽  
Sally Roberts
Keyword(s):  
Cell Migration ◽  
Cell Adhesion ◽  
Endothelial Cell ◽  
Intervertebral Disc ◽  
Endothelial Cell Adhesion

DOCOSAHEXAENOIC AND EICOSAPENTAENOIC ACIDS INHIBIT IN VITRO HUMAN LYMPHOCYTE-ENDOTHELIAL CELL ADHESION

1996 ◽  
Vol 62 (11) ◽  
pp. 1649-1657 ◽  
Author(s):  
Bachir Khalfoun ◽  
Gilles Thibault ◽  
Pierre Bardos ◽  
Yvon Lebranchu
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Human Lymphocyte ◽  
Endothelial Cell Adhesion

scholarly journals Association of carbamylated high-density lipoprotein with coronary artery disease in type 2 diabetes mellitus: carbamylated high-density lipoprotein of patients promotes monocyte adhesion

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Zhongli Chen ◽  
Song Ding ◽  
Yan Ping Wang ◽  
Liang Chen ◽  
Jing Yan Mao ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Control Group ◽  
Diabetic Patients ◽  
Artery Disease ◽  
Endothelial Cell Adhesion

Abstract Background Increasing evidence showed that carbamylated lipoprotein accelerated atherosclerosis. However, whether such modification of high-density lipoprotein (HDL) particles alters in type 2 diabetes mellitus (T2DM) patients and facilitates vascular complications remains unclear. We aimed to investigate the alteration of the carbamylation in HDL among T2DM patients and clarify its potential role in atherogenesis. Methods A total of 148 consecutive T2DM patients undergoning angiography and 40 age- and gender-matched control subjects were included. HDL was isolated from plasma samples, and the concentration of HDL carbamyl-lysine (HDL-CBL) was measured. Furthermore, the HDL from subjects and in-vitro carbamylated HDL (C-HDL) was incubated with endothelial cells and monocyte to endothelial cell adhesion. Adhesion molecule expression and signaling pathway were detected. Results Compared with the control group, the HDL-CBL level was remarkably increased in T2DM patients (6.13 ± 1.94 vs 12.00 ± 4.06 (ng/mg), P < 0.001). Of note, HDL-CBL demonstrated a more significant increase in T2DM patients with coronary artery disease (CAD) (n = 102) than those without CAD (n = 46) (12.75 ± 3.82 vs. 10.35 ± 4.11(ng/mg), P = 0.001). Multivariate logistic regression analysis demonstrated that higher HDL-CBL level was independently associated with a higher prevalence of CAD in diabetic patients after adjusting for established cofounders (adjusted odds ratio 1.174, 95% confidence Interval 1.045–1.319, p = 0.017). HDL from diabetic patients with CAD enhanced greater monocyte adhesion than that from the non-CAD or the control group (P < 0.001). Such pro-atherogenic capacity of diabetic HDL positively correlated with HDL-CBL level. Furthermore, in-vitro incubation of carbamylated HDL (C-HDL) with endothelial promoted monocyte to endothelial cell adhesion, induced upregulation of cell adhesion molecules expression, and activated NF-κB/p65 signaling in endothelial cells. Inhibiting carbamylation of HDL or NF-κB activation attenuated the monocyte to endothelial cell adhesion and cell surface adhesion molecules expression. Conclusions Our study identified elevated carbamylation modification of HDL from T2DM patients, especially in those with concomitant CAD. We also evidenced that C-HDL enhanced monocyte to endothelial cell adhesion, indicating a potential pro-atherogenic role of C-HDL in atherosclerosis among T2DM patients. Trial registrationhttps://register.clinicaltrials.gov, NCT04390711 Registered on 14 May 2020; Retrospectively registered

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