scholarly journals Recombinant Bacillus subtilis strain deficient in production of surfactin loses ability to induce resistance of wheat plants against aphid Schizaphis graminum (Rond.)

2020 ◽  
Author(s):  
V. Yu. Alekseev ◽  
S. Veselova ◽  
D. K. Blagova ◽  
E. R. Sarvarova ◽  
G. Burkhanova ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Endophytic Bacteria ◽  
Schizaphis Graminum ◽  
Systemic Resistance ◽  
Subtilis Strain ◽  
Cereal Aphids ◽  
Insecticidal Effect ◽  
Induce Resistance ◽  
Recombinant Bacillus Subtilis

An important role of surfactin synthesis by endophytic bacteria in protecting wheat against cereal aphids has been shown to manifest itself in a direct insecticidal effect and an indirect effect through the induction of systemic resistance in plants.

scholarly journals Lipopeptide producing endophytic bacteria of the genus Bacillus regulate wheat resistance to cereal aphids

2020 ◽  
Author(s):  
S. V. Veselova ◽  
V. Yu. Alekseev ◽  
S.D. Rumyantsev ◽  
G.F. F. Burkhanova ◽  
E. A. Cherepanova ◽  
...  
Keyword(s):  
Indirect Effect ◽  
Endophytic Bacteria ◽  
Schizaphis Graminum ◽  
Systemic Resistance ◽  
Cereal Aphids ◽  
Genus Bacillus

We have shown the direct and indirect effect of lipopeptide-producing endophytic bacteria of the genus Bacillus on the viability of greenbug aphid Schizaphis graminum Rond. and the induction of systemic resistance in wheat plants.

scholarly journals Application of Endophytic Bacillus subtilis and Salicylic Acid to Improve Wheat Growth and Tolerance under Combined Drought and Fusarium Root Rot Stresses

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1343 ◽  
Author(s):  
Oksana Lastochkina ◽  
Darya Garshina ◽  
Chulpan Allagulova ◽  
Kristina Fedorova ◽  
Igor Koryakov ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Salicylic Acid ◽  
Root Rot ◽  
Endophytic Bacteria ◽  
Wheat Yield ◽  
Maximum Effect ◽  
Subtilis Strain ◽  
Signal Molecule ◽  
Wheat Growth ◽  
Root And Shoot Length

In nature, plants are constantly exposed to a varied abiotic and biotic stresses or their combinations, limiting the productivity of major crops, including wheat. Combinations of drought and soil-borne Fusarium-instigated diseases are the most common combinations of stresses, significantly reducing wheat yield around the world. Here, were analyzed the potential of application of endophytic bacteria Bacillus subtilis (strain 10–4) together with the natural signal molecule salicylic acid (SA) to improve growth and tolerance of Triticum aestivum L. (wheat) plants under combined drought and Fusarium culmorum-instigated root rot (FRR) stresses. It was revealed that pre-sowing treatment with B. subtilis 10–4, SA, and B. subtilis 10–4 + SA, both under normal and combined drought conditions, notably reduced (by 50–80% or more) the incidence of FRR development in wheat plants, with the most notable effect for B. subtilis 10–4 + SA (wherein disease symptoms were almost absent). Moreover, B. subtilis 10–4, SA, and especially B. subtilis 10–4 + SA increased plant growth (root and shoot length, fresh and dry biomass) under normal (up to 20–50%), drought (up to 15–40%), FRR (up to 15–30%), and combined drought + FRR stresses (up to 20%), with the maximum effect for B. subtilis 10–4 + SA. Additionally, B. subtilis 10–4, SA, and B. subtilis 10–4 + SA decreased stress (drought, FRR, and combined drought + FRR)-instigated lipid peroxidation and osmotic damages of plant cells. The findings indicate that endophytic bacteria B. subtilis 10–4 alone and in a mixture with SA may be used as an effective eco-friendly agent to improve wheat growth and tolerance under the influence of drought, FRR, and combinations of these stresses.

Biosynthesis of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant Bacillus subtilis strain

Microbiology ◽  
2006 ◽  
Vol 75 (2) ◽  
pp. 142-147 ◽  
Author(s):  
Yu. M. Kirillova ◽  
E. O. Mikhailova ◽  
N. P. Balaban ◽  
A. M. Mardanova ◽  
A. R. Kayumov ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Serine Proteinase ◽  
Subtilis Strain ◽  
Bacillus Intermedius ◽  
Recombinant Bacillus Subtilis

The Regulation of Bacillus intermedius Glutamyl Endopeptidase Biosynthesis in the Recombinant Bacillus subtilis Strain AJ73 during Sporulation

Microbiology ◽  
2004 ◽  
Vol 73 (3) ◽  
pp. 279-285
Author(s):  
I. B. Chastukhina ◽  
M. R. Sharipova ◽  
L. A. Gabdrakhmanova ◽  
N. P. Balaban ◽  
D. R. Safina ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Subtilis Strain ◽  
Glutamyl Endopeptidase ◽  
Bacillus Intermedius ◽  
Recombinant Bacillus Subtilis

scholarly journals Induction of Systemic Resistance to Botrytis cinerea in Tomato by Pseudomonas aeruginosa 7NSK2: Role of Salicylic Acid, Pyochelin, and Pyocyanin

2002 ◽  
Vol 15 (11) ◽  
pp. 1147-1156 ◽  
Author(s):  
Kris Audenaert ◽  
Theresa Pattery ◽  
Pierre Cornelis ◽  
Monica Höfte
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Salicylic Acid ◽  
Botrytis Cinerea ◽  
Induced Resistance ◽  
Mammalian Cells ◽  
Cell Damage ◽  
Systemic Resistance ◽  
Wild Type ◽  
Induce Resistance

The rhizobacterium Pseudomonas aeruginosa 7NSK2 produces secondary metabolites such as pyochelin (Pch), its precursor salicylic acid (SA), and the phenazine compound pyocyanin. Both 7NSK2 and mutant KMPCH (Pch-negative, SA-positive) induced resistance to Botrytis cinerea in wild-type but not in transgenic NahG tomato. SA-negative mutants of both strains lost the capacity to induce resistance. On tomato roots, KMPCH produced SA and induced phenylalanine ammonia lyase activity, while this was not the case for 7NSK2. In 7NSK2, SA is probably very efficiently converted to Pch. However, Pch alone appeared not to be sufficient to induce resistance. In mammalian cells, Fe-Pch and pyocyanin can act synergistically to generate highly reactive hydroxyl radicals that cause cell damage. Reactive oxygen species are known to play an important role in plant defense. To study the role of pyocyanin in induced resistance, a pyocyanin-negative mutant of 7NSK2, PHZ1, was generated. PHZ1 is mutated in the phzM gene encoding an O-methyltransferase. PHZ1 was unable to induce resistance to B. cinerea, whereas complementation for pyocyanin production or co-inoculation with mutant 7NSK2-562 (Pch-negative, SA-negative, pyocyanin-positive) restored induced resistance. These results suggest that pyocyanin and Pch, rather than SA, are the determinants for induced resistance in wild-type P. aeruginosa 7NSK2.

Growth conditions and production of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant Bacillus subtilis strain

Microbiology ◽  
2006 ◽  
Vol 75 (2) ◽  
pp. 136-141 ◽  
Author(s):  
Yu. M. Kirillova ◽  
E. O. Mikhailova ◽  
N. P. Balaban ◽  
A. M. Mardanova ◽  
G. N. Rudenskaya ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Serine Proteinase ◽  
Growth Conditions ◽  
Subtilis Strain ◽  
Bacillus Intermedius ◽  
Recombinant Bacillus Subtilis

scholarly journals Genetic Construction of Bacillus subtilis Recombinant Strain, Producing Anthrax Protective Antigen

2018 ◽  
Vol 2 (2) ◽  
pp. 51-60
Keyword(s):  
Bacillus Subtilis ◽  
Recombinant Strain ◽  
Protective Antigen ◽  
Shuttle Vector ◽  
Subtilis Strain ◽  
Anthrax Vaccine ◽  
Anthrax Protective Antigen ◽  
Recombinant Bacillus Subtilis ◽  
New Generation ◽  
Anthrax Vaccines

Anthrax is a serious infectious disease with high mortality. The epidemiological security depends on the vaccination of susceptible animals and population at risk. But many of the existing anthrax vaccine strains possess low levels of protective antigen production and high reactogenicity. One of the most promising trends in production of new generation of vaccines is the cloning of particular determinants of immunogenicity of anthrax microbe for the creation of highly effective producers of Bacillus anthracis protective antigen. The aim of the article is to present the results of the study on the construction of recombinant Bacillus subtilis strain, producing B.anthracis protective antigen, promising for use in chemical anthrax vaccines technology. The pHT43PA plasmid containing the gene pag, providing the synthesis of protective antigen of the anthrax microbe and functioning stably in the cells of the recombinant strain Amy21(pHT43РА) of B. subtilis, was constructed on the basis of the shuttle vector pHT43. It is found out during the research, that the microbial cells of the recombinant strain Amy21(pHT43РА) of B. subtilis provide the production of immunologically active protective antigen in quantities, not inferior than anthrax vaccine strains. These data, as well as safety and simplicity of В. subtilis make it possible to continue the research of this recombinant strain as a producer of anthrax protective antigen, promising for use in vaccines production

A new human growth hormone production process using a recombinant Bacillus subtilis strain

1991 ◽  
Vol 18 (1-2) ◽  
pp. 41-54 ◽  
Author(s):  
Elisabetta Franchi ◽  
Federico Maisano ◽  
Silvia Astrua Testori ◽  
Giuliano Galli ◽  
Salvatore Toma ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Bacillus Subtilis ◽  
Production Process ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Subtilis Strain ◽  
Hormone Production ◽  
Recombinant Bacillus Subtilis
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