Changes in phenolic composition and bioactivity of raw and pickled cowpea (Vigna unguiculata L. Walp.) green pod after in vitro simulated gastrointestinal digestion

2021 ◽  
Vol 27 (5) ◽  
pp. 769-778
Author(s):  
Shirong Huang ◽  
Huan Liu ◽  
Sinian Yan ◽  
Dongfang Chen ◽  
Xin Mei
Keyword(s):  
Vigna Unguiculata ◽  
Phenolic Composition ◽  
Gastrointestinal Digestion ◽  
Simulated Gastrointestinal Digestion

Anti-inflammatory and antioxidant effects of peptides released from germinated amaranth during in vitro simulated gastrointestinal digestion

2021 ◽  
Vol 343 ◽  
pp. 128394
Author(s):  
Eslim Sugey Sandoval-Sicairos ◽  
Ada Keila Milán-Noris ◽  
Diego Armando Luna-Vital ◽  
Jorge Milán-Carrillo ◽  
Alvaro Montoya-Rodríguez
Keyword(s):  
Gastrointestinal Digestion ◽  
Simulated Gastrointestinal Digestion ◽  
Anti Inflammatory ◽  
Antioxidant Effects

scholarly journals Novel ACE Inhibitory Peptides Derived from Simulated Gastrointestinal Digestion in Vitro of Sesame (Sesamum indicum L.) Protein and Molecular Docking Study

2020 ◽  
Vol 21 (3) ◽  
pp. 1059 ◽  
Author(s):  
Ruidan Wang ◽  
Xin Lu ◽  
Qiang Sun ◽  
Jinhong Gao ◽  
Lin Ma ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Docking ◽  
Ace Inhibition ◽  
Gastrointestinal Digestion ◽  
Ace Inhibitory Activity ◽  
Inhibitory Peptides ◽  
Simulated Gastrointestinal Digestion ◽  
Ace Inhibitory Peptides ◽  
Ace Inhibitory

The aim of this study was to isolate and identify angiotensin I-converting enzyme (ACE) inhibitory peptides from sesame protein through simulated gastrointestinal digestion in vitro, and to explore the underlying mechanisms by molecular docking. The sesame protein was enzymatically hydrolyzed by pepsin, trypsin, and α-chymotrypsin. The degree of hydrolysis (DH) and peptide yield increased with the increase of digest time. Moreover, ACE inhibitory activity was enhanced after digestion. The sesame protein digestive solution (SPDS) was purified by ultrafiltration through different molecular weight cut-off (MWCO) membranes and SPDS-VII (< 3 kDa) had the strongest ACE inhibition. SPDS-VII was further purified by NGC Quest™ 10 Plus Chromatography System and finally 11 peptides were identified by Nano UHPLC-ESI-MS/MS (nano ultra-high performance liquid chromatography-electrospray ionization mass spectrometry/mass spectrometry) from peak 4. The peptide GHIITVAR from 11S globulin displayed the strongest ACE inhibitory activity (IC50 = 3.60 ± 0.10 μM). Furthermore, the docking analysis revealed that the ACE inhibition of GHIITVAR was mainly attributed to forming very strong hydrogen bonds with the active sites of ACE. These results identify sesame protein as a rich source of ACE inhibitory peptides and further indicate that GHIITVAR has the potential for development of new functional foods.

The effect of in vitro simulated gastrointestinal digestion on phenolic bioaccessibility and bioactivities of Prinsepia utilis Royle fruits

LWT ◽  
2021 ◽  
Vol 138 ◽  
pp. 110782
Author(s):  
Xiaojing Liu ◽  
Jiyuan Shi ◽  
Junjie Yi ◽  
Xuan Zhang ◽  
Qian Ma ◽  
...  
Keyword(s):  
Gastrointestinal Digestion ◽  
Simulated Gastrointestinal Digestion
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