scholarly journals Granul Ekstrak Kayu Secang (Caesalpinia sappan L.) dan Rimpang Temu Mangga (Curcuma mangga Val & Zijp.) sebagai Antibakteri

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Nelly Uyo ◽  
Swasono R. Tamat ◽  
Kosasih Kosasih
Keyword(s):  
Antibacterial Activity ◽  
Quality Standard ◽  
Raw Material ◽  
Activity Data ◽  
Caesalpinia Sappan ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Powder Extract ◽  
One Way Anova

Kayu secang (Caesalpinia sappan) and rimpang temu mangga (Curcuma mangga) are medicinal plants that are efficacious for diarrhea due to bacterial infection. Kayu secang and Rimpang temu mangga contain secondary metabolite compounds capable of providing antibacterial activity, ie flavonoids. This study aimed to obtain the granule as supplies, which contain a combination of kayu secang and rimpang temu mangga with good quality, it is favored by consumers and has high antibacterial activity. Both powdered simplisia powder was kinetic with ethanol 70 % and dried by freeze drying method. Both powder extract tested the quality of raw material and test of antibacterial activity against bacteria Escherichia coli, Salmonella sp and Shigella sp. Both powder extracts were made granular preparations with the addition of excipients (Handbook of Pharmaceutical Excipients). The granule preparations were tested for granule quality standards and preferred levels and antibacterial activity. Data were analyzed statistically with one way ANOVA method (p= 0.05). Rimpang temu mangga extract powder added maltodextrin did not provide antibacterial activity. The granule extract of Kayu secang extract of formula 1 (F1) meets the requirements of granule quality standard, preferably consumer and has high antibacterial activity, i.e 18.7 mm against E. coli bacteria, 28.3 against Salmonella sp bacteria and 30.5 against Shigella sp bacteria.  Key words: Secang, C. sappan, temu mangga, extract, granule, antibacterial

STUDI PERBANDINGAN KUALITAS AIR BERSIH DALAM KAITANYA DENGAN AKTIVITAS MASYARAKAT DI SEKITAR SUMBER AIR PADA DESA WAINITU, BATUMERAH, AMAHUSU, DAN HALONG

2017 ◽  
Vol 13 (2) ◽  
pp. 111-119
Author(s):  
Lela Uyara ◽  
Pieter Kunu ◽  
Silwanus M Talakua
Keyword(s):  
Water Quality ◽  
Quality Standard ◽  
Total Coliform ◽  
Quality Analysis ◽  
Clean Water ◽  
Water Quality Analysis ◽  
E Coli ◽  
Microbiological Parameters ◽  
Standard Quality

The study aims to determine the quality of clean water in the villages of Wainitu, Batumerah, Amahusu and Halong by comparing the result of water quality analysis with water quality standard. Water quality analysis includes Physiscal, Chemical, and Microbiological parameters. This research uses descriptive method, this method describes systematics, accurate about facts and characteristic of the quality of clean water of each research location. The results showed that the source of clean water in the village of Batumerah did not meet the standard of clean water quality standards indicated by the number of E. coli and the high total coliform.  Keywords: standard quality of clean water, water quality, Wainitu, Batumerah, Amahusu and Halong villages   ABSTRAK Penelitian yang bertujuan untuk menetapkan kualitas air bersih di Desa Wainitu, Batumerah, Amahusu dan Halong, dengan membandingkan hasil analisis kualitas air dengan standar baku mutu air bersih. Analisis kualitas air meliputi parameter fisika, kimia dan mikrobiologi. Penelitian ini menggunakan metode deskriptif; metode ini menggambarkan sicara sistematis, akurat, fakta dan karakteristik mengenai kualitas air bersih di masing-masing lokasi penelitian. Hasil penelitian menunjukkan bahwa sumber air bersih di Desa Batumerah tidak memenuhi standar baku mutu air bersih yang ditunjukkan oleh jumlah E. coli dan total Koliform yang tinggi. Kata Kunci: baku mutu air bersih, Desa Wainitu, Batumerah, Amahusu dan Halong, kualitas air

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 90-97
Author(s):  
Gaby Maulida Nurdin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Lantana Camara ◽  
Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Growth Inhibitory ◽  
One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn)  on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

scholarly journals Evaluation Microbiologique Et Origines De La Contamination Des Produits De 4ème Gamme Vendus Sur Les Marchés D’abidjan, Cote D'ivoire

2016 ◽  
Vol 12 (36) ◽  
pp. 273
Author(s):  
Louise Atchibri Anin ◽  
Patrice Désiré Yapi Assoi Yapi ◽  
Yapo Thierry Monnet ◽  
Marie-Ange Yiwo Yapi ◽  
Chantal Lêniféré Soro ◽  
...  
Keyword(s):  
Food Contamination ◽  
Poor Quality ◽  
Raw Material ◽  
Ananas Comosus ◽  
Salmonella Spp ◽  
E Coli ◽  
Quality Of Water ◽  
Tomato Puree ◽  
The City

The sources and degree of microbial contamination of 4 th range products or ready-to-eat products made from traditional processes were evaluated in three markets in the city of Abidjan. This study initially consisted of searching for microorganisms such as Staphylococcus aureus, faecal coliforms, E. coli and Salmonella spp. Then, Ishikawa's method or diagram was used to determine potential sources of food contamination consisting of three (3) fruit and two (2) fruit vegetables and one (1) leafy vegetable, respectively, pineapple (Ananas comosus) papaya (Carica papaya), watermelon (Citrulus lanatus), onion (Allium cepa), tomato (Lycopersicum esculentum Mill.) and amaranthus (Amaranthus hybridus). Fecal coliform loads were very high at the fruit level (1.8 × 103 - 9.1 × 102 CFU / g), onion (1.3 × 104 CFU / g), tomato puree (1, 82 × 103 CFU / g) and an E. coli load at the tomato puree which is 9.1 × 102 CFU / g. The analysis using the Ishikawa diagram shows that contamination sources were the poor quality of water, raw material, lack of sanitation, illiteracy, unsafe traditional practices and sellers hygiene.

High strength and antibacterial polyelectrolyte complex CS/HS hydrogel films for wound healing

Soft Matter ◽  
2019 ◽  
Vol 15 (38) ◽  
pp. 7686-7694 ◽  
Author(s):  
Mengmeng Shu ◽  
Shijun Long ◽  
Yiwan Huang ◽  
Dapeng Li ◽  
Haiyan Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Antibacterial Activity ◽  
Polyelectrolyte Complex ◽  
High Strength ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Non Covalent Interactions ◽  
Covalent Interactions ◽  
Hydrogel Films

Hydrogels with high strength were obtained based on non-covalent interactions. High antibacterial activity against E. coli was archived, and wound healing was remarkably facilitated through complexation of two functional polysaccharides.

KONDISI KUALITAS AIR (ASPEK MIKROORGANISME) DI PERAIRAN SEKITAR PULAU BUNAKEN, SULAWESI UTARA

2017 ◽  
Vol 5 (3) ◽  
pp. 94
Author(s):  
Roberto V Larat ◽  
Markus T Lasut ◽  
Robert A Bara
Keyword(s):  
Escherichia Coli ◽  
Sea Water ◽  
Liquid Waste ◽  
Quality Standard ◽  
Marine Biota ◽  
Sampling Point ◽  
E Coli ◽  
The Status ◽  
Comparison Table

In general, water conditions surrounding Bunaken Island are not separated from the liquid waste disposal, for example: bathing, washing, latrines, and landfills. Based on this, the research was conducted to determine the content of microorganisms Escherichia coli and coliform derived from liquid waste around Bunaken Island. The result showed the concentrations of E. coli were point (TS) I (<1 MPN / 100 ml), II (<1 MPN / 100 ml), and III (<1 MPN / 100 ml), and at the sampling point (TS) IV (1.0 MPN / 100 ml), V (1.0 MPN / 100 ml), and VI (1.0 MPN / 100 ml). Furthermore, coliform concentration has the similar valueat each sampling points (TS) I, II, III, IV, V, and VI with the amount of 2.0 MPN / 100 ml. The status around the Bunaken island on the comparison table between the this results and the standards of the Sea Quality Standard for Marine Tourism still qualifies or does not exceed the limits which stated in the creed of Minister ofEnviromental Affair Republic of Indonesia number 179 of 2004. Followed by Creed of Minister ofEnviromental Affair number 51 of 2004 on the Quality of Raw Water for Marine Biota, is categorized as the "proper" one of sea water quality for marine biota on the based ofEscherichia coli and coliform ratio.Keywords: Escherichia coli, coliform, air quality, Bunaken Island, North SulawesiPada umumnya, kondisi perairan yang berada di sekitar Pulau Bunaken tidak terlepas dari pembuangan limbah cair, misalnya : mandi, cuci dan kakus, serta tempat pembuangan sampah. Bertolak dari hal tersebut, penelitian ini dilakukan untuk mengkaji keberadaan kualitas limbah cair dalam aspek mikroorganisme di perairan sekitar pulau Bunaken dengan mengukur konsentrasi Escherichia coli dan coliform.Konsentrasi Escherichia coli berturut-turut pada titik sampling (TS) I (<1 MPN/100 ml), II (<1 MPN/100 ml) , dan III (<1 MPN/100 ml), dan pada titik sampling (TS) IV (1,0 MPN/100 ml), V (1,0 MPN/100 ml) , dan VI (1,0 MPN/100 ml). Coliformmemiliki jumlah konsentrasi yang sama, yaitu berturut-turut pada titik sampling (TS) I, II, III, IV, V, dan VI dengan jumlah 2.0 MPN/100 ml.Status perairan sekitar pulau Bunaken pada tabel perbandingan antara hasil pengukuran dan standart Baku Mutu Air Laut Untuk Wisata Bahari, masih memenuhi syarat atau tidak melewati batas yang ditetapkan dalam KEPMEN L.H. No.179 tahun 2004.Sedangkan berdasarkan KEPMEN L.H. No.51 Tahun 2004 tentang Baku Mutu Air Laut Untuk Biota Laut, berada dalam kategori “layak” sebagai peruntukan air laut untuk biota laut, berdasarkan parameter Escherichia coli dan coliform.Kata Kunci :Escherichia coli, coliform, kualitas air, Pulau Bunaken, Sulawesi Utara

scholarly journals 5-Bromo-1-(4-chlorobenzyl)-1H-indole-2-carboxamides as new potent antibacterial agents

2018 ◽  
Vol 24 (6) ◽  
pp. 327-332 ◽  
Author(s):  
Yogesh D. Mane ◽  
Smita S. Patil ◽  
Dhanraj O. Biradar ◽  
Bhimrao C. Khade
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antibacterial Agents ◽  
Inhibitory Concentration ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Internal Standards ◽  
Gram Negative ◽  
E Coli ◽  
High Antibacterial Activity

Abstract Ten 5-bromoindole-2-carboxamides were synthesized, characterized and evaluated for antibacterial activity against pathogenic Gram-negative bacteria Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Salmonella Typhi using gentamicin and ciprofloxacin as internal standards. Compounds 7a–c, 7g and 7h exhibit high antibacterial activity with a minimum inhibitory concentration (MIC) of 0.35–1.25 μg/mL. Compounds 7a–c exhibit antibacterial activities that are higher than those of the standards against E. coli and P. aeruginosa.

Antibacterial properties of Ag and Ag/AgCl nanoparticles from radish and tea extracts for water treatment applications

2015 ◽  
Vol 16 (1) ◽  
pp. 171-179
Author(s):  
Yuphada Boonto ◽  
Jirapat Ananpattarachai ◽  
Puangrat Kajitvichyanukul
Keyword(s):  
Antibacterial Activity ◽  
Water Treatment ◽  
Microwave Irradiation ◽  
Green Synthesis ◽  
Plant Extracts ◽  
Antibacterial Properties ◽  
Water Disinfection ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Complete Inactivation

Silver nanoparticles (AgNPs) have antibacterial properties and are widely used for water disinfection. This technology is commercially applied in point-of-use water treatment as a post-treatment for filtrate water. However, the current process of synthesizing AgNPs has several disadvantages including the use of hazardous chemicals, consumption of a large amount of energy and the formation of hazardous byproducts. Here, we report an alternative and green synthesis using plant extracts. In this work, the plant extracts came from radish (R) and tea (T), and the AgNPs were derived from a microwave irradiation method. The AgNPs synthesized by chemical-based microwave irradiation (Ag-C) were also used as a control material. The novel method produced a smaller size of nanostructures with good dispersion ability and less agglomeration than those from chemical synthesis. The antibacterial properties of AgNPs on Gram-negative bacteria Escherichia coli (E. coli) and Gram-positive bacteria Staphylococcus aureus (S. aureus) were investigated. The results revealed that AgNPs from both green synthesis and chemical-based methods inactivated both types of bacteria. The green-synthesized AgNPs from radish juice provided a higher percentage of inhibition of E. coli than that of S. aureus. The inactivation rates of the AgNPs increased with increasing concentration of AgNPs. As the concentration of the Ag/AgCl-R and Ag-R increased from 150 μg/mL to 300 μg/mL, complete inactivation required a reduced time for the reaction from 300 minutes to only 30 minutes. Finally, the Ag/AgCl-R and Ag-R offered high antibacterial activity while the Ag-T provided the lowest antibacterial activity. This work provides an alternative method for the eco-synthesis of antibacterial nanomaterials for water treatment.

scholarly journals Structures and Antibacterial Properties of PLA-based Ciprofloxacin Composite Films Deposited by Low-Electron Beam Dispersion

2020 ◽  
Vol 22 (1) ◽  
pp. 35 ◽  
Author(s):  
Beibei Li ◽  
Chun He ◽  
Xiaohong Jiang ◽  
M.A. Yarmolenko ◽  
D.G. Piliptsou ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Antibacterial Activity ◽  
Electron Beam ◽  
Agar Medium ◽  
Composite Films ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Mass Ratio ◽  
E Coli ◽  
High Antibacterial Activity

Polylactic acid (PLA)-based ciprofloxacin (Cip) antibacterial films with mass ratio PLA:Cip=1:1 were prepared by low-electron beam dispersion (EBD). The molecular structure, chemical composition and morphology of PLA-based ciprofloxacin antibacterial films were investigated by XPS, FTIR, liquid NMR and SEM. The antibacterial activity of composite films was tested against E. coli ATCC 25922 and S. aureus ATCC 12600 using the agar diffusion method on the solid LB agar medium. XPS and FTIR analysis showed the presence of an antibacterial ingredient in the composite films. Using NMR, it has been shown that the molecular structure of a monolayer of ciprofloxacin is fully consistent with the molecular structure of the initial ciprofloxacin powders. High antibacterial activity of the composite films has been also established and the layers still possess antibacterial activity with regard to S. aureus even after 7 days of leaching in an isotonic solution. The thermal treatment indicates that the composite films can withstand temperatures of 180 °C and keep its structure unchanged.

scholarly journals Schiff Base of Metal Complex Derived from Glycine and Benzaldehyde

2020 ◽  
pp. 105-114
Author(s):  
Ishaq Yahaya Lawan ◽  
Mohammed Muftahu Beli ◽  
Mohammed Adamu ◽  
Fatima Baba Isah ◽  
Maryam Abubakar
Keyword(s):  
Antibacterial Activity ◽  
Schiff Base ◽  
Metal Complex ◽  
Schiff Base Ligand ◽  
Conductivity Measurement ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Ir Analysis

Schiff base ligand derived from glycine and benzaldehyde was synthesized together with its metal complexes (zinc and cobalt). The solubility, IR analysis and conductivity measurement were carried out. Antibacterial activities were evaluated using well-diffusion method. The bacterial assay was carried out on two pyogenic bacteria E. coli and Staphylococci and the results showed that the complexes have high antibacterial activity.

scholarly journals Antibacterial activity of Acanthus sennii extracts against Staphylococcus aureus and Escherichia coli pathogens

2020 ◽  
Vol 13 (2) ◽  
pp. 99-113
Author(s):  
Kindu Geta ◽  
Mulugeta Kibret
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Microbial Infections ◽  
The Mean ◽  
Ethanol Extracts

Medicinal plants offer a major and accessible source of health care to people living in developing countries. Increasing drug resistant microbial infections intensified the search for new, safer, and more efficacious agents against microbial infections. Acanthus sennii is one of the medicinal plants used traditionally for the treatment of different infectious diseases in Ethiopia. Therefore, this study was carried out to evaluate antibacterial activity of A. sennii against pathogenic bacteria. Plant materials were extracted by maceration technique with chloroform, ethanol and water solvents. The antibacterial activities of the crude extracts of the plant were carried out by the agar well diffusion method. Broth dilution method was used to determine minimum inhibitory and streak plate bactericidal concentration of extracts. The results revealed that ethanol extracts of leaves revealed high antibacterial activity against standard strains of Staphylococcus aureus with inhibition zone of 14±0.6 mm at 25 mg/ml and 17±0.7 mm at 50 mg/ml. Ethanol extracts of buds showed high antibacterial activity against standard strains of S. aureus with inhibition zone of 25.7±0.7 mm at 100 mg/ml, also against standard strains of E. coli with inhibition zone of 16 mm at 50 mg/ml and 23.7 mm at 100 mg/ml. The mean minimum inhibitory concentration of 5.2±1.8 and 2.6±0.5 mg/ml was recorded for ethanol extract of leaves against standard strains of E. coli and clinical isolates of S. aureus; the mean minimum bactericidal concentration of 4.2±1.0 mg/ml with ethanol extract of leaves against standard strains of S. aureus; and the mean minimum bactericidal concentration of 12.5 mg/ml against standard strains and clinical isolates of E. coli. The result showed that A. sennii could be a candidate in the search for new antibacterial agents against these bacteria and its use in ethnomedicinal treatment of infectious diseases used by local communities may be validated. Isolating bio-active components and determining toxicity are future agenda. Keywords: Acanthus sennii, Antibacterial activity, E. coli, Minimum inhibition concentration, Minimum bactericidal concentration, S. aureus  

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