scholarly journals Isolasi dan Aplikasi Fungi Entomopatogen dari Larva Nyamuk Aedes aegypti L.

2019 ◽  
Vol 11 (2) ◽  
pp. 87-93
Author(s):  
Wuri Artikasari ◽  
Emantis Rosa ◽  
Bambang Irawan
Keyword(s):  
Aedes Aegypti ◽  
Entomopathogenic Fungi ◽  
Negative Impact ◽  
Hemorrhagic Fever ◽  
Mosquito Larvae ◽  
Chemical Insecticide ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Difference Test

DHF (Dengue Hemorrhagic Fever) is a serious problem in Indonesia. DHF disease control has been applied so far, one of which is the use of larvacide temephos (abate). However larvacide is a chemical insecticide that has a negative impact on human health and causes resistance. Therefore in this research, biological control is carried out by utilizing entomopathogenic fungi as a larvacide. The purpose of this study was to determine the effectiveness of entomopathogenic fungi isolated from Ae aegypti larvae. Against the death of Ae. Aegypti mosquito larvae with the moist chamber method. This study used a completely randomized design (CRD) with a factorial pattern and performed two repetitions. Factor A is a type of fungi with 3 levels, namely A1: Aspergillus sp1, A2: Aspergillus sp2, and A3: Syncephalastrum sp. Factor B is a dilution with 7 levels, namely B0: Control, B1: 100 (without dilution), B2: 10-1, B3: 10-2, B4: 10-3, B5: 10-4, B6: 10-5 with every treatment was applied in 2 repetitions. Observations were made 24 hours after treatment for 3 days. Data were analyzed using analysis of variances (anova) and continued with the Least Significant Difference Test (LSD) at 5%. The results indicate that fungi isolates are the most effective in killing Ae. Aegypti mosquito larvae is Aspergillus sp1 and Aspergillus sp2 on the treatment of spores without dilution. Key words: DHF; larvacide; Aedes aegypti; Entomopathogenic Fungi.

scholarly journals EFEKTIVITAS ABATE DAN EKSTRAK DAUN SIRIH (Piper betle) DALAM MEMATIKAN LARVA Aedes Aegypti L INSTAR III

Masker Medika ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 226-232
Author(s):  
Triana Oktaviani ◽  
Zairinayati Zairinayati
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Hemorrhagic Fever ◽  
Piper Betle ◽  
Mosquito Larvae ◽  
Betel Leaf ◽  
Preventive Effort ◽  
Randomized Design ◽  
Natural Insecticides ◽  
Completely Randomized Design

Latar Belakang: Aedes aegypti merupakan nyamuk yang dapat berperan sebagai vektor Demam Berdarah Dengue (DBD). Upaya promotif dan preventif menjadi mutlak dilakukan untuk memutus mata rantai penularan dengan membunuh larva nyamuk dengan larvasida alami menggunakan ekstrak daun sirih (Piper betle) sebagai insektisida alami. Tujuan dari penelitian: ini adalah untuk mengetahui perbedaan abate dan ekstrak daun sirih (Piper betle) dalam mematikan larva Aedes aegypti L instar III. Desain penelitian: ini bersifat eksperimen dengan menggunakan rancangan acak lengkap (RAL) yang terdiri dari 3 perlakuan 1 kontrol dan 8 kali pengulangan percobaan. Hasil Penelitian: dapat diketahui bahwa untuk penggunaan ekstrak daun sirih (Piper betle) dengan konsentrasi 5% dapat mematikan larva sebanyak 8 ekor, sedangkan konsentrasi 10% tidak dapat mematikan larva (0 larva) dan konsentrasi 15% dapat mematikan larva sebanyak 17 (85%) sedangkan untuk penggunaan abate dengan konsentrasi 5%, 10% dan 15% dapat mematikan larva sebanyak 160 larva (100%). Kesimpulan: bahwa abate mempunyai efektifitas lebih tinggi di banding dengan ekstrak daun sirh (Piper betle).     Background: Aedes aegypti is a mosquito that can act as a vector of dengue Hemorrhagic Fever (DHF). Promotove and preventive effort are absolutely necessary to break the chain to transmission by killing mosquito larvae with natural larvacides using betel leaf extract (Piper betle) as a natural insecticides. The purposes of this study: this was to determine the differences in abate and betel leaf extract (Piper batle) in kliing Aedes aegypti instar III larvae. Research methods this is an experimental study using a completely randomized design (CRD) consisting of 3 treatments 1 control and 8 repetitions of the experiment. The results: it can be seen that for the use of betel leaf extract(Piper betle) with a concentration cannot kill larvae in 17 (85%) whilke for the use of abate with a concentration of 5%, 10% and 15% can kill larvae of 160 (100%). Conclusion: that the abate has a higher effectiveness compared to sirih leaf extract.

scholarly journals UJI PENGGUNAAN BERBAGAI MEDIA TUMBUH DAN PEMBERIAN PUPUK GROWMORE PADA AKLIMATISASI TANAMAN ANGGREK Dendrobium

2019 ◽  
Vol 15 (2) ◽  
pp. 105-114
Author(s):  
GUSTI MARLINA ◽  
MARLINDA MARLINDA ◽  
HENI ROSNETI
Keyword(s):  
Plant Height ◽  
Growth Media ◽  
Growing Media ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Honestly Significant Difference Test

The research objective was determine the effect of various growth media and growmore fertilizer on the acclimatization of Dendrobium orchids. study used a Completely Randomized Design (CRD) Factorial consisting of 2 factors, the first is (M) of growing media which consists of 3 levels, namely: M1 (medium fern root), M2 (wood charcoal medium), M3 (coir media coconut) and the second is (G) growmore fertilizer which consists of 4 levels, namely: G0 (without Growmore fertilizer), G1 (Growmore fertilizer 2 g / liter), G2 (fertilizer Growmore 4 g / liter), G3 (Growmore fertilizer 6 g / liter). Determine the growth of orchid plants, observations were made with the following parameters Growth Percentage (%), Plant Height (cm), Number of leaves (strands), and Number of tillers (stems). Data obtained were analyzed statistically, then continued with the Honestly Significant Difference Test (BNJ) at the level of 5%. The results showed that the single treatment of the use of growing media did not have a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). The growth of growmore fertilizer also did not show a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). Interaction of the use of various growth media and growmore fertilizer did not show a significant effect on the percentage of growth (%), plant height (cm), number of leaves (strands), and number of tillers (stems).

scholarly journals EFEKTIFITAS CAMPURAN RENDAMAN JERAMI (ORYZA SATIVA L) DAN TEMEFOS SEBAGAI ATRAKTAN TERHADAP LETHAL OVITRAP AEDES AEGYPTI L

2020 ◽  
Vol 19 (2) ◽  
pp. 112-118
Author(s):  
Muhammad Rasyid Ridha ◽  
Budi Hairani ◽  
Gusti Meliyanie ◽  
Wulan Rasna Giri Sembiring ◽  
Abdullah Fadilly ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Control Strategy ◽  
Oryza Sativa L ◽  
Larval Mortality ◽  
Hemorrhagic Fever ◽  
Alternative Control ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Global Health Problem

ABSTRACT Dengue hemorrhagic fever is a global health problem and can be transmitted through vectors, namely Aedes aegypti. One of the controls can be through lethal ovitrap combined with attractant. This study aims to compare the percentage of trapped eggs, the number of eggs hatched, and larval mortality in the lethal ovitrap that were given two additional types of attractants. The insecticides used were temephos, while the attractants used were 20% straw soaking water, and water that Ae. aegypti have used to lay eggs. The type of research is an experiment with a completely randomized design. The sample used was female mosquito of Ae. aegypti that is full of blood from laboratory colonization. The results showed that straw soaking water was more influential attractant than the former Ae. aegypti colonization water in attracting Ae. aegypti mosquitoes to lay eggs. The highest larval mortality was found in a combination of lethal ovitrap with straw soaking water. Statistically there is an influence of the type of attractant on hatchability and the development of Ae. aegypti into adult mosquitoes. The combination of lethal ovitrap and attractant of straw soaking water can be an alternative control strategy for DHF program managers to reduce the density of Ae. aegypti mosquitoes and minimize transmission of dengue hemorrhagic fever in an area. Keywords: Aedes aegypti, attractant, lethal ovitrap, straw soaking water   ABSTRAK Demam berdarah dengue merupakan masalah kesehatan global dan dapat ditularkan melalui vektor yaitu Aedes aegypti. Salah satu pengendaliannya dapat melalui lethal ovitrap yang dipadukan dengan atraktan. Penelitian ini bertujuan untuk membandingkan persentase jumlah telur terperangkap, jumlah telur menetas, dan mortalitas larva pada lethal ovitrap yang diberi tambahan dua jenis atraktan. Insektisida yang digunakan adalah temefos, sedangkan atraktan yang digunakan adalah air rendaman jerami dengan konsentrasi 20%, dan air bekas kolonisasi/telur larva Ae. aegypti. Jenis penelitian ini adalah eksperimen dengan rancangan acak lengkap. Sampel yang digunakan adalah nyamuk Ae. aegypti betina yang kenyang darah hasil kolonisasi di laboratorium. Hasil penelitian menunjukkan bahwa air rendaman jerami merupakan atraktan yang lebih berpengaruh dibandingkan air bekas kolonisasi Ae. aegypti dalam menarik nyamuk Ae. aegypti untuk bertelur. Mortalitas larva tertinggi terdapat pada kombinasi lethal ovitrap dengan air rendaman jerami. Secara statistik ada pengaruh jenis atraktan terhadap daya tetas dan perkembangan Ae. aegypti menjadi nyamuk dewasa. Kombinasi lethal ovitrap dengan atraktan air rendaman jerami dapat menjadi strategi pengendalian alternatif bagi pengelola program DBD untuk mengurangi kepadatan nyamuk Ae. aegypti dan meminimalisasi transmisi penyakit demam berdarah dengue di suatu wilayah. Kata kunci: Aedes aegypti, atraktan, lethal ovitrap, rendaman jerami

scholarly journals The Toxicity of Srikaya Seed Granules (Annona squamosa L.) with Different Heating Temperatures Against the Larva of Aedes aegypti L.

BIOEDUKASI ◽  
2020 ◽  
pp. 1
Author(s):  
Rima Gloria Purwanto ◽  
Dwi Wahyuni ◽  
Joko Waluyo
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Heating Temperature ◽  
Probit Analysis ◽  
Mosquito Larvae ◽  
Annona Squamosa ◽  
Forest Region ◽  
Randomized Design ◽  
Significant Difference ◽  
Lower Temperature

Abstract: Aedes aegypti L. is a mosquito carrying dengue virus that causes dengue fever, especially in Southeast Asia which is a tropical rain forest region which is a habitat for mosquito growth. Aedes aegypti L. mosquito control is carried out by chemical means of fogging and using abate but this control actually causes Aedes aegypti L. mosquitoes to become resistant or immune to chemical drugs so that a new breakthrough is created by making bioinsecticides biologically to eradicate the Aedes aegypti L. mosquito. with characteristics that are environmentally friendly and do not kill non-target animals and cause the Aedes aegypti L. mosquito not to become resistant. To be able to realize this desire so that the sugar cane granules extract of Annona squamosa L. containing active compounds in the form of annonain and squamosin so that they are toxic to Aedes aegypti L. mosquito larvae. Further research is to test the heating temperature level of the granules of Annona squamosa L. extract temperature of 40 ° C and 60 ° C which is more deadly of Aedes aegypti L. mosquito larvae. This research method uses a completely randomized design with four repetitions. The temperature of 60 ° C uses concentrations of 1 ppm, 6 ppm, 12 ppm, 18 ppm, 24 ppm and 30 ppm. As for the temperature of 40 ° C using concentrations of 1 ppm, 7 ppm, 14 ppm, 21 ppm, 28 ppm, and 35 ppm, each temperature compared with aquadest and abate. Data analyzed using probit analysis to determine the LC50, then followed by a statistical test paired sample T-test with SPSS to find out a significant difference between heating temperatures of 40 ° C to 60 ° C. These results then show that a higher temperature of 60 ° C has a higher level of toxicity compared to a lower temperature of 40 ° C.

scholarly journals Giving Lemna Minor and Mud to the Growth of Tubifex sp.

2019 ◽  
Vol 1 (3) ◽  
pp. 23-27
Author(s):  
Mahendra Mahendra ◽  
Doni Damara ◽  
Mursidatun Nufus ◽  
Vira Riana Putri
Keyword(s):  
Analysis Of Variance ◽  
Lemna Minor ◽  
Research Results ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Difference Test

This study aims to determine the effect of the use of a combination of lemna minor substrates and mud on the biomass of the population of Tubifex sp. This research is experimental, with a completely randomized design (CRD), with five levels of treatment P1 = 100% (lemna minor ) + 2,000 gr (mud), P2 = 75% (lemna minor ) + 2,000 gr (mud), P3 = 50 % (lemna minor ) + 2,000 gr (mud), P4 = 25% (lemna minor) + 2,000 gr (mud), P5 = 0% (lemna minor) + 2,000 gr (mud). Data obtained from research results in analysis of variance (ANOVA) and in further testing with the Smallest Significant Difference test. The main parameters observed were the number of population and biomass of Tubifex sp. The results of the study showed that the combination of Lemna minor substrate and mud significantly (p <0.05) on the growth of biomass and the population of Tubifex sp. The best results were found in the treatment with the treatment formulation P1 = 100% (lemna minor ) + 2,000 gr (mud) which produced a population of 6,250 ind / gr and biomass 8.60 gr / m2.

scholarly journals Performance of perisel as shelter and periphyton substrate in the floating cage of vanname shrimp culture

2021 ◽  
Vol 20 (1) ◽  
pp. 65-71
Author(s):  
Rifka Liling Palinggi ◽  
Tatag Budiardi ◽  
Daniel Djokosetiyanto
Keyword(s):  
Negative Impact ◽  
Initial Density ◽  
Shrimp Farming ◽  
Production Performance ◽  
Feed Conversion ◽  
Shrimp Culture ◽  
Randomized Design ◽  
Significant Difference ◽  
Feed Source ◽  
Completely Randomized Design

Shrimp culture in the floating cage is expected to reduce the utilization of land and its possible negative impact to the environment. The advantages of shrimp farming in the sea include the high dissolved oxygen concentration and the better meat quality. This research aimed to enhance the production performance of shrimp through the utilization of periphyton as a natural feed for shrimp. A completely randomized design with 3 treatments (in triplicates) were applied in this experiment, i.e floating cage without perishel (control), floating cage with PE perishel and PA perishel. Shrimp with body weight of 2.5 ± 0.2 g were stocked at the initial density of 2,000 shrimp in each cage, and maintained for 90 days. No significant difference was observed in the survival amongst treatments. The lowest feed conversion ratio (1.74) and coefficient of variance (3.21) were showed in treatment PA. The highest attachment and abundances of periphyton were found in treatment PA. It was concluded that the addition of perishel inside the floating cage may contribute as natural feed source for the shrimp and thus increase the production performance of shrimp. Keywords: floating cage, Litopenaeus vannamei, periphyton, perisel, shelter ABSTRAK Budidaya udang di KJA diharapkan dapat menekan isu pemanfaatan daratan sebagai tambak yang berdampak pada permasalahan lingkungan. Keunggulan laut untuk budidaya udang, antara lain adalah kadar oksigen terlarut relatif tinggi sehingga tidak perlu kincir, dan mutu daging udang yang dihasilkan relatif lebih baik. Penelitian ini ditujukan untuk meningkatkan kinerja produksi dan memanfaatkan kesuburan perairan laut berupa perifiton sebagai pakan alami bagi udang. Penelitian ini terdiri atas tiga perlakuan, yakni: (A) kontrol (tanpa perishel), (B) jaring benang nilon (PE), dan (C) jaring benang serabut pendek (PA 6.6), masing-masing perlakuan terdiri dari tiga ulangan. Udang dengan bobot 2,5 ± 0,2 g ditebar sebanyak 2.000 ekor perwadah, dan dipelihara selama 90 hari. Hasil analisis kinerja produksi menunjukkan bahwa kelangsungan hidup tidak berbeda nyata antarperlakuan. Nilai konversi pakan terendah diperoleh pada perlakuan jaring benang PA dengan nilai 1,74. Nilai koefisien keragaman terendah diperoleh pada perlakuan jaring benang PA dengan nilai 3,21. Penempelan dan kepadatan perifiton yang cukup baik diperoleh pada jenis perishel jaring benang serabut pendek PA. Dengan demikian dapat disimpulkan bahwa dengan penambahan perishel sebagai shelter dan penumbuh perifiton diperoleh hasil produksi yang baik. Udang dapat memanfaatkan keberadaan perishel dalam wadah pemeliharaan sebagai tempat berlindung dan memperoleh makanan tambahan berupa pakan alami yang menempel pada perishel. Kata kunci: karamba jaring apung, perifiton, perisel, selter, udang vaname

scholarly journals PENGARUH PROPORSI DAMI NANGKA TERHADAP KARAKTERISTIK KIMIA, FISIK DAN ORGANOLEPTIK SELAI LEMBARAN NANAS

Pro Food ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 685
Author(s):  
Ni Made Neni Parmiutari ◽  
Eko Basuki ◽  
Rucitra Widyasari
Keyword(s):  
Moisture Content ◽  
Analysis Of Variance ◽  
Chemical Properties ◽  
Randomized Design ◽  
Yellow Color ◽  
Significant Difference ◽  
L Value ◽  
Completely Randomized Design ◽  
Total Dissolved Solid ◽  
Difference Test

ABSTRACT             This study aims to determine the chemical properties (moisture content, pH and total dissolved solid), physical properties (texture and color) and organoleptics (taste, color, aroma, texture) in the different formulation of slice jam from mixture straw jackfruit and pineapple. This study used a Completely Randomized Design (CRD) with 6 treatments and 3 time replications and analyzed using Analysis of Variance at the 5% level and followed by Least Significant Difference test at the 5% level. These treatments include P0 (100 % pineapple), P1 (80% pineapple: 20% straw jackfruit), P2 (65% pineapple: 35% straw jackfruit), P3 (50% pineapple: 50% straw jackfruit), P4 (35% pineapple: 65% straw jackfruit), dan P5 (20% pineapple: 80% straw jackfruit). Observations made on moisture content, pH, total dissolved solid, texture, color and organoleptic taste, color, texture and aroma (scoring and hedonic). The results showed that the P3 treatment (50% straw jackfruit: 50% pineapple) was the preferred treatment for the panelists on the parameters of color, taste, and texture with the characteristics of moisture content 23.35%, pH 4.86, total dissolved solids 32,46oBrix, L value 44.79 and Hue value 70.67o and brownish yellow color, slightly pineapple taste, chewy texture and pineapple aroma and slightly jackfruit aroma. Keywords: pineapple, slice jam, straw jackfruit ABSTRAK       Penelitian ini bertujuan untuk mengetahui sifat kimia (kadar air, pH dan total padatan terlarut), sifat fisik (tekstur dan warna) dan organoleptik (rasa, warna, aroma, tekstur) pada formulasi selai lembaran dari campuran dami nangka dan buah nanas yang berbeda. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 3 kali ulangan dan dianalisis menggunakan analisis keragaman (Analysis of Variance) pada taraf 5% dan dilanjutkan dengan uji Beda Nyata Terkecil pada taraf 5%. Perlakuan diantaranya P0 (100 % buah nanas), P1 (80% buah nanas: 20% dami nangka), P2 (65% buah nanas: 35% dami nangka), P3 (50% buah nanas: 50% dami nangka), P4 (35% buah nanas: 65% dami nangka), dan P5 (20% buah nanas: 80% dami nangka). Pengamatan dilakukan terhadap kadar air, pH, total padatan terlarut, tekstur, warna dan organoleptik rasa, warna, tekstur dan aroma (skoring dan hedonik). Hasil penelitian menunjukkan bahwa perlakuan P3 (50% dami nangka : 50% buah nanas) merupakan perlakuan yang agak disukai panelis pada parameter warna, rasa, dan tekstur dengan karakteristik kadar air 23,35%, pH 4,86, total padatan terlarut 32,46oBrix, nilai L 44,79 dan nilai Hue 70,67o serta warna kuning kecokelatan, agak berasa asam nanas, tekstur kenyal dan beraroma nanas dan sedikit beraroma nangka. Kata kunci: buah nanas, dami nangka, selai lembaran

scholarly journals Aplikasi IJEN (Infeksi Jamur Entomopatogen pada Nyamuk) : Jamur Metarhizium anisopliae pada Nyamuk Aedes aegypti

2019 ◽  
Vol 13 (1) ◽  
pp. 33-48
Author(s):  
Liestiana Indriyati ◽  
Salamiah Salamiah ◽  
Lutfhi Fatah ◽  
Eko Suhartono ◽  
Muhammad Rasyid Ridha ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Olive Oil ◽  
Experimental Research ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Fungal Growth ◽  
Hemorrhagic Fever ◽  
Small Risk ◽  
Randomized Design ◽  
Eggs And Larvae

Abstract Dengue hemorrhagic fever is one of the main problems in Indonesia, its handling on chemical insecticides with insecticide resistance constraints that can inhibit vector control efforts. Entomopathogenic fungi especially Metarhizium anisopliae is one of bioinsecticides that has been widely used in agriculture for controlling insect attacks, effective in killing mosquitoes, safe for vertebrates, humans and the environment and has a small risk of resistance. Appropriate methods of formulation and application for M. anisopliae infection to mosquitoes suitable for mosquito bionomic and fungus characteristics are required. Experimental research with complete randomized design was conducted to test the efficacy of.  M.anisopliae solution mixed olive oil and honey formula applied to ovitrap, plant trap and black cotton trap in infecting adult female Ae. aegypti. The death of Ae.a egypti and fungal growth on kadaver was observed everyday. 100% Ae. aegypti death was obtained in the ovitrap method with mixed formulation of M.anisopliae and olive oil. This method was also capable of infecting Ae.aegypti in various phases of both adults, eggs and larvae so that in this study ovitrap olive oil method was the most effective method for infecting M.anisopliae on Ae.aegypti. Abstrak Demam berdarah dengue merupakan salah satu masalah utama di Indoneisa. Penggunaan insektisida kimia sebagai upaya pengendalian DBD saat ini memiliki kendala resistensi insektisida yang dapat menghambat upaya pengendalian vektor. Jamur entomopatogen khususnya Metarhizium anisopliae adalah salah satu bioinsektisida yang telah digunakan secara luas di bidang pertanian untuk pengendalian serangan serangga efektif membunuh nyamuk, aman bagi vertebrata, manusia dan lingkungan serta memiliki risiko resistensi yang kecil. Diperlukan metode yang tepat baik dari formulasi maupun cara aplikasi untuk penginfeksian M.anisopliae kepada nyamuk yang sesuai dengan bionomik nyamuk dan karakteristik jamur. Penelitian eksperimental dengan rancangan acak lengkap dilakukan untuk menguji efektivitas formula M.anisopliae. Larutan  M.anisopliae dicampur minyak zaitun dan madu yang diaplikasikan pada ovitrap, plant trap dan black cotton trap dalam menginfeksi Ae.aegypti betina dewasa. Pengamatan dilakukan pada  kematian Ae.aegypti dan pertumbuhan jamur pada kadaver setiap hari. Kematian Ae.aegypti 100% didapatkan pada metode ovitrap dengan formulasi campuran M.anisopliae dan minyak zaitun. Metode ini juga mampu menginfeksi Ae.aegypti pada berbagai fase baik dewasa, telur maupun larva sehingga pada penelitian ini metode zaitun ovitrap dinyatakan metode yang paling efektif untuk penginfeksian M.anisopliae pada Ae.aegypti.

scholarly journals Pengaruh Waktu Pengomposan Media Tanam terhadap Pertumbuhan dan Hasil Jamur Tiram Putih (Pleorotus ostreatus)

2021 ◽  
Vol 8 (1) ◽  
pp. 19-28
Author(s):  
Heri Kusnayadi ◽  
Indra Wira Pratama ◽  
Ikhlas Suhada ◽  
Nila Wijayanti
Keyword(s):  
Vegetable Protein ◽  
Fruit Weight ◽  
Growth And Yield ◽  
Emergence Time ◽  
Reduce Body Weight ◽  
Randomized Design ◽  
Significant Difference ◽  
Oyster Mushrooms ◽  
Completely Randomized Design ◽  
Difference Test

The cultivation of consumption mushrooms in Indonesia is showing an encouraging development. Currently, Indonesia is included as one of the world's major mushroom suppliers. Mushrooms are a source of vegetable protein that does not contain cholesterol and can prevent high blood pressure, heart disease, diabetes, and can reduce body weight. The research objective was to determine the effect of composting time on planting media on the growth and yield of white oyster mushrooms. The research was conducted in the Mapin Kebak Village, sub-district Alas Barat star from May to June 2020. The research was conducted using an experimental method and using a completely randomized design consisting of 5 treatments, where each treatment consisted of three replications. The research treatments consisted of M1 = composting for 6 hours; M2 = composting for 12 hours; M3 = composting for 24 hours; M4 = composting for 36 hours and M5 = composting for 48 hours. Furthermore, the data from the observations were analyzed by ANOVA at the real level of 5%. If the results of the data analysis show a significant difference, then a further test is carried out using the Least Significant Difference Test at the 5% real level. The results of the analysis showed that the fastest time to spread mycelium was in the M5 treatment, the composting time was 48 hours. The fastest primordia emergence time was 24 hours composting M3 treatment. The heaviest fruit weight was in treatment M3 and the lowest fruit weight was in treatment M1.

scholarly journals Isolasi dan Uji Patogenitas Isolat Fungi Entomopatogen Terhadap Stadium Dewasa Nyamuk Aedes aegypti

2019 ◽  
Vol 11 (1) ◽  
pp. 33-41
Author(s):  
Supiyanto Supiyanto ◽  
Emantis Rosa ◽  
Bambang Irawan ◽  
Nismah Nukmal
Keyword(s):  
Aedes Aegypti ◽  
Key Words ◽  
Entomopathogenic Fungi ◽  
Microbiology Laboratory ◽  
Penicillium Sp ◽  
Randomized Design ◽  
Mosquito Mortality ◽  
Completely Randomized Design ◽  
Aspergillus Sp

This research aims to determine the effectiveness of four types of entomopathogenic fungi isolated from Ae. aegypti mosquitoes from Bandar Lampung to the mortality of adult stages of Ae. aegypti mosquitoes. This research was conducted in October 2018-January 2019 at the Microbiology Laboratory of FMIPA, University of Lampung. This research using factorial completely randomized design with two factor treatment. The first factor is type of isolate (Mucor sp., Penicillium sp., IL3 (unidentified), Aspergillus sp.) and second faktor is dilution (control, 10 (without dilution), 10-1, 10-2, 10-3). Data were analyzed using ANOVA. If there are significant differences, then it is continued by the Duncan Test at the level of 5%. The results showed that the four types of fungi (Mucor sp., Penicillium sp., Aspergillus sp. and IL3) were able to cause mortality of Ae. aegypti mosquitoes with the highest killing power was  in Mucor sp. 10 (without dilution) is 43.33%. But the most effective is Mucor sp. 10-3 because the highest dilution has been able to cause mosquito mortality by 30%.  Key words: Ae. aegypti, DHF, entomopathogenic fungi, isolate, dilution. 

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