scholarly journals Anaerobic digestion of olive mill wastewater

2013 ◽  
Vol 11 (3) ◽  
pp. 364-372
Keyword(s):  
Anaerobic Digestion ◽  
White Rot Fungi ◽  
Olive Mill Wastewater ◽  
The Other ◽  
White Rot ◽  
White Rot Fungus ◽  
Stable Operation ◽  
The Stability ◽  
Solids Removal ◽  
Olive Mill

Anaerobic digestion of olive-mill wastewater (OMW) was carried out in a continuously fed mode bioreactor. The bioreactor was operated at different hydraulic retention times (HRTs), using OMW, either raw or pre-treated with white-rot fungi. Two different kinds of feed were tested in this process, one which was thermally treated and subjected to sedimentation, in order to remove the solids contained and the other without any physicochemical treatment (raw OMW). Thermally pretreated OMW did not allow a stable operation even at an HRT of 30d. Further pretreatment of the OMW with a white-rot fungus for removal of the contained phenolics, allowed a stable operation at an HRT of 30 d. On the other hand, simple dilution of the raw wastewater, without any solids removal, lead to a stable operation at an HRT of 30d and was accompanied by higher production of biogas. The presence of the solids in the OMW proved to be a determining factor for the stability of the process and could be attributed to a possible adsorption on the solids of hydrophobic compounds, such as long-chain fatty acids that are toxic to methanogens.

Olive mill wastewater biodegradation potential of white-rot fungi – Mode of action of fungal culture extracts and effects of ligninolytic enzymes

2015 ◽  
Vol 189 ◽  
pp. 121-130 ◽  
Author(s):  
Spyridon Ntougias ◽  
Petr Baldrian ◽  
Constantinos Ehaliotis ◽  
Frantisek Nerud ◽  
Věra Merhautová ◽  
...  
Keyword(s):  
Mode Of Action ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Olive Mill Wastewater ◽  
White Rot ◽  
Fungal Culture ◽  
Olive Mill

scholarly journals Detoxification of Olive Mill Wastewater Using the White Rot Fungus Phanerochaete chrysosporium

2014 ◽  
Vol 3 (1) ◽  
Author(s):  
Mazen Salman ◽  
Nawaf Abu-Khalaf ◽  
Basima Abu Rumaileh ◽  
Mohamad Jawabreh ◽  
Ruba Abuamsha
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Olive Mill Wastewater ◽  
White Rot ◽  
White Rot Fungus ◽  
Olive Mill

Potential role of Bacillus endospores in soil amended by olive mill wastewater

2010 ◽  
Vol 61 (11) ◽  
pp. 2873-2879 ◽  
Author(s):  
Gino Naclerio ◽  
Antonio Falasca ◽  
Emma Petrella ◽  
Valentina Nerone ◽  
Federica Cocco ◽  
...  
Keyword(s):  
Potential Role ◽  
Laccase Activity ◽  
White Rot Fungi ◽  
Olive Mill Wastewater ◽  
White Rot ◽  
Bacillus Species ◽  
Italian Law ◽  
Bacillus Spores ◽  
Olive Mill

The main aim of this work was to know how spread is laccase activity in spores of Bacillus species isolated from a soil where Italian law allows olive mill wastewater (OMW) spreading, and to investigate the potential role of such autochthonous soil microorganisms in degradation of OMW phenols, and prevention of groundwater pollution. Laccase activity was detected for the first time in spores of wild-type Bacilluspumilus, B. cereus sensu lato, and B. amyloliquefaciens strains. Because B. pumilus, B. cereus sensu lato, and B. amyloliquefaciens, together with B. subtilis account for a total of 93% of Bacillus isolates at the study site, the nearly totality of Bacillus spores reveals laccase activity. Thus, taking also into consideration that Bacillus spores are more abundant (about 100-fold) than white-rot fungi (that possess a well known extracellular, radical-based ligninolytic enzyme system capable of degrading OMW phenols) in the studied soil, these spores may contribute to in-situ degradation of OMW phenols. This role is further emphasized by dilution of crude OMW during infiltration of rainwater through soil that allows to minimize the antibacterial activity of phenols. The widespread presence of Bacillus spores in soils indicates a potential detoxifying role of these spores in a broader context.

The Onset of Lignin-Modifying Enzymes, Decrease of AOX and Color Removal by White-Rot Fungi Grown on Bleach Plant Effluents

1991 ◽  
Vol 24 (3-4) ◽  
pp. 189-198 ◽  
Author(s):  
V. P. Lankinen ◽  
M. M. Inkeröinen ◽  
J. Pellinen ◽  
A. I. Hatakka
Keyword(s):  
Lignin Peroxidase ◽  
Active Form ◽  
White Rot Fungi ◽  
Pulp Mill ◽  
Color Removal ◽  
Effluent Treatment ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Peroxidases ◽  
Pulp Mill Effluents

Decrease of adsorbable organic chlorine (AOX) is becoming the most important criterion for the efficiency of pulp mill effluent treatment in the 1990s. Two methods, designated MYCOR and MYCOPOR which utilize the white-rot fungus Phanerochaete chrysosporium have earlier been developed for the color removal of pulp mill effluents, but the processes have also a capacity to decrease the amount of chlorinated organic compounds. Lignin peroxidases (ligninases) produced by P. chrvsosporium may dechlorinate chlorinated phenols. In this work possibilities to use selected white-rot fungi in the treatment of E1-stage bleach plant effluent were studied. Phlebia radiata. Phanerochaete chrvsosporium and Merulius (Phlebia) tremellosus were compared in shake flasks for their ability to produce laccase, lignin peroxidase(s) and manganese-dependent peroxidase(s) and to remove color from a medium containing effluent. Softwood bleaching effluents were treated by carrier-immobilized P. radiata in 2 1 bioreactors and a 10 1 BiostatR -fermentor. Dechlorination was followed using Cl ion and AOX determinations. All fungi removed the color of the effluent. In P. radiata cultivations AOX decrease was ca. 4 mg l−1 in one day. Apparent lignin peroxidase activities as determined by veratryl alcohol oxidation method were negligible or zero in a medium with AOX content of ca. 60 mg l−1, prepared using about 20 % (v/v) of softwood effluent. However, the purification of extracellular enzymes implied that large amounts of lignin peroxidases were present in the medium and, after the purification, in active form. Enzyme proteins were separated using anion exchange chromatography, and they were further characterized by electrophoresis (SDS-PAGE) to reveal the kind of enzymes that were present during AOX decrease and color removal. The most characteristic lignin peroxidase isoenzymes in effluent media were LiP2 and LiP3.

scholarly journals Olive mill wastewater effects on durum wheat crop attributes and soil microbial activities: A pilot study in Syria

2021 ◽  
Author(s):  
Jehan Khalil ◽  
Hasan Habib ◽  
Michael Alabboud ◽  
Safwan Mohammed
Keyword(s):  
Durum Wheat ◽  
Germination Rate ◽  
Soil Samples ◽  
Olive Mill Wastewater ◽  
The Other ◽  
Wheat Crop ◽  
Microbial Activities ◽  
Soil Microbial ◽  
Growing Seasons ◽  
Olive Mill

AbstractOlive mill wastewater is one of the environmental problems in semiarid regions. The main goals of this study were to investigate the impacts of different olive mill wastewater levels on durum wheat (Triticum aestivum var. Douma1) production and soil microbial activities (i.e., bacteria and fungi). A pot experiment was conducted during the growing seasons 2015/2017 to evaluate the effect of three levels of olive mill wastewater on both growth and productivity attributes of wheat. Vertisol soil samples were collected from southern Syria. Two months before wheat cultivation, three levels of olive mill wastewater: T5 (5 L/m 2), T10 (10 L/m2) and T15 (15 L/m 2) were added to pots filled with the collected soil samples. Also, a control (T0) free of olive mill wastewater was considered as a reference. Results showed a significant increase (p < 0.05) in germination rate (%), plant height (cm), ear length (cm), kernels number, kernels weight per ear (g) and grain yield (g/m2) compared to control. However, T5 treatment did not induce a significant increase in terms of ear length, kernels weight per ear or yield (in the second season). On the other hand, T10 treatment had recorded the best results compared with the other two treatments (T5, T15). Similarly, the results showed a significant increase in the number of bacterial and fungi cells by increasing olive mill wastewater concentration. This research provides promising results toward using olive mill wastewater in an eco-friendly way under Syrian conditions.

Enhancement of the anaerobic digestion of olive mill wastewater by the removal of phenolic inhibitors

1992 ◽  
Vol 27 (4) ◽  
pp. 231-237 ◽  
Author(s):  
R. Borja ◽  
A. Martin ◽  
R. Maestro ◽  
J. Alba ◽  
J.A. Fiestas
Keyword(s):  
Anaerobic Digestion ◽  
Olive Mill Wastewater ◽  
Olive Mill
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