scholarly journals VISUALIZATION OF ACOUSTIC PROPERTIES OF BIOLOGICAL TISSUES BY SCANNING ACOUSTIC MICROSCOPY

1994 ◽  
Vol 14 (Supplement2) ◽  
pp. 189-192
Author(s):  
Yoshifumi SAIJO ◽  
Motonao TANAKA
2012 ◽  
Vol 729 ◽  
pp. 356-360
Author(s):  
Endre Harkai ◽  
Tamás Hurtony ◽  
Péter Gordon

Microhardness and sound velocity were measured in case of differently prepared solder samples. The used Pb-10Sn solder samples were melted then cooled down applying different cooling rates. These procedures caused variant microstructure thus different microhardness and sound velocity values. The sound velocity was measured by means of scanning acoustic microscopy. Characterization of solder materials by acoustic microscopy gives the possibility to non-destructively estimate mechanical and reliability parameters of the given material.


1998 ◽  
Vol 24 (9) ◽  
pp. 1397-1405 ◽  
Author(s):  
Aki Kinoshita ◽  
Shoichi Senda ◽  
Katsufumi Mizushige ◽  
Hisashi Masugata ◽  
Seiji Sakamoto ◽  
...  

2004 ◽  
Vol 08 (01) ◽  
pp. 29-34 ◽  
Author(s):  
Hirotaka Sano ◽  
Yoshifumi Saijo ◽  
Shoichi Kokubun

The acoustic properties of the supraspinatus tendon insertions of three adult Japanese white rabbits (male, 3.2–3.5 kg) were measured by scanning acoustic microscopy (SAM). After cutting parallel to the supraspinatus tendon fibers, specimens were fixed with 10% neutralized formalin, embedded in paraffin and sectioned at 5 μm thickness. The sound speed and the attenuation constant along the articular surface of the tendon from the insertion site to the musculotendinous junction were measured by SAM. The two-dimensional distribution of the acoustic properties was displayed with color-coded scales and was compared to the histologic structure. Toluidine-blue staining and immunohistochemical staining against type II collagen were performed to determine the presence of fibrocartilage besides a standard Elastica–Masson staining to assess the overall histologic structure. The distribution of the sound speed and the attenuation constant showed an almost identical pattern. Both parameters were lower in the non-mineralized fibrocartilage than those in the tendon proper. The area indicating low sound speed corresponded well to the area of metachromasia and type II collagen. The non-mineralized fibrocartilage indicated softer material properties than the tendon proper, which could be interpreted as an adaptation for the various biomechanical stresses at the insertion site.


1997 ◽  
Vol 12 (10) ◽  
pp. 2151-2154 ◽  
Author(s):  
H. Sasaki ◽  
Y. Saijo ◽  
M. Tanaka ◽  
S. Nitta ◽  
Y. Terasawa ◽  
...  

2011 ◽  
Vol 1301 ◽  
Author(s):  
Xuegen Zhao ◽  
Steven Wilkinson ◽  
Riaz Akhtar ◽  
Michael J Sherratt ◽  
Rachel E B Watson ◽  
...  

ABSTRACTIn this study we have established a new approach to more accurately map acoustic wave speed (which is a measure of stiffness) within soft biological tissues at micrometer length scales using scanning acoustic microscopy. By using thin (5 μm thick) histological sections of human skin and porcine cartilage, this method exploits the phase information preserved in the interference between acoustic waves reflected from the substrate surface as well as internal reflections from the acoustic lens. A stack of images were taken with the focus point of acoustic lens positioned at or above the substrate surface, and processed pixel by pixel using custom software developed with LABVIEW and IMAQ (National Instruments) to extract phase information. Scanning parameters, such as acoustic wave frequency and gate position were optimized to get reasonable phase and lateral resolution. The contribution from substrate inclination or uneven scanning surface was removed prior to further processing. The wave attenuation was also obtained from these images.


Ultrasonics ◽  
2004 ◽  
Vol 42 (1-9) ◽  
pp. 695-698 ◽  
Author(s):  
Yoshifumi Saijo ◽  
Toshifumi Miyakawa ◽  
Hidehiko Sasaki ◽  
Motonao Tanaka ◽  
Shin-ichi Nitta

2008 ◽  
Vol 1132 ◽  
Author(s):  
Riaz Akhtar ◽  
Michael J. Sherratt ◽  
Rachel E.B. Watson ◽  
Tribikram Kundu ◽  
Brian Derby

ABSTRACTAlthough the gross mechanical properties of ageing tissues have been extensively documented, biological tissues are highly heterogeneous and little is known concerning the variation of micro-mechanical properties within tissues. Here, we use Scanning Acoustic Microscopy (SAM) to map the acoustic wave speed (a measure of stiffness) as a function of distance from the outer adventitial layer of cryo-sectioned ferret aorta. With a 400 MHz lens, the images of the aorta samples matched those obtained following chemical fixation and staining of sections which were viewed with fluorescence microscopy. Quantitative analysis was conducted with a frequency scanning or V(f) technique by imaging the tissue from 960 MHz to 1.1 GHz. Undulating acoustic wave speed (stiffness) distributions corresponded with elastic fibre locations in the tissue; there was a decrease in wave speed of around 40 ms-1 from the adventitia (outer layer) to the intima (innermost).


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