VariantSalmonellaGenomic Island 1 Antibiotic Resistance Gene Cluster inSalmonella entericaSerovar Albany

ABSTRACT A new antibiotic resistance gene cluster comprising genes for sulfonamide (sul2), streptomycin (strA-strB), and tetracycline [tetR-tet(H)] resistance was detected on plasmid pVM111 from Pasteurella multocida. The tetR-tet(H) gene region was inserted between sul2 and strA, possibly by illegitimate recombination. Two potential recombination sites of 18 and 25 bp were identified.

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Occurrence of a Salmonella enterica Serovar Typhimurium DT104-Like Antibiotic Resistance Gene Cluster Including thefloR Gene in S. enterica Serovar Agona

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.44.5.1359-1361.2000 ◽

2000 ◽

Vol 44 (5) ◽

pp. 1359-1361 ◽

Cited By ~ 52

Author(s):

Axel Cloeckaert ◽

Karim Sidi Boumedine ◽

Geraldine Flaujac ◽

Hein Imberechts ◽

Inge D'Hooghe ◽

...

Keyword(s):

Antibiotic Resistance ◽

Gene Cluster ◽

Resistance Gene ◽

Salmonella Enterica ◽

Salmonella Enterica Serovar Typhimurium ◽

Antibiotic Resistance Gene ◽

Cross Resistance ◽

Chromosomal Locus ◽

Resistance Gene Cluster ◽

Serovar Typhimurium

ABSTRACT Recently a chromosomal locus possibly specific for Salmonella enterica serovar Typhimurium DT104 has been reported that contains a multiple antibiotic resistance gene cluster. Evidence is provided that Salmonella enterica serovar Agona strains isolated from poultry harbor a similar gene cluster including the newly described floR gene, conferring cross-resistance to chloramphenicol and florfenicol.

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Variant Salmonella Genomic Island 1-L Antibiotic Resistance Gene Cluster in Salmonella enterica Serovar Newport

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00697-06 ◽

2006 ◽

Vol 50 (11) ◽

pp. 3944-3946 ◽

Cited By ~ 21

Author(s):

Axel Cloeckaert ◽

Karine Praud ◽

Benoît Doublet ◽

Marie Demartin ◽

François-Xavier Weill

Keyword(s):

Antibiotic Resistance ◽

Gene Cluster ◽

Resistance Gene ◽

Salmonella Enterica ◽

Genomic Island ◽

Antibiotic Resistance Gene ◽

Gene Cassette ◽

Class 1 Integron ◽

Resistance Gene Cluster ◽

Class 1

ABSTRACT We report a new Salmonella genomic island 1 variant antibiotic resistance gene cluster called SGI1-L in a Salmonella enterica serovar Newport isolate containing a dfrA15 gene cassette conferring resistance to trimethoprim. The isolate carried another class 1 integron containing the aacC5 and aadA7 gene cassettes conferring resistance to gentamicin and streptomycin/spectinomycin, respectively.

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Variant Salmonella Genomic Island 1 Antibiotic Resistance Gene Cluster Containing a Novel 3′-N-Aminoglycoside Acetyltransferase Gene Cassette, aac(3)-Id, in Salmonella enterica Serovar Newport

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.48.10.3806-3812.2004 ◽

2004 ◽

Vol 48 (10) ◽

pp. 3806-3812 ◽

Cited By ~ 64

Author(s):

Benoît Doublet ◽

François-Xavier Weill ◽

Laetitia Fabre ◽

Elisabeth Chaslus-Dancla ◽

Axel Cloeckaert

Keyword(s):

Antibiotic Resistance ◽

Gene Cluster ◽

Resistance Gene ◽

Salmonella Enterica ◽

Genomic Island ◽

Antibiotic Resistance Gene ◽

Gene Cassette ◽

Multidrug Resistant ◽

Resistance Gene Cluster ◽

New Variant

ABSTRACT Salmonella genomic island 1 (SGI1) harbors an antibiotic resistance gene cluster and was previously identified in the multidrug-resistant Salmonella enterica serovars Typhimurium DT104, Agona, Paratyphi B, and Albany. This antibiotic resistance gene cluster is a complex class 1 integron and most often confers resistance to ampicillin (Ap), chloramphenicol (Cm)/florfenicol (Ff), streptomycin (Sm)/spectinomycin (Sp), sulfonamides (Su), and tetracycline (Tc) (ApCmFfSmSpSuTc profile). Recently, variant SGI1 antibiotic resistance gene clusters conferring different antibiotic resistance profiles have been identified in several S. enterica serovars and were classified as SGI1-A to -G. We identified a new variant SGI1 antibiotic resistance gene cluster in two multidrug-resistant S. enterica serovar Newport strains isolated from humans in France. In these strains, the Sm/Sp resistance gene cassette aadA2 inserted at the first attI1 site was replaced by two other aminoglycoside resistance gene cassettes. The first one contains a new resistance gene encoding an AAC(3)-I aminoglycoside 3-N-acetyltransferase that confers resistance to gentamicin (Gm) and sisomicin (Sc). This gene has been named aac(3)-Id. The second one harbors the Sm/Sp resistance gene aadA7. This gene cassette replacement in the SGI1 complex integron of serovar Newport strains constitutes a new variant SGI1 antibiotic resistance gene cluster named SGI1-H. The occurrence of SGI1 in different S. enterica serovars, now including serovar Newport, strengthens the hypothesis of horizontal transfer of SGI1.

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Detection of Salmonella spp. in Retail Raw Food Samples from Vietnam and Characterization of Their Antibiotic Resistance

Applied and Environmental Microbiology ◽

10.1128/aem.00972-07 ◽

2007 ◽

Vol 73 (21) ◽

pp. 6885-6890 ◽

Cited By ~ 84

Author(s):

Thi Thu Hao Van ◽

George Moutafis ◽

Taghrid Istivan ◽

Linh Thuoc Tran ◽

Peter J. Coloe

Keyword(s):

Antibiotic Resistance ◽

Nalidixic Acid ◽

Gene Cluster ◽

Resistance Gene ◽

Antimicrobial Agents ◽

Genomic Island ◽

Antibiotic Resistance Gene ◽

Food Samples ◽

Salmonella Spp

ABSTRACT A study was conducted to examine the levels of Salmonella spp. contamination in raw food samples, including chicken, beef, pork, and shellfish, from Vietnam and to determine their antibiotic resistance characteristics. A total of 180 samples were collected and examined for the presence of Salmonella spp., yielding 91 Salmonella isolates. Sixty-one percent of meat and 18% of shellfish samples were contaminated with Salmonella spp. Susceptibility of all isolates to a variety of antimicrobial agents was tested, and resistance to tetracycline, ampicillin/amoxicillin, nalidixic acid, sulfafurazole, and streptomycin was found in 40.7%, 22.0%, 18.7%, 16.5%, and 14.3% of the isolates, respectively. Resistance to enrofloxacin, trimethoprim, chloramphenicol, kanamycin, and gentamicin was also detected (8.8 to 2.2%). About half (50.5%) of the isolates were resistant to at least one antibiotic, and multiresistant Salmonella isolates, resistant to at least three different classes of antibiotics, were isolated from all food types. One isolate from chicken (serovar Albany) contained a variant of the Salmonella genomic island 1 antibiotic resistance gene cluster. The results show that antibiotic resistance in Salmonella spp. in raw food samples from Vietnam is significant.

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