scholarly journals Search for Rpv3 and Rpv12 genes in genotypes of table and seedless grape varieties using DNA-markers

2020 ◽  
Author(s):  
M. V. Makarkina ◽  
E. T. Ilnitskaya ◽  
S. V. Tokmakov
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Dna Marker ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Marker Analysis ◽  
Seedless Grape ◽  
Seedless Grapes ◽  
Grape Varieties

DNA-marker analysis of grape genotypes for the presence of downy mildew resistance genes Rpv3 and Rpv12 was performed. Table and seedless grapes varieties and forms carring these resistance genes were identified according to the DNA-marker evaluation.

scholarly journals DNA-marker identification of Rpv3 and Rpv12 resistance loci in genotypes of table and seedless grape varieties

2020 ◽  
Vol 25 ◽  
pp. 03004
Author(s):  
Elena Ilnitskaya ◽  
Marina Makarkina ◽  
Sergey Tokmakov ◽  
Victoriya Kotlyar
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Dna Marker ◽  
Table Grape ◽  
Chemical Treatments ◽  
Marker Analysis ◽  
Seedless Grape ◽  
Special Software ◽  
Grape Varieties

DNA markers are widely used in grapevine breeding to create forms with combined resistance genes. Downy mildew is one of the most common fungal diseases of the vine in the world. Growing grapevines with increased resistance allows to reduce the number of chemical treatments. The decrease in the use of pesticides is especially significant for viticulture of table varieties, since berries are directly consumed by humans for food. Currently, more than 20 resistance genes have been identified by molecular methods, and DNA markers for many genes have been developed. The genes Rpv3 (inherited from North American grape species) and Rpv12 (derived from V. amurensis) are among the most effective and have an additive effect. The study of 14 table grape varieties for the presence of the Rpv3 gene and 8 varieties for the presence of the Rpv12 gene was performed by using DNA-marker analysis. The analysis included varieties that could inherit these genes from the parent forms, according to their ancestry. The study was conducted using an automatic genetic analyzer ABI Prism 3130 and special software GeneMapper and PeakScanner, DNA-markers were taken from literature sources. According to the results of DNA-marker analysis, 9 varieties were identified, including 2 seedless varieties, with the Rpv3299-279 allele in the genotypes, which determines resistance to downy mildew, and 3 table varieties with the Rpv12 gene in the genotypes. One table grape genotype was identified with Rpv3 and Rpv12.

scholarly journals DNA marker identification of Rpv3 downy mildew resistance gene in seedless grape varieties

2020 ◽  
Vol 2 (3) ◽  
pp. 15-19
Author(s):  
E. T. Ilnitskaya ◽  
M. V. Makarkina ◽  
S. V. Tokmakov
Keyword(s):  
Downy Mildew ◽  
Resistance Gene ◽  
Resistance Genes ◽  
North American ◽  
Dna Marker ◽  
North American Species ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Seedless Grape ◽  
Grape Varieties

Table grapes are a valuable dietary product. Seedless grapes are in high demand among consumers. For this reason, the breeding of seedless varieties is one of the popular trends in modern viticulture, along with the production of environmentally friendly products. Downy mildew (Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni) is one of the most common fungal diseases of the grapevine. Most downy mildew resistant grape accessions belong to North American species like Vitis aestivalis Michx., V. berlandieri Planch., V. cinerea (Engelm. ex A. Gray) Engelm. ex Millard, V. riparia Michx., V. rupestris  Scheele, etc. The search for donors of resistance genes is an urgent task. Rpv3 is one of the most significant resistance genes from a number of North American grape varieties. The aim of this work is to identify the downy mildew resistance gene Rpv3 in seedless grape varieties by means of DNA-marker analysis. The grape varieties with rudimentary development of seed in berries and with North American species in the pedigree were chosen as the object of the study. The varieties “Dunavski lazur” and “Seyve Villard 12-375” with reference alleles were used as the positive control, while V. vinifera L. was used as the negative control. UDV305 and UDV737 DNA-markers were used in this study to identify the allelic type of the Rpv3 gene. The work was performed using the polymerase chain reaction. The reaction products were separated by capillary electrophoresis using the ABI Prism 3130 automatic genetic analyzer. Evaluation of the results was done using the GeneMapper and PeakScanner software. Functional alleles of the downy mildew resistance gene Rpv3 were revealed in grape  varieties “Kishmish zaporozhskiy”, “Lady Patricia”, “Remaily seedless”, “Pamyati Smirnova” and “Shayan”. Rpv3299-279, one of the seven known haplotypes, was identified in all the varieties. The pedigree analysis of the studied varieties indicated that the parental forms – “Seyve Villard” and “Seibel” hybrids – are presumably the donors of the gene. Grape accessions with the identified Rpv3 gene can be used in seedless varieties breeding as donors of resistance to downy mildew.

scholarly journals SSR Markers Suitable for Marker Assisted Selection in Sunflower for Downy Mildew Resistance

2018 ◽  
Vol 13 (1) ◽  
pp. 319-326
Author(s):  
Ezgi Çabuk Şahin ◽  
Aral Kalenderoğlu ◽  
Yıldız Aydın ◽  
Göksel Evci ◽  
Ahu Altınkut Uncuoğlu
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Downy Mildew Resistance ◽  
Phenotypic Analysis ◽  
Breeding Programs ◽  
Mildew Resistance ◽  
Ssr Analysis ◽  
Genotypic Analysis ◽  
Simple Sequence

AbstractThe effectiveness of Pl genes is known to be resistant to downy mildew (DM) disease affected by fungus Plasmopara halstedii in sunflower. In this study phenotypic analysis was performed using inoculation tests and genotypic analysis were carried out with three DM resistance genes Plarg, Pl13 and Pl8. A total of 69 simple sequence repeat markers and 241 F2 individuals derived from a cross of RHA-419 (R) x P6LC (S), RHA-419 (R) x CL (S), RHA-419 (R) x OL (S), RHA419 (R) x 9758R (S), HA-R5 (R) x P6LC (S) and HA89 (R) x P6LC (S) parental lines were used to identify resistant hybrids in sunflower. Results of SSR analysis using markers linked with downy mildew resistance genes (Plarg, Pl8 and Pl13) and downy mildew inoculation tests were evaluated together and ORS716 (for Plarg and Pl13), HA4011 (for Pl8) markers showed positive correlation with their phenotypic results. These results suggest that these markers are associated with DM resistance and they can be used successfully in marker-assisted selection for sunflower breeding programs specific for downy mildew resistance.

DNA markers for downy mildew resistance genes in sorghum

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 823-826 ◽  
Author(s):  
P. S. Bhavanishankara Gowda ◽  
Richard A. Frederiksen ◽  
Clint W. Magill ◽  
Guo-Wei Xu
Keyword(s):  
Downy Mildew ◽  
Resistance Gene ◽  
Dna Markers ◽  
Fragment Length Polymorphism ◽  
Mapping Population ◽  
Random Amplified Polymorphic Dna ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Sorghum Line ◽  
Rflp Rapd

The random amplified polymorphic DNA technique was used to find markers for a downy mildew resistance gene in sorghum. Of the 674 random primers screened for polymorphism, 2 amplified fragments were linked to a downy mildew resistance gene in sorghum line SC414. Utilization of an existing restriction fragment length polymorphism mapping population (IS3620C × BTx623) also revealed two markers that are linked to a different resistance gene in another sorghum line, BTx623.Key words: sorghum, downy mildew, RFLP, RAPD.

scholarly journals Development of Sequence Characterized Amplified Region Markers Linked to Downy Mildew Resistance in Broccoli

2002 ◽  
Vol 127 (4) ◽  
pp. 597-601 ◽  
Author(s):  
Janel L. Giovannelli ◽  
Mark W. Farnham ◽  
Min Wang ◽  
Allan E. Strand
Keyword(s):  
Downy Mildew ◽  
Genetic Markers ◽  
Resistance Genes ◽  
Rapd Markers ◽  
Dominant Gene ◽  
Screening Tools ◽  
Resistance Locus ◽  
Downy Mildew Resistance ◽  
Scar Markers ◽  
Mildew Resistance

Downy mildew, caused by the fungal parasite Peronospora parasitica (Pers.: Fr.) Fr., is a destructive disease of Brassica oleracea L. crops, including broccoli (B. oleracea, Italica Group). The development and deployment of downy mildew resistant broccoli cultivars is a priority for breeders and producers. Identification of genetic markers linked to downy mildew resistance genes should facilitate selection for resistance and pyramiding of resistance genes into cultivars. The objectives of this study were to 1) identify RAPD markers linked to a single dominant gene for resistance in broccoli, 2) clone and sequence the linked RAPD markers, and 3) develop and evaluate SCAR markers as screening tools for resistance. Bulked segregant analysis led to the identification of eight linked RAPD markers following a screen of 848 decamers. Two of the linked RAPD fragments, UBC359620 and OPM16750, were converted to dominant SCAR markers linked in coupling to the resistance locus at 6.7 and 3.3 cM, respectively. The SCAR marker based on UBC359620 sequence exhibited less accuracy (94%) than the original RAPD (96%) in differentiating resistant and susceptible plants, but the accuracy (97%) of the OPM16750-SCAR was not different than the original RAPD. These SCAR markers are among the first genetic markers found linked to a gene conferring cotyledon-stage downy mildew resistance in B. oleracea. Results of this work provide breeders with useful information and tools for the systematic development of resistant cultivars.

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