scholarly journals A Microfluidic Device Combining Dielectrophoresis and Field Flow Fractionation for Particle and Cell Discrimination

1998 ◽  
Author(s):  
P. Gascoyne ◽  
X. Wang ◽  
H. Ackler ◽  
S. Swierkowski ◽  
P. Krulevitch ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Field Flow Fractionation ◽  
Flow Fractionation ◽  
Cell Discrimination

Model-based analysis of a dielectrophoretic microfluidic device for field-flow fractionation

2016 ◽  
Vol 39 (15) ◽  
pp. 3028-3036 ◽  
Author(s):  
Bobby Mathew ◽  
Anas Alazzam ◽  
Mohammad Abutayeh ◽  
Ion Stiharu
Keyword(s):  
Microfluidic Device ◽  
Field Flow Fractionation ◽  
Model Based ◽  
Flow Fractionation ◽  
Model Based Analysis

Path of microparticles in a microfluidic device employing dielectrophoresis for hyperlayer field-flow fractionation

2016 ◽  
Vol 22 (7) ◽  
pp. 1721-1732 ◽  
Author(s):  
Bobby Mathew ◽  
Anas Alazzam ◽  
Saud Khashan ◽  
Bashar El-Khasawneh
Keyword(s):  
Microfluidic Device ◽  
Field Flow Fractionation ◽  
Flow Fractionation

Modeling the trajectory of microparticles subjected to dielectrophoresis in a microfluidic device for field flow fractionation

2015 ◽  
Vol 138 ◽  
pp. 266-280 ◽  
Author(s):  
Bobby Mathew ◽  
Anas Alazzam ◽  
Mohammad Abutayeh ◽  
Amjad Gawanmeh ◽  
Saud Khashan
Keyword(s):  
Microfluidic Device ◽  
Field Flow Fractionation ◽  
Flow Fractionation

Micro-Thermal Field-Flow Fractionation

2002 ◽  
Vol 67 (11) ◽  
pp. 1596-1608 ◽  
Author(s):  
Josef Janča
Keyword(s):  
High Performance ◽  
Thermal Field ◽  
Temperature Drop ◽  
Point Of View ◽  
Constant Field ◽  
Field Flow Fractionation ◽  
Operational Mode ◽  
Experimental Conditions ◽  
Standard Size ◽  
Flow Fractionation

The effect of miniaturization of the separation channel on the performance of thermal field-flow fractionation (TFFF) is substantiated theoretically. The experiments carried out under carefully chosen experimental conditions proved the high performance of the separation of polymers within an extended range of molar masses from relatively low up to ultrahigh-molar-mass (UHMM) samples. The new micro-TFFF allows to achieve high resolution when applying constant field force operation, it makes easy the programming of the temperature drop which is an advantageous operational mode from the point of view of the time of analysis, and it extends considerably the range of perfectly controlled temperature of the cold wall due to a substantial decrease in the heat energy flux compared with standard size channels.

scholarly journals Limitations of Nanoparticles Size Characterization by Asymmetric Flow Field‑Fractionation Coupled with Online Dynamic Light Scattering

2021 ◽  
Author(s):  
Francesco Giorgi ◽  
Judith M. Curran ◽  
Douglas Gilliland ◽  
Rita La Spina ◽  
Maurice Whelan ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Flow Field ◽  
Field Flow Fractionation ◽  
Asymmetric Flow ◽  
Uv Detector ◽  
Elution Time ◽  
On Line ◽  
Real Size ◽  
Flow Fractionation

AbstractThe development of reliable protocols suitable for the characterisation of the physical properties of nanoparticles in suspension is becoming crucial to assess the potential biological as well as toxicological impact of nanoparticles. Amongst sizing techniques, asymmetric flow field flow fractionation (AF4) coupled to online size detectors represents one of the most robust and flexible options to quantify the particle size distribution in suspension. However, size measurement uncertainties have been reported for on-line dynamic light scattering (DLS) detectors when coupled to AF4 systems. In this work we investigated the influence of the initial concentration of nanoparticles in suspension on the sizing capability of the asymmetric flow field-flow fractionation technique coupled with an on-line dynamic light scattering detector and a UV–Visible spectrophotometer (UV) detector. Experiments were performed with suspensions of gold nanoparticles with a nominal diameter of 40 nm and 60 nm at a range of particle concentrations. The results obtained demonstrate that at low concentration of nanoparticles, the AF4-DLS combined technique fails to evaluate the real size of nanoparticles in suspension, detecting an apparent and progressive size increase as a function of the elution time and of the concentration of nanoparticles in suspension.

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