High strength and antibacterial polyelectrolyte complex CS/HS hydrogel films for wound healing

Soft Matter ◽  
2019 ◽  
Vol 15 (38) ◽  
pp. 7686-7694 ◽  
Author(s):  
Mengmeng Shu ◽  
Shijun Long ◽  
Yiwan Huang ◽  
Dapeng Li ◽  
Haiyan Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Antibacterial Activity ◽  
Polyelectrolyte Complex ◽  
High Strength ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Non Covalent Interactions ◽  
Covalent Interactions ◽  
Hydrogel Films

Hydrogels with high strength were obtained based on non-covalent interactions. High antibacterial activity against E. coli was archived, and wound healing was remarkably facilitated through complexation of two functional polysaccharides.

scholarly journals Binding From both sides: TolR and full-length OmpA bind and maintain the local structure of the E. coli cell wall

2018 ◽  
Author(s):  
Alister T. Boags ◽  
Firdaus Samsudin ◽  
Syma Khalid
Keyword(s):  
Cell Wall ◽  
Electrostatic Interactions ◽  
Cell Envelope ◽  
Binding Domain ◽  
Gram Negative Bacteria ◽  
Inner Membrane ◽  
E Coli ◽  
Non Covalent Interactions ◽  
Covalent Interactions

SUMMARYWe present a molecular modeling and simulation study of the of the E. coli cell envelope, with a particular focus on the role of TolR, a native protein of the E. coli inner membrane in interactions with the cell wall. TolR has been proposed to bind to peptidoglycan, but the only structure of this protein thus far is in a conformation in which the putative peptidoglycan binding domain is not accessible. We show that a model of the extended conformation of the protein in which this domain is exposed, binds peptidoglycan largely through electrostatic interactions. We show that non-covalent interactions of TolR and OmpA with the cell wall, from the inner membrane and outer membrane sides respectively, maintain the position of the cell wall even in the absence of Braun’s lipoprotein. When OmpA is truncated to remove the peptidoglycan binding domain, TolR is able to pull the cell wall down towards the inner membrane. The charged residues that mediate the cell-wall interactions of TolR in our simulations, are conserved across a number of species of Gram-negative bacteria.

scholarly journals 5-Bromo-1-(4-chlorobenzyl)-1H-indole-2-carboxamides as new potent antibacterial agents

2018 ◽  
Vol 24 (6) ◽  
pp. 327-332 ◽  
Author(s):  
Yogesh D. Mane ◽  
Smita S. Patil ◽  
Dhanraj O. Biradar ◽  
Bhimrao C. Khade
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antibacterial Agents ◽  
Inhibitory Concentration ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Internal Standards ◽  
Gram Negative ◽  
E Coli ◽  
High Antibacterial Activity

Abstract Ten 5-bromoindole-2-carboxamides were synthesized, characterized and evaluated for antibacterial activity against pathogenic Gram-negative bacteria Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Salmonella Typhi using gentamicin and ciprofloxacin as internal standards. Compounds 7a–c, 7g and 7h exhibit high antibacterial activity with a minimum inhibitory concentration (MIC) of 0.35–1.25 μg/mL. Compounds 7a–c exhibit antibacterial activities that are higher than those of the standards against E. coli and P. aeruginosa.

Antibacterial properties of Ag and Ag/AgCl nanoparticles from radish and tea extracts for water treatment applications

2015 ◽  
Vol 16 (1) ◽  
pp. 171-179
Author(s):  
Yuphada Boonto ◽  
Jirapat Ananpattarachai ◽  
Puangrat Kajitvichyanukul
Keyword(s):  
Antibacterial Activity ◽  
Water Treatment ◽  
Microwave Irradiation ◽  
Green Synthesis ◽  
Plant Extracts ◽  
Antibacterial Properties ◽  
Water Disinfection ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Complete Inactivation

Silver nanoparticles (AgNPs) have antibacterial properties and are widely used for water disinfection. This technology is commercially applied in point-of-use water treatment as a post-treatment for filtrate water. However, the current process of synthesizing AgNPs has several disadvantages including the use of hazardous chemicals, consumption of a large amount of energy and the formation of hazardous byproducts. Here, we report an alternative and green synthesis using plant extracts. In this work, the plant extracts came from radish (R) and tea (T), and the AgNPs were derived from a microwave irradiation method. The AgNPs synthesized by chemical-based microwave irradiation (Ag-C) were also used as a control material. The novel method produced a smaller size of nanostructures with good dispersion ability and less agglomeration than those from chemical synthesis. The antibacterial properties of AgNPs on Gram-negative bacteria Escherichia coli (E. coli) and Gram-positive bacteria Staphylococcus aureus (S. aureus) were investigated. The results revealed that AgNPs from both green synthesis and chemical-based methods inactivated both types of bacteria. The green-synthesized AgNPs from radish juice provided a higher percentage of inhibition of E. coli than that of S. aureus. The inactivation rates of the AgNPs increased with increasing concentration of AgNPs. As the concentration of the Ag/AgCl-R and Ag-R increased from 150 μg/mL to 300 μg/mL, complete inactivation required a reduced time for the reaction from 300 minutes to only 30 minutes. Finally, the Ag/AgCl-R and Ag-R offered high antibacterial activity while the Ag-T provided the lowest antibacterial activity. This work provides an alternative method for the eco-synthesis of antibacterial nanomaterials for water treatment.

scholarly journals Structures and Antibacterial Properties of PLA-based Ciprofloxacin Composite Films Deposited by Low-Electron Beam Dispersion

2020 ◽  
Vol 22 (1) ◽  
pp. 35 ◽  
Author(s):  
Beibei Li ◽  
Chun He ◽  
Xiaohong Jiang ◽  
M.A. Yarmolenko ◽  
D.G. Piliptsou ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Antibacterial Activity ◽  
Electron Beam ◽  
Agar Medium ◽  
Composite Films ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Mass Ratio ◽  
E Coli ◽  
High Antibacterial Activity

Polylactic acid (PLA)-based ciprofloxacin (Cip) antibacterial films with mass ratio PLA:Cip=1:1 were prepared by low-electron beam dispersion (EBD). The molecular structure, chemical composition and morphology of PLA-based ciprofloxacin antibacterial films were investigated by XPS, FTIR, liquid NMR and SEM. The antibacterial activity of composite films was tested against E. coli ATCC 25922 and S. aureus ATCC 12600 using the agar diffusion method on the solid LB agar medium. XPS and FTIR analysis showed the presence of an antibacterial ingredient in the composite films. Using NMR, it has been shown that the molecular structure of a monolayer of ciprofloxacin is fully consistent with the molecular structure of the initial ciprofloxacin powders. High antibacterial activity of the composite films has been also established and the layers still possess antibacterial activity with regard to S. aureus even after 7 days of leaching in an isotonic solution. The thermal treatment indicates that the composite films can withstand temperatures of 180 °C and keep its structure unchanged.

scholarly journals Schiff Base of Metal Complex Derived from Glycine and Benzaldehyde

2020 ◽  
pp. 105-114
Author(s):  
Ishaq Yahaya Lawan ◽  
Mohammed Muftahu Beli ◽  
Mohammed Adamu ◽  
Fatima Baba Isah ◽  
Maryam Abubakar
Keyword(s):  
Antibacterial Activity ◽  
Schiff Base ◽  
Metal Complex ◽  
Schiff Base Ligand ◽  
Conductivity Measurement ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Ir Analysis

Schiff base ligand derived from glycine and benzaldehyde was synthesized together with its metal complexes (zinc and cobalt). The solubility, IR analysis and conductivity measurement were carried out. Antibacterial activities were evaluated using well-diffusion method. The bacterial assay was carried out on two pyogenic bacteria E. coli and Staphylococci and the results showed that the complexes have high antibacterial activity.

scholarly journals Antibacterial activity of Acanthus sennii extracts against Staphylococcus aureus and Escherichia coli pathogens

2020 ◽  
Vol 13 (2) ◽  
pp. 99-113
Author(s):  
Kindu Geta ◽  
Mulugeta Kibret
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Microbial Infections ◽  
The Mean ◽  
Ethanol Extracts

Medicinal plants offer a major and accessible source of health care to people living in developing countries. Increasing drug resistant microbial infections intensified the search for new, safer, and more efficacious agents against microbial infections. Acanthus sennii is one of the medicinal plants used traditionally for the treatment of different infectious diseases in Ethiopia. Therefore, this study was carried out to evaluate antibacterial activity of A. sennii against pathogenic bacteria. Plant materials were extracted by maceration technique with chloroform, ethanol and water solvents. The antibacterial activities of the crude extracts of the plant were carried out by the agar well diffusion method. Broth dilution method was used to determine minimum inhibitory and streak plate bactericidal concentration of extracts. The results revealed that ethanol extracts of leaves revealed high antibacterial activity against standard strains of Staphylococcus aureus with inhibition zone of 14±0.6 mm at 25 mg/ml and 17±0.7 mm at 50 mg/ml. Ethanol extracts of buds showed high antibacterial activity against standard strains of S. aureus with inhibition zone of 25.7±0.7 mm at 100 mg/ml, also against standard strains of E. coli with inhibition zone of 16 mm at 50 mg/ml and 23.7 mm at 100 mg/ml. The mean minimum inhibitory concentration of 5.2±1.8 and 2.6±0.5 mg/ml was recorded for ethanol extract of leaves against standard strains of E. coli and clinical isolates of S. aureus; the mean minimum bactericidal concentration of 4.2±1.0 mg/ml with ethanol extract of leaves against standard strains of S. aureus; and the mean minimum bactericidal concentration of 12.5 mg/ml against standard strains and clinical isolates of E. coli. The result showed that A. sennii could be a candidate in the search for new antibacterial agents against these bacteria and its use in ethnomedicinal treatment of infectious diseases used by local communities may be validated. Isolating bio-active components and determining toxicity are future agenda. Keywords: Acanthus sennii, Antibacterial activity, E. coli, Minimum inhibition concentration, Minimum bactericidal concentration, S. aureus  

scholarly journals Towards a new treatment against polymicrobial infections: high antibacterial activity of lemon IntegroPectin against Pseudomonas aeruginosa and Escherichia coli

2020 ◽  
Author(s):  
Alessandro Presentato ◽  
Antonino Scurria ◽  
Lorenzo Albanese ◽  
Pasquale Picone ◽  
Mario Pagliaro ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Industrial Development ◽  
Antimicrobial Treatment ◽  
Hydrodynamic Cavitation ◽  
E Coli ◽  
High Antibacterial Activity ◽  
New Treatment ◽  
Polymicrobial Infections

AbstractLemon IntegroPectin obtained via hydrodynamic cavitation of waste lemon peel in water only shows high antibacterial activity against two Gram-negative bacteria, Pseudomonas aeruginosa and Escherichia coli. The antibacterial effect against the ubiquitous pathogen P. aeruginosa was evaluated in terms of the minimal bactericidal (MBC) and minimal inhibitory concentration (MIC). Preliminary insight on the antibacterial mechanism of IntegroPectin originates from investigating its inhibitory activity against E. coli. Given the non-cytotoxic nature of citrus IntegroPectin and the ease of its reproducible production in large amounts, the route is open to the industrial development of a new antimicrobial treatment against polymicrobial infections unlikely to develop drug resistance.

scholarly journals Microwave-Assisted Rapid Synthesis of Eu(OH)3/RGO Nanocomposites and Enhancement of Their Antibacterial Activity against Escherichia coli

Materials ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 43
Author(s):  
Kun-Yauh Shih ◽  
Shiou-Ching Yu
Keyword(s):  
Antibacterial Activity ◽  
Inhibition Zone ◽  
X Ray Diffraction ◽  
Inhibition Zone Diameter ◽  
X Ray ◽  
E Coli ◽  
Inhibition Effect ◽  
Microwave Assisted ◽  
High Antibacterial Activity ◽  
The Eu

Nanomaterials with high antibacterial activity and low cytotoxicity have attracted extensive attention from scientists. In this study, europium (III) hydroxide (Eu(OH)3)/reduced graphene oxide (RGO) nanocomposites were synthesized using a rapid, one-step method, and their antibacterial activity against Escherichia coli (E. coli) was investigated using the synergistic effect of the antibacterial activity between Eu and graphene oxide (GO). The Eu(OH)3/RGO nanocomposites were prepared using a microwave-assisted synthesis method and characterized using X-ray diffraction, scanning electron microscopy, photoluminescence spectroscopy, Raman spectroscopy, and Fourier-transform infrared spectroscopy. Raman sprectroscopy and X-ray diffraction confirmed the pure hexagonal phase structure of the nanocomposites. Further, the antibacterial properties of Eu(OH)3/RGO were investigated using the minimum inhibitory concentration assay, colony counting method, inhibition zone diameter, and optical density measurements. The results revealed that the Eu(OH)3/RGO exhibited a superior inhibition effect against E. coli and a larger inhibition zone diameter compared to RGO and Eu(OH)3. Further, the reusability test revealed that Eu(OH)3/RGO nanocomposite retained above 98% of its bacterial inhibition effect after seven consecutive applications. The high antibacterial activity of the Eu(OH)3/RGO nanocomposite could be attributed to the release of Eu3+ ions from the nanocomposite and the sharp edge of RGO. These results indicated the potential bactericidal applications of the Eu(OH)3/RGO nanocomposite.

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