Short Term Effects on Bone Quality Associated with Consumption of Soy Protein Isolate and Other Dietary Protein Sources in Rapidly Growing Female Rats

2008 ◽  
Vol 233 (11) ◽  
pp. 1348-1358 ◽  
Author(s):  
Jin-Ran Chen ◽  
Rohit Singhal ◽  
Oxana P. Lazarenko ◽  
Xiaoli Liu ◽  
William R. Hogue ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Quality ◽  
Soy Protein ◽  
Dietary Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Female Rats ◽  
Protein Sources ◽  
Positive Effects ◽  
Ovx Rats

Beneficial effects of soy protein consumption on bone quality have been reported. The effects of other dietary protein sources such as whey protein hydrolysate (WPH) and rice protein isolate (RPI) on bone growth have been less well examined. The current study compared effects of feeding soy protein isolate (SPI), WPH and RPI for 14 d on tibial bone mineral density (BMD) and bone mineral content (BMC) in intact and ovariectomized (OVX) rapidly growing female rats relative to animals fed casein (CAS). The effects of estrogenic status on responses to SPI were also explored. Tibial peripheral quantitative computerized tomography (pQCT) showed all three protein sources had positive effects on either BMD or BMC relative to CAS ( P < 0.05), but SPI had greater effects in both intact and OVX female rats. SPI and E2 had positive effects on BMD and BMC in OVX rats ( P < 0.05). However, trabecular BMD was lower in a SPI + E2 group compared to a CAS + E2 group. In OVX rats, SPI increased serum bone formation markers, and serum from SPI-fed rats stimulated osteoblastogenesis in ex vivo. SPI also suppressed the bone resorption marker RatLaps ( P < 0.05). Both SPI and E2 increased alkaline phosphatase gene expression in bone, but only SPI decreased receptor activator of nuclear factor-κB ligand (RANKL) and estrogen receptor gene expression ( P < 0.05). These data suggest beneficial bone effects of a soy diet in rapidly growing animals and the potential for early soy consumption to increase peak bone mass.

scholarly journals Uterine physiological responses and global gene expression in ovariectomized (OVX) rats treated with soy protein isolate (SPI) or 17‐βestradiol

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Martin J Ronis ◽  
Michael Blackburn ◽  
Kartik Shankar ◽  
Horatio Gomez-Acevedo ◽  
Rohit Singhal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Physiological Responses ◽  
Soy Protein Isolate ◽  
Global Gene Expression ◽  
Protein Isolate ◽  
Ovx Rats

scholarly journals Dietary Soy Protein Isolate Attenuates Intestinal Immunoglobulin and Mucin Expression in Young Mice Compared with Casein

Nutrients ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2739
Author(s):  
Bin Zeng ◽  
Dongyang Wang ◽  
Hailong Wang ◽  
Ting Chen ◽  
Junyi Luo ◽  
...  
Keyword(s):  
Soy Protein ◽  
Dietary Protein ◽  
Transforming Growth Factor ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Janus Kinase ◽  
Mucin Production ◽  
Protein Sources ◽  
Gut Barrier Function ◽  
Mucin Expression

Dietary protein sources have profound effects on children and young animals, and are important for the gut barrier function and immune resilience. Milk and soy are the main sources of protein for children and young animals after weaning. The objective of this study was to compare the effects of dairy and soy proteins on the intestinal barrier in early development. Weanling C57BL/6 mice were fed AIN-93G diets prepared with casein or soy protein isolate (SPI) for 21 days. Compared with those fed with the casein diet, mice fed with the SPI diet did not change their body weight and organ coefficients, but increased their feed intake and ratio of feed to gain. SPI lowered the level of luminal secretory immunoglobulin A (SIgA) and downregulated the levels of IL-4, IL-13, polymeric immunoglobulin receptor (Pigr), Janus kinase 1 (Jak1), signal transducer and activator of transcription 6 (Stat6), and transforming growth factor-β (Tgfb) in the mouse ileum. Western blotting of ileal proteins confirmed that SPI suppressed the activation of the JAK1/STAT6 signaling pathway. Furthermore, SPI attenuated intestinal mucin production, as demonstrated by the decreased numbers of intestinal goblet cells and the reduced relative expression levels of mucin 1 (Muc1), mucin 2 (Muc2), trefoil factor 3 (Tff3), glucose-regulated protein 94 (Grp94), and anterior gradient homolog 2 (Agr2). The results indicated that the SPI diet could attenuate mouse intestinal immunity, as demonstrated by decreased SIgA and mucin production in the intestine. Therefore, we suggest that our findings should be of consideration when SPI or casein are used as dietary protein sources.

scholarly journals Mammary Gland Morphology and Gene Expression Differ in Female Rats Treated with 17β-Estradiol or Fed Soy Protein Isolate

Endocrinology ◽  
2012 ◽  
Vol 153 (12) ◽  
pp. 6021-6032 ◽  
Author(s):  
Martin J. J. Ronis ◽  
Kartik Shankar ◽  
Horacio Gomez-Acevedo ◽  
Leah Hennings ◽  
Rohit Singhal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Female Rats ◽  
17Β Estradiol ◽  
Mammary Gland Morphology

scholarly journals Effects of Short and Long-Term Soy Protein Isolate Intake on Hepatic Cytochrome P450 Expression in Obese Zucker Rats

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1225-1225
Author(s):  
Melisa Kozaczek ◽  
Walter Bottje ◽  
Reza Hakkak
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Global Gene Expression ◽  
Protein Isolate ◽  
Zucker Rats ◽  
Z Score ◽  
Short And Long Term ◽  
Obese Zucker Rats

Abstract Objectives To determine the effects feeding for 8 (short-term) and 16 weeks (long-term) soy protein isolate on hepatic CYP gene expression. Methods 7-weeks old rats were randomly assigned to either a casein (CAS) or a soy protein isolate (SPI) diet. They were provided the diets ad libitum for 8 and 16 weeks. Rats were euthanized and livers were stored at − 80°C. RNA was extracted from liver samples, and sequenced to obtain transcriptomic data (RNAseq). Ingenuity Pathway Analysis software (IPA, Qiagen, CA) was used in the analysis of global gene expression data. This analysis includes predictions of activation or inhibition of molecules or upstream regulators and functions based on a generated z-score and p-value of overlap (P = 0.05). Z-scores were consider significant when &gt; 2 (activation) and &lt; −2 (inhibition). Results Comparing short- vs long-term feeding revealed an increase in the number of down-regulated CYP genes from only 3 at 8 weeks of SPI diet to 10 at 16 weeks of same diet (P &lt; 0.05). In contrast, upregulated CYP gene numbers showed a small increase in long-term SPI diet compared to short-term, from 14 genes at 8 weeks to 17 genes at 16 weeks (P &lt; 0.05). In addition, we present a predicted activation of the transcription factor Aryl hydrocarbon receptor (AHR, activation z-score = 2.146, P = 4.20E-11), linked to the subsequent activation or up-regulation of various CYPs genes, indirectly leading to the activation and inhibition of two main metabolic functions under SPI feeding: conversion of lipid (lipid metabolism) –predicted to be activated (z-score = 2.089, P = 2.77E-08), and recruitment of phagocytes (inflammatory response) –predicted to be inhibited (z-score = −2.311, P = 2.10E-05). Conclusions Through global gene expression analysis we showed that gene expression of drug-metabolizing cytochrome P450 genes was modified in genetically obese Zucker rats after being fed a soy-based diet for short- and long-term, and that this change could have an important role in attenuation of liver steatosis. Further research is needed to corroborate these results. Funding Sources This study was supported in part by the College of Medicine's University Medical Group (RH) and the Arkansas Biosciences Institute (WB, RH).

Sign in / Sign up

Export Citation Format

Share Document