scholarly journals Detection of antifungal compounds in Arabidopsis thaliana and brassica oleracea by thin layer chromatography .

2017 ◽  
Vol 23 (1&2) ◽  
pp. 123
Author(s):  
K.P. Chong ◽  
J.W. Mansfield ◽  
M.H. Bennet ◽  
J.F. Rossiter
Keyword(s):  
Arabidopsis Thaliana ◽  
Thin Layer ◽  
Brassica Oleracea ◽  
Thin Layer Chromatography ◽  
Pseudomonas Syringae ◽  
Antifungal Compounds ◽  
Zone Of Inhibition ◽  
Layer Chromatography

Thin Layer Chromatography (TLC) was applied to detect antifungal compounds both in Arabidopsis thaliana and Brassica oleracea after elicited by various biotic and abiotic elicitors.From TLC bioassays the only strong zone of inhibition detected after challenged by Pseudomonas syringae pv.maculicola was from Arabidopsis tissue and later confirmed by spectrophotometry as camalexin but no corresponding phytoalexin was found in broccoli leaf.

Antifungal compounds in aspen: effect of water stress

1994 ◽  
Vol 72 (4) ◽  
pp. 454-460 ◽  
Author(s):  
Brian M. Kruger ◽  
Paul D. Manion
Keyword(s):  
Tissue Culture ◽  
Water Stress ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Clonal Variation ◽  
Antifungal Compounds ◽  
Inhibitory Compounds ◽  
Hypoxylon Mammatum ◽  
Layer Chromatography ◽  
Tissue Culture Plantlets

A thin layer chromatography bioassay was used to detect antifungal compounds in tissue culture plantlets and potted seedlings of aspen. Catechol and the phenolic glycosides salicin and salicortin were identified as inhibitory compounds; a fourth compound was observed but was not identified. Inhibitory compound levels were estimated in eight tissue culture clones grown on unamended media and media amended with 0.22 M mannitol to induce water stress. Aspen tissue culture plantlets grown under water stress conditions had significantly lower levels of catechol, salicortin, and salicin. Significant clonal variation in levels of catechol, salicin, and the unidentified compound was also observed. Catechol, salicortin, and salicin were inhibitory to Hypoxylon mammatum when tested at levels similar to those employed in the thin layer chromatography bioassay. These results suggest that a reduction in the levels of inhibitory compounds in water-stressed aspen may be a factor in the water stress induced susceptibility of aspen to H. mammatum. Key words: Populus tremuloides, Hypoxylon mammatum, water stress, tissue culture.

scholarly journals In Vitro Antibacterial Activity of Spanish Moss (Tillandsia usneoides) Crude Extract Against Skin Infection in Wound Healing

2017 ◽  
Vol 9 (10) ◽  
Author(s):  
Faller E. M. ◽  
Kanes S. N. ◽  
Zajmi A. ◽  
Ramli M. D.
Keyword(s):  
Wound Healing ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pathogenic Bacteria ◽  
Methanolic Extract ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Tillandsia Usneoides ◽  
Layer Chromatography

Wound healing is a dynamic phenomenon that results in the restoration of anatomic continuity and function on which can be delayed by pathogenic bacteria. This research was designed to explore the antimicrobial efficacy of Tillandsia usneoides against skin infections in wound healing. Physical and chemical evaluations were done through phytochemical screening and thin layer chromatography. The effect of methanolic, ethanolic and aqueous extracts of Tillandsia usneoides against pathogenic bacteria were evaluated on antimicrobial activities using disc diffusion and broth dilution susceptibility assay. The excision bioassay analysis was used in examining the wound healing process in mice. The result of the qualitative phytochemical screening showed the presence of flavonoids and alkaloids. Thin layer chromatography revealed a high Rf value for flavonoids (0.75mm) and alkaloids (0.60mm). The antibacterial assay showed a high zone of inhibition (ZI) for both methanolic extract (>23mm, >22mm and >20mm) and ethanolic extract (>22mm, >22mm and >17mm) for P.aeruginosa, S.aureus and S.epidermis respectively. Resistance against aqueous extract was observed based on the lowest zone of inhibition ( less than 4mm). Gentamicin was used as the positive control (>28mm) and DMSO as the negative control. The minimum inhibitory concentration and minimum bactericidal concentration results confirmed that methanolic and ethanolic extracts restrained the growth of tested bacteria in the range of 125 to 500mg/mL and showed bactericidal efficacy. Wound healing assay indicated that methanolic extract had a higher potency of wound closure (12 days; less than 1mm) compared to ethanolic and aqueous extracts (13days; >1.5mm and 13days: >1.8mm respectively). Povidone-Iodine was used as the gold standard (15days; less than 2mm) in the study. In conclusion, methanolic extract of T. usneoides has a great potential with regard to its antimicrobial and wound healing activity to be developed as a novel drug in the future.

Determination of Vancomycin in Lobster Meat

1980 ◽  
Vol 37 (6) ◽  
pp. 1038-1040 ◽  
Author(s):  
Doris V. Herbst
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chromatographic Method ◽  
Zone Of Inhibition ◽  
Presumptive Identification ◽  
Residue Levels ◽  
Layer Chromatography

Residues of vancomycin in cooked lobster meat were detected by using a thin-layer chromatographic method with bioautography. Presumptive identification was provided by the Rf value coupled with the unique zone of inhibition, and residue levels of 5 μg/g could be detected. The procedure provides methodology needed for regulatory purposes.Key words: lobster meat, vancomycin, bioautography, thin-layer chromatography

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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