Linkage of downy mildew resistance genes Pd 1 and Pd 2from Allium roylei Stearn in progeny of its interspecific hybrid with onion (A. cepa L.)

Euphytica ◽  
1992 ◽  
Vol 64 (1-2) ◽  
pp. 131-137 ◽  
Author(s):  
J. N. de Vries ◽  
W. A. Wietsma ◽  
M. C. Jongerius
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Interspecific Hybrid ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Allium Roylei

scholarly journals Молекулярные маркеры в селекции лука репчатого

2019 ◽  
Author(s):  
R.R. Alizhanova ◽  
S.G. Monakhos ◽  
G.F. Monakhos
Keyword(s):  
Male Sterility ◽  
Downy Mildew ◽  
Resistance Gene ◽  
Nuclear Gene ◽  
Gene Marker ◽  
Downy Mildew Resistance ◽  
Vegetable Crops ◽  
Mildew Resistance ◽  
Allium Roylei ◽  
Onion Downy Mildew

Исследования проведены в 2016–2018 годах в лаборатории генетики, селекции и биотехнологии овощных культур РГАУ-МСХА им. К.А. Тимирязева. Целью исследования являлась оценка эффективности известных молекулярных маркеров различных типов цитоплазмы CMS-S, CMS-T и N, гена закрепителя стерильности и восстановителя фертильности и гена, контролирующего устойчивость к пероноспорозу. Рекомендованные в научной литературе молекулярные маркеры orfA501 и 5» cob позволяют дифференцировать растения лука репчатого по трем типам цитоплазмы – CMS-S, CMS-T и N-цитоплазма. В нашем исследовании с использованием этих двух маркерных систем установлены типы цитоплазмы всех проанализированных растений с мужской стерильностью. У шести образцов выявлена CMS-S цитоплазма, у четырех образцов CMS-T и два образца обладали нормальной (N) цитоплазмой. Для создания линий закрепителей стерильности необходимо использование образцов с N-цитоплазмой, так как они должны обладать генотипом Nmsms. При классическом способе используют анализирующие скрещивания, где в качестве тестера берут стерильные растения с генотипом Smsms или Тmsms. И по потомству полученных F1 гибридов судят о генотипе опылителя. Это требует как минимум 4 года работы. C помощью маркера Jnurf 13 проводят генотипирование на аллельное состояние локуса закрепления стерильности (msms) у растений с N-цитоплазмой, что сокращает процесс создания закрепителей стерильности в 4 раза. Доказано, что полная устойчивость Allium roylei к пероноспорозу контролируется одним доминантным геном Pd1. Маркер DMR1 выявляет наличие гена устойчивости Pd1 к ложной мучнистой росе. ПЦР-анализ с маркером DMR1 показал, что у устойчивого гибрида F1 Santero ген устойчивости находится в гетерозиготном состоянии, у восприимчивых сортов Денсити и Кенди – в рецессивной гомозиготы. В потомствах растений Sx (SxДенс) и Sx (SxКенди) наблюдали расщепление на устойчивые и восприимчивые, а среди устойчивых выявлены гомо- и гетерозиготные по гену Pd1.The studies were conducted in 2016–2018 in the laboratory of genetics, breeding and biotechnology of vegetable crops of the RSAU – MTAA. The aim of the research is to assess the efficiency of known molecular markers orfA501 и 5» cob to genotype a type of onion (Allium cepa L.) male sterility inducing cytoplasm’s, CMS- (S) and CMS- (T) and normal (N) cytoplasm, to genotype restorer fertility and maintainer sterility alleles Ms/ms of a nuclear gene, marker of onion downy mildew resistance gene. The use of these two marker systems (orfA501 и 5» cob) confirmed the type of cytoplasm of all screened male sterility onion plants. Six onion accessions had CMS-S type of cytoplasm, four accessions – CMS-T and two accessions had normal N-cytoplasm. The efficiency of recommended DNA-marker JnurF13 to genotype restorer fertility (Ms) allele and maintainer sterility (ms) allele of a nuclear gene was confirmed using a set of different onion accessions. The use of molecular marker Jnurf 13 helps to genotype the onion plant in relation to allelic state of the sterility maintainer allele (ms) in plants with N-cytoplasm and shortens the process of developing sterility maintainer lines. Allium roylei is completely resistant to downy mildew which is controlled by a single dominant Pd1 gene. Marker DMR1 of onion downy mildew resistance gene was studied using an accessions of Timofeev’s plant breeding station (RSAU – MTAA) involved in a breeding programs and commercial cultivars of foreign companies.

scholarly journals SSR Markers Suitable for Marker Assisted Selection in Sunflower for Downy Mildew Resistance

2018 ◽  
Vol 13 (1) ◽  
pp. 319-326
Author(s):  
Ezgi Çabuk Şahin ◽  
Aral Kalenderoğlu ◽  
Yıldız Aydın ◽  
Göksel Evci ◽  
Ahu Altınkut Uncuoğlu
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Downy Mildew Resistance ◽  
Phenotypic Analysis ◽  
Breeding Programs ◽  
Mildew Resistance ◽  
Ssr Analysis ◽  
Genotypic Analysis ◽  
Simple Sequence

AbstractThe effectiveness of Pl genes is known to be resistant to downy mildew (DM) disease affected by fungus Plasmopara halstedii in sunflower. In this study phenotypic analysis was performed using inoculation tests and genotypic analysis were carried out with three DM resistance genes Plarg, Pl13 and Pl8. A total of 69 simple sequence repeat markers and 241 F2 individuals derived from a cross of RHA-419 (R) x P6LC (S), RHA-419 (R) x CL (S), RHA-419 (R) x OL (S), RHA419 (R) x 9758R (S), HA-R5 (R) x P6LC (S) and HA89 (R) x P6LC (S) parental lines were used to identify resistant hybrids in sunflower. Results of SSR analysis using markers linked with downy mildew resistance genes (Plarg, Pl8 and Pl13) and downy mildew inoculation tests were evaluated together and ORS716 (for Plarg and Pl13), HA4011 (for Pl8) markers showed positive correlation with their phenotypic results. These results suggest that these markers are associated with DM resistance and they can be used successfully in marker-assisted selection for sunflower breeding programs specific for downy mildew resistance.

scholarly journals Search for Rpv3 and Rpv12 genes in genotypes of table and seedless grape varieties using DNA-markers

2020 ◽  
Author(s):  
M. V. Makarkina ◽  
E. T. Ilnitskaya ◽  
S. V. Tokmakov
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Dna Marker ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Marker Analysis ◽  
Seedless Grape ◽  
Seedless Grapes ◽  
Grape Varieties

DNA-marker analysis of grape genotypes for the presence of downy mildew resistance genes Rpv3 and Rpv12 was performed. Table and seedless grapes varieties and forms carring these resistance genes were identified according to the DNA-marker evaluation.

scholarly journals Development of Sequence Characterized Amplified Region Markers Linked to Downy Mildew Resistance in Broccoli

2002 ◽  
Vol 127 (4) ◽  
pp. 597-601 ◽  
Author(s):  
Janel L. Giovannelli ◽  
Mark W. Farnham ◽  
Min Wang ◽  
Allan E. Strand
Keyword(s):  
Downy Mildew ◽  
Genetic Markers ◽  
Resistance Genes ◽  
Rapd Markers ◽  
Dominant Gene ◽  
Screening Tools ◽  
Resistance Locus ◽  
Downy Mildew Resistance ◽  
Scar Markers ◽  
Mildew Resistance

Downy mildew, caused by the fungal parasite Peronospora parasitica (Pers.: Fr.) Fr., is a destructive disease of Brassica oleracea L. crops, including broccoli (B. oleracea, Italica Group). The development and deployment of downy mildew resistant broccoli cultivars is a priority for breeders and producers. Identification of genetic markers linked to downy mildew resistance genes should facilitate selection for resistance and pyramiding of resistance genes into cultivars. The objectives of this study were to 1) identify RAPD markers linked to a single dominant gene for resistance in broccoli, 2) clone and sequence the linked RAPD markers, and 3) develop and evaluate SCAR markers as screening tools for resistance. Bulked segregant analysis led to the identification of eight linked RAPD markers following a screen of 848 decamers. Two of the linked RAPD fragments, UBC359620 and OPM16750, were converted to dominant SCAR markers linked in coupling to the resistance locus at 6.7 and 3.3 cM, respectively. The SCAR marker based on UBC359620 sequence exhibited less accuracy (94%) than the original RAPD (96%) in differentiating resistant and susceptible plants, but the accuracy (97%) of the OPM16750-SCAR was not different than the original RAPD. These SCAR markers are among the first genetic markers found linked to a gene conferring cotyledon-stage downy mildew resistance in B. oleracea. Results of this work provide breeders with useful information and tools for the systematic development of resistant cultivars.

scholarly journals QTL Mapping linked to downy mildew resistance genes in maize (Zea mays)

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
Muh Dzulkifly Ashan ◽  
MIFTAHUDIN MIFTAHUDIN ◽  
REFLINUR REFLINUR ◽  
MARCIA B. PABENDON ◽  
SIGIT BUDI SANTOSO ◽  
...  
Keyword(s):  
Zea Mays ◽  
Qtl Mapping ◽  
Downy Mildew ◽  
Resistance Genes ◽  
Average Distance ◽  
Chromosome 1 ◽  
Downy Mildew Resistance ◽  
Maize Breeding ◽  
Entire Genome ◽  
Mildew Resistance

Abstract. Ashan MD, Miftahudin, Reflinur, Pabendon MB, Santoso SB, Salim A. 2020. QTL Mapping linked to downy mildew resistance genes in maize (Zea mays). Biodiversitas 21: 3735-3743. Downy Mildew (DM) caused by Peronosclerospora spp. is one of the most destructive diseases and problems in yield losses of maize production worldwide. Identification of molecular marker-linked to the DM resistance genes is considered as an important step in the improvement of the DM resistance nature in maize breeding program. The objective of the study was to identify molecular markers linked to the DM resistance genes in maize. A total of 198 F3 maize lines generated from a cross between R10-4430 and Kandora was used in microsatellite genetic map construction and the corresponding F3: 4 families were used in phenotypic evaluation to identify quantitative trait loci (QTLs) responsible for DM resistance-related trait. The entire genetic linkage map constructed with 28 SSR markers resulted in 460.3 cM with an average distance between markers of 26.85 cM. Seven main-effect QTLs controlling the DM resistance genes were identified in the entire genome map of F3 population with the phenotypic variation explained (PVE) values ranged from 43.35 to 53.71%. Among detected QTLs, three QTLs, qDM-Pp1a, qDM-Pp1b1, and qDM-Pp1b2 were detected on chromosome 1, one QTL, qDM-Pp5 was on chromosome 5, two QTLs, qDM-Pp6b1 and qDM-Pp6b2 were on chromosome 6 and one QTL, qDM-Pp10 was on chromosome 10. The effect of detected QTLs responsible for DM resistance trait ranged from 43.35 to 53.71% and those will be potential to be further used as molecular aided selection in maize breeding for DM resistance.

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