scholarly journals Location of Vibrio anguillarum in tissues of infected rainbow trout (Salmo gairdneri) using the fluorescent antibody technique.

1985 ◽  
Vol 20 (2/3) ◽  
pp. 229-235 ◽  
Author(s):  
Jim S. NELSON ◽  
John S. ROHOVEC ◽  
John L. FRYER
Keyword(s):  
Rainbow Trout ◽  
Fluorescent Antibody ◽  
Vibrio Anguillarum ◽  
Salmo Gairdneri ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique

Indirect Fluorescent Antibody Technique for Detection of RM Bacterium of Rainbow Trout (Salmo gairdneri)

1974 ◽  
Vol 31 (12) ◽  
pp. 1957-1959 ◽  
Author(s):  
G. R. Johnson ◽  
G. Wobeser ◽  
B. T. Rouse
Keyword(s):  
Rainbow Trout ◽  
Rapid Detection ◽  
Fluorescent Antibody ◽  
Salmo Gairdneri ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique

An indirect fluorescent antibody technique was evaluated as a method for the rapid detection of RM bacterium of rainbow trout (Salmo gairdneri). Antibody produced by inoculating rabbits with washed RM bacterium antigen was combined with commercially prepared fluorescein-labelled antiglobulin. The technique demonstrated strong specificity for the RM bacterium in washed and broth cultures, and in kidney smears from experimentally infected rainbow trout.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection

scholarly journals LOCALIZATION OF UROMUCOID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHNIQUE

1965 ◽  
Vol 13 (3) ◽  
pp. 155-160 ◽  
Author(s):  
H. J. KEUTEL
Keyword(s):  
Kidney Stones ◽  
Kidney Stone ◽  
Fluorescent Antibody ◽  
Human Kidney ◽  
Fluorescent Antibody Technique ◽  
Renal Tubules ◽  
Antibody Technique ◽  
The Matrix ◽  
Normal Human ◽  
The Common

Fluorescent labeled antibodies were used for the demonstration of uromucoid. This urine specific mucoprotein is demonstrably present only in the epithelial cells of the proximal segments of the normal human renal tubules and in the matrix of human kidney stones of all the common crystalline compositions.

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