scholarly journals Within-herd prevalence thresholds for herd-level detection of mastitis pathogens using multiplex real-time PCR in bulk tank milk samples

2017 ◽  
Vol 100 (10) ◽  
pp. 8287-8295 ◽  
Author(s):  
J.B. Soltau ◽  
E. Einax ◽  
K. Klengel ◽  
J. Katholm ◽  
K. Failing ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bulk Tank Milk ◽  
Herd Level ◽  
Herd Prevalence ◽  
Milk Samples ◽  
Bulk Tank ◽  
Mastitis Pathogens

scholarly journals Rapid Detection of Mycoplasma bovis, Staphylococcus aureus and Streptococcus agalactiae in Cattle Bulk Tank Milk in Cyprus and Relations with Somatic Cell Counts

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 841
Author(s):  
Maria Liapi ◽  
George Botsaris ◽  
Costas Arsenoglou ◽  
Nikolas Markantonis ◽  
Christodoulos Michael ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Somatic Cell ◽  
Real Time ◽  
Streptococcus Agalactiae ◽  
Mycoplasma Bovis ◽  
Bulk Tank Milk ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
Cell Counts ◽  
Bulk Tank

One hundred and seventy-seven (177) bulk tank milk samples were analyzed with a commercially available real-time polymerase chain reaction kit and 11 (6.21%), 41 (23.16%), and 58 (32.77%) tested positive for Mycoplasma bovis, Staphylococcus aureus, and Streptococcus agalactiae, respectively. Statistical analysis revealed a significant relationship between the presence of S. aureus and S. agalactiae. Enumeration of somatic cells was performed in the same samples by flow cytometry. The somatic cell counts were found higher in S. aureus and S. agalactiae positive samples. No association was found between M. bovis presence and somatic cells counts. Low internal assay control Ct values were found to be related with high somatic cell counts. Noticeably, this is the first report for the presence of M. bovis in Cyprus. Therefore, its presence was confirmed by bulk tank milk culture, conventional PCR, and next generation sequencing. Furthermore, M. bovis was typed with multilocus sequencing typing and was allocated to sequence type 29 (ST 29). Real-time PCR in bulk tank milk samples is a useful tool to detect mammary infections, especially for neglected pathogens such as M. bovis.

scholarly journals Estimated herd prevalence and sequence types of Coxiella burnetii in bulk tank milk samples from commercial dairies in Indiana

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Amy E. Bauer ◽  
Sonora Olivas ◽  
Maria Cooper ◽  
Heidie Hornstra ◽  
Paul Keim ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Bulk Tank Milk ◽  
Herd Prevalence ◽  
Milk Samples ◽  
Bulk Tank ◽  
Sequence Types

Application of an F57 Sequence-Based Real-Time PCR Assay for Mycobacterium paratuberculosis Detection in Bulk Tank Raw Milk and Slaughtered Healthy Dairy Cows

2006 ◽  
Vol 69 (7) ◽  
pp. 1662-1667 ◽  
Author(s):  
C. BOSSHARD ◽  
R. STEPHAN ◽  
T. TASARA
Keyword(s):  
Detection Limit ◽  
Real Time ◽  
Real Time Pcr ◽  
Raw Milk ◽  
Mesenteric Lymph Nodes ◽  
Pcr Assay ◽  
Milk Samples ◽  
Light Cycler ◽  
Bulk Tank ◽  
Mycobacterium Avium Subsp Paratuberculosis

A light cycler–based real-time PCR assay that targets the F57 sequence was used to collect data on the prevalence of Mycobacterium avium subsp. paratuberculosis (MAP) in 100 bulk tank raw milk samples and in a population of 101 slaughtered dairy cattle. The assay's reproducible detection limit in total genomic DNA templates isolated from 10-ml samples of MAP-spiked raw milk was 100 cells per ml. Similarly, the evaluation of MAP-spiked bovine feces also demonstrated that the assay had a reproducible detection limit of 100 cells if they were contained within 200 mg of fecal sample material. Among the 100 bulk tank milk samples that were tested, we found 3 samples (3%) to be positive for MAP. In the slaughterhouse part of the study, 8.9% (9 of 101) of the cows were positive for MAP DNA in fecal samples, 4.9% (5 of 101) in mesenteric lymph nodes, 0.9% (1 of 101) in ileum tissue, and 3.6% (3 of 84) in milk. Meanwhile, for 2.9% (3 of 101) of the culled cows, MAP DNA was detected in samples of diaphragmatic muscles.

Prevalence of Salmonella enterica, Listeria monocytogenes, and Escherichia coli Virulence Factors in Bulk Tank Milk and In-Line Filters from U.S. Dairies†

2011 ◽  
Vol 74 (5) ◽  
pp. 759-768 ◽  
Author(s):  
JO ANN S. VAN KESSEL ◽  
JEFFREY S. KARNS ◽  
JASON E. LOMBARD ◽  
CHRISTINE A. KOPRAL
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Real Time ◽  
Real Time Pcr ◽  
Salmonella Enterica ◽  
Raw Milk ◽  
The United States ◽  
Bulk Tank Milk ◽  
Milk Products ◽  
Bulk Tank

The zoonotic bacteria Salmonella enterica, Listeria monocytogenes, and Escherichia coli are known to infect dairy cows while not always causing clinical signs of disease. These pathogens are sometimes found in raw milk, and human disease outbreaks due to these organisms have been associated with the consumption of raw milk or raw milk products. Bulk tank milk (BTM) samples (536) and in-line milk filters (519) collected from dairy farms across the United States during the National Animal Health Monitoring System's Dairy 2007 study were analyzed by real-time PCR for the presence of S. enterica and pathogenic forms of E. coli and by culture techniques for the presence of L. monocytogenes. S. enterica was detected in samples from 28.1% of the dairy operations, primarily in milk filters. Salmonella was isolated from 36 of 75 PCR-positive BTM samples and 105 of 174 PCR-positive filter samples, and the isolates were serotyped. Cerro, Kentucky, Muenster, Anatum, and Newport were the most common serotypes. L. monocytogenes was isolated from 7.1% of the dairy operations, and the 1/2a complex was the most common serotype, followed by 1/2b and 4b (lineage 1). Shiga toxin genes were detected in enrichments from 15.2% of the BTM samples and from 51.0% of the filters by real-time PCR. In most cases, the cycle threshold values for the PCR indicated that toxigenic strains were not a major part of the enrichment populations. These data confirm those from earlier studies showing significant contamination of BTM by zoonotic bacterial pathogens and that the consumption of raw milk and raw milk products presents a health risk.

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