scholarly journals Detection and primary characterization of Rothia amarae in a suspension culture of Arabidopsis thaliana (Heynh.) cells

2020 ◽  
Author(s):  
A. O. Sokolov ◽  
L. A. Dykman ◽  
A. A. Galitskaya ◽  
O. I. Sokolov
Keyword(s):  
Arabidopsis Thaliana ◽  
16S Rrna ◽  
Suspension Culture ◽  
Plant Cells ◽  
16S Rrna Sequencing ◽  
Rrna Sequencing ◽  
Long Time ◽  
Immunochemical Methods

The presence of non-pathogenic bacterial microflora in Arabidopsis thaliana suspension culture has been shown to coexist latently with plant cells for a long time. According to the results of 16S rRNA sequencing, it was shown that the detected bacteria belong to the species Rothia amarae. Using microbiological, microscopic and immunochemical methods, the correspondence of the obtained strain to the species Rothia amarae was also confirmed.

Characterization of bovine ruminal epithelial bacterial communities using 16S rRNA sequencing, PCR-DGGE, and qRT-PCR analysis

2012 ◽  
Vol 155 (1) ◽  
pp. 72-80 ◽  
Author(s):  
Meiju Li ◽  
Mi Zhou ◽  
Elizabeth Adamowicz ◽  
John A. Basarab ◽  
Le Luo Guan
Keyword(s):  
16S Rrna ◽  
Bacterial Communities ◽  
16S Rrna Sequencing ◽  
Pcr Analysis ◽  
Qrt Pcr ◽  
Rrna Sequencing

scholarly journals Characterization of bacterial communities in wastewater with enhanced taxonomic resolution by full-length 16S rRNA sequencing

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Daniela Numberger ◽  
Lars Ganzert ◽  
Luca Zoccarato ◽  
Kristin Mühldorfer ◽  
Sascha Sauer ◽  
...  
Keyword(s):  
16S Rrna ◽  
Bacterial Communities ◽  
Full Length ◽  
16S Rrna Sequencing ◽  
Taxonomic Resolution ◽  
Rrna Sequencing

scholarly journals Characterization of Gut Microbiota in Prenatal Cold Stress Offspring Rats by 16S rRNA Sequencing

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1619
Author(s):  
Jiasan Zheng ◽  
Tingting Zhu ◽  
Lipeng Wang ◽  
Jianfa Wang ◽  
Shuai Lian
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
Cold Stress ◽  
Female Offspring ◽  
16S Rrna Sequencing ◽  
Negative Effects ◽  
Rrna Sequencing ◽  
Temperature Group ◽  
Microbial Dna

Our previous study showed a reduction of anxiety-like behavior in offspring rats suffered from prenatal cold stress; whether this was related to changes in the offspring gut microbiota is unclear. To obtain the evidence for the role of the gut microbiota in prenatal cold stress offspring, 16S rRNA sequencing technology was used. Male and female offspring rat feces were collected from a room temperature group and a prenatal cold stress group (n ≥ 8) for microbial DNA extraction, followed by 16S rRNA sequencing. The results indicated that prenatal cold stress could change the offspring’s gut microbiota composition. Prenatal cold stress significantly upregulates Lactobacillus, Lactobacillus_gasseri, Bacteroides, and Bacteroides-acidifaciens in female offspring, whereas prenatal cold stress significantly reduced Lachnospiraceae and Prevotellaceae in male offspring. These data showed the characterization of gut microbiota in prenatal cold stress offspring rats, and these data suggest that microbiological intervention in the future can potentially prevent the negative effects caused by cold stress to animals.

scholarly journals Short Communication: Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing

2019 ◽  
Vol 20 (5) ◽  
Author(s):  
YUNI PUJI HASTUTI ◽  
IMAN RUSMANA ◽  
KUKUH NIRMALA ◽  
RIDWAN AFFANDI ◽  
SISKA TRIDESIANTI
Keyword(s):  
16S Rrna ◽  
16S Rrna Sequencing ◽  
Scylla Serrata ◽  
Nitrifying Bacteria ◽  
Mud Crab ◽  
Aquaculture System ◽  
Rrna Sequencing ◽  
Recirculation Aquaculture System ◽  
Identification And Characterization

Abstract. Hastuti YP, Rusmana I, Nirmala K, Affandi R, Tridesianti S. 2019. Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing. Biodiversitas 20: 1339-1343. Recirculation is one of the systems used for increasing aquaculture production, for example, is mud crab Scylla serrata culture. Aquaculture activities will produce organic nitrogen waste and can be affected to inorganic condition (nitrification bacteria). Nitrifying bacteria are capable of changing aerobically from NH4+ to NO2-(nitrite) and from NO2-to NO3-(nitrate). This current work aimed to identify and characterize nitrifying bacteria in mud crab (S. serrata) culture with a recirculated system. Nitrifying bacteria were isolated using serial dilution (10-1 until 10-5) of open filter FB water sample previously cultured in a specific medium. Bacterial identification was performed using a procedure of PrestoTM Mini gDNA Kit (Geneid) for DNA extraction and Polymerase Chain Reaction (PCR) primer 63F and 1387R for 16S rRNA gene amplification. Molecular identification using 16S rRNA sequences, the nitrifying bacteria isolated from mud crab recirculation aquaculture system were 99% identified as a group Pantoea calida, Pseudomonas stutzerii dan Halomonas sp. Morphological characterization of the colony morphology showed that a mucoid appearance and appeared cream in color and large size. Furthermore, their cells were recognized as basil and Gram-negative.

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