scholarly journals Penentuan β-karoten dalam buah wortel (Daucus Carota) secara kromatografi cair kinerja tinggi (U-HPLC)

2019 ◽  
Vol 4 (1) ◽  
pp. 36
Author(s):  
Sonlimar Mangunsong ◽  
Rifqi Assiddiqy ◽  
Eka Puspa Sari ◽  
Priscila Natalia Marpaung ◽  
Rahma Arum Sari
Keyword(s):  
Free Radicals ◽  
Daucus Carota ◽  
High Performance ◽  
Calcium Salt ◽  
Beta Carotene ◽  
The Body ◽  
Organic Chemical ◽  
Chloroform Methanol ◽  
Β Carotene

The lifestyle of people who tend to be unhealthy causes a lot of free radicals in the body which can cause various diseases, especially degenerative diseases. To protect the body from attacks by free radicals, the body needs antioxidants such as β-carotene. One of the vegetables containing β-carotene is “wortel” Daucus carota, that  is very large in agriculture. The spread of beta carotene from carrots with chemical solvents has been done a lot, but without chemical solvents it has never been done. Based on the color, the content of β-carotene contained in carrots determines the content of beta carotene. The juice obtained by calcium salt was then centrifuged for 3000 rpm 15 minutes. The pellets are separated from the solution, evaporated to dryness, measured at a wavelength of 450-460. Pellets as beta-carotene calcium salts were analyzed by U-HPLC. The next pellet is measured at 460 wavelengths. The levels are determined by high performance liquid chromatography using C18 column and the mobile phase of chloroform-methanol (95:5) with a flow rate of 1 ml / minute at a wavelength of 460 nm. The content of β-carotene in the examination is up to 92,5%. The retention time obtained is 1,903 minutes. The results showed that this method can be used for the withdrawal and determination of β-carotene levels without organic chemical solvents.Pola  hidup masyarakat yang cenderung tidak sehat menyebabkan  banyak radikal bebas di dalam tubuh yang dapat mengakibatkan berbagai penyakit terutama penyakit degeneratif. Perlindungan tubuh dari serangan radikal bebas, perlu antioksidan seperti β-karoten. Salah satu sayuran  yang mengandung β-karoten adalah wortel, jumlahnya sangat banyak dalam pertanian. Berdasarkan warnanya, maka kandungan β-karoten yang terdapat dalam wortel menjadi penentu  kandungan beta karoten Metode ektraksi yang aman dari bahan pelarut kimia mutlak diperlukan.  Untuk mengetahui kandungan β-karoten dalam wortel,  lebih  dahulu dilakukan penghalusan  dengan blender, kemudian sari difilter /dipisahkan dari ampasnya. Sari  yangdiperleh ditambahkan garam kalsium kemudian disentrifus 3000 rpm  15 menit. Pelet dipisahkan dari larutannya, diuapkan hingga kering, Diukur pada panjang gelombang 450-460. Pelet sebagai garam kalsium betakaroten dianalisa dengan U-HPLC.  Pelet selanjutkan diukur pada panjang gelombang 460. ditetapkan kadarnya secara kromatografi cair kinerja tinggi menggunakan kolom C18 dan fase gerak kloroform–metanol (95:5) dengan laju alir 1 ml/menit pada panjang gelombang 460 nm. Kandungan β-karoten dalam pemeriksaan  sampai 92,5%. Waktu retensi yang diperoleh adalah 1,903 menit.  Hasil penelitian menunjukkan bahwa metode ini dapat digunakan untuk penarikan dan penetapan kadar β-karoten dalam   tanpa pelarut kimia organik.

Two Validated Chromatographic Methods for Determination of Ciprofloxacin HCl, One of its Specified Impurities and Fluocinolone Acetonide in Newly Approved Otic Solution

2021 ◽  
Author(s):  
Mahmoud A Tantawy ◽  
Israa A Wahba ◽  
Samah S Saad ◽  
Nesrin K Ramadan
Keyword(s):  
High Performance ◽  
Fluocinolone Acetonide ◽  
Ultraviolet Detection ◽  
Chromatographic Methods ◽  
Bulk Powder ◽  
Developing System ◽  
Layer Chromatography ◽  
Chloroform Methanol ◽  
Linear Regressions

Abstract Two sensitive, selective and precise chromatographic methods have been established for concomitant quantification of ciprofloxacin HCl (CIP), fluocinolone acetonide (FLU) along with ciprofloxacin impurity A (CIP-imp A). The first method was thin-layer chromatography (TLC-densitometry) where separation was accomplished using TLC silica plates 60 G.F254 as a stationary phase and chloroform–methanol–33%ammonia (4.6:4.4:1, by volume) as a developing system. The obtained plates were scanned at 260 nm over concentration ranges of 1.0–40.0, 0.6–20.0 and 1.0–40.0 μg band−1 for CIP, FLU and CIP-imp A, respectively. The second method was based on high-performance liquid chromatography using a Zorbax ODS column (5 μm, 150 × 4.6 mm i.d.) where adequate separation was achieved through a mobile phase composed of phosphate buffer pH 3.6–acetonitrile (45:55, v/v) at flow rate 1.0 mL min−1 with ultraviolet detection at 254 nm. Linear regressions were obtained in the range of 1.0–40.0 μg mL−1 for CIP, 0.6–20.0 μg mL−1 for FLU and 1.0–40.0 μg mL−1 for CIP-imp A. The suggested methods were validated in compliance with the International Conference on Harmonization guidelines and were successfully applied for determination of CIP and FLU in bulk powder and newly marketed otic solution.

scholarly journals Determination of gangliosides as 2,4-dinitrophenylhydrazides by high-performance liquid chromatography

1986 ◽  
Vol 235 (3) ◽  
pp. 755-761 ◽  
Author(s):  
K Miyazaki ◽  
N Okamura ◽  
Y Kishimoto ◽  
Y C Lee
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Normal Phase ◽  
Water Soluble ◽  
Acid System ◽  
Total Lipids ◽  
Acetylneuraminic Acid ◽  
Chloroform Methanol ◽  
Phase Column

A specific, sensitive and easily performed method for the determination of gangliosides in tissue was developed. After removal of water-soluble compounds, total lipids were extracted from tissue and then treated with 2,4-dinitrophenylhydrazine hydrochloride and dicyclohexylcarbodi-imide in dimethylformamide at 0 degrees C to form ganglioside hydrazides. After removal of excess reagents by column chromatography on silicic acid, the ganglioside 2,4-dinitrophenylhydrazides were eluted from the column and analysed by h.p.l.c. with the use of a silica-gel normal-phase column eluted with an isocratic chloroform/methanol/water/acetic acid system. The addition of CaCl2 improved the separation of GM3 ganglioside containing N-acetylneuraminic acid from that containing N-glycollylneuraminic acid. 2,4-Dinitrophenylhydrazide peaks were measured by the absorbance at 342 nm. Quantification of GM3, GM2, GM1, GD1a, GD1b, GT1b and LM1 gangliosides was linear in a range 0.02-1.6 nmol. GM4, GD3, GT1a and GQ1b gangliosides also yielded distinct peaks, although the range of linearity was not examined. This method was applied to the analysis of the total lipids of rat brain and hepatocytes.

scholarly journals Analysis of βeta-carotene from Jongi (Dillenia Serrata Thunb.) as a source of vitamin A

2021 ◽  
Vol 10 (2) ◽  
pp. 184-190
Author(s):  
Khairun Nisa ◽  
Ryka Marina Walanda
Keyword(s):  
Chronic Disease ◽  
Vitamin A ◽  
Essential Role ◽  
Antioxidant Activities ◽  
Beta Carotene ◽  
Neurological Function ◽  
The Body ◽  
Yellow Orange ◽  
Β Carotene ◽  
Healthy Eyes

Beta-carotene is converted in the body to vitamin A, a powerful antioxidant that plays an essential role in maintaining healthy eyes, skin, and neurological function. Beta-carotene and other carotenoids have antioxidant activities and are valued for their ability to prevent chronic disease. β-carotene is found in yellow, orange, red, and green fruits. The purpose of this study was to determine the β-carotene in Jongi. Jongi (Dillenia Serrata Thunb.) is an endemic fruit of Sulawesi which contains β-carotene. The samples used in this study were raw jongi and ripe jongi extracted with n-hexane: acetone (1:4). To the samples were analyzed quantitatively using UV-Vis spectrophotometry at a wavelength of 450.00 nm. The results showed that the β-carotene in raw jongi was 0.3554 mg/100 g and the β- carotene in ripe jongi was 1.1841 mg/100 g. Ripe jongi consist of more β-carotene than the raw jongi, hence better as a source of Vitamin A.

Simultaneous determination of vitamins A and E and carotenoids in plasma by reversed-phase HPLC in elderly and younger subjects

1993 ◽  
Vol 39 (11) ◽  
pp. 2229-2234 ◽  
Author(s):  
Z Zaman ◽  
P Fielden ◽  
P G Frost
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Ammonium Acetate ◽  
Beta Carotene ◽  
High Performance Liquid Chromatographic ◽  
Solvent Mixture ◽  
Reversed Phase ◽  
Alpha Tocopherol ◽  
Elderly Subjects

Abstract A reversed-phase high-performance liquid-chromatographic method for the simultaneous determination of retinol, alpha-tocopherol, alpha-carotene, beta-carotene, cryptoxanthin, lutein/zeaxanthin, and lycopene is described. This method was applied to plasma measurements in healthy young and elderly subjects. The plasma, deproteinized with ethanol, is extracted twice with n-hexane. After evaporation, the residue is dissolved in 50 microL of tetrahydrofuran and made up to 200 microL with ethanol. Samples (50 microL) are injected onto a 250 x 4.6 mm column of 5-microns-particle Spherisorb ODS1 (Phase Separations) that had been equilibrated with solvent mixture A:B (90:10 by vol) [A = 100 mmol/L ammonium acetate in methanol: acetonitrile (80:20 by vol) and B = 100 mmol/L ammonium acetate in water] at 2 mL/min. The analytes are eluted by running a 12-min linear gradient to 100% A; solvent A is then maintained for 10 min. Intrabatch CVs were 2.3%, 3.3%, 2.8%, 3.6%, 3.6%, and 3.0% for retinol, alpha-tocopherol, lutein/zeaxanthin, cryptoxanthin, lycopene, and beta-carotene, respectively. The corresponding interbatch CVs were 4.9%, 5.8%, 12.3%, 6.5%, 8.0%, and 3.4%.

scholarly journals Analysis of Vitamin A in Multivitamin Products

2021 ◽  
Vol 11 (3) ◽  
pp. 174-184
Author(s):  
A. S. Alekseeva ◽  
T. B. Shemeryankina ◽  
M. N. Lyakina ◽  
M. S. Smirnova ◽  
E. P. Fedorova ◽  
...  
Keyword(s):  
Vitamin A ◽  
High Performance ◽  
Beta Carotene ◽  
Test Methods ◽  
Retinyl Palmitate ◽  
Test Method ◽  
Medicinal Products ◽  
Retinyl Acetate ◽  
Preliminary Purification

Vitamin A is present in multivitamin products mainly in the form of retinol esters: retinyl acetate, retinyl palmitate, and beta carotene—retinol precursor (dimer) found in plants, which is capable of converting into retinol in liver cells. Retinol is determined in medicinal products primarily by high performance liquid chromatography (HPLC), with preliminary purification and vitamin isolation by liquid-liquid extraction. However, scientific literature also describes other methods of sample preparation and analysis of such compounds. An important issue is differentiation of vitamin A from other fat-soluble vitamins often included as components in multivitamin products. The aim of the study was to analyse and summarise data on current methods used for determination of vitamin A and its derivatives in medicinal products. The authors analysed the range of vitamin A products authorised in the Russian Federation, and the test methods described in their product specification files. The study demonstrated that the test method most often used for determination of retinol esters was HPLC with isocratic elution mode using octadecylsilyl packing in the reverse-phase mode, and, less frequently, aminopropylsilyl packing in the normal phase mode. Determination of beta carotene in medicinal products is most often performed using spectrophotometry. 

scholarly journals Determination of optimum extraction condition for edible oil and β-carotene from carrot peel (Daucus carota L.) using supercritical carbon dioxide

2019 ◽  
Vol 18 (2) ◽  
pp. 97-115
Author(s):  
Suthida Akkarachaneeyakorn ◽  
Tanasith Tangpaiboonpongsa ◽  
Mintra Chuakam ◽  
Kwanchanok Dasiri ◽  
Pattama Phasuthan ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Daucus Carota ◽  
Edible Oil ◽  
Extraction Condition ◽  
Daucus Carota L ◽  
Optimum Extraction ◽  
Β Carotene ◽  
Optimum Extraction Condition
Sign in / Sign up

Export Citation Format

Share Document