scholarly journals Biofilms of Azospirillum brasilense and Azospirillum lipoferum and their resistance to abiotic stresses

2020 ◽  
Author(s):  
S. S. Yevstigneyeva ◽  
Yu. P. Fedonenko ◽  
A. V. Shelud’ko
Keyword(s):  
Abiotic Stresses ◽  
Azospirillum Brasilense ◽  
Azospirillum Lipoferum

The biofilms of the bacteria A. brasilense and A. lipoferum are resistant to salt (over 400 mM NaCl in the medium) and temperature (up to 48° C) stresses. The nature of resistance to these stressors is determined by the type of biofilm.

Characterization of Azospirillum associated with maize (Zea mays) in France, using biochemical tests and plasmid profiles

1992 ◽  
Vol 38 (8) ◽  
pp. 798-803 ◽  
Author(s):  
Isabelle Penot ◽  
Nathalie Berges ◽  
Christine Guinguene ◽  
Jacques Fages
Keyword(s):  
Zea Mays ◽  
Azospirillum Brasilense ◽  
Zea Mays L ◽  
Specific Method ◽  
Azospirillum Lipoferum ◽  
Biochemical Tests ◽  
Plasmid Profiles ◽  
Clear Differentiation ◽  
Plasmid Profiling

Thirty Azospirillum strains were isolated from the rhizosphere of 13 maize (Zea mays L.) cultivars grown in 14 French soils, using a new specific method, which has been given the name ROSEA. Among these strains 26 were Azospirillum lipoferum and 4 were Azospirillum brasilense. Their characterization was achieved using biochemical tests and plasmid profiles. Biochemical patterns allowed clear differentiation between the two species. A large diversity in carbon source metabolism was found among the Azospirillum sp. strains regardless of their origin. The A. brasilense were much more closely related, and were found in only two of the rhizospheres studied. The 30 plasmid patterns were all different, and the plasmid-profiling technique can therefore be considered as strain specific. All the A. lipoferum harboured a 150-MDa plasmid, while all the A. brasilense harboured a 90 to 100-MDa plasmid. This result reinforces the hypothesis of the presence of such plasmids as an additional criterion for differentiating these two species. Key words: Zea mays, Azospirillum, ROSEA method, biochemical tests, plasmid profiles.

scholarly journals Realocação de nitrogênio e de biomassa para os grãos, em trigo submetido a inoculação de Azospirillum

2000 ◽  
Vol 35 (2) ◽  
pp. 401-411 ◽  
Author(s):  
AGOSTINHO DIRCEU DIDONET ◽  
OSVALDO DOS SANTOS LIMA ◽  
ANDRÉ ALESSANDRO CANDATEN ◽  
OSMAR RODRIGUES
Keyword(s):  
Triticum Aestivum ◽  
Azospirillum Brasilense ◽  
Triticum Aestivum L ◽  
Azospirillum Lipoferum

Estudou-se, em condições de campo, o efeito de inoculante turfoso em pó contendo bactérias do gênero Azospirillum no rendimento de grãos e na remobilização de N e de biomassa para os grãos de trigo (Triticum aestivum L.), cultivar Embrapa 16. Usaram-se como inoculantes, a estirpe de Azospirillum brasilense 245 e o isolado 10 de Azospirillum lipoferum. Em cada tratamento de inoculação, e também sem inoculação, aplicaram-se diferentes doses de N em diferentes estádios de desenvolvimento das plantas, distribuídos em blocos ao acaso com parcelas subdivididas. Na antese e na maturação fisiológica, avaliaram-se o acúmulo de massa seca e N total nas diferentes partes da planta. Na colheita, além do rendimento de grãos, avaliaram-se também o índice de colheita para biomassa e para N, os principais componentes do rendimento e o teor de N total de grãos. Os resultados ainda preliminares evidenciaram que, mesmo não havendo efeito da inoculação no rendimento de grãos, houve um melhor alocamento de N e de biomassa para os grãos, resultando em maior massa de mil grãos e em menor quantidade de N restante na palha das plantas na maturação fisiológica. Esses efeitos resultaram de um menor número de espigas m-2, provavelmente devido à morte de afilhos, fator que determinou maior disponibilidade de N e de biomassa às espigas e grãos restantes.

Pectin decomposition and associated nitrogen fixation by mixed cultures of Azospirillum and Bacillus species

1992 ◽  
Vol 38 (8) ◽  
pp. 794-797 ◽  
Author(s):  
K. M. Khammas ◽  
P. Kaiser
Keyword(s):  
Nitrogen Fixation ◽  
Azospirillum Brasilense ◽  
Nitrogenase Activity ◽  
Bacillus Species ◽  
Azospirillum Lipoferum ◽  
Pectin Degradation ◽  
Efficient Utilization ◽  
Fermentation Products ◽  
Pure Cultures ◽  
Azospirillum Amazonense

Cocultures of different Azospirillum species with Bacillus polymyxa or Bacillus subtilis allow the efficient utilization of pectin as carbon and energy sources for nitrogen fixation. The nitrogenase activity obtained with cocultures was as high as 30–80 nmol C2H4 h−1 mL−1, a much higher value than that obtained with pure cultures of either Azospirillum (up to 13 nmol C2H4 h−1 mL−1) or B. polymyxa (up to 2 nmol C2H4 h−1 mL−1) alone. To establish to what extent each partner contributed to nitrogenase activity, acetylene reduction was assayed as a function of time and it was also measured on Azospirillum cultivated in the cultures filtrates of the Bacillus. The results suggested that the nitrogenase activity was mostly produced by Azospirillum. The nitrogenase activity occurred at the expense of the degradation and fermentation products of the pectin. The new pectinolytic species, Azospirillum irakense, utilized both degradation and fermentation products of pectin, whereas the nonpectinolytic strains (Azospirillum brasilense, Azospirillum lipoferum, Azospirillum amazonense) utilized only the fermentation products of pectin, including acetic and succinic acids. These cocultures can be considered as metabolic associations, where the Bacillus produces degradation and fermentation products of pectin, which can be used by Azospirillum species. Key words: cocultures, nitrogen fixation, pectin degradation, Azospirillum, Bacillus, metabolic association.

Homology between plasmids of Azospirillum brasilense and Azospirillum lipoferum

1989 ◽  
pp. 165-172 ◽  
Author(s):  
Claire Vieille ◽  
I. Onyeocha ◽  
M. Galimand ◽  
Claudine Elmerich
Keyword(s):  
Azospirillum Brasilense ◽  
Azospirillum Lipoferum

Effect of Azospirillum Inoculation on Growth and Yield of Lentil

1970 ◽  
Vol 24 (1) ◽  
pp. 30-33
Author(s):  
M Motiur Rahman ◽  
Sanzida Mubassara ◽  
Sirajul Hoque ◽  
Zahed UM Khan
Keyword(s):  
Field Experiment ◽  
Azospirillum Brasilense ◽  
Growth Yield ◽  
Dry Weight ◽  
Grain Weight ◽  
Growth And Yield ◽  
Fresh Weight ◽  
Azospirillum Lipoferum ◽  
Lens Esculenta ◽  
Stages Of Growth

A field experiment was conducted in 2005 to evaluate the effect of some isolates of Azospirillum brasilense and Azospirillum lipoferum on growth and yield of lentil (Lens esculenta). Azospirillum inoculation significantly enhanced the early emergence of lentil seeds in the field. The inoculation caused the increase in height of the lentil plants at various stages of growth. Due to inoculation fresh weight and dry weight of lentil plant were increased during pre-flowering and post-flowering stages. Significant increase in the number of pods per plant and grain weight due to Azospirillum inoculation was observed. It was also found that the population of Azospirillum increased significantly in the inoculated root samples of lentil plants. Keywords: Azospirillum, Inoculation, Growth, Yield, Lentil (Lens esculenta)DOI: http://dx.doi.org/10.3329/bjm.v24i1.1233 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp.30-33

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