Determination of the total lipid and the long chain omega-3 polyunsaturated fatty acids, EPA and DHA, in deep-sea fish and shark species from the North-East Atlantic

Author(s):  
Peter G. Dunne
Author(s):  
V. Allain

Alepocephalus bairdii is a deep sea fish caught but discarded by semi-industrial fisheries from the north-east Atlantic Ocean. Reproductive features were investigated on 448 females of 29–93 cm standard length (SL) captured over 2 y (December 1995–December 1997) at 800–1280 m off the west coast of the British Isles. The first maturity of females was determined at L50=55 cm SL. A well-defined spawning period could not be determined in this species; a batch spawner characterized by an indeterminate annual fecundity. Absolute batch fecundity was estimated between 458 and 7049 oocytes per female 63–88 cm SL.


2020 ◽  
Vol 27 (27) ◽  
pp. 33658-33658
Author(s):  
Guilherme Teixeira ◽  
Joana Raimundo ◽  
Joana Goulart ◽  
Valentina Costa ◽  
Gui M. Menezes ◽  
...  
Keyword(s):  
Deep Sea ◽  

2020 ◽  
Vol 27 (27) ◽  
pp. 33649-33657
Author(s):  
Guilherme Teixeira ◽  
Joana Raimundo ◽  
Joana Goulart ◽  
Valentina Costa ◽  
Gui M. Menezes ◽  
...  
Keyword(s):  
Deep Sea ◽  

Author(s):  
Anna de Kluijver ◽  
Klaas G.J. Nierop ◽  
Teresa M. Morganti ◽  
Martijn C. Bart ◽  
Beate M. Slaby ◽  
...  

AbstractSponges produce distinct fatty acids (FAs) that (potentially) can be used as chemotaxonomic and ecological biomarkers to study endosymbiont-host interactions and the functional ecology of sponges. Here, we present FA profiles of five common habitat-building deep-sea sponges (class Demospongiae, order Tetractinellida), which are classified as high microbial abundance (HMA) species. Geodia hentscheli, G. parva, G. atlantica, G. barretti, and Stelletta rhaphidiophora were collected from boreal and Arctic sponge grounds in the North-Atlantic Ocean. Bacterial FAs dominated in all five species and particularly isomeric mixtures of mid-chain branched FAs (MBFAs, 8- and 9-Me-C16:0 and 10 and 11-Me-C18:0) were found in high abundance (together ≥ 20% of total FAs) aside more common bacterial markers. In addition, the sponges produced long-chain linear, mid- and a(i)-branched unsaturated FAs (LCFAs) with a chain length of 24‒28 C atoms and had predominantly the typical Δ5,9 unsaturation, although also Δ9,19 and (yet undescribed) Δ11,21 unsaturations were identified. G. parva and S. rhaphidiophora each produced distinct LCFAs, while G. atlantica, G. barretti, and G. hentscheli produced similar LCFAs, but in different ratios. The different bacterial precursors varied in carbon isotopic composition (δ13C), with MBFAs being more enriched compared to other bacterial (linear and a(i)-branched) FAs. We propose biosynthetic pathways for different LCFAs from their bacterial precursors, that are consistent with small isotopic differences found in LCFAs. Indeed, FA profiles of deep-sea sponges can serve as chemotaxonomic markers and support the conception that sponges acquire building blocks from their endosymbiotic bacteria.


2020 ◽  
Vol 265 ◽  
pp. 115060 ◽  
Author(s):  
João M. Pereira ◽  
Yasmina Rodríguez ◽  
Sandra Blasco-Monleon ◽  
Adam Porter ◽  
Ceri Lewis ◽  
...  

2008 ◽  
Vol 59 (3) ◽  
pp. 214 ◽  
Author(s):  
Teresa Moura ◽  
Mónica C. Silva ◽  
Ivone Figueiredo ◽  
Ana Neves ◽  
Pablo Durán Muñoz ◽  
...  

Two genera of elasmobranchii, Centrophorus and Centroscymnus, include species that represent the highest landings of deep-water sharks caught by fishing fleets operating in the north-east Atlantic. There are morphology-based identification problems among and within genera, and landings of processed shark products further prevent an objective assessment of these species. The present study is the first attempt to test the suitability of using a DNA barcode approach to discriminate accurately among the four most important commercial deep-water shark species: Centrophorus squamosus, Centrophorus granulosus, Centroscymnus coelolepis and, recently discovered in Portuguese ports, Centroscymnus owstoni. Sequence analyses of the mitochondrial DNA cytochrome c oxidase subunit I (COI) gene revealed low levels of haplotypic and genetic diversities. Higher levels of inter-specific relative to intra-specific divergences allowed discrimination among species, which form reciprocally monophyletic clades. Inclusion of published COI sequences from other species within the same genera revealed haplotype sharing among species, which calls into question the current taxonomy and accuracy of fisheries data available. Amplification of the COI gene coupled with MboI restriction digests was found to be a fast and inexpensive strategy to resolve within genera identification problems. Molecular barcoding constitutes a critical tool for the assessment and implementation of urgent management policies for this group of species.


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