Consumption of Soy Protein Isolate Reduces Hepatic SREBP-1c and Lipogenic Gene Expression in Wild-Type Mice, but Not in FXR-Deficient Mice

2011 ◽  
Vol 75 (9) ◽  
pp. 1702-1707 ◽  
Author(s):  
Tsutomu HASHIDUME ◽  
Takashi SASAKI ◽  
Jun INOUE ◽  
Ryuichiro SATO
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Wild Type ◽  
Lipogenic Gene ◽  
Lipogenic Gene Expression ◽  
Deficient Mice

scholarly journals Cell-Type–Specific, Ketohexokinase-Dependent Induction by Fructose of Lipogenic Gene Expression in Mouse Small Intestine

2020 ◽  
Vol 150 (7) ◽  
pp. 1722-1730 ◽  
Author(s):  
Arwa Al-Jawadi ◽  
Chirag R Patel ◽  
Reilly J Shiarella ◽  
Emmanuellie Romelus ◽  
Madelyn Auvinen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Metabolic Diseases ◽  
Free Access ◽  
Wild Type ◽  
Cell Type ◽  
Lipogenic Gene ◽  
Lipogenic Genes ◽  
Lipogenic Gene Expression ◽  
Small Intestinal

ABSTRACT Background High intakes of fructose are associated with metabolic diseases, including hypertriglyceridemia and intestinal tumor growth. Although small intestinal epithelia consist of many different cell types, express lipogenic genes, and convert dietary fructose to fatty acids, there is no information on the identity of the cell type(s) mediating this conversion and on the effects of fructose on lipogenic gene expression. Objectives We hypothesized that fructose regulates the intestinal expression of genes involved in lipid and apolipoprotein synthesis, that regulation depends on the fructose transporter solute carrier family 2 member a5 [Slc2a5 (glucose transporter 5)] and on ketohexokinase (Khk), and that regulation occurs only in enterocytes. Methods We compared lipogenic gene expression among different organs from wild-type adult male C57BL mice consuming a standard vivarium nonpurified diet. We then gavaged twice daily for 2.5 d fructose or glucose solutions (15%, 0.3 mL per mouse) into wild-type, Slc2a5-knockout (KO), and Khk-KO mice with free access to the nonpurified diet and determined expression of representative lipogenic genes. Finally, from mice fed the nonpurified diet, we made organoids highly enriched in enterocyte, goblet, Paneth, or stem cells and then incubated them overnight in 10 mM fructose or glucose. Results Most lipogenic genes were significantly expressed in the intestine relative to the kidney, liver, lung, and skeletal muscle. In vivo expression of Srebf1, Acaca, Fasn, Scd1, Dgat1, Gk, Apoa4, and Apob mRNA and of Scd1 protein increased (P < 0.05) by 3- to 20-fold in wild-type, but not in Slc2a5-KO and Khk-KO, mice gavaged with fructose. In vitro, Slc2a5- and Khk-dependent, fructose-induced increases, which ranged from 1.5- to 4-fold (P < 0.05), in mRNA concentrations of all these genes were observed only in organoids enriched in enterocytes. Conclusions Fructose specifically stimulates expression of mouse small intestinal genes for lipid and apolipoprotein synthesis. Secretory and stem cells seem incapable of transport- and metabolism-dependent lipogenesis, occurring only in absorptive enterocytes.

scholarly journals Clusterin deficiency induces lipid accumulation and tissue damage in kidney

2018 ◽  
Vol 237 (2) ◽  
pp. 175-191 ◽  
Author(s):  
Jung-Yoon Heo ◽  
Ji-Eun Kim ◽  
Yongwook Dan ◽  
Yong-Woon Kim ◽  
Jong-Yeon Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chronic Kidney Disease ◽  
Lipid Metabolism ◽  
Kidney Disease ◽  
Lipid Accumulation ◽  
Transforming Growth Factor ◽  
Lipid Levels ◽  
Lipogenic Gene ◽  
Lipogenic Gene Expression ◽  
Deficient Mice

Clusterin is a secretory glycoprotein that is involved in multiple physiopathological processes, including lipid metabolism. Previous studies have shown that clusterin prevents hepatic lipid accumulation via suppression of sterol regulatory element-binding protein (SREBP) 1. In this study, we examined the role of clusterin in renal lipid accumulation in clusterin-knockout mice and NRK52e tubular epithelial cells. Clusterin deficiency increased the expression of SREBP1 and its target genes and decreased malonyl-CoA decarboxylase protein levels in the kidney. Expression of the endocytic receptor, megalin, and scavenger receptor class A was increased in clusterin-deficient mice. Functional analysis of lipid metabolism also revealed that lipid uptake and triglyceride synthesis were increased and fatty acid oxidation was reduced, leading to increased lipid accumulation in clusterin-deficient mice. These phenomena were accompanied by mesangial expansion, fibrosis and increased urinary protein-to-creatinine ratio. High-fat feeding aggravated these clusterin deficiency-induced pathological changes. Clusterin knockdown in NRK52e cells increased lipogenic gene expression and lipid levels, whereas overexpression of clusterin by treatment with adenovirus or recombinant clusterin protein suppressed lipogenic gene expression and lipid levels. Transforming growth factor-beta 1 (TGFB1) expression increased in the kidney of clusterin-deficient mice and suppression of TGFB1 in NRK52e cells suppressed lipid accumulation. These results suggest that clusterin deficiency induces renal lipid accumulation by dysregulating the expression of lipid metabolism-related factors and TGFB1, thereby leading to chronic kidney disease. Hence, clusterin may serve as a therapeutic target for lipid-induced chronic kidney disease.

scholarly journals Effects of Short and Long-Term Soy Protein Isolate Intake on Hepatic Cytochrome P450 Expression in Obese Zucker Rats

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1225-1225
Author(s):  
Melisa Kozaczek ◽  
Walter Bottje ◽  
Reza Hakkak
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Global Gene Expression ◽  
Protein Isolate ◽  
Zucker Rats ◽  
Z Score ◽  
Short And Long Term ◽  
Obese Zucker Rats

Abstract Objectives To determine the effects feeding for 8 (short-term) and 16 weeks (long-term) soy protein isolate on hepatic CYP gene expression. Methods 7-weeks old rats were randomly assigned to either a casein (CAS) or a soy protein isolate (SPI) diet. They were provided the diets ad libitum for 8 and 16 weeks. Rats were euthanized and livers were stored at − 80°C. RNA was extracted from liver samples, and sequenced to obtain transcriptomic data (RNAseq). Ingenuity Pathway Analysis software (IPA, Qiagen, CA) was used in the analysis of global gene expression data. This analysis includes predictions of activation or inhibition of molecules or upstream regulators and functions based on a generated z-score and p-value of overlap (P = 0.05). Z-scores were consider significant when > 2 (activation) and < −2 (inhibition). Results Comparing short- vs long-term feeding revealed an increase in the number of down-regulated CYP genes from only 3 at 8 weeks of SPI diet to 10 at 16 weeks of same diet (P < 0.05). In contrast, upregulated CYP gene numbers showed a small increase in long-term SPI diet compared to short-term, from 14 genes at 8 weeks to 17 genes at 16 weeks (P < 0.05). In addition, we present a predicted activation of the transcription factor Aryl hydrocarbon receptor (AHR, activation z-score = 2.146, P = 4.20E-11), linked to the subsequent activation or up-regulation of various CYPs genes, indirectly leading to the activation and inhibition of two main metabolic functions under SPI feeding: conversion of lipid (lipid metabolism) –predicted to be activated (z-score = 2.089, P = 2.77E-08), and recruitment of phagocytes (inflammatory response) –predicted to be inhibited (z-score = −2.311, P = 2.10E-05). Conclusions Through global gene expression analysis we showed that gene expression of drug-metabolizing cytochrome P450 genes was modified in genetically obese Zucker rats after being fed a soy-based diet for short- and long-term, and that this change could have an important role in attenuation of liver steatosis. Further research is needed to corroborate these results. Funding Sources This study was supported in part by the College of Medicine's University Medical Group (RH) and the Arkansas Biosciences Institute (WB, RH).

Short Term Effects on Bone Quality Associated with Consumption of Soy Protein Isolate and Other Dietary Protein Sources in Rapidly Growing Female Rats

2008 ◽  
Vol 233 (11) ◽  
pp. 1348-1358 ◽  
Author(s):  
Jin-Ran Chen ◽  
Rohit Singhal ◽  
Oxana P. Lazarenko ◽  
Xiaoli Liu ◽  
William R. Hogue ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Quality ◽  
Soy Protein ◽  
Dietary Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Female Rats ◽  
Protein Sources ◽  
Positive Effects ◽  
Ovx Rats

Beneficial effects of soy protein consumption on bone quality have been reported. The effects of other dietary protein sources such as whey protein hydrolysate (WPH) and rice protein isolate (RPI) on bone growth have been less well examined. The current study compared effects of feeding soy protein isolate (SPI), WPH and RPI for 14 d on tibial bone mineral density (BMD) and bone mineral content (BMC) in intact and ovariectomized (OVX) rapidly growing female rats relative to animals fed casein (CAS). The effects of estrogenic status on responses to SPI were also explored. Tibial peripheral quantitative computerized tomography (pQCT) showed all three protein sources had positive effects on either BMD or BMC relative to CAS ( P < 0.05), but SPI had greater effects in both intact and OVX female rats. SPI and E2 had positive effects on BMD and BMC in OVX rats ( P < 0.05). However, trabecular BMD was lower in a SPI + E2 group compared to a CAS + E2 group. In OVX rats, SPI increased serum bone formation markers, and serum from SPI-fed rats stimulated osteoblastogenesis in ex vivo. SPI also suppressed the bone resorption marker RatLaps ( P < 0.05). Both SPI and E2 increased alkaline phosphatase gene expression in bone, but only SPI decreased receptor activator of nuclear factor-κB ligand (RANKL) and estrogen receptor gene expression ( P < 0.05). These data suggest beneficial bone effects of a soy diet in rapidly growing animals and the potential for early soy consumption to increase peak bone mass.

scholarly journals Uterine physiological responses and global gene expression in ovariectomized (OVX) rats treated with soy protein isolate (SPI) or 17‐βestradiol

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Martin J Ronis ◽  
Michael Blackburn ◽  
Kartik Shankar ◽  
Horatio Gomez-Acevedo ◽  
Rohit Singhal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Physiological Responses ◽  
Soy Protein Isolate ◽  
Global Gene Expression ◽  
Protein Isolate ◽  
Ovx Rats

Intake of Soy Protein Isolate Alters Hepatic Gene Expression in Rats

2005 ◽  
Vol 53 (10) ◽  
pp. 4253-4257 ◽  
Author(s):  
Nobuhiko Tachibana ◽  
Ichiro Matsumoto ◽  
Kensuke Fukui ◽  
Soichi Arai ◽  
Hisanori Kato ◽  
...  
Keyword(s):  
Gene Expression ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Hepatic Gene Expression

scholarly journals Mammary Gland Morphology and Gene Expression Differ in Female Rats Treated with 17β-Estradiol or Fed Soy Protein Isolate

Endocrinology ◽  
2012 ◽  
Vol 153 (12) ◽  
pp. 6021-6032 ◽  
Author(s):  
Martin J. J. Ronis ◽  
Kartik Shankar ◽  
Horacio Gomez-Acevedo ◽  
Leah Hennings ◽  
Rohit Singhal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Female Rats ◽  
17Β Estradiol ◽  
Mammary Gland Morphology
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