scholarly journals Influence of conjugated linoleic acid and vitamin E on performance, energy metabolism, and change of fat depot mass in transitional dairy cows

2017 ◽  
Vol 100 (4) ◽  
pp. 3193-3208 ◽  
Author(s):  
S. Schäfers ◽  
D. von Soosten ◽  
U. Meyer ◽  
C. Drong ◽  
J. Frahm ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Vitamin E ◽  
Energy Metabolism ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Fat Depot

Effect of vitamin E on milk composition of grazing dairy cows supplemented with microencapsulated conjugated linoleic acid

2013 ◽  
Vol 45 (8) ◽  
pp. 1783-1788 ◽  
Author(s):  
Monica Ramírez-Mella ◽  
Omar Hernández-Mendo ◽  
Efren Jacinto Ramírez-Bribiesca ◽  
Ricardo Daniel Améndola-Massiotti ◽  
María M. Crosby-Galván ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Vitamin E ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Milk Composition ◽  
Grazing Dairy Cows

scholarly journals Phosphoproteomic Analysis of Subcutaneous and Omental Adipose Tissue Reveals Increased Lipid Turnover in Dairy Cows Supplemented with Conjugated Linoleic Acid

2021 ◽  
Vol 22 (6) ◽  
pp. 3227
Author(s):  
Jayasimha Rayalu Daddam ◽  
Harald M. Hammon ◽  
Arnulf Tröscher ◽  
Laura Vogel ◽  
Martina Gnott ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Fatty Acid Synthase ◽  
Control Diet ◽  
Endocannabinoid System ◽  
Hormone Sensitive Lipase ◽  
Phosphorylation Sites ◽  
Lipid Turnover

Phosphoproteomics is a cutting-edge technique that can be utilized to explore adipose tissue (AT) metabolism by quantifying the repertoire of phospho-peptides (PP) in AT. Dairy cows were supplemented with conjugated linoleic acid (CLA, n = 5) or a control diet (CON, n = 5) from 63 d prepartum to 63 d postpartum; cows were slaughtered at 63 d postpartum and AT was collected. We performed a quantitative phosphoproteomics analysis of subcutaneous (SC) and omental (OM) AT using nanoUPLC-MS/MS and examined the effects of CLA supplementation on the change in the phosphoproteome. A total of 5919 PP were detected in AT, and the abundance of 854 (14.4%) were differential between CON and CLA AT (p ≤ 0.05 and fold change ± 1.5). The abundance of 470 PP (7.9%) differed between OM and SC AT, and the interaction treatment vs. AT depot was significant for 205 PP (3.5% of total PP). The integrated phosphoproteome demonstrated the up- and downregulation of PP from proteins related to lipolysis and lipogenesis, and phosphorylation events in multiple pathways, including the regulation of lipolysis in adipocytes, mTOR signaling, insulin signaling, AMPK signaling, and glycolysis. The differential regulation of phosphosite on a serine residue (S777) of fatty acid synthase (FASN) in AT of CLA-supplemented cows was related to lipogenesis and with more phosphorylation sites compared to acetyl-coenzyme A synthetase (ACSS2). Increased protein phosphorylation was seen in acetyl-CoA carboxylase 1 (ACACA;8 PP), FASN (9 PP), hormone sensitive lipase (LIPE;6 PP), perilipin (PLIN;3 PP), and diacylglycerol lipase alpha (DAGLA;1 PP) in CLA vs. CON AT. The relative gene expression in the SC and OM AT revealed an increase in LIPE and FASN in CLA compared to CON AT. In addition, the expression of DAGLA, which is a lipid metabolism enzyme related to the endocannabinoid system, was 1.6-fold higher in CLA vs. CON AT, and the expression of the cannabinoid receptor CNR1 was reduced in CLA vs. CON AT. Immunoblots of SC and OM AT showed an increased abundance of FASN and a lower abundance of CB1 in CLA vs. CON. This study presents a complete map of the SC and the OM AT phosphoproteome in dairy cows following CLA supplementation and discloses many unknown phosphorylation sites, suggestive of increased lipid turnover in AT, for further functional investigation.

scholarly journals Influence of Dietary Fish Oil on Conjugated Linoleic Acid and Other Fatty Acids in Milk Fat from Lactating Dairy Cows

2000 ◽  
Vol 83 (11) ◽  
pp. 2620-2628 ◽  
Author(s):  
D.C. Donovan ◽  
D.J. Schingoethe ◽  
R.J. Baer ◽  
J. Ryali ◽  
A.R. Hippen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Fish Oil ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Lactating Dairy Cows ◽  
Dietary Fish

scholarly journals Conjugated Linoleic Acid Is Synthesized Endogenously in Lactating Dairy Cows by Δ9-Desaturase

2000 ◽  
Vol 130 (9) ◽  
pp. 2285-2291 ◽  
Author(s):  
J. M. Griinari ◽  
B. A. Corl ◽  
S. H. Lacy ◽  
P. Y. Chouinard ◽  
K. V. V. Nurmela ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Lactating Dairy Cows ◽  
Δ9 Desaturase

Lactation response of cows to different levels of ruminally inert conjugated linoleic acids under commercial conditions

2005 ◽  
Vol 85 (2) ◽  
pp. 231-242 ◽  
Author(s):  
Rachel Gervais ◽  
Richard Spratt ◽  
Martin Léonard ◽  
P. Yvan Chouinard
Keyword(s):  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Milk Production ◽  
Milk Fat ◽  
Metabolic Profile ◽  
Milk Composition ◽  
Dietary Lipids ◽  
Calcium Salts ◽  
Lactating Cows

Dietary conjugated linoleic acid (CLA) supplements have been shown to reduce milk fat synthesis in dairy cows. A rumen-inert source of CLA is required for commercial feed applications. The conversion of dietary lipids to a calcium salt is considered as a method to counter the extensive hydrogenation of dietary lipids that occurs in the rumen. Our objective was to determine whether feeding calcium salts of CLA under commercial conditions would affect milk production, milk composition and blood metabolic profile. A total of 240 dairy cows from eight farms were blocked according to the calving date, and randomly assigned to four treatments providing CLA at 0, 8, 16 and 32 g d-1. Milk production was recorded and milk was sampled on day 0, 7, 14, 28 and 42 of the feeding period. Blood samples were taken on day 42 from early-lactating cows (< 157 d in milk) to determine the metabolic profile. Milk fat yield was decreased 11, 20 and 28%, and milk fat concentration was reduced 13, 22 and 28% (linear; P < 0.001) when cows received 8, 16 and 32 g d-1 of CLA, respectively. Milk yield, milk protein and blood metabolic parameters were not affected by experimental treatments. Calcium salts of CLA can be used as an effective tool to manage milk fat content on commercial dairy farms. Key words: Conjugated linoleic acid, milk fat, ruminally inert fat

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