Characterization of Azospirillum associated with maize (Zea mays) in France, using biochemical tests and plasmid profiles

1992 ◽  
Vol 38 (8) ◽  
pp. 798-803 ◽  
Author(s):  
Isabelle Penot ◽  
Nathalie Berges ◽  
Christine Guinguene ◽  
Jacques Fages

Thirty Azospirillum strains were isolated from the rhizosphere of 13 maize (Zea mays L.) cultivars grown in 14 French soils, using a new specific method, which has been given the name ROSEA. Among these strains 26 were Azospirillum lipoferum and 4 were Azospirillum brasilense. Their characterization was achieved using biochemical tests and plasmid profiles. Biochemical patterns allowed clear differentiation between the two species. A large diversity in carbon source metabolism was found among the Azospirillum sp. strains regardless of their origin. The A. brasilense were much more closely related, and were found in only two of the rhizospheres studied. The 30 plasmid patterns were all different, and the plasmid-profiling technique can therefore be considered as strain specific. All the A. lipoferum harboured a 150-MDa plasmid, while all the A. brasilense harboured a 90 to 100-MDa plasmid. This result reinforces the hypothesis of the presence of such plasmids as an additional criterion for differentiating these two species. Key words: Zea mays, Azospirillum, ROSEA method, biochemical tests, plasmid profiles.

1980 ◽  
Vol 26 (3) ◽  
pp. 291-296 ◽  
Author(s):  
Peter P. Wong ◽  
Nancy E. Stenberg ◽  
Linda Edgar

A bacterium with the taxonomic characteristics of the genus Azospirillum was isolated from cellulolytic N2-fixing mixed cultures. Its characteristics fit the descriptions of both Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. It may be a variant strain of A. lipoferum. In mixed cultures with cellulolytic organisms, the bacterium grew and fixed N2 with cellulose as a sole source of energy and carbon. The mixed cultures used cellulose from leaves of wheat (Triticum aestivum L.), corn (Zea mays L.), and big bluestem grass (Andropogon gerardii Vitm). Microaerophilic N2-fixing bacteria of the genus Azospirillum, such as the bacterium we isolated, may be important contributors of fixed N2 in soil with partial anaerobiosis and cellulose decomposition.


2008 ◽  
Vol 8 (1) ◽  
pp. 2 ◽  
Author(s):  
Jeppe Andersen ◽  
Imad Zein ◽  
Gerhard Wenzel ◽  
Birte Darnhofer ◽  
Joachim Eder ◽  
...  

LWT ◽  
2019 ◽  
Vol 101 ◽  
pp. 812-818 ◽  
Author(s):  
Milena Figueiredo de Sousa ◽  
Rafaiane Macedo Guimarães ◽  
Marcos de Oliveira Araújo ◽  
Keyla Rezende Barcelos ◽  
Nárgella Silva Carneiro ◽  
...  
Keyword(s):  
Zea Mays ◽  

2014 ◽  
Vol 175 (3) ◽  
pp. 1344-1357 ◽  
Author(s):  
Qing Dong ◽  
Haiyang Jiang ◽  
QianQian Xu ◽  
Xiaoming Li ◽  
Xiaojian Peng ◽  
...  
Keyword(s):  
Zea Mays ◽  

2008 ◽  
Vol 7 (4) ◽  
pp. 641-650 ◽  
Author(s):  
J.M. Matheka ◽  
E. Magiri ◽  
A.O. Rasha ◽  
J. Machuka

2013 ◽  
Vol 54 (9) ◽  
pp. 1515-1524 ◽  
Author(s):  
Riliang Gu ◽  
Fengying Duan ◽  
Xia An ◽  
Fusuo Zhang ◽  
Nicolaus von Wirén ◽  
...  

2017 ◽  
Vol 17 (3) ◽  
pp. 14 ◽  
Author(s):  
Marcos Vinícius Ribas Milleo ◽  
Isadora Cristófoli Pereira

Com o objetivo de avaliar a eficiência agronômica da inoculação com AzzoFix® sobre a cultura do milho (Zea mays L.) foram conduzidos experimentos nas cidades de Ponta Grossa e Palmeira - PR, na safra de 2013/2014. O delineamento experimental foi em blocos ao acaso, com oito tratamentos e quatro repetições. Os tratamentos consistiram em: 1- Testemunha; 2- 50% da dose de nitrogênio (N) recomendada para a cultura e inoculação das sementes com AzoTotal (150 mL 25 kg-1 de sementes); 3- Dose de N recomendada; 4- 50% da dose de N recomendada; 5- Inoculação com Azzofix (100 mL 25 kg de sementes-1); 6-  Azzofix (100 mL 25 kg de sementes-1) + 50% da dose de N recomendada; 7- Azzofix (150 mL 25 kg de sementes-1) e 8- Azzofix (150 mL 25 kg de sementes-1) + 50% da dose de N recomendada (semeadura). Avaliou-se a massa seca de folhas e de raízes (g), o N total na massa seca (N g kg-1), o N total nos grãos (% de N) e o rendimento da cultura em kg ha-1. A inoculação das sementes de milho com Azzofix pode assegurar redução nos custos de produção com incremento no rendimento de grãos da cultura. 


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