scholarly journals Indirect Immunoperoxidase Technique for the Diagnosis of Cold-water Disease in Cultured Ayu, Plecoglossus altivelis.

1998 ◽  
Vol 33 (3) ◽  
pp. 149-150 ◽  
Author(s):  
Hideaki Aikawa
Keyword(s):  
Cold Water ◽  
Immunoperoxidase Technique ◽  
Plecoglossus Altivelis ◽  
Indirect Immunoperoxidase

scholarly journals Varicella-zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique

1976 ◽  
Vol 4 (5) ◽  
pp. 437-442
Author(s):  
G Gerna ◽  
R W Chambers
Keyword(s):  
Neutralization Test ◽  
Plaque Assay ◽  
Neutralizing Antibody ◽  
Immunoperoxidase Technique ◽  
Serum Samples ◽  
Plaque Reduction Neutralization Test ◽  
Varicella Zoster ◽  
Convalescent Phase ◽  
Antibody Titers ◽  
Indirect Immunoperoxidase

A new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed using the indirect immunoperoxidase technique. As compared with the classical plaque assay using a solid overlay, the test gives earlier results since plaque counting can be performed on day 3 after the inoculation of cell cultures. In six patients with zoster infection, neutralizing antibody titers ranged from 1:20 to 1:40 before the onset of infection and reached high levels (1:320 to 1:5,120) during the convalescent phase of the disease. Complement-fixing (CF) titers were all negative (less than 1:8) in prezoster serum samples from the same patients and ranged from 1:128 to 1:2,048 in the convalescent-phase sera. In the two cases in which late serum samples were available, neutralizing antibody titers matched the preillness levels, whereas CF titers dropped to undetectable levels. Neither neutralizing nor CF antibody was detected in two sera from individuals with no history of varicella-zoster infection. No differences in virus titers or neutralizing antibody titers were observed between the immunoperoxidase and the classical plaque assays. The appropriate characterization of reagent specificity is required before routine application of the test.

Use of a Pool of Monoclonal Antibodies in Diagnosing Cells from Serous Cavities

1993 ◽  
Vol 79 (3) ◽  
pp. 211-213
Author(s):  
Antonio Cappellari ◽  
Mariangela Bagarella ◽  
Sylvie Ménard ◽  
Marcella Calabrese ◽  
Maria Ines Colnaghi
Keyword(s):  
Monoclonal Antibodies ◽  
Traditional Method ◽  
Immunoperoxidase Technique ◽  
Malignant Cells ◽  
Lung Cancers ◽  
Epithelial Origin ◽  
Neoplastic Lesions ◽  
Immunocytochemical Method ◽  
Indirect Immunoperoxidase ◽  
Diagnostic Investigations

Aims and Background The usefulness of monoclonal antibodies that recognize markers of neoplastic lesions in complementing conventional cytology was evaluated by the avidin-biotin-peroxidase complex, indirect immunoperoxidase technique. Methods In order to enhance the sensitivity of the traditional method, a pool of seven combined monoclonal antibodies (Pool C7), which reacts specifically with cells of epithelial origin and is able to distinguish between mesothelial and malignant cells, was tested on cytologic smears of 262 serous effusions. The effusions were benign or neoplastic, mainly from breast, ovary and lung cancers. Results Immunocytochemical method showed an 100 % specificity and 100 % of predictivity whereas the sensitivity was 98 %, 96 % and 95 % for breast ovarian and lung carcinomas, respectively. Conclusions The results demonstrated that the pool when used together with conventional methods, is useful in analysis of serous effusions in diagnostic investigations.

scholarly journals On the quantitation of SV40 virus by plaque assay and immunoperoxidase technique.

1978 ◽  
Vol 26 (9) ◽  
pp. 755-758 ◽  
Author(s):  
R M D'Alisa ◽  
E L Gershey
Keyword(s):  
Plaque Assay ◽  
T Antigen ◽  
Plaque Formation ◽  
Virus Infectivity ◽  
Immunoperoxidase Technique ◽  
Sv40 Virus ◽  
Antigen Assay ◽  
Indirect Immunoperoxidase

Procedures are described for the quantitation of SV40 virus infectivity by plaque formation within 7 days and T antigen assay by the sensitive and economical indirect immunoperoxidase technique.

scholarly journals Validation of the biotinyl ligand-avidin-gold technique.

1992 ◽  
Vol 40 (5) ◽  
pp. 711-721 ◽  
Author(s):  
R E Morris ◽  
G M Ciraolo ◽  
C B Saelinger
Keyword(s):  
Intracellular Trafficking ◽  
Receptor Interaction ◽  
Immunoperoxidase Technique ◽  
Coated Pits ◽  
Pseudomonas Exotoxin ◽  
Gold Colloids ◽  
Lysosomal Compartment ◽  
Pseudomonas Exotoxin A ◽  
Indirect Immunoperoxidase ◽  
Dense Marker

We demonstrate here that the intracellular routing of biotinylated ligands was not affected by the attachment of streptavidin gold colloids so long as the electron-dense marker was added after the biotinyl ligand-receptor interaction had occurred. The binding, internalization, and intracellular routing of three different biotinyl ligands were followed in mouse LM fibroblasts. The biotinyl (B) ligands included B-choleragenoid (B-CTd), B-wheat germ agglutinin (B-WGA), and B-Pseudomonas exotoxin A (B-PE). All three ligands showed distinct intracellular trafficking patterns. B-WGA and B-PE entered via clathrin-coated pits, whereas B-CTd did not. After entry, B-CTd was routed to the lysosomal compartment without involvement of the Golgi. Although B-PE and B-WGA were also routed to the lysosomal compartment, a significant portion of these two ligands was observed in association with the Golgi. B-WGA, however, remained in the endosomal and Golgi compartments longer than did B-PE. We also monitored the internalization and routing of native PE by an indirect immunoperoxidase technique done in conjunction with saponin solubilization. The results corroborated the observations with the biotinyl-PE-streptavidin-gold method. In contrast, biotinyl-PE added to streptavidin-gold before addition to LM cells was poorly internalized and routed aberrantly. From these observations we conclude that the biotinyl ligand-avidin-gold technique is a valid method for following the binding, internalization, and intracellular routing of ligands.

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