Development of a Real-Time PCR and a Sandwich ELISA for Detection of Potentially Allergenic Trace Amounts of Peanut (Arachis hypogaea) in Processed Foods

2004 ◽  
Vol 52 (12) ◽  
pp. 3754-3760 ◽  
Author(s):  
Oliver Stephan ◽  
Stefan Vieths
Keyword(s):  
Real Time ◽  
Arachis Hypogaea ◽  
Real Time Pcr ◽  
Sandwich Elisa ◽  
Processed Foods

Development of a real time PCR assay for detection of allergenic trace amounts of peanut (Arachis hypogaea) in processed foods

Food Control ◽  
2013 ◽  
Vol 30 (2) ◽  
pp. 480-490 ◽  
Author(s):  
Inés María López-Calleja ◽  
Silvia de la Cruz ◽  
Nicolette Pegels ◽  
Isabel González ◽  
Teresa García ◽  
...  
Keyword(s):  
Real Time ◽  
Arachis Hypogaea ◽  
Real Time Pcr ◽  
Processed Foods ◽  
Pcr Assay

Detection of transgenic rice line TT51-1 in processed foods using conventional PCR, real-time PCR, and droplet digital PCR

Food Control ◽  
2019 ◽  
Vol 98 ◽  
pp. 380-388 ◽  
Author(s):  
Xiaofu Wang ◽  
Ting Tang ◽  
Qingmei Miao ◽  
Shilong Xie ◽  
Xiaoyun Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Transgenic Rice ◽  
Real Time Pcr ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Processed Foods ◽  
Conventional Pcr ◽  
Rice Line ◽  
Transgenic Rice Line

Molecular epidemiology and phylogenetic analysis of foot and mouth disease virus type ‘O’ and evaluation of its carrier state in cattle of Assam by Real time PCR

2018 ◽  
Author(s):  
Sangeeta Baro ◽  
Krishna Sharma ◽  
Biswajyoti Sharma ◽  
Shantanu Tamuly ◽  
P. Deka ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Sandwich Elisa ◽  
Foot And Mouth Disease ◽  
Carrier State ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

The molecular epidemiological study of foot-and-mouth disease virus (FMDV) has been carried out from different outbreaks in Assam the present study is based on the nucleotide sequencingof circulating FMDV serotype. The samples were subjected to sandwich ELISA, multiplex-PCR and molecular phylogeny to identify the type species. The phylogenetic analysis of virus sequence revealed similarity with theBangladesh isolates in the major branching pattern. The serotype ‘O’has found to be dominant and responsible for most of the recentoutbreaks.Thepersistence of serotype ‘O’ and cytokines expression of IL-1á, IL-1â, IFN-á, TNF-á in blood of recovered animals were done by Real time PCR. The findings indicated that IL-1á, IFN-á and TNF-á genes were up-regulated upto 3 months post infection but IL-1â found to be down regulated with progression of recovery. The present study thus supports that real-time PCR is a powerful technique for reliable detection of persistent FMDV in recovered animals.

scholarly journals Highly Sensitive GMO Detection Using Real-Time PCR with a Large Amount of DNA Template: Single-Laboratory Validation

2018 ◽  
Vol 101 (2) ◽  
pp. 507-514 ◽  
Author(s):  
Junichi Mano ◽  
Shuko Hatano ◽  
Yasuaki Nagatomi ◽  
Satoshi Futo ◽  
Reona Takabatake ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sensitive Detection ◽  
Detection Methods ◽  
35S Promoter ◽  
Processed Foods ◽  
Gmo Detection ◽  
Nos Terminator ◽  
Highly Sensitive ◽  
Dna Template

Abstract Current genetically modified organism (GMO) detection methods allow for sensitive detection. However, a further increase in sensitivity will enable more efficient testing for large grain samples and reliable testing for processed foods. In this study, we investigated real-time PCR-based GMO detection methods using a large amount of DNA template. We selected target sequences that are commonly introduced into many kinds of GM crops, i.e., 35S promoter and nopaline synthase (NOS) terminator. This makes the newly developed method applicable to a wide range of GMOs, including some unauthorized ones. The estimated LOD of the new method was 0.005% of GM maize events; to the best of our knowledge, this method is the most sensitive among the GM maize detection methods for which the LOD was evaluated in terms of GMO content. A 10-fold increase in the DNA amount as compared with the amount used under common testing conditions gave an approximately 10-fold reduction in the LOD without PCR inhibition. Our method is applicable to various analytical samples, including processed foods. The use of other primers and fluorescence probes would permit highly sensitive detection of various recombinant DNA sequences besides the 35S promoter and NOS terminator.

Detection of hazelnut (Corylus spp.) in processed foods using real-time PCR

Food Control ◽  
2007 ◽  
Vol 18 (2) ◽  
pp. 140-148 ◽  
Author(s):  
M. Arlorio ◽  
E. Cereti ◽  
J.D. Coïsson ◽  
F. Travaglia ◽  
A. Martelli
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Processed Foods

Real Time PCR to detect hazelnut allergen coding sequences in processed foods

2013 ◽  
Vol 138 (2-3) ◽  
pp. 1976-1981 ◽  
Author(s):  
Elisa Iniesto ◽  
Ana Jiménez ◽  
Nuria Prieto ◽  
Beatriz Cabanillas ◽  
Carmen Burbano ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Processed Foods ◽  
Coding Sequences

scholarly journals Improvements in Methods to Detect Wheat in Heat-processed Foods by Real-time PCR

2020 ◽  
Vol 26 (4) ◽  
pp. 517-526
Author(s):  
Etsuko Miyazaki ◽  
Megumi Kawasaki ◽  
Michihiko Miyamoto ◽  
Keiko Nakamuta ◽  
Takahisa Miyamoto
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Processed Foods

Development of a real-time PCR genotyping assay to identify high oleic acid peanuts (Arachis hypogaea L.)

2009 ◽  
Vol 25 (3) ◽  
pp. 541-548 ◽  
Author(s):  
Noelle A. Barkley ◽  
Kelly D. Chenault Chamberlin ◽  
Ming Li Wang ◽  
Roy N. Pittman
Keyword(s):  
Oleic Acid ◽  
Real Time ◽  
Arachis Hypogaea ◽  
Real Time Pcr ◽  
High Oleic Acid ◽  
High Oleic ◽  
Genotyping Assay ◽  
Arachis Hypogaea L ◽  
Pcr Genotyping
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