scholarly journals Mesenchymal Stem Cells from Different Tissues: Immune Status and Activity

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
Trivanović D ◽  
Krstić J ◽  
Mojsilović S ◽  
Djordjević IO ◽  
Ilić V ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Immune Status ◽  
Different Tissues

Comparing the Chondrogenic Potential in vivo of Autogeneic Mesenchymal Stem Cells Derived from Different Tissues

2010 ◽  
Vol 39 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Qiang Li ◽  
Jicun Tang ◽  
Riying Wang ◽  
Chaoyong Bei ◽  
Linwei Xin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Potential ◽  
Different Tissues

scholarly journals Comparative adhesive and migratory properties of mesenchymal stem cells from different tissues

Biorheology ◽  
2019 ◽  
Vol 56 (1) ◽  
pp. 15-30 ◽  
Author(s):  
Asma Alanazi ◽  
Hafsa Munir ◽  
Mohammed Alassiri ◽  
Lewis S.C. Ward ◽  
Helen M. McGettrick ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Different Tissues

Characteristics of mesenchymal stem cells derived from different tissues of goat

2019 ◽  
Vol 43 (3) ◽  
pp. 107-114
Author(s):  
Na-Yeon Gu ◽  
◽  
Da-Un Jeong ◽  
Jeong Su Byeon ◽  
Jae-Young Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Different Tissues

scholarly journals Comparison of biological features of wild European rabbit mesenchymal stem cells derived from different tissues.

2021 ◽  
Author(s):  
Marta Díaz-de Frutos ◽  
Alexandra Calle ◽  
María Zamora-Ceballos ◽  
Juan Bárcena ◽  
Esther Blanco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
European Rabbit ◽  
Isolation And Characterization ◽  
Expression Characteristic ◽  
Subcutaneous Adipose ◽  
Different Tissues

Although the European rabbit is an "endangered" species and a notorious biological model, the analysis and comparative characterization of new tissue sources of rabbit mesenchymal stem cells (rMSCs) has not been well studied. Here we report for the first time the isolation and characterization of rMSCs derived from an animal belonging to a natural rabbit population within the species native region. New rMSC lines were isolated from different tissues: oral mucosa (rOM-MSC), dermal skin (rDS-MSC), subcutaneous adipose tissue (rSCA-MSC), ovarian adipose tissue (rOA-MSC), oviduct (rO-MSC), and mammary gland (rMG­MSC). The six rMSC lines showed plastic adhesion with fibroblast-like morphology and were all shown to be positive for CD44 and CD29 expression (characteristic markers of MSCs), and negative for CD34 or CD45 expression. In terms of pluripotency features, all rMSC lines expressed NANOG, OCT4, and SOX2. Furthermore, all rMSC lines cultured under osteogenic, chondrogenic, and adipogenic conditions showed differentiation capacity. In conclusion, this study describes the isolation and characterization of new rabbit cell lines from different tissue origins, with a clear mesenchymal pattern. We show that rMSC do not exhibit differences in terms of morphological features, expression of the cell surface, and intracellular markers of pluripotency and in vitro differentiation capacities, attributable to their tissue of origin.

scholarly journals Characterization of Human Mesenchymal Stem Cells from Different Tissues and Their Membrane Encasement for Prospective Transplantation Therapies

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Eva Schmelzer ◽  
Daniel T. McKeel ◽  
Jörg C. Gerlach
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Cell Fusion ◽  
Human Mesenchymal Stem Cells ◽  
Surface Markers ◽  
Multilineage Differentiation ◽  
Differentiation Potential ◽  
Donor Cells ◽  
Different Tissues

Human mesenchymal stem cells can be isolated from various organs and are in studies on therapeutic cell transplantation. Positive clinical outcomes of transplantations have been attributed to both the secretion of cytokines and growth factors as well as the fusion of donor cells with that of the host. We compared human mesenchymal stem cells from six different tissues for their transplantation-relevant potential. Furthermore, for prospective allogenic transplantation we developed a semipermeable hollow-fiber membrane enclosure, which would prevent cell fusion, would provide an immune barrier, and would allow for easy removal of donor cells from patients after recovery. We investigated human mesenchymal stem cells from adipose tissue, amniotic tissue, bone marrow, chorionic tissue, liver, and umbilical cord. We compared their multilineage differentiation potential, secretion of growth factors, and the expression of genes and surface markers. We found that although the expression of typical mesenchymal stem cell-associated gene THY1 and surface markers CD90 and CD73 were mostly similar between mesenchymal stem cells from different donor sites, their expression of lineage-specific genes, secretion of growth factors, multilineage differentiation potential, and other surface markers were considerably different. The encasement of mesenchymal stem cells in fibers affected the various mesenchymal stem cells differently depending on their donor site. Conclusively, mesenchymal stem cells isolated from different tissues were not equal, which should be taken into consideration when deciding for optimal sourcing for therapeutic transplantation. The encasement of mesenchymal stem cells into semipermeable membranes could provide a physical immune barrier, preventing cell fusion.

scholarly journals Bone Marrow-Derived Mesenchymal Stem Cells Exert Diverse Effects on Different Macrophage Subsets

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Bin Chen ◽  
Yanhong Ni ◽  
Jiaying Liu ◽  
Yangheng Zhang ◽  
Fuhua Yan
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Immune Status ◽  
M1 Macrophages ◽  
Cd163 Expression ◽  
M1 Macrophage ◽  
Different Types ◽  
Macrophage Apoptosis ◽  
Macrophage Subsets

Mesenchymal stem cells (MSCs) and their secreted molecules have shown great potential for tissue regeneration and the treatment of inflammation and autoimmune diseases. However, they can also be associated with therapeutic failure or even side effects. Possible causes for this could include the state of the stem cells themselves and the influence of the local microenvironment, wherein macrophages play important roles. As such, we utilized conditioned medium from bone marrow-derived MSCs (MSC-CM) and studied its effect on different macrophage subsets. Effects on macrophage proliferation, apoptosis, polarization, and phagocytosis were determined, and it was discovered that MSC-CM had no significant effect on macrophage proliferation but inhibited M0 macrophage apoptosis and marginally induced M1 macrophage apoptosis. MSC-CM was shown to reduce CD80 expression on the surface of M1 macrophages. Moreover, it promoted and inhibited CD163 expression on the surface of M0 and M1 macrophages, respectively. However, MSC-CM tended to initially promote CD163 expression on M2 macrophages but inhibited expression of this marker after additional incubation time. Unlike MSCs, MSC-CM had no significant effect on the expression of TNF-α and IL-10 in macrophages. Thus, the effect of MSC-CM on different types of macrophages is different, and after stem cells are implanted, their effects on the local immune microenvironment are closely related to the original immune status of the implantation site. Therefore, we suggest that when utilizing stem cells for therapeutics, the immune status of the treatment site should be fully elucidated.

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