scholarly journals Detection of Antibiotic Residues in Chicken Meat and Eggs from Traditional Markets at Yogyakarta City Using Bioassay Method

2020 ◽  
pp. 1-6
Author(s):  
Dyah Ayu Widiasih ◽  
Yatri Drastini ◽  
Doddi Yudhabuntara ◽  
F. Lintang R. Daru Maya ◽  
Prisha Lini Sivalingham ◽  
...  
Keyword(s):  
Chicken Meat ◽  
Animal Origin ◽  
Antibiotic Residues ◽  
Traditional Markets ◽  
Bioassay Method ◽  
Food Of Animal Origin ◽  
Meat Samples ◽  
Tetracycline Residues ◽  
Meat And Eggs ◽  
Yogyakarta City

Studies on antibiotic residues content in food of animal origin are currently needed to support veterinary public health programs. The present study was described bioassay method for the detection of antibiotic residues in chicken meat and eggs from traditional market at Yogyakarta City. A number of twenty-four chicken meat samples and 24 egg samples were taken from 8 traditional markets in Yogyakarta city. Samples were examined at Centre for Veterinary Wates, Yogyakarta, Indonesia using bioassay method for screening detection of penicillin, aminoglycoside, macrolide and tetracycline residues. This bioassay method using some bacteria, such as Bacillus stearothermophillus, B. cereus, B. subtilis, and Kocuria rizophila. A percentage of the results showed that 8.33% (2/24) samples of chickens tested positively contained the oxytetracycline antibiotic residues. Meanwhile, as much as 75% (18/24) samples of positive eggs contain penicillin antibiotic residues, positive residues of aminoglycoside amounted to 12.5% (3/24) and the positive residues of oxytetracycline also amounted to 12.5% (3/24).

scholarly journals Deteksi Kejadian Residu Tetrasiklin pada Daging Ikan Nila di Kota Yogyakarta dengan Kromatografi Cair Kinerja Tinggi (KCKT)

2019 ◽  
Vol 37 (2) ◽  
pp. 185
Author(s):  
Wari Pawestri ◽  
Gagak Dhony Satria ◽  
Nisa Hakimah ◽  
Doddi Yudhabuntara
Keyword(s):  
High Performance ◽  
Residue Analysis ◽  
Animal Origin ◽  
Chemical Contamination ◽  
Traditional Markets ◽  
Food Of Animal Origin ◽  
Absolute Requirement ◽  
Antibiotics Residues ◽  
Meat Samples ◽  
Tetracycline Residues

Food products of animal origin which are free from biological and chemical contamination are an absolute requirement of food safety. Antibiotics residues in the food of animal origin is one of the chemical contaminants in food are harmful/hazardous to human health. Tetracycline is an antibiotic that is often used in the fishing industry. The study aims to detect the occurrence of tetracycline residues in tilapia sold in traditional markets in Kota Yogyakarta. The research was conducted with detection test (detect disease). The study used 61 samples of tilapia fish from 16 traditional markets in Kota Yogyakarta. Tilapia meat samples are prepared according to the Association of Official Analytical Chemistry (AOAC). The existence of tetracycline residues in meat can be detected by high performance liquid chromatography (HPLC). Residue analysis in fish meat was conducted at the Department of Pharmacology, Faculty of Veterinary Medicine of Gadjah Mada University using HPLC Shimadzu version 6.1. The results showed that 24 samples of tilapia meat marketed in Kota Yogyakarta was positively contained tetracycline antibiotic residues. A total of 19 samples contained residues above maximum residue limits (MRL). The conclusion of the study is 31% of tilapia marketed in Kota Yogyakarta contains tetracycline residues above MRL. Surveillance of residues in food of animal origin and further research in terms of reducing antibiotic residue in tilapia meat is highly recommended to improve the quality and safety of food of animal origin.

scholarly journals Impact of Antibiotics Residues on Food Microbiological Quality in Togo: Cases of Chicken Meat and Eggs

2020 ◽  
Author(s):  
Banfitebiyi GAMBOGOU ◽  
Abla Judith Estelle GNAMEY ◽  
Raouf T. MENSAH ◽  
Délia Carmen DIABANGOUAYA ◽  
Messanh KANGNI-DOSSOU ◽  
...  
Keyword(s):  
Food Security ◽  
Microbiological Quality ◽  
Chicken Meat ◽  
Test Method ◽  
Animal Origin ◽  
Antibiotic Residues ◽  
Bacteriological Quality ◽  
Antibiotics Residues ◽  
The Impact ◽  
Meat And Eggs

Abstract Purpose: The use of antibiotics in veterinary medicine has greatly promoted the intensification of animal production in recent decades. However, the improper use of these veterinary antibiotics causes their residues to be present in foodstuffs of animal origin and thus creates public health issues. Thus, the aim of this study is to assess the impact of the presence of antibiotic residues on bacteriological quality of meat and chicken eggs in Togo.Methodology: a survey was carried out among hundred chicken farmers in six markets in the city of Lomé. Then, AFNOR's routine method and the rapid Test method (Premi test) were used to assess the bacteriological quality and the presence of antibiotics in Chicken meats (54 samples) and eggs (36 samples). The statistical analysis is done with software SPSS v25.Results: The results of surveys of our operators have shown that broilers and laying hens are the most represented in the six markets. None of the interviewees ever received any training in biosecurity, food security and good hygiene practice. Out of 90 samples analyzed, 18.89% are positive for antibiotic residues with 99.51% of correlation between antibiotic residues and origin of the sample (p<0.05). Regarding microbiological quality, 16,7% (13/78) of compliance sample are positive to antibiotic residues and 33.3% (4/12) of no compliance sample are positive for antibiotic residues with 92.91% of correlation between microbiology quality and antibiotics residues (p <0.05).Conclusion: The presence of antibiotic residues in the chicken meat and eggs influence the detection of microorganisms. This leads to a food security problem in Togo.

scholarly journals Antibiotic resistance and residue in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh

2021 ◽  
Vol 5 (1) ◽  
pp. 19-26
Author(s):  
Bidyut Matubber ◽  
Farzana Islam Rume ◽  
Mohammad Enamul Hoque Kayesh ◽  
Mohammad Mahfuzur Rahman ◽  
Mohammad Rohul Amin ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Chicken Meat ◽  
Human Consumption ◽  
Antibiotic Residues ◽  
Salmonella Spp ◽  
Resistance Patterns ◽  
Cattle Meat ◽  
Meat Samples ◽  
Controlling Measures ◽  
Antibiotic Resistance Patterns

The presence of antibiotic residue in chicken and animal meats is a serious threat to human health due to its harmful effects. This study aimed at identifying the antibiotic resistance patterns of the isolates as well as antibiotic residues in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh. A total of 205 meat samples, including 70 chicken meat, 60 cattle meat, 50 buffalo meat and 25 goat meat were aseptically collected and analysed for the detection of antibiotic residues by thin layer chromatography and the isolates obtained from these samples were subjected to antibiogram study against 16 commonly used antibiotics. The isolates found in this study were Staphylococcus spp., Streptococcus spp., Escherichia coli, and Salmonella spp. and their prevalence were 37.5% (77/205), 22.1% (48/205), 29.7% (61/205), 8.7% (19/205), respectively. The isolates showed different degrees of sensitivity to the antibiotics used in the study. The most resistant phenotype was against cefradine, amoxicillin, penicillin, oxytetracycline, erythromycin, and enrofloxacin. 18.5% (38/205) meat samples were found to be positive for antibiotic residues and the highest prevalence was observed in chicken meat compared to other meat types. Overall, the findings of the study suggest that it is important to take controlling measures for the emergence of antibiotic resistance and also for ensuring healthy meats for human consumption. Asian Australas. J. Food Saf. Secur. 2021, 5 (1), 19-26

scholarly journals Citrobacter braakii Yield False-Positive Identification as Salmonella, a Note of Caution

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2177
Author(s):  
Joanna Pławińska-Czarnak ◽  
Karolina Wódz ◽  
Magdalena Kizerwetter-Świda ◽  
Tomasz Nowak ◽  
Janusz Bogdan ◽  
...  
Keyword(s):  
False Positive ◽  
Salmonella Enterica ◽  
Meat Products ◽  
Animal Origin ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
False Positive Identification ◽  
Food Of Animal Origin ◽  
Meat Samples

Background: Globally, Salmonella enterica is one of the leading causes of foodborne illness in humans. Food of animal origin is obligatorily tested for the presence of this pathogen. Unfortunately, in meat and meat products, this is often hampered by the presence of background microbiota, which may present as false-positive Salmonella. Methods: For the identification of Salmonella spp. from meat samples of beef, pork, and poultry, the authorized detection method is PN-EN ISO 6579-1:2017-04 with the White–Kauffmann–Le Minor scheme, two biochemical tests: API 20E and VITEK II, and a real-time PCR-based technique. Results: Out of 42 presumptive strains of Salmonella, 83.3% Salmonella enterica spp. enterica, 14.3% Citrobacter braakii, and 12.4% Proteus mirabilis were detected from 180 meat samples. Conclusions: Presumptive strains of Salmonella should be identified based on genotypic properties such as DNA-based methods. The aim of this study was the isolation and identification of Salmonella spp. from miscellaneous meat sorts: beef, pork, and poultry.

scholarly journals Effect of doxycycline concentrations in chicken tissues as a consequence of permanent exposure to enrofloxacin traces in drinking water

2016 ◽  
Vol 60 (3) ◽  
pp. 293-299 ◽  
Author(s):  
Małgorzata Gbylik-Sikorska ◽  
Andrzej Posyniak ◽  
Tomasz Śniegocki ◽  
Bartosz Sell ◽  
Anna Gajda ◽  
...  
Keyword(s):  
Antibacterial Agents ◽  
Animal Origin ◽  
Water Supply Systems ◽  
Antibiotic Residues ◽  
Withdrawal Time ◽  
Antibacterial Compounds ◽  
Chicken Tissues ◽  
Food Of Animal Origin ◽  
Supply Systems ◽  
Poultry Farming

AbstractIntroduction: The main problem in poultry farming is the difficulty in producing food of animal origin without using antibacterial agents. Because most antibacterial compounds are dispensed in water, some water supply systems can be contaminated by antibiotics which are then administered to the animals unintentionally. This can lead to unexpected increases in antibiotic residues in food of animal origin. The aim of the present study was to determine whether the constant exposure of chicken broilers to enrofloxacin affects the withdrawal time of a therapeutic doxycycline that is intentionally administered to the chickens.Material and Methods: The concentrations of doxycycline, enrofloxacin, and ciprofloxacin were determined by LC-MS/MS in muscles and liver of the chickens.Results: Doxycycline residue concentrations in the chicken tissues from the group that received trace amounts of enrofloxacin were nearly 50% greater than those of the group that received only doxycycline.Conclusion: These results indicated that constant exposure to enrofloxacin in trace amounts significantly influences the residual doxycycline concentration in chicken tissues.

scholarly journals Kajian Cemaran Salmonella spp pada Daging Ayam Broiler di Pasar Tradisional dan Modern Kota Bandar Lampung

2021 ◽  
pp. 1-4
Author(s):  
Z Zairiful ◽  
Y Sukaryana ◽  
K Maghfiroh
Keyword(s):  
Broiler Chicken ◽  
Chicken Meat ◽  
Personal Hygiene ◽  
Pathogenic Microorganisms ◽  
Salmonella Spp ◽  
Food Ingredient ◽  
Traditional Markets ◽  
Chicken Carcass ◽  
Sterile Plastic ◽  
Meat Samples

Food can be a carrier for pathogenic agents that can disease in consumers (foodborne disease). Chicken meat is one of the products from livestock which has a high consumption rate, because besides being easy to obtain, the growth of chickens is fast, and the price is also more affordable compared to large livestock types. Chicken meat is an excellent medium for microbial growth and makes it a perishable food ingredient. Foodborne illness is a disease caused by pathogenic microorganisms that contaminate food, such as Salmonella spp, Staphylococcus aureus, and Escherichia coli (E.coli). Salmonella spp infection can cause salmonellosis which irritates the digestive tract and many of them result in death. This study aims to assess the presence and number of pathogenic microorganisms Salmonella spp in broiler chicken sold in traditional and modern markets in Bandar Lampung. The research was conducted by purposive sampling of 30 chicken meat samples. Broiler chicken carcass samples were taken as whole and then stored in sterile plastic, labeled and put into a cool box filled with ice. The variables observed in this study were the presence and number of Salmonella bacteria. The data obtained were analyzed descriptively. The results of Salmonella spp examination on chicken meat samples in traditional markets in Bandar Lampung City showed a positive  in 1 (one) sample or 6.7%, while in modern markets Salmonella was not found. The presence of Salmonella in chicken meat is thought to be due to contamination from the water used during the process of slaughtering and preparing the carcass, contamination from the carcass slaughtering and selling environment which does not apply good sanitary hygiene and poor personal hygiene of the traders. The conclusion obtained is that the number of Salmonella spp in chicken meat in traditional markets is not in accordance with SNI 7388 of 2009 concerning the Maximum Limit of Microbial Contamination (BMCM) in fresh meat.

scholarly journals Determination of Polypeptide Antibiotic Residues in Food of Animal Origin by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3261
Author(s):  
Tomasz Bladek ◽  
Iwona Szymanek-Bany ◽  
Andrzej Posyniak
Keyword(s):  
High Performance ◽  
Multiple Reaction Monitoring ◽  
Gradient Elution ◽  
Animal Origin ◽  
Antibiotic Residues ◽  
Triple Quadrupole Mass Spectrometer ◽  
Limit Of Quantification ◽  
Food Of Animal Origin ◽  
Polypeptide Antibiotic

A novel UHPLC-MS/MS method for the determination of polypeptide antibiotic residues in animal muscle, milk, and eggs was developed and validated. Bacitracin A, colistin A, colistin B, polymyxin B1, and polymyxin B2 were extracted from the samples with a mixture of acetonitrile/water/ammonia solution 25%, 80/10/10 (v/v/v), and put through further evaporation, reconstitution, and filtration steps. The chromatographic separation was performed on a C18 column in gradient elution mode. Mass spectral acquisitions were performed in selective multiple reaction monitoring mode by a triple quadrupole mass spectrometer. The method was validated according to the criteria of Commission Decision 2002/657/EC. The method quantifies polypeptides in a linear range from 10 to 1000 μg kg−1, where the lowest concentration on the calibration curve refers to the limit of quantification (LOQ). The recoveries ranged from 70 to 99%, the repeatability was below 13%, and within-laboratory reproducibility was lower than 15%. The decision limit (CCα) and detection capability (CCβ) values were calculated, and ruggedness and stability studies were performed, to fulfill the criteria for confirmatory methods. Moreover, the developed method may also be used for screening purposes by its labor efficiency.

scholarly journals Selenium in poultry nutrition: a review

2014 ◽  
Vol 59 (No. 11) ◽  
pp. 495-503 ◽  
Author(s):  
P. Suchý ◽  
E. Straková ◽  
I. Herzig
Keyword(s):  
Antioxidant Activity ◽  
Functional Foods ◽  
Egg Quality ◽  
Biological Activities ◽  
Poultry Meat ◽  
Animal Origin ◽  
Direct Link ◽  
Food Of Animal Origin ◽  
Meat And Eggs ◽  
Cattle And Sheep

Selenium has been known for two centuries and its biological activities have been studied for nearly a hundred years, however the problem of Se deficit has still been persisting both in humans and animals. The cause of Se deficit in animals may be low Se level in soil. This mainly applies to cattle and sheep with their direct link to soil via roughage. The risk of Se deficit in pigs and poultry is lower due to lower dependence on soil Se level in the region and Se fortification in feed mixes. The present research focuses on the effects of Se on meat and egg quality, antioxidant activity of Se, effects on fatty acid levels, activity of glutathione-peroxidase, or the effect of Se on the immune system. Ensuring natural Se supply in human nutrition by food of animal origin, mainly poultry meat and eggs (the &ldquo;functional foods&rdquo;), is another area to which extensive attention has recently been paid. &nbsp;

High Level of Antimicrobial Resistance in Campylobacter jejuni Isolated from Broiler Chickens in Estonia in 2005 and 2006

2007 ◽  
Vol 70 (8) ◽  
pp. 1940-1944 ◽  
Author(s):  
MATI ROASTO ◽  
KADRIN JUHKAM ◽  
TERJE TAMME ◽  
ARI HÖRMAN ◽  
LIIDIA HÄKKINEN ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Broiler Chickens ◽  
Animal Origin ◽  
Campylobacter Coli ◽  
National Veterinary Institute ◽  
Food Of Animal Origin ◽  
Meat Samples ◽  
High Level

The development of antimicrobial resistance in Campylobacter jejuni and Campylobacter coli is a matter of increasing concern. Because campylobacteriosis is transmitted to humans usually via food of animal origin, the presence of antimicrobial-resistant campylobacters in broiler chickens has important public health implications. The aim of our study was to analyze resistance patterns of C. jejuni isolated from fecal samples collected at a large Estonian chicken farm, from cecal contents collected at slaughterhouses, and from meat samples collected at the retail establishments in 2005 and 2006. A total of 131 C. jejuni isolates were collected over a 13-month period and tested by the broth microdilution VetMIC method (National Veterinary Institute, Uppsala, Sweden) to determine the MICs of various antimicrobials. Resistance to one or more antimicrobials was detected in 104 (79.4%) of the 131 isolates. High proportions of the isolates were resistant to enrofloxacin (73.3%) and nalidixic acid (75.6%). Multidrug resistance (resistance to three or more unrelated antimicrobials) was detected in 36 isolates (27.5%), all of which were resistant to enrofloxacin. Multidrug resistance was significantly associated with enrofloxacin resistance (P &lt; 0.01), and the use of enrofloxacin may select for multiresistant strains.

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