DIN EN 15634-4:2021-11, Lebensmittel_- Nachweis von Lebensmittelallergenen mit molekularbiologischen Verfahren_- Teil_4: Erdnuss (Arachis hypogaea)_- Qualitativer Nachweis einer spezifischen DNA-Sequenz in Schokolade mittels Real-time PCR; Deutsche und Englische Fassung prEN_15634-4:2021

2021 ◽  
Keyword(s):  
Real Time ◽  
Arachis Hypogaea ◽  
Real Time Pcr

Development of a real-time PCR genotyping assay to identify high oleic acid peanuts (Arachis hypogaea L.)

2009 ◽  
Vol 25 (3) ◽  
pp. 541-548 ◽  
Author(s):  
Noelle A. Barkley ◽  
Kelly D. Chenault Chamberlin ◽  
Ming Li Wang ◽  
Roy N. Pittman
Keyword(s):  
Oleic Acid ◽  
Real Time ◽  
Arachis Hypogaea ◽  
Real Time Pcr ◽  
High Oleic Acid ◽  
High Oleic ◽  
Genotyping Assay ◽  
Arachis Hypogaea L ◽  
Pcr Genotyping

Development of a real time PCR assay for detection of allergenic trace amounts of peanut (Arachis hypogaea) in processed foods

Food Control ◽  
2013 ◽  
Vol 30 (2) ◽  
pp. 480-490 ◽  
Author(s):  
Inés María López-Calleja ◽  
Silvia de la Cruz ◽  
Nicolette Pegels ◽  
Isabel González ◽  
Teresa García ◽  
...  
Keyword(s):  
Real Time ◽  
Arachis Hypogaea ◽  
Real Time Pcr ◽  
Processed Foods ◽  
Pcr Assay

scholarly journals Identification of Transcripts Involved in Resistance Responses to Leaf Spot Disease Caused by Cercosporidium personatum in Peanut (Arachis hypogaea)

2005 ◽  
Vol 95 (4) ◽  
pp. 381-387 ◽  
Author(s):  
M. Luo ◽  
P. Dang ◽  
M. G. Bausher ◽  
C. C. Holbrook ◽  
R. D. Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Resistance ◽  
Real Time ◽  
Arachis Hypogaea ◽  
Real Time Pcr ◽  
Leaf Spot ◽  
Leaf Spot Disease ◽  
Disease Resistance Gene ◽  
Cercosporidium Personatum ◽  
Spot Disease

Late leaf spot disease caused by Cercosporidium personatum is one of the most destructive foliar diseases of peanut (Arachis hypogaea) worldwide. The objective of this research was to identify resistance genes in response to leaf spot disease using microarray and real-time polymerase chain reaction (PCR). To identify transcripts involved in disease resistance, we studied the gene expression profiles in two peanut genotypes, resistant or susceptible to leaf spot disease, using cDNA microarray containing 384 unigenes selected from two expressed sequenced tag (EST) cDNA libraries challenged by abiotic and biotic stresses. A total of 112 spots representing 56 genes in several functional categories were detected as up-regulated genes (log2 ratio > 1). Seventeen of the top 20 genes, each matching gene with known function in GenBank, were selected for validation of their expression levels using real-time PCR. The two peanut genotypes were also used to study the functional analysis of these genes and the possible link of these genes to the disease resistance trait. Microarray technology and real-time PCR were used for comparison of gene expression. The selected genes identified by microarray analysis were validated by real-time PCR. These genes were more greatly expressed in the resistant genotype as a result of response to the challenge of C. personatum than in the susceptible genotype. Further investigations are needed to characterize each of these genes in disease resistance. Gene probes could then be developed for application in breeding programs for marker-assisted selection.

Feldvalidierung einer real-time PCR zum Nachweis von Mycoplasma hyopneumoniae in Nasentupfermaterial von lebenden Schweinen

2005 ◽  
Vol 147 (9) ◽  
pp. 373-379 ◽  
Author(s):  
F. Zeeh ◽  
P. Kuhnert ◽  
R. Miserez ◽  
M. G. Doherr ◽  
W. Zimmermann
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mycoplasma Hyopneumoniae
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