Pengendalian Infeksi Bakteri Aeromonas hydrophila pada Ikan Nila (Oreochromis niloticus) dengan Ekstrak Rimpang Lengkuas Merah (Alpinia purpurata)

Endang T.P. Sari; Tri Gunaedi; Ervina Indrayani

doi:10.31957/jbp.110

Pengendalian Infeksi Bakteri Aeromonas hydrophila pada Ikan Nila (Oreochromis niloticus) dengan Ekstrak Rimpang Lengkuas Merah (Alpinia purpurata)

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.110 ◽

2018 ◽

Vol 9 (2) ◽

pp. 37-42

Author(s):

Endang T.P. Sari ◽

Tri Gunaedi ◽

Ervina Indrayani

Keyword(s):

Experimental Research ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Positive Control ◽

Negative Control ◽

Bacterial Activity ◽

Clear Zone ◽

Motile Aeromonas Septicaemia ◽

Alpinia Purpurata ◽

Active Substances

MAS diseases (Motile Aeromonas Septicaemia) is caused by the bacterium Aeromonas hydrophila. One of the natural ingredients that can be used as an alternative to inhibit bacterial activity is the extract of red galangal rhizome (Alpinia purpurata). Active substances possessed of this material have potential as antibacterial. The purpose of this study is to determine the effectiveness of red galangal rhizome extract in the control of MAS disease in tilapia. The type of this research is experimental research in Completely Random Design (RAL) with 6 treatments, which are positive control, negative control, 250, 500, 750, and 1000 ppm. The result of the research on the activity of the galangal extract bacteria on A. hydrophila obtained the widest clear zone at 1000 ppm constipation, which is 9.7625 mm. The treatment has been conducted by immersion of Tilapia fish into the extracted red galangan rgizomes for 5 minutes, then it was put into aquarium for maintenance for 7 days. 0.1 ml A. hydrophila (108 cfu/ml) was injected to Tilapia. The result of the research on antibacterial shoes the wides clear zone at 1000 ppm with constipation, which is 9.7625 mm and the effectiveness test of red galangal extract on tilapia infected by A. hydrophila bacteria experience (ulcer) healing after 7 days with the concentration of rhizome extract of galangal 1000 ppm. Key words: red galangal rhizome extract, A. hydrophila, A. purpurata.

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Efikasi daun sembukan Paederia foetida untuk pencegahan infeksi bakteri Aeromonas hydrophila pada ikan nila

Jurnal Akuakultur Indonesia ◽

10.19027/jai.15.108-119 ◽

2016 ◽

Vol 15 (2) ◽

pp. 108 ◽

Cited By ~ 1

Author(s):

Dinamella Wahjuningrum ◽

Mulyati Hasanah ◽

, Rahman

Keyword(s):

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Preventive Treatment ◽

Positive Control ◽

Negative Control ◽

Optimum Dosage ◽

Coating Method ◽

Control And Prevention ◽

Paederia Foetida

ABSTRACT The purpose of this study was to determine the optimum dosage of skunkvine leaves Paederia foetida to prevent infection caused by bacteria Aeromonas hydrophila in tilapia Oreochromis niloticus. This study consisted of five treatments. They were negative control, positive control, and prevention treatment with the dosages of 0.8%, 1% and that 1.2% that consisted that of three replications in each treatment. Addition of skunkvine leaves on feed performed by coating method. Feed was given at satiation with a frequency of three times a day. The results of this study showed that there were significant effect (P<0.05) between the positive control (37.03%) and preventive treatment dosages of 0.8% (88.89%), 1% (74.08%), and 1.2% (74.08%). The optimum dosage for prevention of A. hydrophila infection in tilapia was 0.8%. Keywords: Aeromonas hydrophila, Paederia foetida, tilapia ABSTRAK Tujuan penelitian ini adalah untuk menentukan dosis daun sembukan Paederia foetida yang tepat dalam mencegah infeksi akibat Aeromonas hydrophila pada ikan nila Oreochromis niloticus. Penelitian ini terdiri dari lima perlakuan yaitu kontrol negatif, kontrol positif, perlakuan 0,8%, perlakuan 1%, dan perlakuan 1,2% dengan masing-masing perlakuan terdiri dari tiga ulangan. Metode penambahan sembukan pada pakan dilakukan dengan metode coating. Pakan diberikan secara at satiation dengan frekuensi pemberian sebanyak tiga kali sehari. Pemeliharaan ikan dilakukan selama 20 hari dan pada hari ke-11 dilakukan uji tantang dengan menggunakan bakteri A. hydrophila. Hasil penelitian ini menunjukan bahwa tingkat kelangsungan hidup sebelum uji tantang tidak berbeda nyata (P>0,05), sedangkan setelah uji tantang diperoleh hasil yang berbeda nyata (P<0,05) antara kontrol positif (37,03%) dengan perlakuan 0,8% (88,89%), perlakuan 1% (74,08%), dan perlakuan 1,2% (74,08%). Berdasarkan hasil penelitian disimpulkan bahwa pemberian daun sembukan melalui pakan efektif untuk pencegahan infeksi A. hydrophila pada ikan nila dengan dosis terbaik yaitu 0,8%. Kata kunci: Aeromonas hydrophila, Paederia foetida, ikan nila

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The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.0.0.2014.7302 ◽

2014 ◽

pp. 27

Author(s):

Arne A Ratulangi ◽

Reiny Tumbol ◽

Hengky Manoppo ◽

Henneke Pangkey

Keyword(s):

Survival Rate ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Bacterial Disease ◽

Immersion Method ◽

Nile Tilapia Oreochromis Niloticus ◽

Different Ages ◽

Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

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AKTIVITAS HEPATOPROTEKTOR SARI BUAH LABU KUNING (Cucurbita moschata Duchesne.) PADA TIKUS (Rattus norvergicus) YANG DIINDUKSIKAN KARBON TETRAKLORIDA

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2016.v2.i1.5226 ◽

2016 ◽

Vol 2 (1) ◽

pp. 18-23

Author(s):

Indrawan Aditya ◽

Ihwan Ihwan ◽

Jamaluddin Jamaluddin

Keyword(s):

Experimental Research ◽

Beta Carotene ◽

Positive Control ◽

Negative Control ◽

The Body ◽

Cucurbita Moschata ◽

The Third ◽

Body Tissues ◽

Risk Of Cancer ◽

Carotene Beta

One of the plants that are often used in traditional medicine is the fruit of pumpkin used to treat hepatitis manner pumpkin fruit cut into small pieces and then steamed, then eaten .Pumpkin fruits contain active compounds such as saponins, tannins, flavonoids and beta-carotene. Beta-carotene is converted in the body into vitamin A is beneficial for the growth, maintenance of body tissues and to reduce the risk of cancer and heart disease. This study aims to determine pumpkin juice has a hepatoprotective activities on liver function by looking at parameters SGPT and SGOT in rats after induced CCl4. Type of experimental research using True Experimental research design design. Test animals were grouped into 5 groups, each group consisting of 3 rats. The first group of positive control (Methicol®), the second group negative control (CCl4), the third group of pumpkin juice 25%, the fourth group of pumpkin juice 50%, the fifth group of pumpkin juice 75%. From the results of this study concluded that the pumpkin juice 75% effective as a hepatoprotective by looking at parameters SGPT is 48 U/liter and SGOT is 66 U/liter in rats after induced carbon tetrachloride

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PENGGUNAAN EKSTRAK DAUN SIRIH (Piper betle Linn) UNTUK MENGHAMBAT BAKTERI Aeromonas hydrophila DAN TOKSISITASNYA PADA IKAN PATIN (Pangasius hypophthalmus) (THE USE OF PIPER BETLE LINN EXTRACT ON AEROMONAS HYDROPHILA TO OBSTRUCT AND THE TOKSISITY TO PANGASIUS HYPOPHTHALMUS)

Fish Scientiae ◽

10.20527/fs.v1i2.1188 ◽

2016 ◽

Vol 1 (2) ◽

pp. 190 ◽

Cited By ~ 1

Author(s):

Siti Aisiah ◽

Muhammad Muhammad ◽

Anita Anita

Keyword(s):

Aeromonas Hydrophila ◽

Random Sampling ◽

Toxicity Test ◽

Methanol Extract ◽

Positive Control ◽

Negative Control ◽

Quality Parameter ◽

Control Fish ◽

Water Quality Parameter ◽

Piper Betle

Tujuan penelitian ini untuk mengetahui daya hambat daun sirih yang paling besar terhadap bakteri Aeromonas Hydrophila, mengetahui konsentrasi minimal ekstrak daun sirih yang mampu menghambat pertumbuhan bakteri A. hydrophila dan mengetahui toksisitas konsentrasi efektif dari ekstrak daun sirih terhadap ikan patin. Rancangan percobaan yang digunakan untuk uji toksisitas adalah rancangan acak lengkap, terdiri dari 4 perlakuan yaitu A = Ikan disuntik dengan ekstrak daun sirih konsentrasi 75%, B = Ikan disuntik dengan ekstrak daun sirih konsentrasi 25%, C = Kontrol positif (ikan disuntik dengan akuades steril) dan D = Kontrol negatif (ikan tidak disuntik), diulang 3 kali. Hasil uji sensitivitas antibakteri daun sirih yang mempunyai daya hambat dan daya bunuh paling besar terhadap bakteri A. hydrophila adalah ekstrak daun sirih-metanol. Pengujian MIC menunjukkan bahwa ekstrak daun sirih-metanol memiliki daya hambat minimal 25 % terhadap aktivitas bakteri A. hydrophila. Hasil uji toksisitas yang dilakukan terhadap ikan patin dengan konsentrasi 75% dan 25% menunjukkan bahwa mortalitas yang terjadi tidak mencapai 50%. Pengamatan hematologis pada masing-masing perlakuan menunjukkan hasil yang berpengaruh tidak nyata terhadap kesehatan ikan patin. Parameter kualitas air pada penelitian ini yaitu, , kadar oksigen terlarut, pH, amoniak, CO2 dan suhu masih dapat mendukung kehidupan ikan patin.This research was aimed a finding the part of Piper betle Linn which had the biggest resistance to Aeromonas hydrophila bacteria and to know the minimal concentrate which could obstruct the growth of A. hydrophila bacteria and to know effective concentrate toxicity of P. betle Linn to Pangasius hypophthalmus. The random sampling used proportionate stratified random sampling. In toxicity test, it had be done 4 treatment, which was given to fish, those were : A = fish was injected with 25 % concentrate of extract P. betle Linn leaves, B = fish was injected with 75 % concentrate of extract P. betle Linn leaves, C = positive control (fish was injected with sterile aquadest), and D = negative control (fish wasn’t injected) and 3 trial. Sensivity result of A. hydrophila bacteria to P. betle Linn showed that the axtract of Piper betle Linn leaves-methanol had bigger activity than others. Depended on MIC test of the leaves P. betle Linn-methanol extract showed result that the extract had 25% minimal bloked concentrate to A. hydrophila bacteria. The result of toxicity test of P. betle Linn 75% and 25% leaves was no mortality 50% of P. hypophthalmus. Water quality parameter during experiment like dissolving oxygen, pH, ammonia, CO2, and temperature were still in reasonable range for Pangasius hypophthalmus.

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The Effectiveness Of Anchovy Concentration (Stolephorus insularis) as Antimicrobial to Streptococcus mutans (In Vitro)

DENTA ◽

10.30649/denta.v10i1.37 ◽

2016 ◽

Vol 10 (1) ◽

pp. 71

Author(s):

Almira Fa’Izah ◽

Istien Wardani ◽

Diana Soesilo

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Control Group ◽

Oral Diseases ◽

Clear Zone ◽

Control Group Design

Background: Dental and oral diseases which are often found in children is dental caries. Streptococcus mutans is the main cause of caries. Caries can be prevented by using a topical application of fluoride. The Anchovy (Stolephorus insularis) contains protein, vitamins (A, B1, C), and minerals (Fe, Ca, K, F). Calcium fluoride (CaF2) within the anchovy can inhibit the occurrence of dental caries. Purpose: The aim of this study was to determine the antimicrobial ability of anchovy extract (Stolephorus insularis) to Streptococcus mutans. Materials and Methods: This study was a laboratory experimental research with post test only control group design. Diffusion method were applied with 2 controls: negative control used DMSO 1%, positive control used NaF solution, and 3 concentrations of anchovy extract (Stolephorus insularis) 3%, 6%, and 12%, each group were composed of 6 samples. Antimicrobial was assessed by measuring the diameter of the clear zone around the discs contained the anchovy extract (Stolephorus insularis). Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney test. Result: The results from this study showed clear zone around the discs of the anchovy extract (Stolephorus insularis). The more concentration of the extract showed the more antimirobial zone diameter. The average zone of antimicrobial at the concentration of 3% were 7,11 mm, 6% 9,5 mm, 12% 10,78 mm, for the negative control DMSO 1% 6 mm and the positive control NaF solution 8,16 mm. The largest diameter of the clear zone was at concentration of 12% (P < 0,05). Conclusion: The anchovy extract (Stolephorus insularis) had an antimicrobial effect to the growth of Streptococcus mutans.

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ISOLASI LIKOPEN DARI BUAH TOMAT (Solanum lycopersicum L) dan UJI AKTIVITAS LIKOPEN TERHADAP BAKTERI Salmonella thypi

Jurnal Penelitian Pendidikan IPA ◽

10.29303/jppipa.v5i1.172 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Earlyna Sinthia Dewi ◽

Aliefman Hakim ◽

Lalu Rudyat Telly Savalas

Keyword(s):

Fourier Transform ◽

Wave Length ◽

Extraction Process ◽

Positive Control ◽

Negative Control ◽

Bacterial Activity ◽

Inhibitory Zone ◽

Solanum Lycopersicum L ◽

Mean Diameter ◽

The Mean

This research aims to know the effectiveness of lycopene antibacterial, through knowing bending zone lycopene towards salmonella thypi. This research has been success to isolate lycopene through extraction process used reflux method at 60oC temperature, using chloroform and methanol as antisolvent. Lycopene extract got 5,12 mg/100 g, analysis of functional groups by using spectroscopy Fourier Transform InfraRed (FTIR) detected C=C alkena alifatik at 1674,91 dan 1639,65 cm-1 wave length, C-H(CH3) detected at 1378,71 cm-1 wavelength, C-H (stretching) alifatikfunctional groups detected at 2853,12 cm-1 wave length, C-H alkena (stretching) detected at 2924,16 cm-1 wave length, and C-H alkena (bending) detected at 1498,86 cm-1 wave length. Inhibitory zone testing of salmonella thypi used paperdisc method with three repetitions. The mean diameter of the inhibitory zone of bacteria formed in positive control was 33,17 mm, negative control was 0 mm. Mean diameter of bacterial inhibitory zone at 3 % concentration with 3, 68 mm mean diameter and maximal Inhibitory zone at 50 % concentration with 15,12 mm mean diameter . The result of this research shown that lycopene extract has bacterial activity towards salmonella thypi Kewords: Isolation, Lycopene, Salmonella thypi, Antibacterial

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Penggunaan Kombinasi Ekstrak Akar Saluang Belum (Lavanga sarmentosa) dengan Akar Kuning (Arcangelisia flava Merr) terhadap Daya Hambat Bakteri Aeromonas hydrophila Secara In Vitro

Jurnal Ilmiah Universitas Batanghari Jambi ◽

10.33087/jiubj.v20i3.1015 ◽

2020 ◽

Vol 20 (3) ◽

pp. 1038

Author(s):

Maryani Maryani ◽

Kartika Bungas ◽

Hasan Anwar

Keyword(s):

Water Treatment ◽

Aeromonas Hydrophila ◽

Negative Control ◽

Distilled Water ◽

Clear Zone ◽

Water Handling ◽

Randomized Design ◽

Completely Randomized Design ◽

Inhibitory Power

Saluang Belum Root (Lavanga sarmentosa) and Yellow Root ((Arcangelisia flava) Merr) plants that have antibacterial effects need to be proven, for this research this program uses Saluang Belum Root (Lavanga sarmentosa) with Yellow Root (Arcangelisia flava) Merr) against bacterial inhibition. Aeromonas hydrophila by In Vitro. The method used in this study was a Completely Randomized Design (CRD) with 6 (six) settings and 3 (three) replications related to the aid of A = 25 gr / 150 ml distilled water, treatment B = 35 gr / 150 ml distilled water, C treatment = 45 gr / 150 ml distilled water, treatment D = 55 gr / 150 ml distilled water, handling E = 500 mg chloromphenicol / 150 ml distilled water, and treatment F = negative control only using the use of 150 ml distilled water. The results of this study indicate a combination of Saluang Belum Root Root (Lavanga sarmentosa) with Yellow Root (Arcangelisia flava Merr) which is able to inhibit the growth of My Aeromonas hydrophila bacteria because it has an antibacterial effect, this can be seen from the results of the zone average (inhibitory power) on each The treatment is the highest clear zone according to the D 55 gr / 150 ml treatment with an average clear zone of 15 mm and the lowest clear zone yield is A 25 gr / 150 ml approval with an average clear zone value of 11 mm. Keywords: Aeromonas hydrophila; Root of Saluang Not yet; Yellow Root; Antibacterial.

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Application of Bacillus probiotic to prevent Aeromonas hydrophilla infection in Clarias sp.

Jurnal Akuakultur Indonesia ◽

10.19027/jai.13.105-114 ◽

2015 ◽

Vol 13 (2) ◽

pp. 105 ◽

Cited By ~ 3

Author(s):

Mohammad Faizal Ulkhaq ◽

, Widanarni ◽

Angela Mariana Kusumastuti

Keyword(s):

Aeromonas Hydrophila ◽

Pathogenic Bacteria ◽

Clarias Gariepinus ◽

Positive Control ◽

Negative Control ◽

Probiotic Bacteria ◽

African Catfish ◽

Control Application

ABSTRACT The aim of this study was to test the effectiveness of a probiotic Bacillus for the prevention of motile aeromonad septicemia (MAS) disease caused by Aeromonas hydrophila in African catfish (Clarias gariepinus). The study consisted of the inhibition testing of A. hydrophila by Bacillus (in vitro) and the application of probiotic in African catfish (in vivo). The in vivo test, consisted of five treatments such as the addition of probiotic Bacillus P4I1 RifR, Bacillus P4I2 RifR, Bacillus P4I1 RifR + Bacillus P4I2 RifR (Kom), positive control (K+; only added with A. hydrophila) and negative control (K-; without probiotic nor A. hydrophila addition). African catfish (13.35±2.80 g) was maintained in 15 aquariums (40 L in volume) with 30 fishes each for 30 days. Probiotic bacteria was applied in water once a day, whereas pathogenic bacteria A. hydrophila RifR (103 cfu/mL) were added once in earlier treatment (except for the negative control). The result showed that the optimal concentration of Bacillus to inhibit A. hydrophila on in vitro test was 104 cfu/mL. In vivo test showed that the addition of probiotic in media of cultivation could reduce the number of A. hydrophila, improve immune response, and also increase the survival of African catfish compared to positive control. Application of probiotic P4I1 RifR showed the highest survival (92.23%) of all treatments. Keywords: Bacillus, Clarias gariepinus, motile aeromonad septicemia, probiotic ABSTRAK Penelitian ini bertujuan untuk menguji efektivitas probiotik Bacillus dalam pencegahan penyakit motile aeromonad septicaemia (MAS) yang disebabkan oleh Aeromonas hydrophila pada ikan lele dumbo (Clarias gariepinus). Penelitian terdiri atas pengujian penghambatan bakteri probiotik Bacillus terhadap A. hydrophila secara in vitro, dilanjutkan dengan aplikasi pada budidaya ikan lele dumbo (in vivo). Pada uji in vivo, penelitian terdiri atas lima perlakuan yaitu budidaya ikan lele dumbo dengan penambahan probiotik Bacillus P4I1 RifR, Bacillus P4I2 RifR, kombinasi probiotik Bacillus P4I1 RifR + Bacillus P4I2 RifR (Kom), kontrol positif (K+; hanya ditambahkan A. hydrophila) dan kontrol negatif (K-; tanpa pemberian probiotik dan A. hydrophila). Ikan lele dumbo (13,35±2,80 g) dipelihara pada akuarium volume 40 L dengan kepadatan 30 ekor/akuarium selama 30 hari. Bakteri probiotik ditambahkan pada media pemeliharaan ikan setiap hari, sedangkan bakteri patogen A. hydrophila RifR (103 cfu/ mL) diberikan sekali pada awal pemeliharaan (kecuali pada kontrol negatif). Hasil penelitian menunjukkan bahwa konsentrasi terbaik pada penghambatan in vitro adalah dengan penambahan Bacillus 104 cfu/mL. Hasil uji in vivo menunjukkan perlakuan penambahan probiotik pada media budidaya efektif dapat menekan jumlah bakteri A. hydrophila, memperbaiki respons imun, dan meningkatkan kelangsungan hidup ikan lele dumbo dibanding kontrol positif. Perlakuan probiotik P4I1 RifR memberikan hasil terbaik dengan tingkat kelangsungan hidup tertinggi yaitu 92,23%. Kata kunci: Bacillus, Clarias gariepinus, motile aeromonad septicemia, probiotik

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Pengaruh Pemberian Sari Kecambah Kacang Hijau terhadap Kadar Malondialdehid pada Ikan Lele Dumbo (Clarias gariepinus) yang Terinfeksi Bakteri Aeromonas hydrophila [Effect of Mung Bean Sprouts Essences against Malondialdehyde Levels In African catfish (Clarias gariepinus) Infected by Bacteria Aeromonas hydrophila ]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v6i2.11302 ◽

2019 ◽

Vol 6 (2) ◽

pp. 155

Author(s):

Widy Ayunanti, Hari Suprapto, Boedi Setya Rahardja

Keyword(s):

Lipid Peroxidation ◽

Free Radicals ◽

Vitamin E ◽

Aeromonas Hydrophila ◽

Clarias Gariepinus ◽

Positive Control ◽

Negative Control ◽

Control Treatment ◽

African Catfish ◽

Bacterial Disease

Abstract African catfish (Clarias gariepinus) is a type of freshwater fish consumption with elongated body and smooth skin. Bacterial disease that often strikes African catfish and can cause death as well as a cause of primary infection is A.hydrophila. Bacterial infection may be one cause of the synthesis of lipid peroxidation which is an indication of the emergence of free radicals. And the end result of lipid peroxidation is malondialdehyde. Sprouts are the new little plants grow from seeds planted beans. Green bean seed germination will enrich vitamins including vitamin E, green beans or α-tocopherol. Vitamin E in maintaining the stability of the plasma membrane and protects the cell structure against damage caused by free radicals is by blocking the reaction initiation and propagation of lipid peroxidation reactions interrupt. Data analyze of this research was used Varian Analyze (ANAVA) and to found the lowest level was used Duncan Test. The average yield of malondialdehyde levels in the negative control treatment is 0.0386 ng / 100 mg mass, positive control (1 ml Aeromonas hydrophila 105 cfu per 5 liter) is 0.0962 ng / 100 mg mass, sprout extract treatment (0 , 1 ml per fish) is 0.05 ng / 100 mg mass, germination and pollen treatment Aeromonas hydrophila is 0.0626 ng / 100 mg mass. From these results indicate that the highest levels found in the positive control treatment while the lowest levels found in the negative control. These results also suggest that decreased levels of malondialdehyde using pollen germination is very significantly different with P < 0,01.

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Compelling palatability of flavoring Atractus AQVA® for Nile tilapia juveniles

Latin American Journal of Aquatic Research ◽

10.3856/vol48-issue2-fulltext-2355 ◽

2020 ◽

Vol 48 (2) ◽

pp. 323-328

Author(s):

Denis Rogério Sanches Alves ◽

Suzana Raquel De Oliveira ◽

Bruno Dos Santos Sosa ◽

Wilson Rogério Boscolo ◽

Altevir Signor ◽

...

Keyword(s):

Oreochromis Niloticus ◽

Nile Tilapia ◽

Crude Protein ◽

Control Diet ◽

Digital Camera ◽

Positive Control ◽

Negative Control ◽

Fold Reduction

This study determined the compelling palatability of Atractus AQVA® flavoring for Nile tilapia juveniles (Oreochromis niloticus). Five isoproteic (40% crude protein) and isoenergetic (3,420 kcal kg-1) experimental diets were elaborated containing 0.25 (A25), 0.50 (A50) and 0.75% (A75) flavoring inclusion, a positive control diet with 10% fishmeal (FPE) and a negative control without fishmeal (SPE). Five juvenile individuals (2.58 ± 0.27 g) were distributed in five 10 L tanks and were fed four times a day with one of the diets, randomly raffled. The same number of pellets was offered, and the following behaviors were observed: time to capture the first pellet, number of pellet rejections, and number of approximations without capture and consumed pellets in each feeding event, using three-minute recordings with a digital camera. A significant effect (P < 0.05) was found regarding the number of approximations without capturing the pellet, as well as a higher palatability index for A75, followed by A50, A25, SPE and FPE. Therefore, it was concluded that A75 provided the highest compelling palatability of all diets by increasing the palatability index by 10.49% and displaying a 23.13% reduced rejection of pellets, besides presenting a 3.3 fold reduction in the number of approximations without capturing pellets with diet FPE.

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