scholarly journals Detection and Genetic Characterization of Lymphocystis Disease Virus (LCDV) Isolated during Disease Outbreaks in Cultured Gilt-head Sea Bream Sparus aurata in Tunisia

2013 ◽  
Vol 48 (3) ◽  
pp. 101-104 ◽  
Author(s):  
Sond^|^egrave;s Haddad-Boubaker ◽  
Nadia Bouzgarou ◽  
Emna Fakhfakh ◽  
Monia Khayech ◽  
Souhail Ben Mohamed ◽  
...  
Keyword(s):  
Disease Virus ◽  
Genetic Characterization ◽  
Sparus Aurata ◽  
Disease Outbreaks ◽  
Sea Bream ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease

scholarly journals Concurrence of Iridovirus, Polyomavirus, and a Unique Member of a New Group of Fish Papillomaviruses in Lymphocystis Disease-Affected Gilthead Sea Bream

2016 ◽  
Vol 90 (19) ◽  
pp. 8768-8779 ◽  
Author(s):  
Alberto López-Bueno ◽  
Carla Mavian ◽  
Alejandro M. Labella ◽  
Dolores Castro ◽  
Juan J. Borrego ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
Disease Virus ◽  
Fish Species ◽  
Sparus Aurata ◽  
Mediterranean Area ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Lymphocystis Disease Virus ◽  
Content Type ◽  
Lymphocystis Disease

ABSTRACTLymphocystis disease is a geographically widespread disease affecting more than 150 different species of marine and freshwater fish. The disease, provoked by the iridovirus lymphocystis disease virus (LCDV), is characterized by the appearance of papillomalike lesions on the skin of affected animals that usually self-resolve over time. Development of the disease is usually associated with several environmental factors and, more frequently, with stress conditions provoked by the intensive culture conditions present in fish farms. In gilthead sea bream (Sparus aurata), an economically important cultured fish species in the Mediterranean area, a distinct LCDV has been identified but not yet completely characterized. We have used direct sequencing of the virome of lymphocystis lesions from affectedS. auratafish to obtain the complete genome of a new LCDV-Sa species that is the largest vertebrate iridovirus sequenced to date. Importantly, this approach allowed us to assemble the full-length circular genome sequence of two previously unknown viruses belonging to the papillomaviruses and polyomaviruses, termed Sparus aurata papillomavirus 1 (SaPV1) and Sparus aurata polyomavirus 1 (SaPyV1), respectively. Epidemiological surveys showed that lymphocystis disease was frequently associated with the concurrent appearance of one or both of the new viruses. SaPV1 has unique characteristics, such as an intron within the L1 gene, and as the first member of thePapillomaviridaefamily described in fish, provides evidence for a more ancient origin of this family than previously thought.IMPORTANCELymphocystis disease affects marine and freshwater fish species worldwide. It is characterized by the appearance of papillomalike lesions on the skin that contain heavily enlarged cells (lymphocysts). The causative agent is the lymphocystis disease virus (LCDV), a large icosahedral virus of the familyIridoviridae. In the Mediterranean area, the gilthead sea bream (Sparus aurata), an important farmed fish, is frequently affected. Using next-generation sequencing, we have identified withinS. auratalymphocystis lesions the concurrent presence of an additional LCDV species (LCDV-Sa) as well as two novel viruses. These are members of polyomavirus and papillomavirus families, and here we report them to be frequently associated with the presence of lymphocysts in affected fish. Because papillomaviruses have not been described in fish before, these findings support a more ancient origin of this virus family than previously thought and evolutionary implications are discussed.

Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head seabream (Sparus aurata, L.) using an immunoblot technique

2006 ◽  
Vol 113 (1-2) ◽  
pp. 137-141 ◽  
Author(s):  
I. Cano ◽  
M.C. Alonso ◽  
E. Garcia-Rosado ◽  
S. Rodriguez Saint-Jean ◽  
D. Castro ◽  
...  
Keyword(s):  
Disease Virus ◽  
Sparus Aurata ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease ◽  
Immunoblot Technique

scholarly journals Heritability Estimates and Genetic Correlation for Growth Traits and LCDV Susceptibility in Gilthead Sea Bream (Sparus aurata)

Fishes ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 2 ◽  
Author(s):  
Carlos Carballo ◽  
Hyun Suk Shin ◽  
Concepción Berbel ◽  
Maria Jesús Zamorano ◽  
Juan Jose Borrego ◽  
...  
Keyword(s):  
Growth Traits ◽  
Sparus Aurata ◽  
Genetic Correlations ◽  
Genetic Selection ◽  
Severity Index ◽  
The Body ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease

The lymphocystis disease (LCD) is a viral infection with a high economic impact in gilthead sea bream aquaculture. In this study, genetic estimates associated with lymphocystis disease virus (LCDV) disease susceptibility and growth were determined in sea bream juveniles. Two fish batches (named batch 1 and batch 2) were built from mass spawning and reared under industrial conditions until disease outbreak. At the moment of the sampling (n = 500 specimens for each batch), all animals had the typical LCDV lesions in the skin. For phenotyping, animals were weighted and photographed for image analysis (surface covered and lesion intensity). LCDV DNA copies were quantified in the liver by qPCR. Batch 1 had a higher surface covered and lesion intensity than batch 2, and the body caudal region was the lowest affected region in both batches. The average LCDV DNA copies in liver were higher in the batch 1 than batch 2, and they were positively correlated with severity index (SI) categories (r2 = 0.90–0.94). The total number of families evaluated were 150 and 128 for batch 1 and batch 2, respectively, with a high bias in offspring contribution by family and broodstock. Heritabilities for weight and length were 0.18 and 0.14 in batch 1 and 0.06 and 0.05 in batch 2, respectively. Heritability for the number of viral DNA copies was low (<0.08) in both batches. Heritabilities for SI in binary scale were 0.32/0.33 and 0.21/0.24 (underlying liability/Bayesian approach) for batch 1 and batch 2, respectively. Genetic correlations were very high and positive when growth traits (weight and length) or disease traits (LCDV DNA copies and SI) were compared. In contrast, the genetic correlations between growth and disease traits were moderate–high and positive in the batch 1 but negative in batch 2. These results indicate the genetic selection for LCDV susceptibility and growth is feasible in sea bream juveniles, although estimates are highly dependent on the age. The information provided is relevant to designing selective breeding programs in sea bream.

scholarly journals Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata)

2017 ◽  
Vol 48 (1) ◽  
Author(s):  
Estefania J. Valverde ◽  
Juan J. Borrego ◽  
M. Carmen Sarasquete ◽  
Juan B. Ortiz-Delgado ◽  
Dolores Castro
Keyword(s):  
Disease Virus ◽  
Virus Replication ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Lymphocystis Disease Virus ◽  
Target Organs ◽  
Lymphocystis Disease
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