scholarly journals Biological activities and phytochemical analysis of extracts Afrostyrax lepidophyllus Mildbr. seeds

2017 ◽  
Vol 6 (2) ◽  
pp. 102-106
Author(s):  
Namkona Armel Frédéric ◽  
◽  
Bolevane Ouantinam Serge Florent ◽  
Moustapha Fatia ◽  
Worowounga Xavier ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Dry Matter ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Phytochemical Analysis ◽  
Tannin Content ◽  
Weak Correlation ◽  
Polyphenol Content ◽  
Human Diet

Oxidative stress, one of the causes of certain diseases, prompts the search for new antioxidant molecules to reduce or limit the damage caused by it. In this study, the antioxidant, anti-inflammatory and anti-xanthine oxidase activity of the extracts (cyclohexane, dichloromethane, ethyl acetate and methanol) of the Afrostyrax lepidophillus seeds used in the human diet and the Central African Pharmacopoeia Been measured. Total pholyphenols, flavonoids, tannins and anthocyanins were also quantified in this work .The IC50 (inhibitory concentration which reduces free radicals by 50%) of the antioxidant activity of their extracts are respectively of the order of 3890,5±4,3, 3313,8±3,5, 1872,8±1,0 et 248,4±0,1 mg/L. The phytochemical analysis of the various extracts (cyclohexane, dichloromethane, ethyl acetate and methanol) made it possible to quantify the polyphenol content of 13,1±0,4, 30,5±0,5, 51,8±1,6 et 33,2±4,0 mg eq AG/g Dry matter (MS). The tannin content is of the order of 2,35±0,3, 10,68±0,1, 7,78±0,2, mg eq Cat/g MS, That of anthocyanins is 0.79 ± 0.04, 0.65 ± 0.02, 1.65 ± 0.07 and 0.18 ± 0.03 mg eq C3GE / g MS. The analysis of the different correlations between the chemical families and the antioxidant activity showed that there is a weak correlation between the biological activity and the phenolic compounds.

The Antioxidant Activity and Phytochemical Analysis of Medicinal Plant Veronica Biloba

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
AMIR HASSAN ◽  
Himayat Ullah ◽  
Muhammad Israr
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Continuous Process ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Analysis ◽  
Uniform Size ◽  
Health Related

A medicinal plant veronica genus has 450 well known species and found across both temperate and hemisphere region their 26 species are endemic and known in a total of 79 popular species and are widely utilized throughout the world due to important biological activities. In this study fully powdered uniform size specie veronica biloba plant taken in porous bag were manually subjected to soxhlet hot continuous process for cyclization of extraction using ethanol (300 mL) a concentrated dried extract obtained after solvent evaporation. Furthermore, liquid-liquid extracted fractions as water, dichloromethane, n-hexane, and ethyl acetate yields results founds polar fraction with highest percentage (water 47.51 %). The phytochemical screening of veronica biloba has shown all major compounds entirely present in extracts. One of the primitive phenolic compound flavonoid is present in plant and show potency towards antioxidants. All the extracted fractions of plant showed excellent antioxidant activity using a stable DPPH (1,1-diphenyl-2-picryl-hydrazyl) method at concentration (range from 31.25 to 500µg/mL). Primary our ethyl acetate extract fraction showed highest inhibition potential at IC50 = 1.70±0.05µg/mL which is much closer to a standard positive control Propyl gallate showed IC50 = 1.6±0.05µg/mL percent potential. The purification and isolation of these extract is important which can provide us help in novel antioxidants discovery also natural antioxidants currently in cosmetics products, food and therapeutics health related products significantly demanded because they are very effective, efficient and harmless as compared to synthetic one.

scholarly journals Phytochemical Content, Antibacterial and Antioxidant Potential of Endemic Plant Anabasis aretioïdes Coss. & Moq. (Chenopodiaceae)

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Souad Senhaji ◽  
Fatima Lamchouri ◽  
Hamid Toufik
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Endemic Plant

This study aims to investigate the biological activities of endemic plant Anabasis aretioïdes harvested in eastern Morocco. Various extracts were prepared from aerial part by aqueous and organic extraction using a Soxhlet and cold maceration. Preliminary phytochemical analysis was carried out on the powder and on the different extracts by standard phytochemical tests, and was confirmed by a quantitative analysis based on the determination of total polyphenols and cathechic tannins. Antioxidant activity was evaluated in vitro by five methods: H2O2, DPPH, ABTS, FRAP, and RP, and the antibacterial activity was carried out by disc diffusion method and the determination of MIC and MBC. Phytochemical screening revealed the presence of cathechic tannins, saponins, and sterols and quantitative analysis showed that Ethyl acetate extract presented the high level of phenolic and cathechic tannins contents (46.79 ± 0.75 µg GAE/mg E and 46.46 ± 0.67 µg CE/mg E). A highest hydrogen peroxide activity was observed in aqueous macerated extract (7.84 ± 0.44%) and the macerated methanol extract has the highest rates for the other four antioxidant activity tests: It was able to reduce DPPH with an IC50 of 52.91 ± 0.24 µg/ml, the highest ABTS•+ radical scavenging capacity (48.99 ± 1.316 µg TE/mg E), it showed also the highest antioxidant activity by the FRAP and reducing power test (99.73 ± 3.570 µg TE/mg E and 72.176 ± 0.540 µg AAE/mg E). Antibacterial screening showed that the maximum zone of inhibition was noted for ethyl acetate extract against Staphylococcus aureus (13.5 mm). The lowest MIC value was obtained with methanolic and macerated methanolic extracts against Protéus mirabilis strain (MIC = 3.125 mg/ml). Principal component analysis showed that the four methods ABTS, DPPH, FRAP, and RP are highly correlated and a correlation between the antioxidant activity and the total phenolic contents of the extracts indicated that phenolic compounds were the dominant contributors to the antioxidant activity of the plant.

scholarly journals The antioxidant activity and phytochemical analysis of medicinal plant Veronica biloba

2019 ◽  
Vol 8 (4) ◽  
pp. 732-738
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Continuous Process ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Analysis ◽  
Uniform Size ◽  
Health Related

A medicinal plant veronica genus has 450 well known species and found across both temperate and hemisphere region their 26 species are endemic and known in a total of 79 popular species and are widely utilized throughout the world due to important biological activities. In this study fully powdered uniform size specie veronica biloba plant taken in porous bag were manually subjected to soxhlet hot continuous process for cyclization of extraction using ethanol (300 mL) a concentrated dried extract obtained after solvent evaporation. Furthermore, liquid-liquid extracted fractions as water, dichloromethane, n-hexane, and ethyl acetate yields results found polar fraction with the highest percentage (water 47.51 %). The phytochemical screening of veronica biloba has shown all major compounds entirely present in extracts. One of the primitive phenolic compound flavonoid is present in plant and show potency towards antioxidants. All the extracted fractions of plant showed excellent antioxidant activity using a stable DPPH (1,1-diphenyl-2-picryl-hydrazyl) method at concentration (range from 31.25 to 500µg/mL). Primary our ethyl acetate extract fraction showed the highest inhibition potential at IC50 = 1.70±0.05µg/mL which is much closer to a standard positive control Propyl gallate showed IC50 = 1.6±0.05µg/mL percent potential. The purification and isolation of these extract areimportant which can provide us help in novel antioxidants discovery also natural antioxidants currently in cosmetics products, food and therapeutics health related products significantly demanded because they are very effective, efficient and harmless as compared to synthetic.

scholarly journals Antioxidant Activity in Rheum emodi Wall (Himalayan Rhubarb)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2555
Author(s):  
Sang Koo Park ◽  
Yoon Kyung Lee
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Polyphenol Content ◽  
Abts Assay ◽  
Ic50 Values

Using natural products as antioxidant agents has been beneficial to replace synthetic products. Efforts have been made to profile the antioxidant capacities of natural resources, such as medicinal plants. The polyphenol content of Himalayan rhubarb, Rheum emodi wall, was measured and the antioxidant activity was determined using DPPH and ABTS+ assay, and the oxidative stress was assessed using SOD enzymatic assay. Five different solvent fractions, n-hexane, n-butanol, ethyl acetate, dichloromethane, and water, were used for screening the antioxidant capacity in effort to determine the optimum extraction solvent. The total phenolic contents for R. emodi fractions ranged from 27.76 to 209.21 mg of gallic acid equivalents (GAE)/g of dry weight. DPPH and ABTS+ assay results are presented into IC50 values, ranged from 21.52 to 2448.79 μg/mL and 90.25 to 1718.05 μg/mL, respectively. The ethyl acetate fraction had the highest antioxidant activity among other fractions. Also, n-butanol and water fractions showed significantly lower IC50 values than the positive control in DPPH radical scavenging activity. The IC50 values of SOD assay of fractions ranged from 2.31 to 64.78 μg/mL. A similar result was observed with ethyl acetate fraction showing the highest SOD radical scavenging activity. The study suggests that the ethyl acetate fraction of R. emodi possess the strongest antioxidant activity, thus the most efficient in extracting antioxidant contents. Moreover, a highly significant correlation was shown between total polyphenol content and antioxidant activity screening assays. The compounds related to the antioxidant activity of R. emodi were identified to myricitrin, myricetin 3-galloyl rhamnoside, and myricetin, which have not been reported in studies about R. emodi before.

scholarly journals Study and evaluation of antimicrobial activity and antioxidant capacity of dry extract and fractions of leaves of Raphanus sativus var. oleiferus Metzg.

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Ana Flávia da Silva ◽  
Marisa de Oliveira Lopes ◽  
Cláudio Daniel Cerdeira ◽  
Ingridy Simone Ribeiro ◽  
Isael Aparecido Rosa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Minimum Inhibitory Concentration ◽  
Ethyl Acetate ◽  
Raphanus Sativus ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Fraction ◽  
Antimicrobial Activities ◽  
Dry Extract ◽  
Antioxidant And Antimicrobial Activities

The radish (Raphanus sativus L.) is a vegetable of the Brassicaceae family cultivated worldwide and has several medicinal properties. Its biological activities are related to various secondary metabolites present in the species, especially phenolics. Thus, the objectives of this study were the chemical analysis and evaluation of the antioxidant and antimicrobial activities of the dry extract and fractions of the fodder turnip leaves (R. sativus var. oleiferus Metzg.). Samples were analyzed by mass spectrometry and the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical method and the reducing power method. Antimicrobial activity was determined by the agar diffusion and microdilution methods. The total phenols were concentrated in the butanol fraction (121.27 mg GAE/g) and the flavonoids were concentrated in the ethyl acetate fraction (98.02 mg EQ/g). The ethyl acetate fraction showed the best antioxidants results, with 83.45% of free radical scavenging and 11.34% of ferric ions reduction. The analysis of antimicrobial activity showed that the dry extract had the highest average zone of inhibition against Bacillus subtilis (18.67 mm). Smaller values of the minimum inhibitory concentration for Micrococcus luteus were, and the ethyl acetate fraction showed a lower minimum inhibitory concentration (0.1 mg/ml) for that microorganism. There was a strong correlation between the antioxidant activity and the content of phenols and flavonoids. The results showed the potential antioxidant and antimicrobial activities of this extract with the ethyl acetate fraction being most promising for further studies.

scholarly journals Antibacterial and Antioxidant Activities of Slender Amaranth Weed

2020 ◽  
Vol 38 ◽  
Author(s):  
M. AKBAR ◽  
I.N. SHERAZI ◽  
M.S. IQBAL ◽  
T. KHALIL ◽  
H.M. WAQAS
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pseudomonas Syringae ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Root Extract

ABSTRACT: In the present study, antibacterial and antioxidant [1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity] activities of a weed, slender amaranth (Amaranthus viridis L.) were investigated. Extracts of different plant parts were prepared in n-hexane, chloroform and ethyl acetate. Antibacterial activity was measured by using 100 mg mL-1 concentration extracts against 4 deadly phytopathogenic bacterial species viz. Pseudomonas syringae Van Hall, Ralstonia solanacearum Smith, Erwinia carotovora (Jones), Holland and Xanthomonas axonopodis Hasse. In antioxidants assays, 10, 20 and 30 mg mL-1 extracts were used keeping DPPH as control. In these bioassays, ethyl acetate fraction of A. viridis leaf exhibited the best antibacterial and antioxidants activity. Ethyl acetate leaf fraction showed the highest inhibition zone diameter (IZD) where it caused 21 mm IZD against P. syringae and 19 mm IZD against E. carotovora. This extract also showed 22, 52 and 84% antioxidant activity at 10, 20 and 30 mg mL-1 concentrations, respectively. Previously there is no report available that describes antibacterial activity of root extract of A. viridis against P. syringae. Moreover, antioxidant activity of stem and root extracts in n-hexane, chloroform and ethyl acetate was investigated first time in the world. It was concluded that the biological activities observed during the present investigation may be due to the presence of bioactive constituents that can be harnessed as natural antibacterials and antioxidants.

scholarly journals Antioxidant Activity, Total Polyphenol, Flavonoid and Tannin Contents of Fermented Green Coffee Beans with Selected Yeasts

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 29 ◽  
Author(s):  
Mesfin Haile ◽  
Won Kang
Keyword(s):  
Antioxidant Activity ◽  
Green Coffee ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Fermentation Time ◽  
Green Coffee Beans ◽  
Coffee Beans ◽  
The Mean

We examined the antioxidant activity, total polyphenol content (TPC), total flavonoid content (TFC), total tannin content (TTC) and physical characteristics of green coffee beans fermented with selected yeasts. There was no significant (p > 0.05) interaction effect between yeast-fermented coffee extracts and duration of fermentation on antioxidant activity (oxygen radical absorbance capacity [ORAC] and superoxide dismutase-like [SOD-like] activity). However, the mean of the antioxidant activity (ORAC and SOD-like activity) significantly (p < 0.05) increased in the fermented coffee extracts compared to unfermented coffee. There were significant (p < 0.05) interaction effects between yeast-fermented coffee extracts and duration of fermentation (24 h and 48 h) on the TPC, TFC, TTC and pH of the fermented solution and on the colors of the ground-roasted coffee. The TPC showed a pattern of increase in samples Ferm-1 and Ferm-3 as fermentation time increased from 24 h to 48 h. However, a decreasing TPC trend was observed in Ferm-2 as the number of fermentation hours increased from 24 to 48. The fermented coffee beans had a significantly higher flavonoid content than the unfermented coffee beans, while fermentation significantly decreased the tannin content compared to that in unfermented coffee.

scholarly journals ANTIOXIDANT ACTIVITY, PHYTOCHEMICAL ANALYSIS AND TOTAL POLYPHENOLICS CONTENT OF ESSENTIAL OIL, METHANOL EXTRACT AND METHANOL FRACTIONS FROM COMMELINA NUDIFLORA

2018 ◽  
Vol 10 (8) ◽  
pp. 36 ◽  
Author(s):  
Muhammad Dawood Shah ◽  
Mohammad Iqbal
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Ethyl Acetate ◽  
Palmitic Acid ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Hexadecanoic Acid ◽  
Methanol Extracts

Objective: In the present study, the essential oil, methanol extract, and methanol fractions (n-hexane, chloroform, ethyl acetate, and n-butanol) obtained from Commelina nudiflora were investigated for the free radical scavenging effects and phytochemical analysis.Methods: The antioxidative effect of the essential oil, methanol extracts and methanol fractions were evaluated using 2, 2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau and aluminium chloride reagents respectively. The phytochemical analyses of the essential oil, methanol extracts and methanol fractions were performed by gas chromatography and mass spectrometry (GCMS). Results: The antioxidant, total phenolic and total flavonoid contents of butanol, ethyl acetate and chloroform fractions were higher followed by methanol extract, hexane fraction and essential oil. Phytochemical analysis indicated the presence of alkaloid, saponin, steroid, phytosterols, triterpenoids and tannins etc. The identified bioactive constituents of essential oil, methanol extract and methanol fractions of C. nudiflora were indole, 2-methoxy-4-vinylphenol, 2-pentadecanone, 6,10,14-trimethyl, phenol, benzyl alcohol, eugenol, phenol, 2, 4-bis (1,1-dimethylethyl), hexadecanoic acid, ethyl ester (palmitic acid ester), n-hexadecanoic acid (palmitic acid), 9, 12-octadecadienoic acid, (linoleic acid) and phytol. All identified bioactive compounds and their derivatives were generally reported with antimicrobial, antioxidant, anti-inflammatory and antitumor properties.Conclusion: The obtained data suggest that the essential oil, methanol extract and methanol fractions of C. nudiflora possess remarkable antioxidant activities and vital phytochemicals. Thus the plant can be a utilized as a potential source of nutraceutical with antioxidant activity.

scholarly journals Natural Phytochemicals and Biological Activities of Wild Grape (Ampelocissus martinii Planch.) Root Extract

2021 ◽  
Vol 33 (3) ◽  
pp. 545-550
Author(s):  
P. Siripipatthana ◽  
P. Srihanam ◽  
A. Sangdee
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antibacterial Activities ◽  
Root Extract ◽  
Phytochemical Content ◽  
Gram Positive Bacteria ◽  
High Antioxidant Activity

A hydromethanolic root extract of Ampelocissus martinii Planch. (A. martinii) was analyzed by standard methods for its phytochemical content, antioxidant activity, α-amylase and α-glucosidase inhibitions and antibacterial activities. The root extract exhibited the highest content of saponins, followed by phenols, proanthocyanidin and flavonoids, respectively. It showed high antioxidant activity in FRAP and CUPRAC assays. The root extract and standard Trolox had similar antioxidant activities in the DPPH and ABTS assay. It also showed much higher α-amylase and α-glucosidase inhibitory activity compared to standard acarbose. Moreover, the root extract inhibited all tested Gram-positive bacteria with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 6.25 mg/mL. These results indicate that A. martinii root can be pharmaceutically used as active ingredients to prevent bacterial infection and radical-related diseases especially diabetes.

scholarly journals Phytochemical analysis and antioxidant activity of different plant parts of Pellacalyx axillaris

2019 ◽  
Vol 15 (3) ◽  
pp. 394-397
Author(s):  
Najwa Ahmad Kuthi ◽  
Norazah Basar
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Plant Parts ◽  
Crude Extracts ◽  
Radical Scavenging Assay

Pellacalyx axillaris or locally known as ‘membuloh’ is a mangrove species belonging to the Rhizophoraceae family. Till date, there has been only one phytochemical study found on this particular plant species and that without any documentation on its biological activities. Therefore, the present work aimed to reveal the phytoconstituents and the antioxidant activity of different crude extracts from different plant parts of P. axillaris. Experimentally, three organic solvents of different polarities i.e. n-hexane, ethyl acetate and methanol were used to prepare the crude extracts from the dried leaves, twigs and barks of P. axillaris. The preliminary phytochemical screening of this species indicated the presence of terpenoids, phenolic compounds, tannins, flavonoids, alkaloids, anthraquinone glycosides and carbohydrates. The in vitro antioxidant activity of the species evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay, and ferric reducing antioxidant power (FRAP) suggested that the methanolic bark extract contained potential source of natural antioxidants. Further research into isolation of antioxidant compounds from this species is highly recommended.

Sign in / Sign up

Export Citation Format

Share Document