scholarly journals Potential of biowastes to remediate diesel fuel contaminated soil

2013 ◽  
Vol 15 (4) ◽  
pp. 474-484 ◽  
Keyword(s):  
Diesel Fuel ◽  
Contaminated Soil ◽  
Oil Pollution ◽  
Animal Health ◽  
Organic Wastes ◽  
Order Kinetic ◽  
Oil Composition ◽  
Biodegradation Rate ◽  
Potato Skin ◽  
Tea Leaf

<p>The unintended release of hydrocarbons into the environment can negatively impact human and animal health, and could further change the characteristics of soils. The aim of the present work was to investigate the rate of biodegradation at 10 and 20% diesel fuel in contaminated soil amended with 10% of three different organic wastes (tea leaf, soy cake, and potato skin) for a period of 126-days. 82 and 25% oil loss was recorded in soil amended with soy cake at 10% and 20 % oil pollution, respectively. Diesel fuel utilizing bacteria counts were high in all organic wastes amended treatments, ranging from 150&times;106 to 176 &times;106 CFU g-1 of soil, compared with the unamended control soil which gave 23 &times;106 CFU g-1. Dehydrogenase activity in soil was markedly enhanced by the application of organic wastes. Diesel oil composition monitored by GC/MS indicated complete degradation of n-C9 &ndash; C12. First-order kinetic model showed that among the three organic wastes used, soy cake had the highest biodegradation rate constant of 0.153 day&minus;1 at 10% oil pollution, while biodegradation rate was 0.033 day&minus;1 at 20% oil pollution. The results showed there is potential for soy cake, potato skin and tea leaf to enhance biodegradation of diesel in contaminated soil.</p>

scholarly journals Biodegradation of Used Motor Oil in Soil Using Organic Waste Amendments

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
O. P. Abioye ◽  
P. Agamuthu ◽  
A. R. Abdul Aziz
Keyword(s):  
Contaminated Soil ◽  
Oil Pollution ◽  
Green Technology ◽  
Lubricating Oil ◽  
Soil Bioremediation ◽  
Order Kinetic ◽  
Human Beings ◽  
Mushroom Compost ◽  
Oil Biodegradation ◽  
Used Lubricating Oil

Soil and surface water contamination by used lubricating oil is a common occurrence in most developing countries. This has been shown to have harmful effects on the environment and human beings at large. Bioremediation can be an alternative green technology for remediation of such hydrocarbon-contaminated soil. Bioremediation of soil contaminated with 5% and 15% (w/w) used lubricating oil and amended with 10% brewery spent grain (BSG), banana skin (BS), and spent mushroom compost (SMC) was studied for a period of 84 days, under laboratory condition. At the end of 84 days, the highest percentage of oil biodegradation (92%) was recorded in soil contaminated with 5% used lubricating oil and amended with BSG, while only 55% of oil biodegradation was recorded in soil contaminated with 15% used lubricating oil and amended with BSG. Results of first-order kinetic model to determine the rate of biodegradation of used lubricating oil revealed that soil amended with BSG recorded the highest rate of oil biodegradation (0.4361 day−1) in 5% oil pollution, while BS amended soil recorded the highest rate of oil biodegradation (0.0556 day−1) in 15% oil pollution. The results of this study demonstrated the potential of BSG as a good substrate for enhanced remediation of hydrocarbon contaminated soil at low pollution concentration.

Monitoring biodegradation of diesel fuel in bioventing processes using in situ respiration rate

2006 ◽  
Vol 53 (4-5) ◽  
pp. 263-272 ◽  
Author(s):  
T.H. Lee ◽  
I.G. Byun ◽  
Y.O. Kim ◽  
I.S. Hwang ◽  
T.J. Park
Keyword(s):  
Respiration Rate ◽  
Diesel Fuel ◽  
Contaminated Soil ◽  
Heterotrophic Bacteria ◽  
Soil Column ◽  
Measuring System ◽  
Co2 Production ◽  
Biodegradation Rate ◽  
On Line

An in situ measuring system of respiration rate was applied for monitoring biodegradation of diesel fuel in a bioventing process for bioremediation of diesel contaminated soil. Two laboratory-scale soil columns were packed with 5 kg of soil that was artificially contaminated by diesel fuel as final TPH (total petroleum hydrocarbon) concentration of 8,000 mg/kg soil. Nutrient was added to make a relative concentration of C:N:P = 100:10:1. One soil column was operated with continuous venting mode, and the other one with intermittent (6 h venting/6 h rest) venting mode. On-line O2 and CO2 gas measuring system was applied to measure O2 utilisation and CO2 production during biodegradation of diesel for 5 months. Biodegradation rate of TPH was calculated from respiration rate measured by the on-line gas measuring system. There were no apparent differences between calculated biodegradation rates from two columns with different venting modes. The variation of biodegradation rates corresponded well with trend of the remaining TPH concentrations comparing other biodegradation indicators, such as C17/pristane and C18/phytane ratio, dehydrogenase activity, and the ratio of hydrocarbon utilising bacteria to total heterotrophic bacteria. These results suggested that the on-line measuring system of respiration rate would be applied to monitoring biodegradation rate and to determine the potential applicability of bioventing process for bioremediation of oil contaminated soil.

scholarly journals Bioremediation of clayey soil contaminated with crude oil: comparison of dynamic and static biopiles in lab-scale

2017 ◽  
Author(s):  
Jorge Antonio Lopes ◽  
Graciane Silva ◽  
Marcia Marques ◽  
Sérgio Machado Correa
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Organic Contaminants ◽  
Clayey Soil ◽  
Total Petroleum Hydrocarbons ◽  
Oil Composition ◽  
Polycyclic Aromatic ◽  
Forced Aeration ◽  
Total Fungi ◽  
Technological Options

Bioremediation of aged and newly clayey soil contaminated with crude oil was investigated in lab-scale using two different strategies (biostimulation-BIOS and bioaugmentation-BIOA), also simulating two different technological options: dynamic biopile (M) and static biopile with forced aeration (B). The inoculum used for bioaugmentation was obtained from the aged contaminated soil. The treatments were performed in triplicates and included one control (original contaminated soil-CONT). The treatments were monitored with soil sampling obtained after 0, 24, 59 and 121 days when the populations of total heterotrophic microorganism (THM), total fungi (TF), and oil-degrading microorganism (ODM) as well as the extracted total petroleum hydrocarbons (TPH) and the 16 polycyclic aromatic hydrocarbons (PAH) prioritized by U.S. EPA were analyzed by gas chromatography. It was observed a trend for reduction of the microbial population density from 0 to 121 days. As expected, the population densities of THM and ODM were much higher in bio-augmented soils in both technologies (BIOA-m and BIOA-b) at day 0. However, after 121 days, the superiority in THM density was observed only in the bioreactor simulating static biopile with forced aeration (BIOA-b). Regarding treatment efficiency, the static biopile with forced aeration performed better in the removal of TPH when associated with bioaugmentation (BIOA-b), being equivalent to the microcosms (simulating dynamic biopile) for the other treatments (CONT and BIOS). For PAH, the superiority of the bioreactor was less conspicuous but observed in both bioremediation strategies (biostimulation BIOS-b and bioaugmentation BIOA-b). The results suggested that regarding TPH, the strategy of bioaugmentation was superior to biostimulation and that the bioreactor (simulating static biopile with forced aeration) reached better contaminant reductions than the microcosm (simulating dynamic biopile). Clayey soil contaminated with crude oil poses big challenges for the bioremediation, due to the texture of the soil favouring adsorption of organic contaminants and due to the complex crude oil composition. The bioprocesses are slow, cleavage of larger molecules are likely to generate smaller hydrocarbons and therefore the elimination of the toxicity is very slow, which may require longer periods and auxiliary tools, such as surfactants.

scholarly journals Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2025
Author(s):  
Xiaoli Dai ◽  
Jing Lv ◽  
Wenxia Wei ◽  
Shaohui Guo
Keyword(s):  
Aromatic Hydrocarbons ◽  
Heavy Oil ◽  
Rate Constants ◽  
Degradation Rate ◽  
Oil Pollution ◽  
Biodegradation Rate ◽  
Saturated Hydrocarbons ◽  
Bacterial Consortia ◽  
Oil Biodegradation ◽  
Biodegradation Efficiency

High-efficiency bioremediation technology for heavy oil pollution has been a popular research topic in recent years. Laccase is very promising for the remediation of heavy oil pollution because it can not only convert bio-refractory hydrocarbons into less toxic or completely harmless compounds, but also accelerate the biodegradation efficiency of heavy oil. However, there are few reports on the use of laccase to enhance the biodegradation of heavy oil. In this study, we investigated the effect of laccase on the bacterial consortia degradation of heavy oil. The degradation efficiencies of bacterial consortia and the laccase-bacterial consortia were 60.6 ± 0.1% and 68.2 ± 0.6%, respectively, and the corresponding heavy oil degradation rate constants were 0.112 day−1 and 0.198 day−1, respectively. The addition of laccase increased the heavy oil biodegradation efficiency (p < 0.05) and biodegradation rate of the bacterial consortia. Moreover, gas chromatography–mass spectrometry analysis showed that the biodegradation efficiencies of the laccase-bacterial consortia for saturated hydrocarbons and aromatic hydrocarbons were 82.5 ± 0.7% and 76.2 ± 0.9%, respectively, which were 16.0 ± 0.3% and 13.0 ± 1.8% higher than those of the bacterial consortia, respectively. In addition, the degradation rate constants of the laccase-bacterial consortia for saturated hydrocarbons and aromatic hydrocarbons were 0.267 day−1 and 0.226 day−1, respectively, which were 1.07 and 1.15 times higher than those of the bacterial consortia, respectively. The degradation of C15 to C35 n-alkanes and 2 to 5-ring polycyclic aromatic hydrocarbons by laccase-bacterial consortia was higher than individual bacterial consortia. It is further seen that the addition of laccase significantly improved the biodegradation of long-chain n-alkanes of C22–C35 (p < 0.05). Overall, this study shows that the combination of laccase and bacterial consortia is an effective remediation technology for heavy oil pollution. Adding laccase can significantly improve the heavy oil biodegradation efficiency and biodegradation rate of the bacterial consortia.

scholarly journals Surfactant-enhanced Bioremediation of n-Hexadecane-contaminated Soil Using Halo-tolerant Bacteria Paenibacillus glucanolyticus sp. Strain T7-AHV Isolated from Marine Environment

2019 ◽  
Vol 33 (1) ◽  
pp. 111-123 ◽  
Author(s):  
Shokouh Ghafari ◽  
Zeynab Baboli ◽  
Sahand Jorfi ◽  
Mehrnoosh Abtahi ◽  
Reza Saeedi ◽  
...  
Keyword(s):  
Marine Environment ◽  
Contaminated Soil ◽  
Tween 80 ◽  
Culture Broth ◽  
Total Petroleum Hydrocarbon ◽  
Operational Parameters ◽  
Biodegradation Rate ◽  
Enhanced Bioremediation ◽  
Water Ratio ◽  
Natural Hydrocarbon

A halo-tolerant bacterial strain Paenibacillus glucanolyticus sp. strain T7-AHV isolated from marine environment was used for bioremediation of n-hexadecane-contaminated soil. Soil/water ratio, initial inoculums volume, surfactant addition, n-hexadecane concentration, and salinity were investigated. The possibility of biosurfactant production by isolated strain was also studied, and the results demonstrated that it was not a biosurfactant producer, based on measurement of the surface tension of culture broth. Both tween 80 and rhamnolipid enhanced the biodegradation of n-hexadecane significantly up to 44 and 46 %, respectively. A biodegradation rate of 39.7 % was observed at salinity level of up to 2 %, and the biodegradation efficiency decreased significantly at higher salinity concentrations. A natural hydrocarbon-contaminated soil sample with total petroleum hydrocarbon (TPH) concentration of 1437 mg kg–1 was subjected to bioremediation using the selected conditions of operational parameters, and a biodegradation rate of 22.1 % was obtained.

scholarly journals Bioremediation of Hydrocarbon Contaminated Soil Using Selected Organic Wastes

2013 ◽  
Vol 18 ◽  
pp. 694-702 ◽  
Author(s):  
P. Agamuthu ◽  
Y.S. Tan ◽  
S.H. Fauziah
Keyword(s):  
Contaminated Soil ◽  
Organic Wastes

The Stress Response and Remediation of Plant on Oil-Contaminated Soil

2010 ◽  
Vol 113-116 ◽  
pp. 1195-1198 ◽  
Author(s):  
Min Qu ◽  
Yan Ming Zhang
Keyword(s):  
Contaminated Soil ◽  
Oil Pollution ◽  
Germination Rate ◽  
Soluble Sugars ◽  
Oil Degradation ◽  
Comprehensive Comparison ◽  
Morphological Differences ◽  
Soil Activity ◽  
Self Protection ◽  
Physiological And Biochemical

Oil pollution is seriously harmful to soil environment and human health. In order to verify the physiological and biochemical response of phytoremediation to oil-contaminated soil and test the relationship between the soil activity and the rate of oil degradation, in the present study, alfalfa, ryegrass, marigold and cosmos are used as the test plants. Through observing the morphological differences, such as germination rate, seedling height and root length, of four plant species under different concentrations of oil-contaminated soil, and measuring the physiological and biochemical indexes, which including MDA, soluble sugars, free proline, soluble protein and chlorophyll etc, and the change of oil degradation rate during the growth process of plant, results proved that plants could improve self-protection capabilities to resist the oil pollution by accumulating osmoregulation substances when they were subject to oil-contaminated stress. By analyzing the changes of polyphenol oxidase, it indicated that the plants can regulate enzyme activity in the oil-contaminated soil, and enhanced it. Comprehensive comparison of the above indexes, we draw a conclusion that alfalfa is an ideal species to repair the oil-contaminated soiland Marigold is the second candidate. In general, our work will lay the theoretic basis for phytoremediation technology research.

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