scholarly journals The Effects of a Zinc-Methionine Supplementation in High Producing Dairy Cows

2021 ◽  
Author(s):  
Cori Siberski ◽  
James E. Koltes ◽  
Janaka Wickramasinghe ◽  
Maria Jose Oconitrillo ◽  
Nadiia Stepanchenko ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Zinc Methionine ◽  
Methionine Supplementation

38 Developmental competence of bovine cumulus–oocyte complexes collected from cows fed rumen-protected methionine and lysine

2021 ◽  
Vol 33 (2) ◽  
pp. 126
Author(s):  
M. Ritz ◽  
A. Gonzalez ◽  
A.-S. Fries ◽  
T. Scheu ◽  
N. Blad-Stahl ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Follicular Fluid ◽  
Oocyte Quality ◽  
Blastocyst Stage ◽  
Developmental Competence ◽  
Isopropyl Ester ◽  
Lactating Dairy Cows ◽  
Endogenous Lipid ◽  
Developmental Rates ◽  
Methionine Supplementation

Supplementation of rumen-protected amino acids (RPAA) has proven to be an effective tool to supply limiting AA in dairy diets. Methionine and lysine are the two most limiting AA for lactating dairy cows. Recently, it has been shown that methionine supplementation seems to affect pre-implantation embryos collected from superovulated cows enhancing their developmental competence because there is strong evidence that endogenous lipid reserves serve as an energy substrate (Acosta et al. 2016 Theriogenology 85, 1669–1679). Moreover, higher concentrations of methionine were determined in the follicular fluid of the first dominant follicle postpartum in cows supplemented with rumen-protected methionine and rumen-protected choline from 21 days before calving to 30 days postpartum and it was assumed that higher methionine concentrations in the follicular fluid could affect oocyte quality (Acosta et al. 2017 Theriogenology 96, 1–9). There is no information available so far regarding the effect of a combined methionine and lysine supplementation (each rumen-protected) on oocyte quality. Therefore, the objective of this study was to evaluate the effect of a combined methionine and lysine supplementation during early to mid-lactation on the developmental competence of oocytes collected from lactating dairy cows (days 0 to 100 p.p.). Thirty pregnant multiparous German Holstein dairy cows were grouped 3 weeks before their expected calving date, receiving identical diets. After calving, they were randomly allocated to 2 groups fed a total mixed ration supplemented with (N=14 cows; RPAA) or without (N=16 cows; CON) LysiGEMTM (encapsulated lysine; Kemin Industries) and Metasmart DryTM (isopropyl ester of the hydroxylated analogue of methionine adsorbed onto a silicon dioxide carrier; Adisseo). Starting from 45 days p.p., animals from both groups were submitted to an ovum pickup (OPU) session once a week for at least 8 weeks. Collected cumulus–oocyte complexes (COC) were subjected to a standard invitro production (IVP) protocol (Stinshoff et al. 2014 Reprod. Fertil. Dev. 26, 502–10) including IVM, IVF, and invitro culture (IVC). Cleavage and developmental rates up to the morula/blastocyst stage were recorded on Days 3, 7, and 8. In total, 1211 follicles have been aspirated from RPAA animals compared with 1413 from CON animals, from which 742 and 885 COC were collected, respectively. The calculated recovery rate based on the number of aspirated follicles and collected COC was similar for both groups (61.3±29.4% vs. 62.6±33.5%). Cleavage and developmental rates based on 240 (RPAA group) and 299 (CON group) COC also showed similar results [RPAA: 84.1±5.9% (202/240), 18.3±4.4% (44/240), 18.8±4.7% (45/240); CON: 81.9±8.6% (245/299), 15.4±8.9% (46/299), 16.7±8.4% (50/299)]. In conclusion, supplementation of RPAA (methionine and lysine) had no beneficial effect on the developmental competence of COC obtained from these animals compared with those collected from cows fed the diet without RPAA supplementation.

scholarly journals Effect of N-acetyl-l-methionine supplementation on lactation performance and plasma variables in mid-lactating dairy cows

2019 ◽  
Vol 102 (6) ◽  
pp. 5182-5190 ◽  
Author(s):  
S.L. Liang ◽  
Z.H. Wei ◽  
J.J. Wu ◽  
X.L. Dong ◽  
J.X. Liu ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Lactation Performance ◽  
Lactating Dairy Cows ◽  
Methionine Supplementation

The effects of methionine supplementation upon milk composition and production of forage-fed dairy cows

1999 ◽  
Vol 79 (2) ◽  
pp. 235-241 ◽  
Author(s):  
D. Pacheco-Rios ◽  
W. C. McNabb ◽  
J. P. Hill ◽  
T. N. Barry ◽  
D. D. S. Mackenzie
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Milk Protein ◽  
Whey Proteins ◽  
Protein Composition ◽  
Milk Composition ◽  
Metabolizable Energy ◽  
Protein Nitrogen ◽  
Methionine Supplementation ◽  
And Control

Two experiments (mid- and late lactation) were conducted to test whether methionine (Met) limited milk production in Friesian cows fed sole diets of fresh perennial ryegrass–white clover pasture. In mid-lactation (exp. 1), 26 cows were assigned to one of three treatments: ruminally protected oral DL-Met (15 g d−1), continuous intrajugular infusion of L-Met (15 g d–1) and control. Twelve animals from exp. 1 were used during late lactation (exp. 2) and distributed in two experimental groups: ruminally protected oral DL-Met (15 g d−1) and control. Dry matter, metabolizable energy, crude protein and Met intakes, milk yield and composition and blood Met, cysteine and urea were measured. Oral and intravenous Met supplementation increased blood Met concentration by 50–90% compared with controls. Met supplementation did not alter the concentration of milk fat, protein or lactose in either experiment. Met supplementation had no significant effects on yields of fat, lactose, casein, whey proteins or non-protein nitrogen during mid-lactation. In late lactation, Met supplementation did not affect milk protein composition or yield of milk components, with exception of a decrease (P < 0.05) in the yield of β -casein. Intravenous Met supplementation increased (P < 0.05) the efficiency of conversion of pasture N to milk protein in mid-lactation. Key words: Dairy cows, methionine, ruminally protected methionine, milk protein, casein, fresh pasture diets

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