scholarly journals Variant Salmonella Genomic Island 1-L Antibiotic Resistance Gene Cluster in Salmonella enterica Serovar Newport

2006 ◽  
Vol 50 (11) ◽  
pp. 3944-3946 ◽  
Author(s):  
Axel Cloeckaert ◽  
Karine Praud ◽  
Benoît Doublet ◽  
Marie Demartin ◽  
François-Xavier Weill
Keyword(s):  
Antibiotic Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Antibiotic Resistance Gene ◽  
Gene Cassette ◽  
Class 1 Integron ◽  
Resistance Gene Cluster ◽  
Class 1

ABSTRACT We report a new Salmonella genomic island 1 variant antibiotic resistance gene cluster called SGI1-L in a Salmonella enterica serovar Newport isolate containing a dfrA15 gene cassette conferring resistance to trimethoprim. The isolate carried another class 1 integron containing the aacC5 and aadA7 gene cassettes conferring resistance to gentamicin and streptomycin/spectinomycin, respectively.

scholarly journals Variant Salmonella Genomic Island 1 Antibiotic Resistance Gene Cluster Containing a Novel 3′-N-Aminoglycoside Acetyltransferase Gene Cassette, aac(3)-Id, in Salmonella enterica Serovar Newport

2004 ◽  
Vol 48 (10) ◽  
pp. 3806-3812 ◽  
Author(s):  
Benoît Doublet ◽  
François-Xavier Weill ◽  
Laetitia Fabre ◽  
Elisabeth Chaslus-Dancla ◽  
Axel Cloeckaert
Keyword(s):  
Antibiotic Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Antibiotic Resistance Gene ◽  
Gene Cassette ◽  
Multidrug Resistant ◽  
Resistance Gene Cluster ◽  
New Variant

ABSTRACT Salmonella genomic island 1 (SGI1) harbors an antibiotic resistance gene cluster and was previously identified in the multidrug-resistant Salmonella enterica serovars Typhimurium DT104, Agona, Paratyphi B, and Albany. This antibiotic resistance gene cluster is a complex class 1 integron and most often confers resistance to ampicillin (Ap), chloramphenicol (Cm)/florfenicol (Ff), streptomycin (Sm)/spectinomycin (Sp), sulfonamides (Su), and tetracycline (Tc) (ApCmFfSmSpSuTc profile). Recently, variant SGI1 antibiotic resistance gene clusters conferring different antibiotic resistance profiles have been identified in several S. enterica serovars and were classified as SGI1-A to -G. We identified a new variant SGI1 antibiotic resistance gene cluster in two multidrug-resistant S. enterica serovar Newport strains isolated from humans in France. In these strains, the Sm/Sp resistance gene cassette aadA2 inserted at the first attI1 site was replaced by two other aminoglycoside resistance gene cassettes. The first one contains a new resistance gene encoding an AAC(3)-I aminoglycoside 3-N-acetyltransferase that confers resistance to gentamicin (Gm) and sisomicin (Sc). This gene has been named aac(3)-Id. The second one harbors the Sm/Sp resistance gene aadA7. This gene cassette replacement in the SGI1 complex integron of serovar Newport strains constitutes a new variant SGI1 antibiotic resistance gene cluster named SGI1-H. The occurrence of SGI1 in different S. enterica serovars, now including serovar Newport, strengthens the hypothesis of horizontal transfer of SGI1.

scholarly journals Occurrence of a Salmonella enterica Serovar Typhimurium DT104-Like Antibiotic Resistance Gene Cluster Including thefloR Gene in S. enterica Serovar Agona

2000 ◽  
Vol 44 (5) ◽  
pp. 1359-1361 ◽  
Author(s):  
Axel Cloeckaert ◽  
Karim Sidi Boumedine ◽  
Geraldine Flaujac ◽  
Hein Imberechts ◽  
Inge D'Hooghe ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Antibiotic Resistance Gene ◽  
Cross Resistance ◽  
Chromosomal Locus ◽  
Resistance Gene Cluster ◽  
Serovar Typhimurium

ABSTRACT Recently a chromosomal locus possibly specific for Salmonella enterica serovar Typhimurium DT104 has been reported that contains a multiple antibiotic resistance gene cluster. Evidence is provided that Salmonella enterica serovar Agona strains isolated from poultry harbor a similar gene cluster including the newly described floR gene, conferring cross-resistance to chloramphenicol and florfenicol.

Antibiotic resistance profile of Salmonella enterica isolated from exotic and indigenous leafy green vegetables in Accra, Ghana

2021 ◽  
Author(s):  
Jinru Chen ◽  
Joycelyn Quansah
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Gene Cassette ◽  
Nucleotide Sequencing ◽  
Dilution Method ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Class 1

Fresh produce-borne enteric bacterial pathogens with resistance to antibiotics have posed serious challenges to food safety and public health worldwide.  This study examined the antibiotic resistance profile of Salmonella enterica (n=33), previously isolated from exotic and indigenous leafy green vegetable samples (n=328) collected from 50 vegetable farms in 12 farming areas and 37 vegetable sellers in 4 market centers in Accra, Ghana during the period of March 2016 to March 2017, and determined the distribution of integrons among antibiotic-resistant isolates.  The susceptibility of the Salmonella isolates to 12 antibiotics was assayed using the standard disc diffusion assay.  The minimum inhibitory concentrations (MICs) of the five most resisted antibiotics were determined using the twofold macro dilution method.  PCR assay was used to detect the presence of integrons in Salmonella cells, and PCR product with amplified integron gene cassette was purified and sequenced using the Sanger sequencing technology.  The Salmonella isolates used in the study resisted at least one tested antibiotic, and multi-drug resistant (MDR) isolates were 30.3% (10/33).  Most isolates (81.8%) were resistant to sulfisoxazole.  The MICs of tetracycline, cefoxitin, streptomycin, ampicillin, and sulfisoxazole were 16, 32, 64, 64, and > 1,024 µg/ml, respectively.  A total of five different patterns of MDR were observed among the Salmonella isolates, and the common MDR patterns were AAuFox (30.3%) and AAuFoxSSu (18.1%).  One out of the 33 (3.0%) Salmonella isolates tested positive for class 1 integron with a gene cassette of about 800 bp.  Nucleotide sequencing revealed the class 1 integron carried a single gene dfrA7 .  Future studies are needed to confirm whether the consumption of contaminated leafy green vegetables is a route of acquiring antibiotic-resistant Salmonella by consumers in Accra, Ghana.

scholarly journals Detection of Salmonella spp. in Retail Raw Food Samples from Vietnam and Characterization of Their Antibiotic Resistance

2007 ◽  
Vol 73 (21) ◽  
pp. 6885-6890 ◽  
Author(s):  
Thi Thu Hao Van ◽  
George Moutafis ◽  
Taghrid Istivan ◽  
Linh Thuoc Tran ◽  
Peter J. Coloe
Keyword(s):  
Antibiotic Resistance ◽  
Nalidixic Acid ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Antimicrobial Agents ◽  
Genomic Island ◽  
Antibiotic Resistance Gene ◽  
Food Samples ◽  
Salmonella Spp

ABSTRACT A study was conducted to examine the levels of Salmonella spp. contamination in raw food samples, including chicken, beef, pork, and shellfish, from Vietnam and to determine their antibiotic resistance characteristics. A total of 180 samples were collected and examined for the presence of Salmonella spp., yielding 91 Salmonella isolates. Sixty-one percent of meat and 18% of shellfish samples were contaminated with Salmonella spp. Susceptibility of all isolates to a variety of antimicrobial agents was tested, and resistance to tetracycline, ampicillin/amoxicillin, nalidixic acid, sulfafurazole, and streptomycin was found in 40.7%, 22.0%, 18.7%, 16.5%, and 14.3% of the isolates, respectively. Resistance to enrofloxacin, trimethoprim, chloramphenicol, kanamycin, and gentamicin was also detected (8.8 to 2.2%). About half (50.5%) of the isolates were resistant to at least one antibiotic, and multiresistant Salmonella isolates, resistant to at least three different classes of antibiotics, were isolated from all food types. One isolate from chicken (serovar Albany) contained a variant of the Salmonella genomic island 1 antibiotic resistance gene cluster. The results show that antibiotic resistance in Salmonella spp. in raw food samples from Vietnam is significant.

scholarly journals New Plasmid-Borne Antibiotic Resistance Gene Cluster in Pasteurella multocida

2003 ◽  
Vol 47 (9) ◽  
pp. 2978-2980 ◽  
Author(s):  
Corinna Kehrenberg ◽  
Nga Thi Thu Tham ◽  
Stefan Schwarz
Keyword(s):  
Antibiotic Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Pasteurella Multocida ◽  
Antibiotic Resistance Gene ◽  
Illegitimate Recombination ◽  
Gene Region ◽  
Resistance Gene Cluster ◽  
H Gene

ABSTRACT A new antibiotic resistance gene cluster comprising genes for sulfonamide (sul2), streptomycin (strA-strB), and tetracycline [tetR-tet(H)] resistance was detected on plasmid pVM111 from Pasteurella multocida. The tetR-tet(H) gene region was inserted between sul2 and strA, possibly by illegitimate recombination. Two potential recombination sites of 18 and 25 bp were identified.

scholarly journals VariantSalmonellaGenomic Island 1 Antibiotic Resistance Gene Cluster inSalmonella entericaSerovar Albany

2003 ◽  
Vol 9 (5) ◽  
pp. 585-591 ◽  
Author(s):  
Benoît Doublet ◽  
Renaud Lailler ◽  
Danièle Meunier ◽  
Anne Brisabois ◽  
David Boyd ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Antibiotic Resistance Gene ◽  
Resistance Gene Cluster

scholarly journals Salmonella Genomic Island 1 Multidrug Resistance Gene Clusters in Salmonella enterica Serovar Agona Isolated in Belgium in 1992 to 2002

2004 ◽  
Vol 48 (7) ◽  
pp. 2510-2517 ◽  
Author(s):  
Benoît Doublet ◽  
Patrick Butaye ◽  
Hein Imberechts ◽  
David Boyd ◽  
Michael R. Mulvey ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Resistance Gene ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Gene Clusters ◽  
Complex Class ◽  
Class 1 Integron ◽  
Transposase Gene ◽  
New Variant ◽  
Class 1

ABSTRACT Salmonella genomic island 1 (SGI1) harbors a multidrug resistance (MDR) gene cluster which is a complex class 1 integron. Variant SGI1 MDR gene clusters conferring different MDR profiles have also been identified in several Salmonella enterica serovars and classified as SGI1-A to -F. A retrospective study was undertaken to characterize MDR regions from serovar Agona strains harboring SGI1 isolated from poultry in Belgium between 1992 and 2002. A total of 171 serovar Agona strains, displaying resistance to at least one antibiotic, were studied for the presence of SGI1. SGI1 was detected in 94 serovar Agona strains. The most prevalent variant was SGI1-A (85%), which harbors within the SGI1 complex class 1 integron a common region (CR1) containing orf513, a putative transposase gene, adjacent to the dfrA10 trimethoprim resistance gene. A new variant SGI1 named SGI1-G was identified in two strains. It consisted of the pse-1 gene cassette, as in SGI1-B, but with additional insertion of the orf513/dfrA10 region structure. Seven strains displaying the typical SGI1 MDR profile (Ap Cm Ff Sm Sp Su Tc) showed genetic variation at the 3′ end of SGI1. These strains harbored the insertion of the CR1 containing orf513 as in SGI1-A, -D, and -G. However, downstream the right end of CR1, they presented different 7.4- to 8.5-kb deletions of the SGI1 3′ end that extended to the chromosomal genes yieE and yieF. These results suggest a possible role of CR1 in deletion formation, as has been reported for some insertion sequences. Pulsed-field gel electrophoresis analysis showed that all the serovar Agona SGI1-carrying strains belonged to a single clone. Thus, SGI1 is largely encountered in serovar Agona strains isolated from poultry in Belgium, the most prevalent variant being SGI1-A. SGI1 MDR region undergoes recombinational events resulting in a diversity of MDR gene clusters.

Sign in / Sign up

Export Citation Format

Share Document