scholarly journals Possible applications of conditionally immortalized tissue cell lines with differentiation functions

2003 ◽  
Vol 18 (2) ◽  
pp. 86-94
Author(s):  
Masuo Obinata ◽  
Nobuaki Yanai
Keyword(s):  
Cell Lines ◽  
Tissue Cell

Single-crystal Silicon Carbide: A Biocompatible and Hemocompatible Semiconductor for Advanced Biomedical Applications

2010 ◽  
Vol 1246 ◽  
Author(s):  
Stephen E. Saddow ◽  
Camilla Coletti ◽  
Christopher Frewin ◽  
Norelli Castro Schettini ◽  
Alexandra Oliveros ◽  
...  
Keyword(s):  
Silicon Carbide ◽  
Cell Lines ◽  
Platelet Adhesion ◽  
Crystalline Silicon ◽  
Platelet Rich Plasma ◽  
Fluorescent Microscopy ◽  
Tissue Cell ◽  
Neural Cells ◽  
Crystal Silicon

AbstractCrystalline silicon carbide (SiC) and silicon (Si) biocompatibility was evaluated in vitro by directly culturing three skin and connective tissue cell lines, two immortalized neural cell lines, and platelet-rich plasma (PRP) on these semiconducting substrates. The experiments were performed specifically for the three adopted SiC polytypes, namely 3C-, 4H- and 6H-SiC, and the results were compared to those obtained for Si crystals. Cell proliferation and adhesion quality were studied using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays and fluorescent microscopy. For the neural cells studied AFM was also used to quantify the filopodia and lamellipodia extensions on the surface of the tested materials. Fluorescent microscopy was also used to assess platelet adhesion to the semiconductor surfaces where significantly lower values of platelet adhesion to 3C-SiC was observed compared to Si. The reported results show that SiC is indeed a more biocompatible substrate than Si. While there were some differences among the degree of biocompatibility of the various SiC polytypes tested, SiC appears to be a highly biocompatible material in vitro that is also somewhat hemocompatible. This extremely intriguing result appears to put SiC into a unique class of materials that is both bio- and hemo-compatible and is, to the best of our knowledge, the only semiconductor with this property.

scholarly journals Matching cell lines with cancer type and subtype of origin via mutational, epigenomic and transcriptomic patterns

2019 ◽  
Author(s):  
Marina Salvadores ◽  
Francisco Fuster-Tormo ◽  
Fran Supek
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Experimental Work ◽  
Drug Sensitivity ◽  
Tissue Cell ◽  
Cancer Type ◽  
Tumor Type ◽  
Cell Type ◽  
Cancer Subtypes ◽  
The One

AbstractCell lines are commonly used as cancer models. Because the tissue and/or cell type of origin provide important context for understanding mechanisms of cancer, we systematically examined whether cell lines exhibit features matching the cancer type that supposedly originated them. To this end, we aligned the mRNA expression and DNA methylation data between ∼9,000 solid tumors and ∼600 cell lines to remove the global differences stemming from growth in cell culture. Next, we created classification models for cancer type and subtype using tumor data, and applied them to cell line data. Overall, the transcriptomic and epigenomic classifiers consistently identified 35 cell lines which better matched a different tissue or cell type than the one the cell line was originally annotated with; we recommend caution in using these cell lines in experimental work. Six cell lines were identified as originating from the skin, of which five were further corroborated by the presence of a UV-like mutational signature in their genome, strongly suggesting mislabelling. Overall, genomic evidence additionally supports that 22 (3.6% of all considered) cell lines may be mislabelled because we predict they originate from a different tissue/cell type. Finally, we cataloged 366 cell lines in which both transcriptomic and epigenomic profiles strongly resemble the tumor type of origin, designating them as ‘golden set’ cell lines. We suggest these cell lines are better suited for experimental work that depends on tissue identity and propose tentative assignments to cancer subtypes. Finally, we show that accounting for the uncertain tissue-of-origin labels can change the interpretation of drug sensitivity and CRISPR genetic screening data. In particular, in brain, lung and pancreatic cancer cell lines, many novel determinants of drug sensitivity or resistance emerged by focussing on the cell lines that are best matched to the cancer type of interest.

Newly synthesized glycoconjugates from two cell lines derived from rat osteogenic sarcoma: effects of Matrigenin activity from bone

1991 ◽  
Vol 69 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Ravi K. Chopra ◽  
Tassos Anastassiades ◽  
Christian Stephens ◽  
David Lohnes ◽  
Glenville Jones
Keyword(s):  
Cell Lines ◽  
Osteogenic Sarcoma ◽  
Distinct Species ◽  
Enzymatic Digestion ◽  
Proteoglycan Synthesis ◽  
Tissue Cell ◽  
Osteoblastic Cell ◽  
Bovine Bone ◽  
The Media ◽  
Umr 106

An activity isolated from bovine bone was previously shown to stimulate proteoglycan synthesis by several connective tissue cell lines from normal tissues (Matrigenin activity). The effect of this activity on glycoconjugate synthesis by two osteoblastic cell lines, ROS 17/2 and UMR-106, derived from rat osteogenic sarcoma, was examined after labelling of the cells with [3H]glucosamine and [35S]sulfate. The glycoconjugates from the cell layers and the media were separated by DEAE-Sephacel chromatography and the anionic glycoconjugates of the media were further analyzed by chromatography on Sepharose CL-2B and enzymatic digestion of the papain-released glycosaminoglycans. The ROS 17/2 cells secreted at least two distinct species of proteoglycan (one heparan sulfate rich and the other chondroitin sulfate rich), whereas the UMR-106 secreted primarily an anionic glycoprotein. The addition of Matrigenin activity to the ROS 17/2 cells resulted in stimulation of incorporation of radioactivity into the proteoglycan and hyaluronic acid, but in UMR-106 cultures it resulted in decreased incorporation into the anionic glycoprotein. The decrease in incorporation into the anionic glycoprotein from the medium was shown, by alkaline β-elimination, to have occurred mainly in the oligosaccharide fraction, relative to control cultures.Key words: osteogenic sarcoma cells, Matrigenin activity, glycoconjugate synthesis.

Unveiling the Role of MicroRNA155 in Diffuse Large B Cell Lymphoma.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 2024-2024
Author(s):  
Deepak Rai ◽  
Shailaja Karanti ◽  
Patricia Dahia ◽  
Ricardo C.T. Aguiar
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
B Cell ◽  
Cell Lines ◽  
Mhc Class Ii ◽  
B Cell Lymphoma ◽  
Tissue Cell ◽  
Transcription Analysis ◽  
Mrna Cleavage

Abstract MicroRNAs (miR) are non-coding RNAs that regulate gene expression by pairing to 3UTRs of target genes inducing translational repression or mRNA cleavage. New evidence suggests that the latter mechanism markedly contributes to miRNA effects. Hence, global gene expression analyses may help elucidate the functional role of miRNA by recognizing pathways modified by their abnormal expression and identifying direct targets. MiR155, the product of the non coding gene BIC, is overexpressed in lymphomas and its role in tumorigenesis is supported by the development of B-cell malignancies in miR155 transgenic mice. However, the functional consequences of miR155 overexpression in tumor development remain unclear. To address this issue, we developed a semi-quantitative RT-PCR assay that specifically amplifies either the nuclear unspliced BIC mRNA (target of the RNase III Drosha) or the spliced BIC mRNA. We found a marked correlation between the expression levels of these two mRNAs, which in turn agreed with the levels of mature miR155 detected in northern blots. Of the 22 DLBCL cell lines studied, only 5 (DHL6, Ly3, Ly10, Farage, RCK-8) expressed significantly high levels of BIC and miR155. To isolate the effects of miR155 in DLBCL we genetically modified its expression and performed global transcription analysis on microarray. In brief, we cloned the BIC transcript in a MSCV-GFP bicistronic retrovirus and confirmed in transduced HeLa cells that the mature miR155 was expressed when this transcript was driven by an LTR promoter. Next, we used two DLBCL cell lines with low levels of miR155 (Ly8 and Ly19) to generate unique populations expressing miR155 or MSCV alone. RNA was isolated from GFP-sorted cells, hybridized to the Affymetrix U133Plus2.0 chip and the data analyzed with dChip. Remarkably, and in agreement with the role of miRNAs, supervised analysis (fold diff >1.7) revealed a vast predominance (>90%) of downregulated genes when comparing miR155 expressing cells to MSCV only. These gene groups included predicted miR155 targets and were significantly enriched for molecules involved in the immune response (p<.001), including MHC class II, chemokine receptors, TDT, NFAT and CD24, a particularly relevant target for miR155 inhibition since its activation induces apoptosis in lymphomas. To validate and extend our findings, we queried public expression datasets of primary DLBCL. First, we used our cell lines expression data to confirm that the BIC probe in the Affymetrix chip reliably reflected the expression of miR155. Since DLBCL entails at least two groups of tumors reflecting distinct normal B-cells (GC and ABC) and the expression of miRNA and its targets are highly tissue/cell specific, we compartmentalized these analyses within the groups of ABC and GC tumors. We selected the tumors within each group with the highest and lowest levels of BIC (20% percentile) and performed unsupervised hierarchical clustering analysis (filtering parameters 0.5<SD/mean<10). In agreement with our data in the GC-type miR155-expressing cell lines, we found that in GC-, but not in ABC tumors, the expression of BIC inversely correlated with that of the genes related to the immune response. Notably, the pro-apoptotic molecule CD24 was significantly downregulated (p<0.02) in BIC overexpressing primary DLBCL, underscoring the need for further characterization of the signals relayed by this surface molecule and its potential as a rational drug target. Our data start to delineate the effects of miR155 in DLBCL and show the potential of expression arrays to identify miRNA targets modified by mRNA cleavage.

Autoclaving: a modification in the preparation of tissue culture medium 199

1972 ◽  
Vol 18 (2) ◽  
pp. 272-273 ◽  
Author(s):  
C. P. Kenny ◽  
B. B. Diena ◽  
L. Greenberg
Keyword(s):  
Tissue Culture ◽  
Rhesus Monkey ◽  
Cell Lines ◽  
Hela Cells ◽  
Culture Medium ◽  
Kidney Tissue ◽  
Monkey Kidney ◽  
Tissue Culture Medium ◽  
Tissue Cell

HeLa cells and rhesus monkey kidney tissue cell lines can be propagated and maintained in medium 199 that has been sterilized by autoclaving. The choice of autoclaved M 199 is advantageous for some experimental procedures.

Single-Crystal Silicon Carbide: A Biocompatible and Hemocompatible Semiconductor for Advanced Biomedical Applications

2011 ◽  
Vol 679-680 ◽  
pp. 824-830 ◽  
Author(s):  
Stephen E. Saddow ◽  
Christopher L. Frewin ◽  
Camilla Coletti ◽  
N. Schettini ◽  
E. Weeber ◽  
...  
Keyword(s):  
Silicon Carbide ◽  
Cell Lines ◽  
Platelet Adhesion ◽  
Crystalline Silicon ◽  
Fluorescent Microscopy ◽  
Wild Type Mouse ◽  
System Response ◽  
Tissue Cell

Crystalline silicon carbide (SiC) and silicon (Si) biocompatibility was evaluated in vitro by directly culturing three skin and connective tissue cell lines, two immortalized neural cell lines, and platelet-rich plasma (PRP) on these semiconducting substrates. The in vivo biocompatibility was then evaluated via implantation of 3C-SiC and Si shanks into a C57/BL6 wild type mouse. The in vivo results, while preliminary, were outstanding with Si being almost completely enveloped with activated microglia and astrocytes, indicating a severe immune system response, while the 3C-SiC film was virtually untouched. The in vitro experiments were performed specifically for the three adopted SiC polytypes, namely 3C-, 4H- and 6H-SiC, and the results were compared to those obtained for Si crystals. Cell proliferation and adhesion quality were studied using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays and fluorescent microscopy. The neural cells were studied via atomic force microscopy (AFM) which was used to quantify filopodia and lamellipodia extensions on the surface of the tested materials. Fluorescent microscopy was used to assess platelet adhesion to the semiconductor surfaces where significantly lower values of platelet adhesion to 3C-SiC was observed compared to Si. The reported results show good indicators that SiC is indeed a more biocompatible substrate than Si. While there were some differences among the degree of biocompatibility of the various SiC polytypes tested, SiC appears to be a highly biocompatible material in vitro that is also somewhat hemocompatible. This extremely intriguing result appears to put SiC into a unique class of materials that is both bio- and hemo-compatible and is, to the best of our knowledge, the only semiconductor with this property.

Enhancement of prostaglandin synthesizing activity by the treatment with sodium n-butyrate on cloned mastocytoma cells and various other tissue cell lines

1980 ◽  
Vol 19 (5) ◽  
pp. 659-669 ◽  
Author(s):  
Mieko Kawamura ◽  
Yasuko Koshihara ◽  
Tatsuo Senshu ◽  
Sei-itsu Murota
Keyword(s):  
Cell Lines ◽  
Tissue Cell ◽  
Mastocytoma Cells
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