Indirect Fluorescent Antibody Technique for Detection of RM Bacterium of Rainbow Trout (Salmo gairdneri)

1974 ◽  
Vol 31 (12) ◽  
pp. 1957-1959 ◽  
Author(s):  
G. R. Johnson ◽  
G. Wobeser ◽  
B. T. Rouse
Keyword(s):  
Rainbow Trout ◽  
Rapid Detection ◽  
Fluorescent Antibody ◽  
Salmo Gairdneri ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique

An indirect fluorescent antibody technique was evaluated as a method for the rapid detection of RM bacterium of rainbow trout (Salmo gairdneri). Antibody produced by inoculating rabbits with washed RM bacterium antigen was combined with commercially prepared fluorescein-labelled antiglobulin. The technique demonstrated strong specificity for the RM bacterium in washed and broth cultures, and in kidney smears from experimentally infected rainbow trout.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection

scholarly journals Toxoplasma antibodies in immigrants from Hong Kong

1974 ◽  
Vol 72 (1) ◽  
pp. 19-22 ◽  
Author(s):  
S. K. K. Seah
Keyword(s):  
Hong Kong ◽  
Toxoplasma Gondii ◽  
Indigenous Population ◽  
Fluorescent Antibody ◽  
Age Groups ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Chinese Adults ◽  
Antibody Technique ◽  
Total Incidence

SUMMARYThe sera of 200 Chinese adults recently immigrated to Canada from Hong Kong were examined for antibodies to Toxoplasma gondii by the indirect fluorescent antibody technique. It was found that the total incidence was 18·0%. This is significantly lower than that of the indigenous population of the same age groups in this area.

scholarly journals Diagnosis of Avian Infectious Bronchitis by Indirect Fluorescent Antibody Technique Using Monoclonal Antibody

1993 ◽  
Vol 46 (2) ◽  
pp. 117-119 ◽  
Author(s):  
Kazuki ISHIBASHI ◽  
Hitomi SHIRAKAWA ◽  
Yoshifumi TOMISHITA ◽  
Hiroyuki MATUO ◽  
Akira WATANABE ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Infectious Bronchitis

scholarly journals Fluorescent antibody test for the serological diagnosis of gonorrhea

1976 ◽  
Vol 3 (4) ◽  
pp. 438-442
Author(s):  
H A Gaafar ◽  
D C D'Arcangelis
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Immunoglobulin G ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Serological Diagnosis ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Normal Individuals ◽  
Male Patients

An indirect fluorescent antibody technique has been developed for the serological diagnosis of gonorrhea. The selected strain(s) of Neisseria gonorrhoeae possesses a heat-labile surface antigen (L-antigen). Sera are diluted 1:10, and an aliquot is hear inactivated at 59 C for 30 min. The treated and untreated aliquots are then examined for human immunoglobulin G anti-L-antigen. In a prelimiary study of 495 sera, 95% of those from women with a bacteriologically confirmed diagnosis of gorrhea and 87% of those from male patients were reactive in this test, whereas only 1.4% fo the sera from presumably normal individuals were reactive.

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