scholarly journals Effect of Different Levels of Symbiotic on the Japanese Quail Performance, Immune Response and Small Intestine Histomorphology

2019 ◽  
Vol 10 (26) ◽  
pp. 1-9
Author(s):  
Seyede sima mohammadi ◽  
◽  
hamidreza khodaei ◽  
sohail mir habibi ◽  
hossein manafi rasi ◽  
...  
Keyword(s):  
Immune Response ◽  
Small Intestine ◽  
Japanese Quail ◽  
Different Levels

The effect of oxyclozanide on Hymenolepis microstoma and H. diminuta

Parasitology ◽  
1973 ◽  
Vol 66 (2) ◽  
pp. 355-365 ◽  
Author(s):  
C. A. Hopkins ◽  
P. M. Grant ◽  
Helen Stallard
Keyword(s):  
Immune Response ◽  
Small Intestine ◽  
Single Dose ◽  
Bile Duct ◽  
Dose Level ◽  
Harmful Effect ◽  
Maximum Level ◽  
Adult Size ◽  
Hymenolepis Microstoma

The effect of oxyclozanide (2,2′-dihydroxy-3,3′,5,5′,6-pentachlorobenzanilide) on Hymenolepis microstoma in the bile duct of mice, and H. diminuta in the small intestine of mice and rats was measured. Oxyclozanide at doses as low as 4mg/kg removed 13-day-old H. diminuta and caused no obvious harmful effect to the rat host up to the maximum level (256 mg/kg) tested. Worms were displaced and degenerating within 1 h. Results in mice were more difficult to assess because of the immune response, but similar total amounts of oxyclozanide caused destrobilation and loss of 7-day-old H. diminuta. Oxyclozanide was less effective against 21-day-old H. microstoma attached in the bile duct. Approximately half the strobila was lost following dosing at 5 mg/kg and progressively greater amounts as the dose level was increased. At 50 mg/kg worm loss commenced but even at 150 mg/kg 25 % of worms survived. The time taken to regrow to the original adult size varied but was complete within 7–9 days at levels up to 25 mg/kg. Double dosing at 5-day intervals did not enhance the effect of a single dose. The apparent existence of a sensitivity gradient down the strobila in H. microstoma is discussed.

scholarly journals MEAN SURFACE TEMPERATURE OF JAPANESE QUAIL EXPOSED TO DIFFERENT LEVELS OF AIR VELOCITY AND TEMPERATURE AT START OF LAY

2020 ◽  
Vol 4 (2) ◽  
pp. 15-18
Author(s):  
Tatiany Carvalho dos Santos ◽  
Richard Stephen Gates ◽  
Ilda De Fátima Ferreira Tinôco ◽  
Sérgio Zolnier ◽  
Letícia Cibele da Silva Ramos Freitas
Keyword(s):  
Surface Temperature ◽  
Air Temperature ◽  
Japanese Quail ◽  
Systems Engineering ◽  
Air Velocity ◽  
Agricultural Engineering ◽  
Initial Stage ◽  
Different Temperatures ◽  
The Mean ◽  
Different Levels

The objective of this study was to evaluate the effect of different air velocities and temperature at the feeder on mean surface temperature of Japanese quail during the initial stage of laying. The experiment was carried out at the Center for Research in Environment and Agroindustry Systems Engineering (AMBIAGRO), Department of Agricultural Engineering, Federal University of Viçosa, Viçosa/MG, Brazil. A total of 216 Japanese quail in the initial laying phase were placed in four environmental chambers with different temperatures and air velocity, where they were housed and distributed randomly in 2 galvanized wire cages, with 3 partitions each and 27 birds/cage, and a density of approximately 155.6 cm²/bird. The experimental design consisted of randomized blocks with replications of two treatments (air velocity at the feeder: 0, 1, 2, and 3 m/s and air temperature: 17, 23, 29 and 35°C). The mean surface temperature was analyzed by Two-Way ANOVA, with treatment means separated by the Tukey test (P < 0.05). There was a significant positive correlation between air temperature and mean surface temperature (MST). Air velocity is important in removing heat from the surface of birds.

scholarly journals 2,2’4,4’-Tetrabromodiphenyl Ether (PBDE-47) Modulates the Intracellular miRNA Profile, sEV Biogenesis and Their miRNA Cargo Exacerbating the LPS-Induced Pro-Inflammatory Response in THP-1 Macrophages

2021 ◽  
Vol 12 ◽  
Author(s):  
Valeria Longo ◽  
Alessandra Longo ◽  
Giorgia Adamo ◽  
Antonino Fiannaca ◽  
Sabrina Picciotto ◽  
...  
Keyword(s):  
Immune Response ◽  
Inflammatory Response ◽  
Innate Immune Response ◽  
De Novo ◽  
In Silico Analysis ◽  
Innate Immune ◽  
Macrophage Cell ◽  
Intracellular Expression ◽  
Different Levels ◽  
Silico Analysis

The 2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) is one of the most prominent PBDE congeners detected in the environment and in animal and human tissues. Animal model experiments suggested the occurrence of PBDE-induced immunotoxicity leading to different outcomes and recently we demonstrated that this substance can impair macrophage and basophil activities. In this manuscript, we decided to further examine the effects induced by PBDE-47 treatment on innate immune response by looking at the intracellular expression profile of miRNAs as well as the biogenesis, cargo content and activity of human M(LPS) macrophage cell-derived small extracellular vesicles (sEVs). Microarray and in silico analysis demonstrated that PBDE-47 can induce some epigenetic effects in M(LPS) THP-1 cells modulating the expression of a set of intracellular miRNAs involved in biological pathways regulating the expression of estrogen-mediated signaling and immune responses with particular reference to M1/M2 differentiation. In addition to the cell-intrinsic modulation of intracellular miRNAs, we demonstrated that PBDE-47 could also interfere with the biogenesis of sEVs increasing their number and selecting a de novo population of sEVs. Moreover, PBDE-47 induced the overload of specific immune related miRNAs in PBDE-47 derived sEVs. Finally, culture experiments with naïve M(LPS) macrophages demonstrated that purified PBDE-47 derived sEVs can modulate macrophage immune response exacerbating the LPS-induced pro-inflammatory response inducing the overexpression of the IL-6 and the MMP9 genes. Data from this study demonstrated that PBDE-47 can perturb the innate immune response at different levels modulating the intracellular expression of miRNAs but also interfering with the biogenesis, cargo content and functional activity of M(LPS) macrophage cell-derived sEVs.

Comparison of gastrointestinal responses to CCK-8 and associated with vomiting

1988 ◽  
Vol 254 (2) ◽  
pp. G254-G263 ◽  
Author(s):  
I. M. Lang ◽  
J. Marvig ◽  
S. K. Sarna
Keyword(s):  
Small Intestine ◽  
Motor Responses ◽  
Cholecystokinin Octapeptide ◽  
Bipolar Electrodes ◽  
Chronic Recording ◽  
Force Transducers ◽  
Fasted State ◽  
Peripheral Mechanism ◽  
Gastrointestinal Motor ◽  
Different Levels

The gastrointestinal motor and myoelectric responses associated with vomiting induced by apomorphine (APO) and activated by cholecystokinin octapeptide (CCK-8) were compared as well as the mechanisms of initiation of these responses. Twelve dogs were surgically implanted with strain-gauge force transducers or bipolar electrodes for chronic recording of contractile or electrical activity. The responses to CCK-8 were determined in the fasted state and compared with the gastrointestinal motor and myoelectric correlates of vomiting activated by APO. After recording control responses, the effects of the following agents on these responses were determined: atropine, domperidone, and proglumide. In addition, the effects of supradiaphragmatic vagotomy or splanchnicectomy were determined. We found that CCK-8 activated contractile and myoelectric responses in the absence of vomiting, which were similar in most respects to those found in association with vomiting. These responses included 1) the retrograde giant contraction (RGC) and 2) the post-RGC phasic contractions. These RGCs were similar with respect to their activation in an all-or-none fashion, magnitude, duration, and position in the small intestine. The myoelectric correlates of these motor responses were similar qualitatively and quantitatively. The responses activated by APO and CCK-8 differed with respect to their coordination at different levels of the gastrointestinal tract. Whether activated by CCK-8 or APO, atropine blocked the RGC but not the post-RGC contractions. Domperidone blocked all responses to APO but not to CCK-8, and splanchnicectomy did not affect responses to either agent. Vagotomy blocked all gastrointestinal responses to APO but not to CCK-8. These results indicated that CCK-8 activates the gastrointestinal motor and myoelectric correlates of vomiting by a peripheral mechanism that does not include dopamine receptors.

scholarly journals A COMPARATIVE STUDY OF THE ULTRASTRUCTURE OF MICROVILLI IN THE EPITHELIUM OF SMALL AND LARGE INTESTINE OF MICE

1967 ◽  
Vol 34 (2) ◽  
pp. 447-461 ◽  
Author(s):  
T. M. Mukherjee ◽  
A. Wynn Williams
Keyword(s):  
Small Intestine ◽  
Plasma Membrane ◽  
Fine Structure ◽  
Epithelial Cells ◽  
Surface Coat ◽  
Free Zone ◽  
Colonic Crypts ◽  
Mouse Intestine ◽  
Different Levels ◽  
Small And Large Intestine

A comparative analysis of the fine structure of the microvilli on jejunal and colonic epithelial cells of the mouse intestine has been made. The microvilli in these two locations demonstrate a remarkably similar fine structure with respect to the thickness of the plasma membrane, the extent of the filament-free zone, and the characteristics of the microfilaments situated within the microvillous core. Some of the core microfilaments appear to continue across the plasma membrane limiting the tip of the microvillus. The main difference between the microvilli of small intestine and colon is in the extent and organization of the surface coat. In the small intestine, in addition to the commonly observed thin surface "fuzz," occasional areas of the jejunal villus show a more conspicuous surface coat covering the tips of the microvilli. Evidence has been put forward which indicates that the surface coat is an integral part of the epithelial cells. In contrast to the jejunal epithelium, the colonic epithelium is endowed with a thicker surface coat. Variations in the organization of the surface coat at different levels of the colonic crypts have also been noted. The functional significance of these variations in the surface coat is discussed.

scholarly journals The immune response of young turkeys to haemorrhagic enteritis virus infection at different levels and sources of methionine in the diet

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Bartłomiej Tykałowski ◽  
Marcin Śmiałek ◽  
Andrzej Koncicki ◽  
Katarzyna Ognik ◽  
Zenon Zduńczyk ◽  
...  
Keyword(s):  
Immune Response ◽  
Bacterial Infections ◽  
Economic Losses ◽  
Subclinical Disease ◽  
The Usa ◽  
Cell Subpopulations ◽  
Enteritis Virus ◽  
Dietary Treatments ◽  
Positive Effect ◽  
Different Levels

Abstract Background Haemorrhagic enteritis (HE) of turkeys was first described in 1937 in the USA, while in Poland it was first diagnosed in 1987. Polish haemorrhagic enteritis virus (HEV) isolates are usually low pathogenic and trigger a subclinical disease. Unfortunately, even the low- pathogenic HEV strains cause severe immunosuppression leading to secondary bacterial infections and huge economic losses. The objective of this study was to evaluate if the influence of Met on HEV infected turkeys immune response can be differentiated by both its level and source. Met is one of the amino acids that not only play a nutritional role but also participate in and regulate key metabolic pathways and immune response. In our study, the birds were assigned to 4 dietary treatments which differed in Met levels (0.55 and 0.78% in weeks 1–4 of age and 0.45 and 0.65% in weeks 5–8 of age, respectively) and sources (DL-methionine (DLM) or DL-methionine hydroxy analogue (MHA)). Results The HEV added the percentage of CD4+ cells and decreased the percentage of IgM+ cells in the blood, spleen and caecal tonsils (CTs) of turkeys. In addition, it increased the percentage of CD4+CD25+ cells in blood, and interleukin-6 (IL-6) level in plasma. The higher dose of Met led to a significant decrease in the percentages of CD4+, CD8+ and CD4+IL-6+ cell subpopulations in the blood of HEV-infected and uninfected turkeys and to an increase in the percentage of IgM+ B cells in CTs. Turkeys administered feeds with an increased Met content displayed a decrease in plasma IL-6 levels and an increase in plasma IgA levels. Conclusions The results of this study indicate that HEV infection impairs the immune function in turkeys. Met content in the feed has a moderate effect on the immune response in HEV-infected turkeys. The source of this amino acid appears not be as important as its dose, because value of the analysed parameters did not differ significantly between turkeys receiving feeds with DLM or MHA. In the uninfected turkeys, the higher by 40% (than recommended by NRC) level of Met in the feeds had a positive effect on humoral immunity parameters.

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