scholarly journals IMPACT OF BOTANICAL INSECTICIDES DERIVED FROM Pangium edule Reinw AND Annona muricata L. SEED EXTRACTS ON THE “GAY GANTUNG” DIAMONDBACK MOTH, Plutella xylostella L.

2017 ◽  
Vol 2 (2) ◽  
pp. 27-35
Author(s):  
Ernest Hanny Sakul
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Probit Analysis ◽  
Seed Extract ◽  
Hexane Fraction ◽  
Botanical Insecticides ◽  
Annona Muricata ◽  
Active Components ◽  
Botanical Pesticides ◽  
Randomized Design

Insecticidal properties of fractioned extracts from Pangium edule Reinw seed and Annona muricata seed against Plutella xylostella larvae were investigated in the laboratory. The study was initiated to investigate the possibility of using botanical pesticides to control P.xylostella, a serious cosmopolitan pest of crucifer plants. The study aims to determine the most effective concentration and the most active extract;  to evaluate the different extract concentrations on the treated larvae; and to characterize the phytochemical contents of the most effective extracts fraction. The study was an experiment initiated by test of phytochemical screening test in order to discover the presence of secondary metabolites in the extracts. It was followed by the test of mortality of the diamondback moth larvae. Furthermore, the extracts hexane fraction and etanol fraction, were conducted with completely randomized design; The LC50 values were determined following probit analysis, the data were treated in the software programme IBM Statistic SPSS 20. Results showed that n-hexane fraction is the most effective againts larvae (LC50-48h = 12,71 mg/L) from P.edule seed extract, (LC50-48h = 50,81 mg/L) from A.muricata seed extract. Larva mortality was highest using 1000 ppm n-hexane fraction (96,6%) derived from P.edule seed extract, (93,3%) derived from A.muricata seed extract. The ethanol fraction tested positive for alkaloid, saponins, flavonoids, terpenoids, phenol and tannins. N-hexane fraction of P.edule seed extract, and A.muricata seed extract are an effective botanical insecticides exhibiting larvicidal and antifeedant properties against P.xylostella thus it can be alternative to synthethic insecticides. Results indicate that these botanical insecticides have good possibilities for control of P.xylostella. Further work is necessary to evaluate and characterize the active components of the extract fractions and its efficacy in the fieldInsecticidal properties of fractioned extracts from Pangium edule Reinw seed and Annona muricata seed against Plutella xylostella larvae were investigated in the laboratory. The study was initiated to investigate the possibility of using botanical pesticides to control P.xylostella, a serious cosmopolitan pest of crucifer plants. The study aims to determine the most effective concentration and the most active extract;  to evaluate the different extract concentrations on the treated larvae; and to characterize the phytochemical contents of the most effective extracts fraction. The study was an experiment initiated by test of phytochemical screening test in order to discover the presence of secondary metabolites in the extracts. It was followed by the test of mortality of the diamondback moth larvae. Furthermore, the extracts hexane fraction and etanol fraction, were conducted with completely randomized design; The LC50 values were determined following probit analysis, the data were treated in the software programme IBM Statistic SPSS 20. Results showed that n-hexane fraction is the most effective againts larvae (LC50-48h = 12,71 mg/L) from P.edule seed extract, (LC50-48h = 50,81 mg/L) from A.muricata seed extract. Larva mortality was highest using 1000 ppm n-hexane fraction (96,6%) derived from P.edule seed extract, (93,3%) derived from A.muricata seed extract. The ethanol fraction tested positive for alkaloid, saponins, flavonoids, terpenoids, phenol and tannins. N-hexane fraction of P.edule seed extract, and A.muricata seed extract are an effective botanical insecticides exhibiting larvicidal and antifeedant properties against P.xylostella thus it can be alternative to synthethic insecticides. Results indicate that these botanical insecticides have good possibilities for control of P.xylostella. Further work is necessary to evaluate and characterize the active components of the extract fractions and its efficacy in the field

scholarly journals Control Pest of Leaf Caterpillars (Plutella xylostella) in Delima Rose Apples Using Soursop Leaf Extract (Annona muricata)

2018 ◽  
Vol 7 (1) ◽  
pp. 1-8 ◽  
Author(s):  
A. V. Amalia ◽  
M. H. Yusa
Keyword(s):  
Plutella Xylostella ◽  
Leaf Extract ◽  
Probit Analysis ◽  
Effective Concentration ◽  
Annona Muricata ◽  
Randomized Design ◽  
Mortality Index ◽  
Environmentally Safe ◽  
Completely Randomized Design ◽  
Negative Impacts

The increasing of pesticide use is in line with the increasing number of pest populations. However, the use of pesticides causes various negative impacts on the environment (soil, water, and air) such as pesticide-resistant pests, perishing of useful insects which are non-target pesticides, and the use of pesticide which can even lead to poisoning and death in humans. One of the environmentally-safe techniques to control pests is chemical, by the use soursop leaf extract. This study aims to determine the most effective concentration of soursop leaf extract (Annona muricata) in controlling leaf caterpillar (Plutella xylostella) in delima rose apples. The research was carried out in the plantation area of rose apples in tempuran village, Demak. The research method employed experimental research. The study was conducted using Completely Randomized Design. The data were obtained using probit analysis of LC50. The most effective concentration of soursop extract (Annona muricata) on leaf caterpillars’ mortality was 75 ppm. Soursop leaf extract (Annona muricata) has been proven to influence rose apples leaf caterpillars’ mortality index. The LC50 value of soursop leaf extract was 75,16 mg/L. The result showed that soursop leaf extract (Annona muricata) is effective as a biopesticide material to control leaf caterpillar pest for delima rose apples.

scholarly journals AKTIVITAS INSEKTISIDA EKSTRAK TITHONIA DIVERSIFOLIA (HEMSL.) A GRAY (ASTERACEAE) TERHADAP ULAT DAUN KUBIS PLUTELLA XYLOSTELLA (L.) (LEPIDOPTERA: YPONOMEUTIDAE)

2017 ◽  
Vol 17 (2) ◽  
pp. 185
Author(s):  
Efrin Firmansyah ◽  
Dadang . ◽  
Ruli Anwar
Keyword(s):  
Topical Application ◽  
Insecticidal Activity ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Probit Analysis ◽  
Botanical Insecticides ◽  
Tithonia Diversifolia ◽  
Mortality Effect ◽  
Insect Mortality ◽  
Cruciferous Plants

Insecticidal activity of extracts of Tithonia diversifolia (Hemsl.) A. Gray (Asteraceae) against the diamondback moth Plutella xylostella (L.) (Lepidoptera: Yponomeutidae). The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) is one of the most destructive pests on cruciferous plants. Generally farmers use synthetic insecticides to control this pest. Intensive and excessive use of insecticides can cause some undesirable effects such as resistance, resurgence, and contamination of insecticide on environment. One effort to solve the problems caused by synthetic insecticides use is by utilization of botanical materials that are potential to be used as botanical insecticides, one of them is Tithonia diversifolia (Asteraceae). The purpose of this research was to study the insecticidal activity of T. diversifolia as a botanical insecticide against P. xylostella. The leaf residual and topical application methods were used to assess the mortality effect of the extracts. Insect mortality rate was analyzed using probit analysis to obtain LC50 and LC95 values. The results showed that the flower extract had better activity on insect mortality than the leaf extract both in leaf residual and topical application treatments.

scholarly journals Toksisitas ekstrak biji Barringtonia asiatica (Lecythidaceae) terhadap mencit putih (Mus musculus Strain DDY)

Jurnal Agro ◽  
10.15575/3589 ◽  
2018 ◽  
Vol 5 (2) ◽  
pp. 76-85
Author(s):  
Wahyu Daradjat Natawigena ◽  
Danar Dono ◽  
Ivan Febriana
Keyword(s):  
Mus Musculus ◽  
Methanol Extract ◽  
Toxicity Testing ◽  
Probit Analysis ◽  
Seed Extract ◽  
Analysis Method ◽  
Botanical Pesticides ◽  
Weight Level ◽  
Randomized Design ◽  
The Central Nervous System

Salah satu tanaman yang berpotensi sebagai pestisida nabati adalah Bitung (Baringtonia asiatica) yang teruji mengandung terpenoid dan saponin. Penelitian ini bertujuan untuk  mengetahui toksisitas ekstrak kasar (B. asiatica) terhadap mencit putih (Mus musculus) dan potensinya sebagai rodentisida nabati. Penelitian menggunakan metode eksperimen dengan rancangan percobaan Rancangan Acak Lengkap (RAL). Pengujian toksisitas B. asiatica terhadap mencit dilakukan secara oral dengan menghitung LD50 menggunakan metode analisis probit. Pengamatan perilaku mencit yang keracunan ekstrak B.asiatica dilakukan dengan menggunakan metode Wagner & Wolff.  Pengamatan perubahan fisiologis mencit yang teracuni ekstrak metanol biji B. asiatica dilakukan dengan menggunakan kandang metabolisme (Nalgane Metabolic Cages). Hasil penelitian menunjukkan bahwa ekstrak biji B. asiatica bersifat toksik terhadap mencit putih (M. musculus) dengan nilai LD50 = 2022 ppm atau 0,2022% dan digolongkan ke dalam skala toksistas 3 yaitu senyawa dengan toksisitas sedang. Ekstrak B. asiatica mempengaruhi sistem syaraf pusat dan dapat mengakibatkan perubahan pada organ detoksifikasi. Ekstrak biji B. asiatica dapat meningkatkan produksi urin, menurunkan laju konsumsi dan produksi feses, dan menurunkan pertumbuhan bobot mencit. Dengan demikian ekstrak biji B. asiatica berpotensi untuk dikembangkan sebagai bahan aktif rodentisida. One of the plants that potentially as botanical pesticides is the Bitung (Baringtonia asiatica) which is tested contained of terpenoid and saponin. This research aimed was to determine the toxicity of crude extracts (B. asiatica) to white mice (Mus musculus) and their potential as botanical rodenticides. The research used an experimental method with complete randomized design. B. asiatica toxicity testing in mice was carried out orally by calculating LD50 using the probit analysis method. Observation of the behavior of B.asiatica extract poisoning mice was carried out using the Wagner & Wolff method. The physiological experience of mice which were poisoned by the methanol extract of B. asiatica seeds was carried out using a cage (Nalgane Metabolic Cages). The results showed that B. asiatica seed extract was toxic to white mice (M. musculus) with LD50 = 2022 ppm or 0.2022% and classified into toxicity scale 3, namely compounds with moderate toxicity. B. asiatica extract affects the central nervous system and in turn detoxifying organs. B. asiatica seed extract can increase urin production, reduce the rate of consumption and facial production, and reduce the weight level of mice. Therefore that B. asiatica seed extract potentially to be developed as an active ingredients of rodenticides.

scholarly journals Insecticidal selectivity of jayanti plant (Sesbania sesban) for integrated control of diamondback moth (Plutella xylostella)

2017 ◽  
Vol 1 (02) ◽  
pp. 80-84
Author(s):  
SURIPTO SURIPTO ◽  
SUKIMAN SUKIMAN ◽  
ERIN RYANTIN GUNAWAN
Keyword(s):  
Plutella Xylostella ◽  
Integrated Control ◽  
Diamondback Moth ◽  
Water Extract ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Mortality Data ◽  
Sesbania Sesban ◽  
Leaf Extracts ◽  
Randomized Design

Suripto, Sukiman, Gunawan ER. 2017. Insecticidal selectivity of jayanti plant (Sesbania sesban) for integrated control of diamondback moth (Plutella xylostella). Asian J Agric 1: 80-84. It has been known previously that leaves of the jayanti plant (Sesbania sesban (L.) Merr.) containing insecticidal ingredients. This study aimed to evaluate the insecticidal selectivity of various extract fractions of S. sesban leaf for the integrated control of cabbage pest, the diamondback moths (Plutella xylostella). Dried leaf powders from S. sesban were extracted in stages by using hexane, dichloromethane (DCM), ethanol, and water, successively. Each of insecticidal performance of S. sesban leaf extract fractions was tested against two types of test insects, namely P. xylostella larvae as target and Diadegma semiclausum imago as non target insects according to completely randomized design in the cabbage plantation at the village of Sembalun, East Lombok, Indonesia. Each mortality data of P. xylostella larvae and D. semiclausum imago processed by probit analysis to determine the concentration of the death of 50% of test insects (LC50) of each test extract. The results showed that the LC50 of S. sesban leaf extracts classified into four fractions, namely hexane, DCM, and water extract fractions to P. xylostella larvae successively was 343.71, 294.78, 29.95, and 1197.13 ppm, and to D. semiclausum imago row was 305.5, 121.56, 37.38, and 1043.70 ppm. The results showed that the insecticidal activity of S. sesban leaf ethanol extract fraction was selective, because its selectivity value is 1.25. On the other hand each insecticidal performance of three S. sesban leaf extracts, i.e. hexane, DCM, and water extract fractions, respectively is not selective, with the selectivity values are 0.89, 0.41, and 0.87.

scholarly journals Efektivitas Limbah Debu Tembakau sebagai Insektisida Nabati terhadap Ulat Grayak

2019 ◽  
Vol 21 (2) ◽  
pp. 34
Author(s):  
Yumna Aqilah Khairunnisa ◽  
Sholahuddin Sholahuddin ◽  
Ato Sulistyo
Keyword(s):  
Plant Disease ◽  
Probit Analysis ◽  
Botanical Insecticides ◽  
Tobacco Plants ◽  
Botanical Pesticides ◽  
Green House ◽  
Randomized Design ◽  
Phytotoxicity Test ◽  
Completely Randomized Design ◽  
Waste Extract

<p>Botanical pesticides produced from tobacco plants are reported to be the most toxic compared to other types of plants because it contain 2-8% nicotine substances. The objective of this research was to examine the effect of tobacco dust waste extracts and determine the concentration of the most effective tobacco dust waste extract against armyworms. This research was conducted in Laboratory of Pest and Plant Disease and C Green House Faculty of Agriculture Universitas Sebelas Maret Surakarta from April-July 2018. The stages of this study include the multiplication of <em>S. litura </em>test larvae, preparation of botanical insecticides from tobacco dust waste, mortality test, antifeedant test and phytotoxicity test. This study used a completely randomized design with 6 treatments and 4 replications. The results showed that tobacco dust waste was able to increase the mortality of <em>S. litura </em>larvae, but with a slower effect compared to the treatment of profenofos. Feeding barriers are not seen in the test larvae. The phytotoxicity test shows that tobacco dust waste was not toxic to plants. Based on probit analysis, the concentration of tobacco dust waste which was capable of killing 50% of the population of <em>S. litura </em>was equal to 53.10 gL-1.</p>

scholarly journals Bioactivity of microencapsulated soursop seeds extract on Plutella xylostella

2016 ◽  
Vol 46 (5) ◽  
pp. 771-775 ◽  
Author(s):  
Ismael Barros Gomes ◽  
Roseane Cristina Predes Trindade ◽  
Antônio Euzébio Goulart Sant'Ana ◽  
Eurico Eduardo Pinto de Lemos ◽  
Irinaldo Diniz Basílio Júnior
Keyword(s):  
Acute Toxicity ◽  
Plutella Xylostella ◽  
Larval Stage ◽  
Diamondback Moth ◽  
Ethanolic Extract ◽  
Spray Dryer ◽  
Annona Muricata ◽  
Ultrapure Water ◽  
Arabic Gum ◽  
Chronic Effects

ABSTRACT: The aim of this study was to evaluate the bioactivity of microencapsulated extract from the soursop seeds, Annona muricata L. ( Annonaceae ), on diamondback moth, Plutella xylostela L. (Lepidoptera: Plutellidae ). Microencapsulation was performed in a Mini Spray Dryer model B-290 using 50mL of ethanolic and hexanic extracts plus 150mL of ethanol and 150mL of ultrapure water, mixed with aerosil (first polymer) or arabic gum (second polymer). It was possible to microencapsulate the ethanolic extract of soursop seeds only by using the polymer arabic gum at 20%. The microencapsulated extract caused significant acute toxicity (LC50=258mg L-1) and chronic effects, especially reduction of larval viability and increased larval stage. We concluded that the microencapsulation of the ethanolic extract of soursop seeds can be a viable alternative for controlling diamondback moth with possible gains for the environment.

scholarly journals Behavioural responses of diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) to extracts derived from Melia azedarach and Azadirachta indica

2005 ◽  
Vol 95 (5) ◽  
pp. 457-465 ◽  
Author(s):  
D.S. Charleston ◽  
R. Kfir ◽  
L.E.M. Vet ◽  
M. Dicke
Keyword(s):  
Azadirachta Indica ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Feeding Deterrent ◽  
Botanical Insecticides ◽  
Melia Azedarach ◽  
Larval Behaviour ◽  
Control Programmes ◽  
The Impact ◽  
Higher Doses

AbstractThe impact of three different doses of botanical insecticide derived from the syringa tree, Melia azedarach and the neem tree, Azadirachta indica was tested on the behaviour of the diamondback moth, Plutella xylostella (Linnaeus). Both botanical insecticides had a significant impact on larval behaviour. At higher doses the extracts showed feeding deterrent activity, with larvae preferring the untreated sides of cabbage leaves and consuming less of the treated half of cabbage leaves. The botanical insecticides had less of an effect on the oviposition behaviour of P. xylostella moths. In laboratory and glasshouse trials, significantly fewer eggs were oviposited on the plants that had been treated with syringa extracts. Therefore, the syringa extracts appear to have a repellent effect. In contrast, when exposed to the neem extracts the moths did not discriminate between control plants and treated plants. Behavioural observation indicated that, despite the lower number of eggs oviposited on cabbage treated with syringa extracts, the moths chose cabbage treated with the highest dose of syringa more often than they chose control cabbage plants. Similar observations were found in cabbage plants treated with neem, moths chose the medium dose more often than they chose the control. Oviposition and feeding deterrent properties are important factors in pest control, and results from this study indicate that botanical insecticides have the potential to be incorporated into control programmes for P. xylostella in South Africa.

scholarly journals INSECTICIDAL JOINT ACTION OF TERNARY EXTRACT MIXTURES OF FOUR SPECIES OF TROPICAL PLANTS AGAINST Plutella xylostella AND Crocidolomia pavonana

2021 ◽  
Vol 21 (1) ◽  
pp. 34-42
Author(s):  
Djoko Prijono ◽  
Maya Anggraini ◽  
Sri Riski Maryani ◽  
Eliana Diana Surya
Keyword(s):  
Plutella Xylostella ◽  
Joint Action ◽  
Diamondback Moth ◽  
Ternary Mixtures ◽  
Botanical Insecticides ◽  
Vegetable Crops ◽  
Tropical Plants ◽  
Cruciferous Vegetable ◽  
Tropical Plant ◽  
Concentration Ratios

Insecticidal joint action of ternary extract mixtures of four species of tropical plants against Plutella xylostella andCrocidolomia pavonana. The diamondback moth (DBM), Plutella xylostella, and the cabbage head caterpillar (CHC),Crocidolomia pavonana, were two main pests of cruciferous vegetable crops. Among the viable alternative ingredients thatcould be utilized to control those two pests were eco-friendly botanical insecticides. This study was conducted to evaluatethe joint action of ternary extract mixtures of four tropical plant species, i.e. Piper aduncum (Pa), Piper retrofractum (Pr),Sapindus rarak (Sr), and Tephrosia vogelii (Tv), against DBM and CHC. Results of leaf-residue feeding bioassays showedthat based on co-toxicity ratio at both LC 50 and LC levels, ternary mixtures of Pa extract with Sr and Tv extracts at concentrationratios of 1:5:1, 1:5:2, and 2:5:1 indicated synergistic joint action on DBM larvae. A mixture of Pa, Sr and Tv extracts at a ratio of2:5:1 was also synergistic to CHC at both LC level but antagonistic at the LC 95 50 95 and LC 95 levels. This mixture at the 1:5:1 ratio was synergistic on CHC at the LC level whereas at the 1:5:2 ratio was antagonistic to CHC at both LC levels. Furthermore, ternary mixtures of Pr extract with Sr and Tv extracts at the three concentration ratios were synergistic to CHC. Thus, ternary mixtures of Pa or Pr extract with Sr and Tv extracts at appropriate concentration ratios are potential alternatives for the control of DBM and CHC.

scholarly journals Aktivitas Penolak Serangga (Insect Repellent) Ekstrak Klorofom Biji Mimba (Azadirachta Indica) Terhadap Kutu Beras (Calandra Oryzae)

2020 ◽  
Vol 1 (2) ◽  
pp. 55-63
Author(s):  
Isma Oktadiana ◽  
Venny Diah Ningsih
Keyword(s):  
Azadirachta Indica ◽  
Seed Extract ◽  
Botanical Insecticides ◽  
Insect Repellent ◽  
Neem Seed ◽  
Application Time ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Control ◽  
Neem Seed Extract

Plants that are currently being developed as botanical insecticides are those that produce essential oils. The largest active compound that is insecticidal in neem (Azadirachta indica) is azadirachtin, this compound belongs to the triterpenoid group. The purpose of this study was to determine the level of insect repellent activity of neem (Azadirachta indica) seed chlorophome extract against rice lice (Calandra oryzae). This research is an experimental study using a completely randomized design (CRD). Consisting of neem seed extract treatment with a concentration of 0.1% and 0.5% and a control in the form of distilled water each 10 times. The results of this study indicated that the concentration of 0.1% and 0.5% neem seed extract test increased the mortality of rice lice. The longer the application time, the higher the mortality rate of rice lice. In the treatment of 0.5% neem seed extract, it could cause 67% mortality compared to 0.1% neem seed extract treatment and control.   Keywords: Neem seeds, insect repellent, Calandra oryzae ABSTRAK   Tumbuhan yang saat ini sedang dikembangkan sebagai insektisida nabati yaitu tumbuhan yang menghasilkan minyak atsiri. Senyawa aktif terbesar yang bersifat insektisida pada tanaman mimba (Azadirachta indica) adalah azadirachtin, senyawa ini termasuk dalam kelompok triterpenoid. Tujuan dari penelitian ini yaitu untuk mengetahui tingkat aktivitas penolak serangga (insect repellent) ekstrak klorofom biji mimba (Azadirachta indica) terhadap kutu beras (Calandra oryzae). Penelitian ini merupakan penelitian eksperimental yang menggunakan Rancangan Acak Lengkap (RAL). Terdiri atas perlakuan ekstrak biji mimba konsentrasi 0.1% dan 0.5 % dan kontrol berupa akuades masing-masing 10 kali ulangan. Hasil penelitian ini menunjukkan bahwa Uji ekstrak biji mimba konsentrasi 0.1% dan 0.5% meningkatkan mortalitas kutu beras. Semakin lama waktu aplikasi maka semakin tinggi tingkat mortalitas kutu beras. Pada perlakuan  ekstrak biji mimba 0.5% dapat menyebabkan kematian sebesar 67% dibandingkan dengan perlakuan ekstrak biji mimba 0.1% dan kontrol.   Kata Kunci: Biji Nimba, penolak serangga, Calandra oryzae

scholarly journals Pemberian Ekstrak Biji Alpukat (Persea americana Mill.) dan Biji Sirsak (Annona muricata L.) terhadap Mortalitas Larva Nyamuk Culex Sp

2017 ◽  
Vol 10 (02) ◽  
pp. 1-15
Author(s):  
S. Nombe ◽  
Diah Karunia Binawati
Keyword(s):  
Hemorrhagic Fever ◽  
Seed Extract ◽  
Persea Americana ◽  
Natural Material ◽  
Mosquito Larvae ◽  
Annona Muricata ◽  
Randomized Design ◽  
And Control ◽  
Culex Sp ◽  
Avocado Seed

Vegetable insecticide is a natural material that is environmentally friendly. This material can be used in the prevention and control of mosquito larvae, especially Culex sp. Culex sp is the main vector of filariasis disease and dengue hemorrhagic fever (DHF). Use of natural materials is very good and environment friendly as insectcide compared with chemicals. Avocado seed extract (Persea americana Mill) is known to have secondary metabolite content of alkaloids, saponins, triterpenoids while soursop extract (Annona muricata L) in the form of acetogenin, squamocin and annonacin. This research needs to use of avocado seed extract (P. americana Mill) and soursop seed extract (A. muricata L) as larvacide of Culex sp. The aim of this research is to know the effect of avocado seed extract (P. americana Mill) and soursop seed extract (A. muricata L) to mortality of Culex sp Instar III mosquito larvae and LC50 for 24 hours. This study was an experimental study using a complete randomized design (RAL) with five treatment concentrations (0, 2, 4, 6, and 8%) avocado seed extract (P. americana Mill) and soursop extracts extract (A. muricata L) as much as five repetitions. The data obtained were analyzed by using One Way ANOVA to show the effect on the treatment. The results showed that giving of avocado seed extract (P. americana Mill) with 8% concentration (23,8 ± 0,45 larvae) was optimal in killing Culex sp mosquito larvae. While giving soursop seed extract (A. muricata L) with 4% concentration (25 ± 0,0 larva) optimal in killing Culex sp mosquito larvae. Avocado seed extract (P. americana Mill) has an effect as a larvacide against Culex sp. instar III with LC50 at concentration 2,486% while soursop extract (A. muricata L) with LC50 value at concentration 1,605%.   Keywords: Larvacide, soursop seed extract, avocado seed extract, Culex sp

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