scholarly journals The probable cause for the failure of some sodium tungstate to give a suitable reagent for the determination of uric acid

1918 ◽  
Vol 16 (1) ◽  
pp. 10-11
Author(s):  
G. Egerer ◽  
F. Ford
Keyword(s):  
Uric Acid ◽  
Sodium Tungstate

Semimicro Method for Determination of Serum Uric Acid Using EDTA—Hydrazine

1968 ◽  
Vol 14 (8) ◽  
pp. 764-775 ◽  
Author(s):  
C P Patel
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Serum Uric Acid ◽  
Phosphotungstic Acid ◽  
Sodium Salicylate ◽  
Sodium Tungstate ◽  
Hydrazine Sulfate ◽  
Spectrophotometric Methods ◽  
Automated Procedures

Abstract A semimicro method is described for the determination of uric acid by manual and automated procedures. Serum uric acid reduces phosphotungstic acid in an alkaline medium of EDTA sodium tungstate and hydrazine sulfate, the latter being used as a color intensifier. The use of sodium tungstate eliminates the development of interfering turbidity encountered with other alkalizing reagents. This method requires only 0.2 ml. of serum. The presence of ascorbic acid, sodium salicylate, cysteine, and/or glucose does not interfere. A linear relationship is obtained upon comparing this method with the sodium carbonate and the differential ultraviolet spectrophotometric methods.

Deproteinizing methods evaluated for determination of uric acid in serum by reversed-phase liquid chromatography with ultraviolet detection.

1987 ◽  
Vol 33 (8) ◽  
pp. 1427-1430 ◽  
Author(s):  
R Sakuma ◽  
T Nishina ◽  
M Kitamura
Keyword(s):  
Uric Acid ◽  
Liquid Chromatography ◽  
Perchloric Acid ◽  
High Performance ◽  
Reversed Phase ◽  
Precipitation Method ◽  
Zinc Hydroxide ◽  
Good Precision ◽  
Ultraviolet Detection

Abstract We evaluated six deproteinizing methods for determination of uric acid in serum by "high-performance" liquid chromatography with ultraviolet detection: those involving zinc hydroxide, sodium tungstate, trichloroacetic acid, perchloric acid, acetonitrile, and centrifugal ultrafiltration (with Amicon MPS-1 devices). We used a Toyosoda ODS-120A reversed-phase column. The mobile phase was sodium phosphate buffer (40 mmol/L, pH 2.2) containing 20 mL of methanol per liter. Absorbance of the eluate was monitored at 284 nm. The precipitation method with perchloric acid gave high recoveries of uric acid and good precision, and results agreed with those by the uricase-catalase method of Kageyama (Clin Chim Acta 1971;31:421-6).

scholarly journals THE DETERMINATION OF URIC ACID IN THE STUDY OF AVIAN NUTRITION

1931 ◽  
Vol 92 (1) ◽  
pp. 41-45
Author(s):  
J.L. St. John ◽  
Otto Johnson
Keyword(s):  
Uric Acid

scholarly journals COLORIMETRIC DETERMINATION OF URIC ACID

1922 ◽  
Vol 50 (1) ◽  
pp. 55-63 ◽  
Author(s):  
J.Lucien Morris ◽  
A.Garrard Macleod
Keyword(s):  
Uric Acid ◽  
Colorimetric Determination

scholarly journals Simultaneous Determination of Uric Acid and Xanthine Using a Poly(Methylene Blue) and Electrochemically Reduced Graphene Oxide Composite Film Modified Electrode

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Gen Liu ◽  
Wei Ma ◽  
Yan Luo ◽  
Deng-ming Sun ◽  
Shuang Shao
Keyword(s):  
Methylene Blue ◽  
Uric Acid ◽  
Graphene Oxide ◽  
Composite Film ◽  
Simultaneous Determination ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

Poly(methylene blue) and electrochemically reduced graphene oxide composite film modified electrode (PMB-ERGO/GCE) was successfully fabricated by electropolymerization and was used for simultaneous determination of uric acid (UA) and xanthine (Xa). Based on the excellent electrocatalytic activity of PMB-ERGO/GCE, the electrochemical behaviors of UA and Xa were studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). Two anodic sensitive peaks at 0.630 V (versus Ag/AgCl) for UA and 1.006 V (versus Ag/AgCl) for Xa were given by CV in pH 3.0 phosphate buffer. The calibration curves for UA and Xa were obtained in the range of 8.00 × 10−8~4.00 × 10−4 M and 1.00 × 10−7~4.00 × 10−4 M, respectively, by SWV. The detection limits for UA and Xa were3.00×10-8 M and5.00×10-8 M, respectively. Finally, the proposed method was applied to simultaneously determine UA and Xa in human urine with good selectivity and high sensitivity.

Ultramicro Automated Screening Method for Uric Acid

1967 ◽  
Vol 13 (11) ◽  
pp. 985-993 ◽  
Author(s):  
Ronald H Laessig ◽  
Chester E Underwood ◽  
Barbara J Basteyns
Keyword(s):  
Uric Acid ◽  
Phosphotungstic Acid ◽  
Screening Method ◽  
Sampling Procedure ◽  
Blood Capillary ◽  
Acid Complex ◽  
Blood Uric Acid ◽  
Precision And Accuracy ◽  
Automated Screening

Abstract An automated colorimetric microprocedure, suitable for screening purposes, has been developed for the determination of blood uric acid levels. The method uses 2O-µl. whole-blood (capillary) samples and is based on the AutoAnalyzer measurement of the absorbance of the colored uric acid-phosphotungstic acid complex. The dilution inherent in the sampling procedure necessitated a modification of the existing AutoAnalyzer method to increase the sensitivity. The proposed method is evaluated for precision and accuracy by comparison with the standard AutoAnalyzer macro-method.

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