scholarly journals Characterization of Gut Microbiota in Prenatal Cold Stress Offspring Rats by 16S rRNA Sequencing

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1619
Author(s):  
Jiasan Zheng ◽  
Tingting Zhu ◽  
Lipeng Wang ◽  
Jianfa Wang ◽  
Shuai Lian
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
Cold Stress ◽  
Female Offspring ◽  
16S Rrna Sequencing ◽  
Negative Effects ◽  
Rrna Sequencing ◽  
Temperature Group ◽  
Microbial Dna

Our previous study showed a reduction of anxiety-like behavior in offspring rats suffered from prenatal cold stress; whether this was related to changes in the offspring gut microbiota is unclear. To obtain the evidence for the role of the gut microbiota in prenatal cold stress offspring, 16S rRNA sequencing technology was used. Male and female offspring rat feces were collected from a room temperature group and a prenatal cold stress group (n ≥ 8) for microbial DNA extraction, followed by 16S rRNA sequencing. The results indicated that prenatal cold stress could change the offspring’s gut microbiota composition. Prenatal cold stress significantly upregulates Lactobacillus, Lactobacillus_gasseri, Bacteroides, and Bacteroides-acidifaciens in female offspring, whereas prenatal cold stress significantly reduced Lachnospiraceae and Prevotellaceae in male offspring. These data showed the characterization of gut microbiota in prenatal cold stress offspring rats, and these data suggest that microbiological intervention in the future can potentially prevent the negative effects caused by cold stress to animals.

scholarly journals Antibiotic-Induced Dysbiosis of Microbiota Promotes Chicken Lipogenesis by Altering Metabolomics in the Cecum

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 487
Author(s):  
Tao Zhang ◽  
Hao Ding ◽  
Lan Chen ◽  
Yueyue Lin ◽  
Yongshuang Gong ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
High Performance ◽  
Fat Deposition ◽  
16S Rrna Sequencing ◽  
Oral Antibiotics ◽  
Ms Analysis ◽  
Rrna Sequencing ◽  
Cecal Microbiota ◽  
Metabolite Network

Elucidation of the mechanism of lipogenesis and fat deposition is essential for controlling excessive fat deposition in chicken. Studies have shown that gut microbiota plays an important role in regulating host lipogenesis and lipid metabolism. However, the function of gut microbiota in the lipogenesis of chicken and their relevant mechanisms are poorly understood. In the present study, the gut microbiota of chicken was depleted by oral antibiotics. Changes in cecal microbiota and metabolomics were detected by 16S rRNA sequencing and ultra-high performance liquid chromatography coupled with MS/MS (UHPLC–MS/MS) analysis. The correlation between antibiotic-induced dysbiosis of gut microbiota and metabolites and lipogenesis were analysed. We found that oral antibiotics significantly promoted the lipogenesis of chicken. 16S rRNA sequencing indicated that oral antibiotics significantly reduced the diversity and richness and caused dysbiosis of gut microbiota. Specifically, the abundance of Proteobacteria was increased considerably while the abundances of Bacteroidetes and Firmicutes were significantly decreased. At the genus level, the abundances of genera Escherichia-Shigella and Klebsiella were significantly increased while the abundances of 12 genera were significantly decreased, including Bacteroides. UHPLC-MS/MS analysis showed that antibiotic-induced dysbiosis of gut microbiota significantly altered cecal metabolomics and caused declines in abundance of 799 metabolites and increases in abundance of 945 metabolites. Microbiota-metabolite network revealed significant correlations between 4 differential phyla and 244 differential metabolites as well as 15 differential genera and 304 differential metabolites. Three metabolites of l-glutamic acid, pantothenate acid and N-acetyl-l-aspartic acid were identified as potential metabolites that link gut microbiota and lipogenesis in chicken. In conclusion, our results showed that antibiotic-induced dysbiosis of gut microbiota promotes lipogenesis of chicken by altering relevant metabolomics. The efforts in this study laid a basis for further study of the mechanisms that gut microbiota regulates lipogenesis and fat deposition of chicken.

Characterization of bovine ruminal epithelial bacterial communities using 16S rRNA sequencing, PCR-DGGE, and qRT-PCR analysis

2012 ◽  
Vol 155 (1) ◽  
pp. 72-80 ◽  
Author(s):  
Meiju Li ◽  
Mi Zhou ◽  
Elizabeth Adamowicz ◽  
John A. Basarab ◽  
Le Luo Guan
Keyword(s):  
16S Rrna ◽  
Bacterial Communities ◽  
16S Rrna Sequencing ◽  
Pcr Analysis ◽  
Qrt Pcr ◽  
Rrna Sequencing

scholarly journals The Pervasive Effects of an Antibiotic on the Human Gut Microbiota, as Revealed by Deep 16S rRNA Sequencing

PLoS Biology ◽  
2008 ◽  
Vol 6 (11) ◽  
pp. e280 ◽  
Author(s):  
Les Dethlefsen ◽  
Sue Huse ◽  
Mitchell L Sogin ◽  
David A Relman
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
16S Rrna Sequencing ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Rrna Sequencing
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