scholarly journals Antiproliferatif Ekstrak Metanol Daun Dianella nemorosa Lam. (Liliaceae) terhadap Sel Kanker MKN45 dengan Menggunakan Metode WST-1

2018 ◽  
Vol 3 (2) ◽  
pp. 47-52
Author(s):  
Aditya K. Karim ◽  
Sismindari Sismindari
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Inhibitory Effect ◽  
Antiproliferative Effect ◽  
Monosodium Salt ◽  
Mkn45 Cell

Dianella nemorosa Lam. was known containing alkaloids, terpenoid, phenolic compouds and tanin. Antiproliferative effect of D. nemorosa leaves methanol exctract, which demonstrated to have an in vitro cytotoxic effect on cancer cell line. The aim of research was examined the effect antiproliferative methanol extract of D. nemorosa leaves against MKN45 (gastric cancer) cell line. Leaves powder extracted using methanol. Antiproliferative effect was determined by Cell Proliferation Reagents WST-1 ((2-(4-iodophenyl)-3-(4-nitro-phenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt). Test for 1h, 2h, and 4h after incubated for 72h was done. The result of this research showed that methanol exctract from D. nemorosa possessed remarkable no had antiproliferative activity against MKN45 cell line. The result indicated methanol extract of D. nemorosa leaves selective inhibitory effect or antiproliferative against cell line. Key words: Dianella nemorosa, Antiproliferative, MKN 45, and WST-1

scholarly journals microRNA-32 inhibits the proliferation and invasion of the SGC-7901 gastric cancer cell line in vitro

2013 ◽  
Vol 7 (1) ◽  
pp. 270-274 ◽  
Author(s):  
JIANFENG ZHANG ◽  
XIAOLING KUAI ◽  
MENGJIAO SONG ◽  
XIAOQI CHEN ◽  
ZHIHUA YU ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Proliferation And Invasion

scholarly journals Cytosolic Cl- Affects the Anticancer Activity of Paclitaxel in the Gastric Cancer Cell Line, MKN28 Cell

2017 ◽  
Vol 42 (1) ◽  
pp. 68-80 ◽  
Author(s):  
Sachie Tanaka ◽  
Hiroaki Miyazaki ◽  
Atsushi Shiozaki ◽  
Daisuke Ichikawa ◽  
Eigo Otsuji ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Cell Line ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Tubulin Polymerization ◽  
Pass Through

Background/Aims: Our previous study revealed that cytosolic Cl- affected neurite elongation promoted via assembly of microtubule in rat pheochromocytoma PC12D cells and Cl-–induced blockade of intrinsic GTPase enhanced tubulin polymerization in vitro. Paclitaxel (PTX) is a microtubule-targeted chemotherapeutic drug and stabilizes microtubules resulting in mainly blockade of mitosis at the metaphase-anaphase transition and induction of apoptosis. In the present study, we tried to clarify whether the cytosolic Cl- affected PTX ability to inhibit cell growth in the gastric cancer cell line, MKN28. Methods: To clarify the cytosolic Cl- action on PTX-induced cell death and metaphase-anaphase transition in the gastric cancer cell line, MKN28 cell, and PTX-induced tubulin polymerization, we performed cell proliferation assay, cytosolic Cl- concentration measurement, immunofluorescence microscopy, and in vitro tubulin polymerization assay. Results: The decline of cytosolic Cl- weakened the cytotoxic effect of PTX on cell proliferation of MKN28 cells, which could pass through the metaphase-anaphase transition. Moreover, in vitro PTX-induced tubulin polymerization was diminished under the low Cl- condition. Conclusions: Our results strongly suggest that the upregulation of cytosolic Cl- concentration would enhance the antitumor effect of PTX, and that the cytosolic Cl- would be one of the key targets for anti-cancer therapy.

Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohammad Mostakhdem Hashemi ◽  
Majid Marjani ◽  
Nahid Poursharifi ◽  
Abdoljalal Marjani
Keyword(s):  
Gastric Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Incubation Time ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Root Extract ◽  
Human Gastric Cancer

Abstract One of the cancer-related deaths is gastric cancer in this area. Onosma dichroanthum Boiss. roots have been used as an antiseptic and anti-inflammatory for wound healing treatment. The aim of this study was to assess the in vitro cytotoxic and anticancer effects of O. dichroanthum Boiss. roots from the Golestan province of Iran. After identification of the taxonomical effect of O. dichroanthum Boiss., different concentrations of the hydroalcoholic root extract were used. Three different time periods (24, 48, and 72 h) were used to treat AGS gastric cancer and L-929 normal fibroblasts cell lines. The evaluation of different concentrations of root extract was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The 48 h treatment affected cell survival, while the concentration of 64 μg/mL was determined as IC50 concentrations at 48 h incubation time. The 48 h incubation time with 64 μg/mL showed the best effectiveness on cancerous cell-line while being safe for normal cell-line. Our results show that O. dichroanthum Boiss. roots extract may have cytotoxic and safe effects on gastric cancer cell-line and normal cells in 48 h treatment periods, respectively. The results indicated the O. dichroanthum Boiss. may be as an effective anticancer agent (gastric cancer).

scholarly journals Investigation of the Apoptotic Effects of Pistacia vera Nut Skin Methanol Extract on Human Gastric Cancer Cell Line HGC-27

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1525
Author(s):  
Abdullah Tuncay Demiryürek ◽  
Ahmet Saracaloglu ◽  
Ebru Temiz ◽  
Seniz Demiryürek ◽  
Bilge Sener
Keyword(s):  
Gastric Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Methanol Extract ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Human Gastric Cancer ◽  
Pistacia Vera ◽  
Apoptotic Effects

Gastric cancer (GC) is one of the most prevalent malignant types in the world and an aggressive disease with a poor 5-year survival. GC is the third greatest global cause of cancer-related deaths. It is well recognized that tumor heterogeneity, a fundamental feature of malignancy, plays an essential role in the cancer development and chemoresistance. In this study, we investigated the apoptotic effects of Pistacia vera nut skin methanol extract on human gastric cancer cell line HGC-27. The results of this study demonstrate for the first time the protective effect of Pistacia vera nut skin methanol extract on gastric carcinoma.

Bombesin stimulates the in vitro growth of a human gastric cancer cell line

1994 ◽  
Vol 161 (3) ◽  
pp. 519-525 ◽  
Author(s):  
Richard J. Bold ◽  
Patrick S. Lowry ◽  
Jin Ishizuka ◽  
James F. Battey ◽  
Courtney M. Townsend ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Human Gastric Cancer ◽  
In Vitro Growth ◽  
Human Gastric Cancer Cell
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