scholarly journals In vitro risk assessment of Padina pavonica (Linnaeus) (Brown algae)

2021 ◽  
Vol 7 (1) ◽  
pp. 31-38
Author(s):  
Adem Güner
Keyword(s):  
Brown Algae ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Sister Chromatid Exchanges ◽  
Genotoxic Effects ◽  
Anticancer Effects ◽  
Low Concentrations ◽  
Total Antioxidant ◽  
Total Oxidative Status

Padina pavonica (Linnaeus) Thivy 1960 is a brown algae that is antioxidant, antimicrobial, and anticancer effects and is generally used in soup, salad, and other dishes. However, no studies have been reported on safe consumption in humans to date. For this purpose, this study was conducted to determine the cytotoxic and genotoxic effects of P. pavonica on lymphocytes cultured from human blood. The water extract of P. pavonica was added into culture tubes at various concentrations (0.5-1000 μg/mL). Cytotoxic effects were determined by MTT assay. Antioxidant/oxidant status was evaluated by total antioxidant capacity (TAC) and total oxidative status (TOS) assays. Genotoxic effects were investigated by sister chromatid exchanges and micronucleus assays. Our results showed that P. pavonica had no genotoxic effects, even at higher concentrations. 1000 μg/mL concentration of P. pavonica caused an increase (P<0.05) TOS levels while significantly reducing cell viability. However, low concentrations (50 and 100 μg/mL) significantly increased (P<0.05) TAC levels. In conclusion, P. pavonica can be safely consumed with its non-genotoxic and antioxidant properties in a manner dose-dependent.

scholarly journals Apple Pomace Extract as a Sustainable Food Ingredient

Antioxidants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 189 ◽  
Author(s):  
Pedro A. R. Fernandes ◽  
Sónia S. Ferreira ◽  
Rita Bastos ◽  
Isabel Ferreira ◽  
Maria T. Cruz ◽  
...  
Keyword(s):  
Solid Phase ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Apple Pomace ◽  
Water Soluble ◽  
Hot Water ◽  
Array Detector ◽  
Food Ingredient ◽  
Sustainable Solutions

Apple pomace is a by-product of apple processing industries with low value and thus frequent disposal, although with valuable compounds. Acidified hot water extraction has been suggested as a clean, feasible, and easy approach for the recovery of polyphenols. This type of extraction allowed us to obtain 296 g of extract per kg of dry apple pomace, including 3.3 g of polyphenols and 281 g of carbohydrates. Ultrafiltration and solid-phase extraction using C18 cartridges of the hot water extract suggested that, in addition to the apple native polyphenols detected by ultra-high-pressure liquid chromatography coupled to a diode-array detector and mass spectrometry UHPLC-DAD-ESI-MSn, polyphenols could also be present as complexes with carbohydrates. For the water-soluble polyphenols, antioxidant and anti-inflammatory effects were observed by inhibiting chemically generated hydroxyl radicals (OH•) and nitrogen monoxide radicals (NO•) produced in lipopolysaccharide-stimulated macrophages. The water-soluble polyphenols, when incorporated into yogurt formulations, were not affected by fermentation and improved the antioxidant properties of the final product. This in vitro research paves the way for agro-food industries to achieve more diversified and sustainable solutions towards their main by-products.

The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves

2008 ◽  
Vol 116 (3) ◽  
pp. 439-446 ◽  
Author(s):  
Pilaipark Chumark ◽  
Panya Khunawat ◽  
Yupin Sanvarinda ◽  
Srichan Phornchirasilp ◽  
Noppawan Phumala Morales ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Ex Vivo ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Moringa Oleifera Lam

scholarly journals The Effect of Ten Essential Oils on Several Cutaneous Drug-Resistant Microorganisms and Their Cyto/Genotoxic and Antioxidant Properties

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4570 ◽  
Author(s):  
Katarína Kozics ◽  
Mária Bučková ◽  
Andrea Puškárová ◽  
Viktória Kalászová ◽  
Terézia Cabicarová ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antioxidant Properties ◽  
Total Antioxidant Status ◽  
Human Keratinocytes ◽  
Drug Resistant ◽  
Healthy Human ◽  
Antioxidant Agents ◽  
Total Antioxidant ◽  
Antibacterial And Antifungal

In this study, we determined the antimicrobial activity of ten essential oils (EOs)—oregano, thyme, clove, arborvitae, cassia, lemongrass, melaleuca, eucalyptus, lavender, and clary sage—against drug-resistant microorganisms previously isolated from patients with skin infections. The essential oil compositions were determined using gas chromatography coupled to mass spectrometry (GC/MS). The assayed bacteria included Pseudomonas aeruginosa, Proteus vulgaris, Citrobacter koseri, and Klebsiella pneumoniae. Two drug-resistant yeasts (Candida albicans and Candida parapsilosis) were also involved in our survey. Oregano, thyme, cassia, lemongrass and arborvitae showed very strong antibacterial and antifungal activity against all tested strains. These results show that these essential oils may be effective in preventing the growth of the drug-resistant microorganisms responsible for wound infections. In this study, the genotoxic effects of tested essential oils on healthy human keratinocytes HaCaT were evaluated using the comet assay for the first time. These results revealed that none of the essential oils induced significant DNA damage in vitro after 24 h. Moreover, the treatment of HaCaT cells with essential oils increased the total antioxidant status (TAS) level. The obtained results indicate that EOs could be used as a potential source of safe and potent natural antimicrobial and antioxidant agents in the pharmaceutical and food industries.

The Effect of Germination on Antioxidant and Nutritional Parameters of Protein Isolates from Grass Pea (Lathyrus sativus) Seeds

2010 ◽  
Vol 16 (1) ◽  
pp. 73-77 ◽  
Author(s):  
A. Starzyńska-Janiszewska ◽  
B. Stodolak ◽  
B. Mickowska
Keyword(s):  
Antioxidant Properties ◽  
Sulfur Amino Acids ◽  
Grass Pea ◽  
Protein Isolates ◽  
Nutritional Parameters ◽  
Pea Seedlings ◽  
Total Antioxidant ◽  
Pea Seeds ◽  
Potential Food

The aim of this research was to study the antioxidant and nutritional (selected objects) properties of protein isolates obtained from grass pea seedlings as compared with soaked and raw seeds. Two percent extract of isolate from 5-day-old seedlings showed the highest total antioxidant activity (25%) and the ability to chelate Fe 2+ (2.35 mg/g d.m.) as compared with other isolates. Protein isolates from grass pea seeds had on average 89% total protein, 87% in vitro protein bioavailability, about 5574 TIU/g (d.m.) (trypsin inhibitors activity) and did not contain ODAP. Germination of seeds for 5 days considerably improved the in vitro bioavailability of isolates, by 12%, and profile of sulfur amino acids by 42%, in comparison with isolates obtained from the raw seeds. Isolates from 5-day-old grass pea seedlings had the best antioxidant properties and improved nutritional parameters (as compared with raw seeds), which makes them worthy of being considered as a potential food additive.

In vitro antioxidant properties of crude extracts and compounds from brown algae

2013 ◽  
Vol 138 (2-3) ◽  
pp. 1764-1785 ◽  
Author(s):  
Elena M. Balboa ◽  
Enma Conde ◽  
Andres Moure ◽  
Elena Falqué ◽  
Herminia Domínguez
Keyword(s):  
Brown Algae ◽  
Antioxidant Properties ◽  
Crude Extracts

scholarly journals Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of FolkloricLannea coromandelicaUsingIn VitroandIn VivoAssays

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Tekeshwar Kumar ◽  
Vishal Jain
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Intragastric Administration ◽  
Total Antioxidant ◽  
Liver And Kidney ◽  
Mda Content ◽  
Phenolic And Flavonoid

The aim of this study was to determine the impending antioxidant properties of different extracts of crude methanolic extract (CME) of leaves ofLannea coromandelica(L. coromandelica) and its two ethyl acetate (EAF) and aqueous (AqF) subfractions by employing various establishedin vitrosystems and estimation of total phenolic and flavonoid content. The results showed that extract and fractions possessed strong antioxidant activityin vitroand among them, EAF had the strongest antioxidant activity. EAF was confirmed for its highest phenolic content, total flavonoid contents, and total antioxidant capacity. The EAF was found to show remarkable scavenging activity on 2,2-diphenylpicrylhydrazyl (DPPH) (EC5063.9 ± 0.64 µg/mL), superoxide radical (EC508.2 ± 0.12 mg/mL), and Fe2+chelating activity (EC506.2 ± 0.09 mg/mL). Based on ourin vitroresults, EAF was investigated forin vivoantioxidant assay. Intragastric administration of the EAF can significantly increase levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels, and decrease malondialdehyde (MDA) content in the liver and kidney of CCl4-intoxicated rats. These new evidences show thatL. coromandelicabared antioxidant activity.

scholarly journals Anticancer Effects of Novel Tetrahydro-Dimethyl-Xanthene-Diones

2020 ◽  
Vol 20 (7) ◽  
pp. 909-916
Author(s):  
Alagumuthu Manikandan ◽  
Arumugam Sivakumar ◽  
Poonam S. Nigam ◽  
Ayyakannu A. Napoleon
Keyword(s):  
Mtt Assay ◽  
Anticancer Agents ◽  
Antioxidant Properties ◽  
Drug Efficacy ◽  
Anticancer Properties ◽  
Anticancer Effects ◽  
Xanthene Derivatives ◽  
Cancer Types ◽  
In Silico Molecular Docking

Background: The derivatives of xanthene are known to have promising anticancer properties, in comparison to xanthene itself. Objective: The object of our study was to develop few xanthene derivatives (a family of fifteen novel 3,4,6,7- tetrahydro-3,3-dimethyl-9-phenyl-2H-xanthene-1,8(5H, 9H)-diones encoded as 4a-4m), which were effectively prepared through regioselective synthesis approach, and to test their anticancer effects. Methods: A series of cell lines were used in this study, first to assess the cytotoxicity and then the drug efficacy of target compounds, consecutively. Prior to MTT assay, the compounds were analysed for their antioxidant properties, since oxidative stress is an important factor in the development of many cancer types. The anticancer properties of 4a-m have been assessed over in silico (molecular docking and ADMET assessments) and in vitro (MTT assay) methods. Results: Compounds 4h and 4i showed a relative percentage anticancer activity of 86.25±1.25 & 89.74±1.64 against BT474 (ER+HER2+), and 90.56±1.18 & 93.24±1.80 against MCF-7 (ER-HER2), respectively. Conclusion: The animal model and pre-clinical studies for 4h and 4i should be performed in order to develop them as future anticancer agents.

scholarly journals Antioxidant activity of Trachystemon orientalis (L.) G. Don (Borage) grown and eaten as food in Ordu, Turkey

2015 ◽  
Vol 61 (4) ◽  
pp. 40-51 ◽  
Author(s):  
Melek Col Ayvaz
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Total Antioxidant Capacity ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Synthetic Antioxidants ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

SummaryIntroductıon: Instead of synthetic antioxidants, using of natural products with antioxidant activity is demanded.Objectıve: The aim of this study is to evaluate the antioxidant activity and total phenolic and flavonoid contents of theTrachystemon orientalis(borage) obtained from Ordu.Methods: The water and ethanol extracts of the borage plant were investigated by using severalin vitroantioxidant activity tests such as total antioxidant capacity, O2•−,•OH, DPPH and ABTS radicals scavenging activities, hydrogen peroxide scavenging activity, FRAP, Fe2+chelating and reducing power assays in order to make effective comparisons.Results: Water extract of theT. orientalishad the more phenolic (90 mg GA/g extract) and flavonoid (56.88 mg CT/g extract) contents than ethanol extract. Furthermore, both of the extracts showed strong antioxidant and radical scavenging activities determined by differentin vitromodels. The highest total antioxidant capacity as ascorbic acid equivalents of 244.50 mg/g extract was also calculated for water extract. Generally, a relationship between total phenolic content and antioxidant activity was established. It is also an important finding for an edible food source that the phenolic and flavonoid content and antioxidative activities for water extract was higher than the ethanol extract.Conclusıon:T. orientaliscould be used as a food ingredient instead of synthetic antioxidants and all results will contribute to the recent increase in investigations on using natural products in many areas such as food, pharmacy, alternative medicine and natural therapy.

scholarly journals Genotoxic Effects Induced by Fotemustine and Vinorelbine in Human Lymphocytes

2006 ◽  
Vol 61 (11-12) ◽  
pp. 903-910 ◽  
Author(s):  
Serap Çelikler ◽  
Rahmi Bilaloğlu ◽  
Nilüfer Aydemir
Keyword(s):  
Mitotic Index ◽  
Human Lymphocytes ◽  
Vinca Alkaloid ◽  
Test Group ◽  
Sister Chromatid Exchanges ◽  
Specific Response ◽  
Dependent Manner ◽  
Genotoxic Effects ◽  
Dose Dependent

Abstract The aim of this study was to investigate the in vitro genotoxic effects of the anticancer drugs fotemustine and vinorelbine on human lymphocytes and to determine individual and sex-related responses to these drugs. Fotemustine is a DNA-alkylating drug while vinorelbine is a semi-synthetic Vinca alkaloid. The study was carried out with twenty independent healthy donors for each drug. We have tested the ability of these drugs to induce chromosome aberrations (CAs) and sister chromatid exchanges (SCEs) as well as effect on the mitotic index (MI) in cultured human lymphocytes. Fotemustine was shown to induce CAs and SCEs at all concentrations tested (2, 4 and 8 μg/ml) in a dose-dependent manner. Additionally it also decreased the mitotic index in a similar dose-dependent manner. Vinorelbine had no effect on structural CAs, but it significantly increased the numerical CAs at all doses tested (0.5, 1 and 2 μg/ml). Vinorelbine also induced SCE events and increased the MI values. Two-way analyses of variance were used to compare the individual and gender-related susceptibilities to fotemustine and vinorelbine with respect to the CA, SCE and MI values. The results indicated that individuals in fotemustine treatment groups showed different genotoxic responses with respect to CA and SCE induction and additional findings indicated a gender-specific response in this group. Individuals in the vinorelbine test group also exhibited statistically significant numerical CA, SCE and MI responses to vinorelbine. A statistically significant gender-related SCE response to this drug was also evident. This study indicates that these drugs have potentially harmful effects on human health.

scholarly journals New Insights on Hemp Oil Enriched in Cannabidiol: Decarboxylation, Antioxidant Properties and In Vitro Anticancer Effect

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 738
Author(s):  
Anca Roxana Petrovici ◽  
Natalia Simionescu ◽  
Andreea Isabela Sandu ◽  
Vasile Paraschiv ◽  
Mihaela Silion ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Cancer Cells ◽  
Antioxidant Properties ◽  
Flash Chromatography ◽  
Ros Production ◽  
Antioxidant Power ◽  
Anticancer Effects ◽  
Induced Apoptosis ◽  
Hemp Oil

This study aimed to obtain and characterize extracted hemp oil enriched in cannabidiol (CBD) by decarboxylation of cannabidiolic acid (CBDA) and to give new insights into its antioxidant and anticancer effects. Optimization of CBDA decarboxylation in hemp oil was performed, and CBD and CBDA contents and purities were determined by flash chromatography, 1H- and 13C-NMR. The antioxidant properties of CBD-enriched oil were investigated by Fe2+ chelating activity, Fe3+ reducing antioxidant power assay, O2− scavenging activity, HO− scavenging ability and lipid peroxidation inhibitory assay, and its cytotoxicity, apoptosis- and oxidative stress-inducing effects on NHDF, MeWo, HeLa, HepG2 and HOS cells were determined. The CBD concentration in hemp oil was increased by CBDA soft decarboxylation optimized at 90 °C, for 1 h and the resulting oil was capable of reducing iron, scavenging free radicals and inhibiting lipid peroxidation in cell-free oxidative conditions. CBD-enriched oil promoted NHDF proliferation at up to 15 µg CBD/mL, while inducing apoptosis and ROS production and modulating antioxidant enzymes’ gene expression in cancer cells, being selective for osteosarcoma cells, and induced apoptosis by p53- and ROS-independent mechanisms. CBD-enriched hemp oil demonstrated antioxidant properties in oxidative conditions and promoted normal fibroblasts’ proliferation, while inducing apoptosis and ROS production in cancer cells.

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