scholarly journals Comparative adhesive and migratory properties of mesenchymal stem cells from different tissues

Biorheology ◽  
2019 ◽  
Vol 56 (1) ◽  
pp. 15-30 ◽  
Author(s):  
Asma Alanazi ◽  
Hafsa Munir ◽  
Mohammed Alassiri ◽  
Lewis S.C. Ward ◽  
Helen M. McGettrick ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Different Tissues

Comparing the Chondrogenic Potential in vivo of Autogeneic Mesenchymal Stem Cells Derived from Different Tissues

2010 ◽  
Vol 39 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Qiang Li ◽  
Jicun Tang ◽  
Riying Wang ◽  
Chaoyong Bei ◽  
Linwei Xin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Potential ◽  
Different Tissues

Characteristics of mesenchymal stem cells derived from different tissues of goat

2019 ◽  
Vol 43 (3) ◽  
pp. 107-114
Author(s):  
Na-Yeon Gu ◽  
◽  
Da-Un Jeong ◽  
Jeong Su Byeon ◽  
Jae-Young Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Different Tissues

scholarly journals Mesenchymal Stem Cells from Different Tissues: Immune Status and Activity

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
Trivanović D ◽  
Krstić J ◽  
Mojsilović S ◽  
Djordjević IO ◽  
Ilić V ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Immune Status ◽  
Different Tissues

scholarly journals Comparison of biological features of wild European rabbit mesenchymal stem cells derived from different tissues.

2021 ◽  
Author(s):  
Marta Díaz-de Frutos ◽  
Alexandra Calle ◽  
María Zamora-Ceballos ◽  
Juan Bárcena ◽  
Esther Blanco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
European Rabbit ◽  
Isolation And Characterization ◽  
Expression Characteristic ◽  
Subcutaneous Adipose ◽  
Different Tissues

Although the European rabbit is an "endangered" species and a notorious biological model, the analysis and comparative characterization of new tissue sources of rabbit mesenchymal stem cells (rMSCs) has not been well studied. Here we report for the first time the isolation and characterization of rMSCs derived from an animal belonging to a natural rabbit population within the species native region. New rMSC lines were isolated from different tissues: oral mucosa (rOM-MSC), dermal skin (rDS-MSC), subcutaneous adipose tissue (rSCA-MSC), ovarian adipose tissue (rOA-MSC), oviduct (rO-MSC), and mammary gland (rMG­MSC). The six rMSC lines showed plastic adhesion with fibroblast-like morphology and were all shown to be positive for CD44 and CD29 expression (characteristic markers of MSCs), and negative for CD34 or CD45 expression. In terms of pluripotency features, all rMSC lines expressed NANOG, OCT4, and SOX2. Furthermore, all rMSC lines cultured under osteogenic, chondrogenic, and adipogenic conditions showed differentiation capacity. In conclusion, this study describes the isolation and characterization of new rabbit cell lines from different tissue origins, with a clear mesenchymal pattern. We show that rMSC do not exhibit differences in terms of morphological features, expression of the cell surface, and intracellular markers of pluripotency and in vitro differentiation capacities, attributable to their tissue of origin.

scholarly journals Characterization of Human Mesenchymal Stem Cells from Different Tissues and Their Membrane Encasement for Prospective Transplantation Therapies

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Eva Schmelzer ◽  
Daniel T. McKeel ◽  
Jörg C. Gerlach
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Cell Fusion ◽  
Human Mesenchymal Stem Cells ◽  
Surface Markers ◽  
Multilineage Differentiation ◽  
Differentiation Potential ◽  
Donor Cells ◽  
Different Tissues

Human mesenchymal stem cells can be isolated from various organs and are in studies on therapeutic cell transplantation. Positive clinical outcomes of transplantations have been attributed to both the secretion of cytokines and growth factors as well as the fusion of donor cells with that of the host. We compared human mesenchymal stem cells from six different tissues for their transplantation-relevant potential. Furthermore, for prospective allogenic transplantation we developed a semipermeable hollow-fiber membrane enclosure, which would prevent cell fusion, would provide an immune barrier, and would allow for easy removal of donor cells from patients after recovery. We investigated human mesenchymal stem cells from adipose tissue, amniotic tissue, bone marrow, chorionic tissue, liver, and umbilical cord. We compared their multilineage differentiation potential, secretion of growth factors, and the expression of genes and surface markers. We found that although the expression of typical mesenchymal stem cell-associated gene THY1 and surface markers CD90 and CD73 were mostly similar between mesenchymal stem cells from different donor sites, their expression of lineage-specific genes, secretion of growth factors, multilineage differentiation potential, and other surface markers were considerably different. The encasement of mesenchymal stem cells in fibers affected the various mesenchymal stem cells differently depending on their donor site. Conclusively, mesenchymal stem cells isolated from different tissues were not equal, which should be taken into consideration when deciding for optimal sourcing for therapeutic transplantation. The encasement of mesenchymal stem cells into semipermeable membranes could provide a physical immune barrier, preventing cell fusion.

Mesenchymal stem cells cannot affect mRNA expression of toll-like receptors in different tissues during sepsis

2017 ◽  
Vol 66 (7) ◽  
pp. 547-551 ◽  
Author(s):  
Leonardo Pedrazza ◽  
Talita Carneiro Brandão Pereira ◽  
Ana Lucia Abujamra ◽  
Fernanda Bordignon Nunes ◽  
Maurício Reis Bogo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mrna Expression ◽  
Toll Like Receptors ◽  
Different Tissues

Genomic and Proteomic Signatures of Human Mesenchymal Stem Cells.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 2300-2300
Author(s):  
Wolfgang Wagner ◽  
Robert E. Feldmann ◽  
Frederik Wein ◽  
Anja Seckinger ◽  
Ulf Krause ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture Conditions ◽  
Clinical Applications ◽  
Differentially Expressed ◽  
Cdna Clones ◽  
Reference Map ◽  
Definition Of ◽  
Different Tissues

Abstract Mesenchymal stem cells (MSC) raise high hopes in clinical applications. However, the lack of common standards and a precise definition of MSC preparations remains a major obstacle in research and application of MSC. Whereas surface antigen markers have failed to precisely define this population, a combination of proteomic data and microarray data provides a new dimension for the definition of MSC preparations. To define the molecular signatures at a genomic and proteomic level we have compared the MSC preparations isolated from different sources (adipose tissue, bone marrow and umbilical cord blood) from different donors, as well as from the same donor cultured under different conditions. As a references we have used non-multipotent human fibroblasts (HS68 and NHDF). No phenotypic differences were observed between MSC and fibroblasts by flow cytometry using a panel of 22 surface antigen markers. Gene expression profiles were compared by cDNA microarray analysis (51,144 different cDNA clones of the RZPD3 Unigene Set). Twenty-five genes were overlapping more than two-fold up-regulated in all MSC preparations and these included fibronectin, ECM2, glypican-4, ID1, NF1B, HOXA5 and HOXB6 whereas several inhibitors of the Wnt-pathway (DKK1, DKK3, SFRP1) were higher expressed in fibroblasts. Differential gene expression was verified for 20 genes by RT-PCR. Hierarchical cluster analysis revealed a correlation of four individual donor samples for each MSC preparation while pair wise comparison of MSC from different tissues or culture-isolation procedures revealed between 206 to 1113 differentially expressed ESTs (p<0.001). Using two-dimensional gel electrophoresis and mass spectometry we have generated a proteome reference map of MSC. 136 protein spots were unambiguously identified most of which play a role in cytoskeleton, protein folding and metabolism. This reference map was used to compare protein expression of MSC isolated under different culture conditions. Corresponding cDNA clones were then selected on our microarray. Combination of datasets revealed that genes that were differentially expressed on mRNA level (p<0.05) were differentially expressed on protein level as well (Pearson correlation = 0.83). Interchanging culture conditions revealed that differential expression was directly regulated by medium in some genes whereas it remained constant in others. Expressions of fibronectin, vimentin, myosin, tropomyosin and ezrin were analyzed by fluorescence microscopy whereby no subpopulations could be discriminated within cell preparations while marked differences were observed in morphology and organization of MSC isolated under different culture conditions. Our results provide evidence for reproducible isolation of similar and homogeneous MSC preparations under standardized isolation conditions, while MSC from different tissues or culture conditions display significant differences in their transcriptome, proteome and cellular organization. Our comparative approach provides foundation for a reliable quality control using genotypic and proteomic analysis for clinical applications.

scholarly journals Multiple Functions of MSCA-1/TNAP in Adult Mesenchymal Progenitor/Stromal Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
David Estève ◽  
Jean Galitzky ◽  
Anne Bouloumié ◽  
Caroline Fonta ◽  
René Buchet ◽  
...  
Keyword(s):  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Mesenchymal Stem Cells ◽  
Stromal Cells ◽  
Proof Of Concept ◽  
Wide Range ◽  
Semantic Shift ◽  
Definition Of ◽  
Different Tissues

Our knowledge about mesenchymal stem cells has considerably grown in the last years. Since the proof of concept of the existence of such cells in the 70s by Friedenstein et al., a growing mass of reports were conducted for a better definition of these cells and for the reevaluation from the term “mesenchymal stem cells” to the term “mesenchymal stromal cells (MSCs).” Being more than a semantic shift, concepts behind this new terminology reveal the complexity and the heterogeneity of the cells grouped in MSC family especially as these cells are present in nearly all adult tissues. Recently, mesenchymal stromal cell antigen-1 (MSCA-1)/tissue nonspecific alkaline phosphatase (TNAP) was described as a new cell surface marker of MSCs from different tissues. The alkaline phosphatase activity of this protein could be involved in wide range of MSC features described below from cell differentiation to immunomodulatory properties, as well as occurrence of pathologies. The present review aims to decipher and summarize the role of TNAP in progenitor cells from different tissues focusing preferentially on brain, bone marrow, and adipose tissue.

scholarly journals Comparison of Mesenchymal Stem Cells from Different Tissues to Suppress T-Cell Activation

2007 ◽  
Vol 16 (5) ◽  
pp. 555-562 ◽  
Author(s):  
Kirsten A. Keyser ◽  
Karen E. Beagles ◽  
Hans-Peter Kiem
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Different Tissues
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