Immune gene expression in gilthead seabream ( Sparus aurata ) after Lymphocystis disease virus (LCDV‐Sa) challenge resulting in asymptomatic infection

2019 ◽  
Vol 128 (1) ◽  
pp. 41-53 ◽  
Author(s):  
R. Leiva‐Rebollo ◽  
A.M. Labella ◽  
J.J. Borrego ◽  
D. Castro
Keyword(s):  
Gene Expression ◽  
Disease Virus ◽  
Sparus Aurata ◽  
Asymptomatic Infection ◽  
Gilthead Seabream ◽  
Immune Gene ◽  
Lymphocystis Disease Virus ◽  
Immune Gene Expression ◽  
Lymphocystis Disease

scholarly journals Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata)

2017 ◽  
Vol 48 (1) ◽  
Author(s):  
Estefania J. Valverde ◽  
Juan J. Borrego ◽  
M. Carmen Sarasquete ◽  
Juan B. Ortiz-Delgado ◽  
Dolores Castro
Keyword(s):  
Disease Virus ◽  
Virus Replication ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Lymphocystis Disease Virus ◽  
Target Organs ◽  
Lymphocystis Disease

scholarly journals Artemia spp., a Susceptible Host and Vector for Lymphocystis Disease Virus

Viruses ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 506 ◽  
Author(s):  
Estefania J. Valverde ◽  
Alejandro M. Labella ◽  
Juan J. Borrego ◽  
Dolores Castro
Keyword(s):  
Gene Expression ◽  
Disease Virus ◽  
Productive Infection ◽  
Most Probable Number ◽  
Gilthead Seabream ◽  
Post Inoculation ◽  
Lymphocystis Disease Virus ◽  
Susceptible Host ◽  
Probable Number ◽  
Lymphocystis Disease

Different developmental stages of Artemia spp. (metanauplii, juveniles and adults) were bath-challenged with two isolates of the Lymphocystis disease virus (LCDV), namely, LCDV SA25 (belonging to the species Lymphocystis disease virus 3) and ATCC VR-342 (an unclassified member of the genus Lymphocystivirus). Viral quantification and gene expression were analyzed by qPCR at different times post-inoculation (pi). In addition, infectious titres were determined at 8 dpi by integrated cell culture (ICC)-RT-PCR, an assay that detects viral mRNA in inoculated cell cultures. In LCDV-challenged Artemia, the viral load increased by 2–3 orders of magnitude (depending on developmental stage and viral isolate) during the first 8–12 dpi, with viral titres up to 2.3 × 102 Most Probable Number of Infectious Units (MPNIU)/mg. Viral transcripts were detected in the infected Artemia, relative expression values showed a similar temporal evolution in the different experimental groups. Moreover, gilthead seabream (Sparus aurata) fingerlings were challenged by feeding on LCDV-infected metanauplii. Although no Lymphocystis symptoms were observed in the fish, the number of viral DNA copies was significantly higher at the end of the experimental trial and major capsid protein (mcp) gene expression was consistently detected. The results obtained support that LCDV infects Artemia spp., establishing an asymptomatic productive infection at least under the experimental conditions tested, and that the infected metanauplii are a vector for LCDV transmission to gilthead seabream.

scholarly journals Antiviral activity of Mx proteins from gilthead seabream (Sparus aurata) against lymphocystis disease virus (LCDV)

2013 ◽  
Vol 34 (6) ◽  
pp. 1651
Author(s):  
M.A. Fernández-Trujillo ◽  
E. García-Rosado ◽  
M.C. Alonso ◽  
J.J. Borrego ◽  
M.C. Álvarez ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Disease Virus ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease

Transmission of lymphocystis disease virus to cultured gilthead seabream, Sparus aurata L., larvae

2012 ◽  
Vol 36 (6) ◽  
pp. 569-576 ◽  
Author(s):  
I Cano ◽  
E J Valverde ◽  
E Garcia-Rosado ◽  
M C Alonso ◽  
B Lopez-Jimena ◽  
...  
Keyword(s):  
Disease Virus ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease

Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head seabream (Sparus aurata, L.) using an immunoblot technique

2006 ◽  
Vol 113 (1-2) ◽  
pp. 137-141 ◽  
Author(s):  
I. Cano ◽  
M.C. Alonso ◽  
E. Garcia-Rosado ◽  
S. Rodriguez Saint-Jean ◽  
D. Castro ◽  
...  
Keyword(s):  
Disease Virus ◽  
Sparus Aurata ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease ◽  
Immunoblot Technique
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