Sites of prealbumin production in the human fetus using the indirect immunoperoxidase technique

1985 ◽  
Vol 406 (4) ◽  
pp. 463-473 ◽  
Author(s):  
Hugh D. A. Gray ◽  
Elizabeth S. Gray ◽  
Charles H. W. Horne
Keyword(s):  
Human Fetus ◽  
Immunoperoxidase Technique ◽  
Indirect Immunoperoxidase

scholarly journals Varicella-zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique

1976 ◽  
Vol 4 (5) ◽  
pp. 437-442
Author(s):  
G Gerna ◽  
R W Chambers
Keyword(s):  
Neutralization Test ◽  
Plaque Assay ◽  
Neutralizing Antibody ◽  
Immunoperoxidase Technique ◽  
Serum Samples ◽  
Plaque Reduction Neutralization Test ◽  
Varicella Zoster ◽  
Convalescent Phase ◽  
Antibody Titers ◽  
Indirect Immunoperoxidase

A new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed using the indirect immunoperoxidase technique. As compared with the classical plaque assay using a solid overlay, the test gives earlier results since plaque counting can be performed on day 3 after the inoculation of cell cultures. In six patients with zoster infection, neutralizing antibody titers ranged from 1:20 to 1:40 before the onset of infection and reached high levels (1:320 to 1:5,120) during the convalescent phase of the disease. Complement-fixing (CF) titers were all negative (less than 1:8) in prezoster serum samples from the same patients and ranged from 1:128 to 1:2,048 in the convalescent-phase sera. In the two cases in which late serum samples were available, neutralizing antibody titers matched the preillness levels, whereas CF titers dropped to undetectable levels. Neither neutralizing nor CF antibody was detected in two sera from individuals with no history of varicella-zoster infection. No differences in virus titers or neutralizing antibody titers were observed between the immunoperoxidase and the classical plaque assays. The appropriate characterization of reagent specificity is required before routine application of the test.

Use of a Pool of Monoclonal Antibodies in Diagnosing Cells from Serous Cavities

1993 ◽  
Vol 79 (3) ◽  
pp. 211-213
Author(s):  
Antonio Cappellari ◽  
Mariangela Bagarella ◽  
Sylvie Ménard ◽  
Marcella Calabrese ◽  
Maria Ines Colnaghi
Keyword(s):  
Monoclonal Antibodies ◽  
Traditional Method ◽  
Immunoperoxidase Technique ◽  
Malignant Cells ◽  
Lung Cancers ◽  
Epithelial Origin ◽  
Neoplastic Lesions ◽  
Immunocytochemical Method ◽  
Indirect Immunoperoxidase ◽  
Diagnostic Investigations

Aims and Background The usefulness of monoclonal antibodies that recognize markers of neoplastic lesions in complementing conventional cytology was evaluated by the avidin-biotin-peroxidase complex, indirect immunoperoxidase technique. Methods In order to enhance the sensitivity of the traditional method, a pool of seven combined monoclonal antibodies (Pool C7), which reacts specifically with cells of epithelial origin and is able to distinguish between mesothelial and malignant cells, was tested on cytologic smears of 262 serous effusions. The effusions were benign or neoplastic, mainly from breast, ovary and lung cancers. Results Immunocytochemical method showed an 100 % specificity and 100 % of predictivity whereas the sensitivity was 98 %, 96 % and 95 % for breast ovarian and lung carcinomas, respectively. Conclusions The results demonstrated that the pool when used together with conventional methods, is useful in analysis of serous effusions in diagnostic investigations.

scholarly journals On the quantitation of SV40 virus by plaque assay and immunoperoxidase technique.

1978 ◽  
Vol 26 (9) ◽  
pp. 755-758 ◽  
Author(s):  
R M D'Alisa ◽  
E L Gershey
Keyword(s):  
Plaque Assay ◽  
T Antigen ◽  
Plaque Formation ◽  
Virus Infectivity ◽  
Immunoperoxidase Technique ◽  
Sv40 Virus ◽  
Antigen Assay ◽  
Indirect Immunoperoxidase

Procedures are described for the quantitation of SV40 virus infectivity by plaque formation within 7 days and T antigen assay by the sensitive and economical indirect immunoperoxidase technique.

scholarly journals Validation of the biotinyl ligand-avidin-gold technique.

1992 ◽  
Vol 40 (5) ◽  
pp. 711-721 ◽  
Author(s):  
R E Morris ◽  
G M Ciraolo ◽  
C B Saelinger
Keyword(s):  
Intracellular Trafficking ◽  
Receptor Interaction ◽  
Immunoperoxidase Technique ◽  
Coated Pits ◽  
Pseudomonas Exotoxin ◽  
Gold Colloids ◽  
Lysosomal Compartment ◽  
Pseudomonas Exotoxin A ◽  
Indirect Immunoperoxidase ◽  
Dense Marker

We demonstrate here that the intracellular routing of biotinylated ligands was not affected by the attachment of streptavidin gold colloids so long as the electron-dense marker was added after the biotinyl ligand-receptor interaction had occurred. The binding, internalization, and intracellular routing of three different biotinyl ligands were followed in mouse LM fibroblasts. The biotinyl (B) ligands included B-choleragenoid (B-CTd), B-wheat germ agglutinin (B-WGA), and B-Pseudomonas exotoxin A (B-PE). All three ligands showed distinct intracellular trafficking patterns. B-WGA and B-PE entered via clathrin-coated pits, whereas B-CTd did not. After entry, B-CTd was routed to the lysosomal compartment without involvement of the Golgi. Although B-PE and B-WGA were also routed to the lysosomal compartment, a significant portion of these two ligands was observed in association with the Golgi. B-WGA, however, remained in the endosomal and Golgi compartments longer than did B-PE. We also monitored the internalization and routing of native PE by an indirect immunoperoxidase technique done in conjunction with saponin solubilization. The results corroborated the observations with the biotinyl-PE-streptavidin-gold method. In contrast, biotinyl-PE added to streptavidin-gold before addition to LM cells was poorly internalized and routed aberrantly. From these observations we conclude that the biotinyl ligand-avidin-gold technique is a valid method for following the binding, internalization, and intracellular routing of ligands.

scholarly journals Involvement of contractile proteins in the changes in consistency of oocyte nucleoplasm of the newt Pleurodeles waltlii.

1981 ◽  
Vol 88 (2) ◽  
pp. 410-421 ◽  
Author(s):  
P Gounon ◽  
E Karsenti
Keyword(s):  
Electron Microscope ◽  
Oocyte Nucleus ◽  
Immunoperoxidase Technique ◽  
Actin Microfilaments ◽  
Nuclear Actin ◽  
Filamentous Form ◽  
Associated Proteins ◽  
Pleurodeles Waltlii ◽  
Indirect Immunoperoxidase ◽  
Actin Cables

In the present work, we show that actin is present in considerable quantities in the oocyte nucleus of the newt Pleurodeles waltlii. The nuclear sap, extracted in saline buffer containing Ca++, is fluid. DNAase I inhibition assays have shown that 90% of actin is under a globular state in such conditions. Chelation of Ca++ by EGTA leads to the formation of a nuclear gel composed of individual microfilaments. This nuclear gel contains approximately 50% of total nuclear actin in a filamentous form. Phalloidin, a drug known to stabilize F-actin, induces the formation of a network of actin cables in the nuclei. This network contains nearly 100% of total nuclear actin in the filamentous form. The observation of the cables in the electron microscope shows that they are made of tightly associated microfilaments to which RNP-like particles are bound. The actin antibodies stain the cables and the particles by the indirect immunoperoxidase technique; myosin antibodies mainly stain the particles. The formation of the phalloïdin-induced network seems to require the presence of Ca++, Mg++, and ATP. We propose a scheme for the regulation of the supramolecular forms of actin in oocyte nuclei in which a delicate equilibrium seems to exist between globular actin, microfilaments, and actin cables. This equilibrium would be controlled by the concentration of Ca++, ATP, and various actin-associated proteins.

scholarly journals Identification of contractile proteins in basal bodies of ciliated tracheal epithelial cells.

1980 ◽  
Vol 28 (11) ◽  
pp. 1189-1197 ◽  
Author(s):  
R E Gordon ◽  
B P Lane ◽  
F Miller
Keyword(s):  
Basal Body ◽  
Contractile Function ◽  
Ultrastructural Localization ◽  
Immunoperoxidase Technique ◽  
Basal Bodies ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial ◽  
Indirect Immunoperoxidase ◽  
Basal Foot ◽  
Basal Density

To determine the molecular composition of the components of basal bodies and the interbasal body apparatus of ciliated cells in rat tracheal epithelium, we used rabbit anti-actin, anti-alpha-actinin, anti-tropomyosin, and anti-myosin as primary antisera applied to the tissue in an indirect immunoperoxidase technique. The antisera was proven to be monospecific by elution of antibody after affinity chromatography. Sheep anti-rabbit immunoglobulin Fab fragments coupled to peroxidase were used for ultrastructural localization of the bound rabbit antibody. Antibodies against alpha-actinin were demonstrated around peripheral microtubules of cilia and linking these microtubules to central doublet and plasma membrane. Alpha-actinin was also shown in the basal foot processes. Anti-actin antibodies were associated with microtubules of the cilium and basal bodies, except in the region of the ciliary necklace. The antibodies directed against actin also had affinity for rootlets, basal foot processes, and communications between basal bodies and foot processes. Both anti-myosin and anti-tropomyosin antibodies were localized to part of the region of the constriction of the cilium, to the central basal density and the outer surfaces of basal body microtubules, and to the basal foot processes together with their communications to the basal body. The data suggest active contractile function of basal bodies.

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