Role of phyllosphere microbes in Ice Nucleation Activity and Its impact on drought mitigation of tomato

2019 ◽  
Vol 106 (Spl) ◽  
Author(s):  
Guna M ◽  
Panneerselvam S ◽  
Subramanian K.S ◽  
Krishnamoorthy A.S. ◽  
Lakshmanan, A.
Keyword(s):  
Ice Nucleation ◽  
Drought Mitigation ◽  
Nucleation Activity ◽  
Ice Nucleation Activity

scholarly journals Role of Ice Nucleation and Antifreeze Activities in Pathogenesis and Growth of Snow Molds

2000 ◽  
Vol 90 (4) ◽  
pp. 354-361 ◽  
Author(s):  
Christopher S. Snider ◽  
Tom Hsiang ◽  
Guiying Zhao ◽  
Marilyn Griffith
Keyword(s):  
Low Temperature ◽  
Host Plants ◽  
Ice Nucleation ◽  
Microdochium Nivale ◽  
Ice Formation ◽  
Subzero Temperatures ◽  
Nucleation Activity ◽  
Ice Nucleation Activity ◽  
Antifreeze Activity

We examined the ability of snow molds to grow at temperatures from -5 to 30°C and to influence the growth of ice through assays for ice nucleation and antifreeze activities. Isolates of Coprinus psychromorbidus (low temperature basidiomycete variant), Microdochium nivale, Typhula phacorrhiza, T. ishikariensis, T. incarnata, and T. canadensis all grew at -5°C, whereas Sclerotinia borealis and S. homoeocarpa did not grow at temperatures below 4°C. The highest threshold ice nucleation temperature was -7°C. Because snow molds are most damaging to their hosts at temperatures above this, our results imply that the pathogenesis of these fungi is not dependent on ice nucleation activity to cause freeze-wounding of host plants. All snow molds that grew at subzero temperatures also exhibited antifreeze activity in the growth medium and in the soluble and insoluble hyphal fractions, with the exception of M. nivale and one isolate of T. canadensis. The lack of high ice nucleation activity combined with the presence of antifreeze activity in all fungal fractions indicates that snow molds can moderate their environment to inhibit or modify intra- and extracellular ice formation, which helps explain their ability to grow at subzero temperatures under snow cover.

scholarly journals Seasonal ice nucleation activity of water samples from alpine rivers and lakes in Obergurgl, Austria

2021 ◽  
pp. 149442
Author(s):  
Philipp Baloh ◽  
Regina Hanlon ◽  
Christopher Anderson ◽  
Eoin Dolan ◽  
Gernot Pacholik ◽  
...  
Keyword(s):  
Water Samples ◽  
Ice Nucleation ◽  
Rivers And Lakes ◽  
Nucleation Activity ◽  
Alpine Rivers ◽  
Ice Nucleation Activity

Ice nucleation activity of iron oxides via immersion freezing and an examination of the high ice nucleation activity of FeO

2021 ◽  
Vol 23 (5) ◽  
pp. 3565-3573
Author(s):  
Esther Chong ◽  
Katherine E. Marak ◽  
Yang Li ◽  
Miriam Arak Freedman
Keyword(s):  
Iron Oxides ◽  
Functional Groups ◽  
Ice Nucleation ◽  
Mechanical Processing ◽  
Immersion Freezing ◽  
Nucleation Activity ◽  
Ice Nucleation Activity

FeO has enhanced ice nucleation activity due to functional groups that are exposed upon mechanical processing.

scholarly journals Effects of atmospheric conditions on ice nucleation activity of <i>Pseudomonas</i>

2012 ◽  
Vol 12 (22) ◽  
pp. 10667-10677 ◽  
Author(s):  
E. Attard ◽  
H. Yang ◽  
A.-M. Delort ◽  
P. Amato ◽  
U. Pöschl ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Pseudomonas Syringae ◽  
Ice Nucleation ◽  
Acidic Ph ◽  
Bacterial Cells ◽  
Atmospheric Conditions ◽  
Ice Nucleation Protein ◽  
Ice Melt ◽  
Nucleation Activity ◽  
Ice Nucleation Activity

Abstract. Although ice nuclei from bacterial origin are known to be efficient at the highest temperatures known for ice catalysts, quantitative data are still needed to assess their role in cloud processes. Here we studied the effects of three typical cloud conditions (i) acidic pH (ii) NO2 and O3 exposure and (iii) UV-A exposure on the ice nucleation activity (INA) of four Pseudomonas strains. Three of the Pseudomonas syringae strains were isolated from cloud water and the phyllosphere and Pseudomonas fluorescens strain CGina-01 was isolated from Antarctic glacier ice melt. Among the three conditions tested, acidic pH caused the most significant effects on INA likely due to denaturation of the ice nucleation protein complex. Exposure to NO2 and O3 gases had no significant or only weak effects on the INA of two P. syringae strains whereas the INA of P. fluorescens CGina-01 was significantly affected. The INA of the third P. syringae strain showed variable responses to NO2 and O3 exposure. These differences in the INA of different Pseudomonas suggest that the response to atmospheric conditions could be strain-specific. After UV-A exposure, a substantial loss of viability of all four strains was observed whereas their INA decreased only slightly. This corroborates the notion that under certain conditions dead bacterial cells can maintain their INA. Overall, the negative effects of the three environmental factors on INA were more significant at the warmer temperatures. Our results suggest that in clouds where temperatures are near 0 °C, the importance of bacterial ice nucleation in precipitation processes could be reduced by some environmental factors.

scholarly journals Ice nucleation activity identified in some phytopathogenic Fusarium species

2005 ◽  
Vol 77 (2) ◽  
pp. 83-92 ◽  
Author(s):  
C. Richard ◽  
J.-G. Martin ◽  
S. Pouleur
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Species ◽  
Freezing Temperature ◽  
Ice Nucleation ◽  
Free Living ◽  
Factors Affecting ◽  
Pure Cultures ◽  
Nucleation Activity ◽  
Ice Nucleation Activity ◽  
Positive Controls

In order to know which species of Fusarium are ice nucleating and to determine the factors affecting their pathogenicity, ice nucleation activity (INA) was examined in Fusarium oxysporum, F. sporotrichioides, and F. tricinctum. Positive controls (lna+) used were F. acuminatum and F. avenaceum. The test for fungal INA was done with a simple and rapid tube nucleation assay. Twelve out of the 42 F. oxysporum isolates, and 8 out of 14 F. tricinctum isolates were lna+. No INA was detected in F sporotrichioides. In this test the threshold freezing temperature tended to increase with culture age, reaching a peak of -1°C in a few samples, which is as high as the warmest INA reported for bacteria, and higher than the INA detected in pure cultures of free-living fungi, lichen fungi, lichen algae and cyanobacteria. This is the first report of INA for F oxysporum.

scholarly journals High-Flow-Rate Impinger for the Study of Concentration, Viability, Metabolic Activity, and Ice-Nucleation Activity of Airborne Bacteria

2017 ◽  
Vol 51 (19) ◽  
pp. 11224-11234 ◽  
Author(s):  
Tina Šantl-Temkiv ◽  
Pierre Amato ◽  
Ulrich Gosewinkel ◽  
Runar Thyrhaug ◽  
Anaïs Charton ◽  
...  
Keyword(s):  
Flow Rate ◽  
Metabolic Activity ◽  
Ice Nucleation ◽  
High Flow ◽  
High Flow Rate ◽  
Airborne Bacteria ◽  
Nucleation Activity ◽  
Ice Nucleation Activity

Ice nucleation activity of bacteria isolated from snow compared with organic and inorganic substrates

2008 ◽  
Vol 5 (6) ◽  
pp. 373 ◽  
Author(s):  
Roya Mortazavi ◽  
Christopher T. Hayes ◽  
Parisa A. Ariya
Keyword(s):  
Bacterial Species ◽  
Ice Nucleation ◽  
Detailed Knowledge ◽  
Bacterial Populations ◽  
Inorganic Aerosols ◽  
Ice Nucleators ◽  
Species Population ◽  
Nucleation Activity ◽  
Ice Nucleation Activity ◽  
Snow Samples

Environmental context. Biological ice nucleators have been found to freeze water at very warm temperatures. The potential of bio-aerosols to greatly influence cloud chemistry and microphysics is becoming increasingly apparent, yet detailed knowledge of their actual role in atmospheric processes is lacking. The formation of ice in the atmosphere has significant local, regional and global influence, ranging from precipitation to cloud nucleation and thus climate. Ice nucleation tests on bacteria isolated from snow and laboratory-grown bacteria, in comparison with those of known organic and inorganic aerosols, shed light on this issue. Abstract. Ice nucleation experiments on bacteria isolated from snow as well as grown in the laboratory, in comparison with those of known organic and inorganic aerosols, examined the importance of bio-aerosols on cloud processes. Snow samples were collected from urban and suburban sites in the greater Montreal region in Canada (45°28′N, 73°45′W). Among many snow bacterial isolates, eight types of bacterial species, none belonging to known effective ice nucleators such as Pseudomonas or Erwinia genera, were identified to show an intermediate range of ice nucleation activity (–12.9 ± 1.3°C to –17.5 ± 2.8°C). Comparable results were also obtained for molten snow samples and inorganic suspensions (kaolin and montmorillonite) of buffered water solutions. The presence of organic molecules (oxalic, malonic and succinic acids) had minimal effect (<2°C) on ice nucleation. Considering experimental limitations, and drawing from observation in snow samples of a variety of bacterial populations with variable ice-nucleation ability, a shift in airborne-species population may significantly alter glaciation processes in clouds.

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