scholarly journals Peran Chitosan sebagai Pemacu Pertumbuhan Kultur Anggrek Dendrobium lasianthera J.J.Sm. secara in vitro

2020 ◽  
Vol 12 (1) ◽  
pp. 43-49
Author(s):  
Verena Agustini ◽  
Irma Rahayu ◽  
Leonardo A. Numberi ◽  
Ziyadatun Ni’mah
Keyword(s):  
Positive Influence ◽  
Leaf Length ◽  
Maximum Effect ◽  
Observation Data ◽  
Chitosan Concentration ◽  
Randomized Design ◽  
The Media ◽  
Number Of Leaves ◽  
Completely Randomized Design

The effect of chitosan  on micropropagation of Dendrobium lasianthera J.J.Sm was observed. The media used was Vacin and Went (VW) modified with the addition of coconut water and given chitosan with several concentrations. The study was conducted at the Plant Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University for 6 months, from April to September 2019. Experiments using a Completely Randomized Design (CRD) of 8 treatments with 3 explants in each bottle for total 3 bottle as a replication. The total explants used in this experiment was 72 explants. The concentration of chitosan were 0% , 1%, 5%, 10%, 15%, 20%, 40% and 50% separately. Observation data were analyzed using ANOVA, if there were significant differences followed by DMRT at the 5% level. Observation variables included number and length of roots, number and length of leaves. The results showed that giving chitosan 10% had a maximum effect on the number of roots and number of leaves, 6.67 and 4.67. As for the highest leaf length of 17.43 mm  and the longest root length 19.21 mm were in the media with the addition of chitosan concentration of 15%. The observations showed that chitosan  had a positive influence on the growth and development of orchids D. lasianthera plantlet  in vitro.Keywords : Chitosan, orchid, Vacin and Went, plantlet.

scholarly journals In vitro development of Encyclea cordigera in different concentrations of honey

2015 ◽  
Vol 46 (4) ◽  
pp. 590-592 ◽  
Author(s):  
Cibele Mantovani ◽  
Kathia Fernandes Lopes Pivetta
Keyword(s):  
Culture Media ◽  
Leaf Length ◽  
In Vitro Development ◽  
Randomized Design ◽  
Sucrose Medium ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Set Up ◽  
Vitro Development

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.

scholarly journals KOMBINASI MEDIA TANAM DAN PEMBERIAN BERBAGAI DOSIS PUPUK GROW QUICK LB TERHADAP PERTUMBUHAN ANGGREK DENDROBIUM (Dendrobium sp.) PASCA AKLIMATISASI

1930 ◽  
Vol 8 (01) ◽  
pp. 91-97
Author(s):  
Nofripa Herlina ◽  
Novia Gesriantuti ◽  
Anis Restiawati
Keyword(s):  
Economic Value ◽  
Leaf Length ◽  
Leaf Width ◽  
Growing Media ◽  
Randomized Design ◽  
Two Factors ◽  
The Media ◽  
Number Of Leaves ◽  
Medium Type

Dendrobium orchid is one of the very popular ornamental plant because of the flower is beautiful and has a high economic value. An effort to prevent the orchid from extinetion is by means of in vitro propagation. The study aims to look at the effect of various doses of fertilizers Grow Quick LB and types of growing media and their interaction on the growth of Dendrobium sp. post acclimatization.The completely randomized design was used with two factors. The firs factor was type medium (charcoal+fern, charcoal+cocopeat), the second factor was type of Grow Quick LB fertilizer (0ml/L, 1ml/L, 2ml/L, 3ml/L). Parameters observed were, plantlet height, leaf length, leaf width, and number of leaves. The results showed that the dosing of Grow Quick LB fertilizer 1ml/L showed the highest values for the parameters width of leaves and number of leaves, whereas a dose of 2ml/L showed the highest values for the parameters plantlet height, leaf length. A combination of types of growing media charcoal+cocopeat showed a better effect than the media charcoal+fern on all parameters. The interaction of medium type and Grow Quick LB fertilizer significantly different with plantlet height and leaf length and not significantly different with parameters leaf width, and number of leaves.

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

scholarly journals Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya
Keyword(s):  
Tissue Culture ◽  
Drought Tolerance ◽  
In Vitro Selection ◽  
Solanum Melongena ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

scholarly journals THE GROWTH OF KIWI SHOOT (ACTINIDIA DELICIOSA) ON VARIOUS KINDS OF GELLING AGENTS

2019 ◽  
Vol 5 (2) ◽  
pp. 67
Author(s):  
Mardiana Mardiana ◽  
Zainuddin Zainuddin ◽  
Mahfudz Mahfudz ◽  
Hawalina Hawalina
Keyword(s):  
Shoot Growth ◽  
Good Condition ◽  
Kiwi Fruit ◽  
Physiological Maturity ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Time Required ◽  
Number Of Shoots

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.

scholarly journals Utilização de diferentes substratos na propagação de Pyrostegia venusta através de estacas

2015 ◽  
Vol 10 (4) ◽  
pp. 38
Author(s):  
J. G. Crispim ◽  
M. M. Rêgo ◽  
E. R. Rêgo ◽  
Â. M. S. Pessoa ◽  
P. A. Barroso
Keyword(s):  
Analysis Of Variance ◽  
Leaf Length ◽  
Cattle Manure ◽  
Main Root ◽  
Plastic Tube ◽  
Tukey Test ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Pyrostegia Venusta

<p>Este trabalho teve por objetivo avaliar o desenvolvimento de clones de <em>Pyrostegia venusta </em>em diferentes tipos de substratos. O experimento foi conduzido em delineamento inteiramente casualizado, com 06 tratamentos e 10 repetições, cada repetição foi constituída por um tubete de polietileno com capacidade de 103cm<sup>3</sup>. Os tratamentos testados foram: (T1) 100% de areia lavada; (T2) 50% areia lavada + 50% substrato comercial Plantmax®; (T3) 100% de substrato comercial Plantmax®; (T4) 50% areia lavada + 50% de esterco bovino; (T5) 1/3 areia lavada + 1/3 esterco bovino +1/3 de Plantmax® e (T6) 50% esterco bovino + 50% de Areia lavada. Após 30 dias foram avaliados o número de estacas sobreviventes, comprimento do broto (cm), número de folhas, comprimento da folha (cm), número de raízes e comprimento da raiz principal (cm).  Os dados foram submetidos a análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Por meio da análise de variância (p&lt;0,01), detectou-se significância estatística, para as variáveis, sobrevivência das estacas e número de brotos por estaca de <em>P. venusta</em>, quando cultivadas em substrato composto por 50% areia lavada + 50% substrato comercial Plantmax®. Essa mistura apresenta a vantagem de ser mais econômica quando comparado ao substrato comercial Plantmax®, sendo portanto, recomendada na produção de mudas de <em>cipó-de-São João</em><em>.</em></p><p align="center"><strong><em>Utilization of different substrates in the propagation of Pyrostegia venusta by cuttings</em></strong></p><p><strong>Abstract</strong><strong>: </strong>The experiment was carried out in a completely randomized design with 06 treatments and 10 replicates, each replicate consisted of polyethylene plastic tube the size of 103cm<sup>3</sup>. The treatments were: (T1) 100% washed sand; (T2) 50% washed sand + 50% commercial substrate Plantmax®; (T3) 100% substrate commercial Plantmax®; (T4) 50 % washed sand + 50% cattle manure; (T5) 1/3 washed sand + 1/3 manure +1/3 Plantmax® and Plantmax (T6) 50 % cattle manure + 50% washed sand. After 30 days, were evaluated number of cutting survival, bud length in cm, number of leaves, leaf length in cm, number of roots and length of the main root. Data were submitted to analysis of variance and means were compared by Tukey test at 5% probability. By analysis of variance (p&lt; 0.01), there was significant differences for the variables, czutting survival and number of shoots per cutting when cultured on substrate composed of 50% washed sand + 50% commercial substrate Plantmax®. Furthermore, this mixture has the advantage to be cheaper as compared to substrate Plantmax®, so we recommend the seedlings of Saint John’s vine.</p><p><em><br /></em></p>

scholarly journals Pengaruh Campuran Pasir dan Serat Aren pada Kailan (Brassica oleraceae) dengan Hidroponik Substrat

2014 ◽  
Vol 16 (1) ◽  
pp. 19
Author(s):  
Samanhudi Samanhudi ◽  
Dwi Hardjoko ◽  
Riandy Adhitya
Keyword(s):  
Wood Fiber ◽  
Dry Weight ◽  
Fresh Weight ◽  
Randomized Design ◽  
The Media ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Brassica Oleraceae ◽  
Media Mix ◽  
Leaf Greenness

<p>Wood fiber waste of arenga produced from starch palm industry. It is not handled properly so that could be potentially cause problems for the surrounding community. Wood fiber waste of arenga is potentially used as a medium for hydroponics. The waste can be utilized to get a new composition of hydroponic media. This study aims to determine the effect of a mixture of sand with arenga fiber waste. This research was carried out by using a mixture of sand and arenga wood fiber waste as a medium. This is an experimental study using a completely randomized design. This study aims to determine the effect of a mixture of sand and arenga fiber waste for kailan growth. The results showed that the mixture of sand and arenga wood fiber waste has no effect on kailan growth. The combination of mixtures of sand and arenga fiber waste showed no interaction. Observations on plants such as plant height, number of leaves, leaf greenness levels, the fresh weight of crop stover and stover dry weight of the plant, showed a row of the highest results in the media mix washed sand, washed sand and rice husk</p>

scholarly journals Respon Pertumbuhan Tanaman Gelombang Cinta Akibat Pemberian Pupuk Daun Petrovita di Pembibitan Lanjutan

2013 ◽  
Vol 13 (1) ◽  
pp. 10-18
Author(s):  
Djoko Eko Hadi Susilo ◽  
Sri Handayani Wahyuningsih
Keyword(s):  
Love Wave ◽  
Growth Response ◽  
Leaf Length ◽  
Stem Height ◽  
Efficient Treatment ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Leaf Fertilizer ◽  
Treatment Design

This study aims to determine the growth response of the love wave plant (Anthurium plowmanii) on Petrovita leaf fertilizer in an advanced nursery conducted in August - October 2011 in Panarung Village, Pahandut District, Palangka Raya City. Single treatment design (one factor) with 10 (ten) repetitions in the form of Petrovita leaf fertilizer (D) consisting of 5 concentration levels, namely: d1 = 0.5 ml l-1 (0.05%); d2 = 1.0 ml l-1 (0.10%); d3 = 1.5 ml l-1 (0.15%); d4 = 2.0 ml l-1 (0.20%); and d5 = 2.5 ml l-1 (0.25%) using a completely randomized design. Observations were made when the plants in the advanced nurseries had received treatment applications 4 times at intervals of 2 weeks. Observations were made on plant height (cm); stem height (cm); number of leaves (leaves); leaf length (cm); leaf width (cm); and leaf area (cm2). The results of this study indicate that Petrovita complete leaf fertilizer has a very significant effect on increasing the growth of love wave plants aged 8 MST in advanced nurseries. Petrovita fertilizer concentration of 0.2% or 2 ml l-1 (d4) is the best and most efficient treatment for love wave plants in advanced nurseries. It is recommended to increase the growth of love wave plants in advanced nurseries using Petrovita 0.2% concentration but tried with an interval of less than 2 weeks (for example once a week).

scholarly journals Effect of a Fungi Complex in Nine Ecotypes of Cenchrus purpureus (Schumach) Morrone

2021 ◽  
Vol 13 (12) ◽  
Author(s):  
Primavera Pérez-Ramos ◽  
Yuri Villegas-Aparicio ◽  
Rigoberto Castro-Rivera ◽  
Ernesto Castañeda-Hidalgo ◽  
Aarón Martínez-Gutiérrez ◽  
...  
Keyword(s):  
Differential Effect ◽  
Leaf Length ◽  
Growth And Yield ◽  
Total Biomass ◽  
Growth Strategies ◽  
Randomized Design ◽  
Fungal Consortium ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Four Levels

Objective: The objective of this study was to evaluate the response of different concentrations of a fungal consortium on the growth and yield of Cenchrus purpureus ecotypes under storm conditions.Design/Methodology/Approach: An experiment was established under a completely randomized design with a 9X2X4 factorial arrangement. The factors were 9 ecotypes of Cenchrus purpureus, two seasons of the year (Summer-Fall and Winter-Spring), and four levels of mycorrhizal consortium. The variables evaluated were: number of buds, number of leaves, height of the bud, leaf length, total biomass, and leaf-stalk ratio.Results: The response of the ecotypes had a differential effect on the variables NB, LLL, TB and L/SR, while the season affected the variables LN, PH, LLL, MLL, SLL, TB and L/SR. Low inoculation levels increased the variables NB, PH, LLL, MLL and TB.Study Limitations/Implications: The evaluated factors indicate that the level of inoculant and the season determine the growth and yield of Cenchrus purpureus.Findings/Conclusions: The mycorrhizal consortium dose and season of the year mainly determined the growth and yield of C. purpureus. Ecotypes respond differently to changes in the season and in mycorrhizal consortium dose. The evaluated factors indicate that the mycorrhiza dose and the season of the year determine the growth and yield of C.purpureus. Two growth strategies of the ecotypes are visualized: 1) many buds, with few small leaves and 2) few buds with many large leaves

scholarly journals Growth Induction of Cavendish Buds (Musa Acuminata L.) on Difference Concentration of IBA and BA in Vitro

2019 ◽  
Vol 8 (2) ◽  
pp. 178-187
Author(s):  
Subandi M ◽  
Arkhan Jannata ◽  
Sofiya Hasani
Keyword(s):  
Musa Acuminata ◽  
Laboratory Research ◽  
Independent Effect ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growth Induction

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.

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