Determination of Vancomycin in Lobster Meat

1980 ◽  
Vol 37 (6) ◽  
pp. 1038-1040 ◽  
Author(s):  
Doris V. Herbst
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chromatographic Method ◽  
Zone Of Inhibition ◽  
Presumptive Identification ◽  
Residue Levels ◽  
Layer Chromatography

Residues of vancomycin in cooked lobster meat were detected by using a thin-layer chromatographic method with bioautography. Presumptive identification was provided by the Rf value coupled with the unique zone of inhibition, and residue levels of 5 μg/g could be detected. The procedure provides methodology needed for regulatory purposes.Key words: lobster meat, vancomycin, bioautography, thin-layer chromatography

Thin Layer Chromatographic Method for Determination of Deoxynivalenol in Wheat: Collaborative Study

1986 ◽  
Vol 69 (1) ◽  
pp. 37-40 ◽  
Author(s):  
Robert M Eppley ◽  
Mary W Trucksess ◽  
Stanley Nesheim ◽  
Charles W Thorpe ◽  
Albert E Pohland ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Aluminum Chloride ◽  
Chloride Solution ◽  
Chromatographic Method ◽  
Collaborative Study ◽  
Sample Extraction ◽  
Layer Chromatography

Abstract A collaborative study of a rapid method for the determination of deoxynivalenol (DON) in winter wheat was successfully completed. The method involves sample extraction with acetonitrile-water (84 + 16), cleanup using a disposable column of charcoal, Celite, and alumina, and detection by thin layer chromatography after spraying with an aluminum chloride solution. Each of the 15 collaborators analyzed 12 samples, 2 of which were naturally contaminated, and 10 to which DON was added, in duplicate, at levels of 0,50,100,300, and 1000 ng/ g. Average recoveries of DON ranged from 78 to 96% with repeatabilities of 30-64% and reproducibilities of 33-87%. The results of the study show that false positives were not a problem and that all of the analysts could detect DON at the 300 ng/g level or higher. The method has been adopted official first action.

Collaborative Study of a Chromatographic Method for Determination of Patulin in Apple Juice

1974 ◽  
Vol 57 (3) ◽  
pp. 621-625 ◽  
Author(s):  
Peter M Scott
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Apple Juice ◽  
Chromatographic Method ◽  
Collaborative Study ◽  
Spray Reagent ◽  
Contamination Levels ◽  
Layer Chromatography

Abstract Collaborators in 20 laboratories studied a method for the determination of patulin in apple juice. The collaborators mixed patulin solutions with the apple juice to obtain 9 spiked samples (of which 2 were blanks) and 1 practice sample. After column chromatography of the apple juice extract, patulin was estimated visually by thin layer chromatography, using 3-methyl-2-benzothiazolinone hydrazone.HCl as the spray reagent. Mean recoveries of patulin ranged from 69.8 to 98.3% for 3 contamination levels. The method has been adopted as official first action for the determination of patulin in apple juice.

Determination of Glutethimide (Doriden) and Its Metabolites in Biologic Specimens

1968 ◽  
Vol 14 (7) ◽  
pp. 595-609 ◽  
Author(s):  
Irving Sunshine ◽  
Robert Maes ◽  
Rosalie Faracci
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Quantitative Determination ◽  
Thin Layer Chromatography ◽  
Chromatographic Method ◽  
Blood Concentrations ◽  
Gas Chromatographic Method ◽  
Clinical Conditions ◽  
Layer Chromatography

Abstract A gas chromatographic method for the quantitative determination of glutethimide was developed. This method was applied to samples obtained from patients who were comatose because of acute glutethimide intoxication. The results obtained from this procedure were compared with those obtained by using an ultraviolet method. The patients’ clinical conditions were assessed and correlated with the blood concentrations of glutethimide. The samples were processed to isolate glutethimide, as well as its metabolites. The metabolites were separated using thin-layer chromatography and were checked for purity by both thin-layer and gas chromatography.

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